cacl2 Search Results


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  • 89
    Millipore cacl 2
    Effect of Divalent Crosslinks in ASC Microbead on Growth Factor mRNA Levels and Production. (A) mRNA levels and (B) growth factor secretion/DNA content on day 7 from ASC microbeads crosslinked in <t>CaCl2</t> Ca++) or BaCl2 (Ba++), cultured in growth medium and normalized to ASCs on tissue culture polystyrene (TCPS). (C) Growth factor retained within microbeads normalized to DNA content (n = 6 samples, mean±SE, *p
    Cacl 2, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co cacl 2
    Effect of Divalent Crosslinks in ASC Microbead on Growth Factor mRNA Levels and Production. (A) mRNA levels and (B) growth factor secretion/DNA content on day 7 from ASC microbeads crosslinked in <t>CaCl2</t> Ca++) or BaCl2 (Ba++), cultured in growth medium and normalized to ASCs on tissue culture polystyrene (TCPS). (C) Growth factor retained within microbeads normalized to DNA content (n = 6 samples, mean±SE, *p
    Cacl 2, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    PerkinElmer ca cl2
    Effect of 1,2-DCB and benzene on the initial rates of ATP hydrolysis supported by A23187. The reaction was performed in a buffer containing 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM <t>CaCl2,</t> and 1.5 μM A23187. The reaction was conducted by adding 0.01 mg/ml of SR vesicles with different concentrations of 1,2-DCB (mM) or benzene (mM). First data point corresponds to Ca 2+ -ATPase activity without DCB (control) and it was ~2.6 ± 0.05 μmol P i /mg∙min
    Ca Cl2, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ca cl2 - by Bioz Stars, 2020-08
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    92
    Fisher Scientific cacl2
    Effect of 1,2-DCB and benzene on the initial rates of ATP hydrolysis supported by A23187. The reaction was performed in a buffer containing 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM <t>CaCl2,</t> and 1.5 μM A23187. The reaction was conducted by adding 0.01 mg/ml of SR vesicles with different concentrations of 1,2-DCB (mM) or benzene (mM). First data point corresponds to Ca 2+ -ATPase activity without DCB (control) and it was ~2.6 ± 0.05 μmol P i /mg∙min
    Cacl2, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 622 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck KGaA cacl2
    Effect of 1,2-DCB and benzene on the initial rates of ATP hydrolysis supported by A23187. The reaction was performed in a buffer containing 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM <t>CaCl2,</t> and 1.5 μM A23187. The reaction was conducted by adding 0.01 mg/ml of SR vesicles with different concentrations of 1,2-DCB (mM) or benzene (mM). First data point corresponds to Ca 2+ -ATPase activity without DCB (control) and it was ~2.6 ± 0.05 μmol P i /mg∙min
    Cacl2, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cacl2 - by Bioz Stars, 2020-08
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    92
    Mediatech cacl2
    Effect of 1,2-DCB and benzene on the initial rates of ATP hydrolysis supported by A23187. The reaction was performed in a buffer containing 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM <t>CaCl2,</t> and 1.5 μM A23187. The reaction was conducted by adding 0.01 mg/ml of SR vesicles with different concentrations of 1,2-DCB (mM) or benzene (mM). First data point corresponds to Ca 2+ -ATPase activity without DCB (control) and it was ~2.6 ± 0.05 μmol P i /mg∙min
    Cacl2, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Avantor cacl2
    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with <t>CaCl2</t> ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.
    Cacl2, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Haemonetics cacl2
    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with <t>CaCl2</t> ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.
    Cacl2, supplied by Haemonetics, used in various techniques. Bioz Stars score: 90/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher cacl2
    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with <t>CaCl2</t> ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.
    Cacl2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 9860 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    FUJIFILM cacl2
    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with <t>CaCl2</t> ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.
    Cacl2, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 93/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    HiMedia Laboratories cacl2
    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with <t>CaCl2</t> ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.
    Cacl2, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Applichem cacl2
    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with <t>CaCl2</t> ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.
    Cacl2, supplied by Applichem, used in various techniques. Bioz Stars score: 93/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Dade Behring cacl2
    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with <t>CaCl2</t> ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.
    Cacl2, supplied by Dade Behring, used in various techniques. Bioz Stars score: 92/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Sinopharm cacl2
    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with <t>CaCl2</t> ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.
    Cacl2, supplied by Sinopharm, used in various techniques. Bioz Stars score: 93/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Euromedex cacl2
    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with <t>CaCl2</t> ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.
    Cacl2, supplied by Euromedex, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Bioreagents cacl2
    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with <t>CaCl2</t> ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.
    Cacl2, supplied by Fisher Bioreagents, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nacalai cacl2
    FE-SEM images of the surfaces and cross-sections of GUMMETAL ® (a) untreated, (b) subjected to NaOH, <t>CaCl2,</t> heat and water treatment (BioGum) and (c) subsequently soaked in SBF for 7 days. AFM images of (d) Ti-36Nb-2Ta-3Zr-0.3O alloy untreated and (e) NaOH, CaCl 2 , heat and water treated surfaces. TF-XRD profiles of (f) surfaces of Ti-36Nb-2Ta-3Zr-0.3O alloy: (i) untreated, (ii) subjected to NaOH, CaCl 2 , heat and water treatment and (iii) subsequently soaked in SBF for 7 days.
    Cacl2, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    GE Healthcare cacl2
    Isolation of Bothrops neuwiedi venom fractions enriched in SVMPs. (A) Samples of 50 mg of B. neuwiedi venom were applied on Hiprep 16/60 S200 column, equilibrated with 20 mM Tris, 150 mM NaCl and 1 mM CaCl 2 pH 7.8. Fractions of 2.0 mL were collected at a flow rate of 0.5 mL/min, monitored at A280 (nm). (B,C and D) Fractions I, II and IV of size exclusion chromatography were applied to a Mono-Q HR 5/5, equilibrated with 20 mM Tris pH 7.8 plus 1 mM <t>CaCl2.</t> Fractions of 1.0 mL were collected at a flow rate of 1.0 mL/min, monitored at A280 (nm).
    Cacl2, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 2774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Carl Roth GmbH cacl2
    Isolation of Bothrops neuwiedi venom fractions enriched in SVMPs. (A) Samples of 50 mg of B. neuwiedi venom were applied on Hiprep 16/60 S200 column, equilibrated with 20 mM Tris, 150 mM NaCl and 1 mM CaCl 2 pH 7.8. Fractions of 2.0 mL were collected at a flow rate of 0.5 mL/min, monitored at A280 (nm). (B,C and D) Fractions I, II and IV of size exclusion chromatography were applied to a Mono-Q HR 5/5, equilibrated with 20 mM Tris pH 7.8 plus 1 mM <t>CaCl2.</t> Fractions of 1.0 mL were collected at a flow rate of 1.0 mL/min, monitored at A280 (nm).
    Cacl2, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 93/100, based on 305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega cacl2
    Isolation of Bothrops neuwiedi venom fractions enriched in SVMPs. (A) Samples of 50 mg of B. neuwiedi venom were applied on Hiprep 16/60 S200 column, equilibrated with 20 mM Tris, 150 mM NaCl and 1 mM CaCl 2 pH 7.8. Fractions of 2.0 mL were collected at a flow rate of 0.5 mL/min, monitored at A280 (nm). (B,C and D) Fractions I, II and IV of size exclusion chromatography were applied to a Mono-Q HR 5/5, equilibrated with 20 mM Tris pH 7.8 plus 1 mM <t>CaCl2.</t> Fractions of 1.0 mL were collected at a flow rate of 1.0 mL/min, monitored at A280 (nm).
    Cacl2, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 2129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    S D Fine-Chem cacl2
    Isolation of Bothrops neuwiedi venom fractions enriched in SVMPs. (A) Samples of 50 mg of B. neuwiedi venom were applied on Hiprep 16/60 S200 column, equilibrated with 20 mM Tris, 150 mM NaCl and 1 mM CaCl 2 pH 7.8. Fractions of 2.0 mL were collected at a flow rate of 0.5 mL/min, monitored at A280 (nm). (B,C and D) Fractions I, II and IV of size exclusion chromatography were applied to a Mono-Q HR 5/5, equilibrated with 20 mM Tris pH 7.8 plus 1 mM <t>CaCl2.</t> Fractions of 1.0 mL were collected at a flow rate of 1.0 mL/min, monitored at A280 (nm).
    Cacl2, supplied by S D Fine-Chem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ICN Biomedicals cacl2
    Isolation of Bothrops neuwiedi venom fractions enriched in SVMPs. (A) Samples of 50 mg of B. neuwiedi venom were applied on Hiprep 16/60 S200 column, equilibrated with 20 mM Tris, 150 mM NaCl and 1 mM CaCl 2 pH 7.8. Fractions of 2.0 mL were collected at a flow rate of 0.5 mL/min, monitored at A280 (nm). (B,C and D) Fractions I, II and IV of size exclusion chromatography were applied to a Mono-Q HR 5/5, equilibrated with 20 mM Tris pH 7.8 plus 1 mM <t>CaCl2.</t> Fractions of 1.0 mL were collected at a flow rate of 1.0 mL/min, monitored at A280 (nm).
    Cacl2, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of Divalent Crosslinks in ASC Microbead on Growth Factor mRNA Levels and Production. (A) mRNA levels and (B) growth factor secretion/DNA content on day 7 from ASC microbeads crosslinked in CaCl2 Ca++) or BaCl2 (Ba++), cultured in growth medium and normalized to ASCs on tissue culture polystyrene (TCPS). (C) Growth factor retained within microbeads normalized to DNA content (n = 6 samples, mean±SE, *p

