Journal: Cancer Medicine

Article Title: Role of Ca 2+ ‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/ EDNRB

doi: 10.1002/cam4.71754

Figure Lengend Snippet: REG Iα promotes cell migration, invasion, and EMT via EDNRB. (A) Cell migration was assessed by Transwell assay. (B) Matrigel‐coated Transwell assay assessed cell invasion. (C) Western blot analysis of EMT‐related markers, including the epithelial marker E‐Cadherin and mesenchymal markers N‐Cadherin and Vimentin. β‐actin was used as the loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: CHOP (Abcam, ab317378), Cleaved‐Caspase 3 (Abcam, ab32042), Cleaved‐PARP (Cell Signaling Technology, 9541), E‐Cadherin (BOSTER, PB9561), N‐Cadherin (BOSTER, A01577‐3), and Vimentin (BOSTER, BM4029).

Techniques: Migration, Transwell Assay, Western Blot, Marker, Control

Journal: Cancer Medicine

Article Title: Role of Ca 2+ ‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/ EDNRB

doi: 10.1002/cam4.71754

Figure Lengend Snippet: The REG Iα‐EDNRB axis promotes cell migration, invasion, and EMT via the Ca 2+ signaling pathway. (A) Transwell assay assessed cell migration. (B) Cell invasion was assessed by Matrigel‐coated Transwell assay. (C) EMT‐related protein levels (E‐Cadherin, N‐Cadherin, Vimentin) were detected by Western blot. β‐actin served as the loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: CHOP (Abcam, ab317378), Cleaved‐Caspase 3 (Abcam, ab32042), Cleaved‐PARP (Cell Signaling Technology, 9541), E‐Cadherin (BOSTER, PB9561), N‐Cadherin (BOSTER, A01577‐3), and Vimentin (BOSTER, BM4029).

Techniques: Migration, Transwell Assay, Western Blot, Control

Journal: Cancer Medicine

Article Title: Role of Ca 2+ ‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/ EDNRB

doi: 10.1002/cam4.71754

Figure Lengend Snippet: The REG Iα‐EDNRB‐Ca 2+ axis promotes tumor growth and EMT in vivo. (A) Representative images of the xenograft tumors and excised tumor tissues from the indicated groups. (B) Statistical analysis of the final tumor weights. (C) Tumor growth curves measuring tumor volume over time. (D) H&E staining showed pathological changes in tumor tissues. (E) TUNEL assay detected cell apoptosis in tumor tissues. (F) Cell proliferation in tumor tissues was shown by Ki67 IHC staining. (G) Expression of REG Iα (Immunofluorescence, upper row) and EDNRB (IHC, lower row) in tumor tissues. (H) Western blot analysis of REG Iα, EDNRB, and p‐CaMKII protein levels in tumor tissues. β‐actin was used as the loading control. (I) Western blot analysis of EMT‐related proteins (E‐Cadherin, N‐Cadherin, Vimentin) in tumor tissues. β‐actin was used as the loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: CHOP (Abcam, ab317378), Cleaved‐Caspase 3 (Abcam, ab32042), Cleaved‐PARP (Cell Signaling Technology, 9541), E‐Cadherin (BOSTER, PB9561), N‐Cadherin (BOSTER, A01577‐3), and Vimentin (BOSTER, BM4029).

Techniques: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing, Immunofluorescence, Western Blot, Control

Journal: Diagnostics (Basel, Switzerland)

Article Title: Detection of Cadherin 12 in Plasma and Peritoneal Fluid Among Women with Endometriosis Using Novel Surface Plasmon Resonance Imaging (SPRi) Method.

doi: 10.3390/diagnostics15111366

Figure Lengend Snippet: Figure 1. Correlation of plasma CDH12 concentrations [ng/mL] with age.

Article Snippet: The following antibodies were used in the study: monoclonal recombinant rat antiCDH12 antibody (catalog no. MAB2240) and recombinant human CDH12 protein (catalog no. 2240-CA), each >95% pure (R&D Systems, Minneapolis, MN, USA), cysteamine hydrochloride, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Merck, Darmstadt, Germany), N-hydroxysuccinimide (NHS) (Merck, Darmstadt, Germany), 0.2 M carbonate buffer pH = 8.50 (Merck, Darmstadt, Germany) and Tween-20 (Merck, Darmstadt, Germany).

Techniques: Clinical Proteomics

Journal: Diagnostics (Basel, Switzerland)

Article Title: Detection of Cadherin 12 in Plasma and Peritoneal Fluid Among Women with Endometriosis Using Novel Surface Plasmon Resonance Imaging (SPRi) Method.

doi: 10.3390/diagnostics15111366

Figure Lengend Snippet: Figure 2. Concentrations of CDH12 [ng/mL] in plasma and peritoneal fluid.

Article Snippet: The following antibodies were used in the study: monoclonal recombinant rat antiCDH12 antibody (catalog no. MAB2240) and recombinant human CDH12 protein (catalog no. 2240-CA), each >95% pure (R&D Systems, Minneapolis, MN, USA), cysteamine hydrochloride, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Merck, Darmstadt, Germany), N-hydroxysuccinimide (NHS) (Merck, Darmstadt, Germany), 0.2 M carbonate buffer pH = 8.50 (Merck, Darmstadt, Germany) and Tween-20 (Merck, Darmstadt, Germany).

Techniques: Clinical Proteomics