Journal: British Journal of Pharmacology
Article Title: Angiotensin-(1-7) inhibits allergic inflammation, via the MAS1 receptor, through suppression of ERK1/2- and NF-?B-dependent pathways
Figure Lengend Snippet: Western blot analysis of p-IκB-α and p-ERK1/2 protein levels from lungs of PBS-challenged mice pretreated with vehicle (PBS), from ovalbumin (OVA)-challenged mice pretreated with vehicle (OVA), Ang-(1–7) and A779 plus Ang-(1–7).
Article Snippet: Chemicals used included ovalbumin (grade V), halothane, absolute ethanol (Merck KGaA, Darmstadt, Germany), formaldehyde (Surechem Products LTD, Suffolk, UK), Alu-Gel-S (SERVA Electrophoresis GmbH, Heidelberg, Germany), isotone II diluent solution (Beckman Coulter Inc., Krefeld, Germany), Zap-OGLOBIN (Coulter Electronics LTD, Buckinghamshire, UK), Diff-Quik (Baxter Dade AG, Dudingen, Switzerland), PBS (Sigma-Aldrich, St Louis, MO, USA), Ang-(1–7), A779, Tris-base, NaCl, Na4 P2 O7 , NaF, CaCl2 , MgCl2 , Glycerol, NP4 O, Na3 VO4 , PMSF, protease inhibitor cocktail, Ponceau 2R (Sigma-Aldrich), phosphatase inhibitor cocktail (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) xylene, ethanol (Honeywell Riedel-de Haën®, Seelze, Germany), haematoxylin, eosin, glacial acetic acid, ferric chloride, HCl, periodic acid, DMSO (BDH Laboratory Supplies, Poole, UK), Dpx mountant, picric acid, mercuric chloride, phosphomolybdic acid, light green SF yellowish, Schiff's reagent (Sigma-Aldrich), acid fuchsin (Gurr Microscopy Materials; BDH Ltd., Poole, UK), rabbit monoclonal antibodies for total and phosphorylated IκB-α and rabbit polyclonal antibody for phosphorylated ERK1/2 (Cell Signaling Technology, Inc., Danvers, MA, USA), rabbit monoclonal anti-actin antibody, phenanthroline (Sigma-Aldrich), nitrocellulose membranes (Schleicher & Schuell, Dassel, Germany).
Techniques: Western Blot, Mouse Assay