    Journal: Journal of Stem Cells & Regenerative Medicine

    Article Title: Adipose Stem Cell Microbeads as Production Sources for Chondrogenic Growth Factors

    doi:

    Figure Lengend Snippet: Effect of Divalent Crosslinks in ASC Microbead on Growth Factor mRNA Levels and Production. (A) mRNA levels and (B) growth factor secretion/DNA content on day 7 from ASC microbeads crosslinked in CaCl2 Ca++) or BaCl2 (Ba++), cultured in growth medium and normalized to ASCs on tissue culture polystyrene (TCPS). (C) Growth factor retained within microbeads normalized to DNA content (n = 6 samples, mean±SE, *p

    Article Snippet: In studies determining growth factor production and secretion from ASC microbeads over time and in studies determining the effect of chondrogenic medium and microencapsulation on ASCs from multiple donors, microbeads with 130 kDa, 44% guluronate alginate (LVM, low viscosity high mannuronate) were crosslinked in a solution of 50 mM CaCl2 (Sigma, St. Louis, MO, USA), 150 mM glucose (Sigma) and 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Sigma) at pH 7.3 for at least 15 minutes.

    Techniques: Cell Culture

    Effect of 1,2-DCB and benzene on the initial rates of ATP hydrolysis supported by A23187. The reaction was performed in a buffer containing 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM CaCl2, and 1.5 μM A23187. The reaction was conducted by adding 0.01 mg/ml of SR vesicles with different concentrations of 1,2-DCB (mM) or benzene (mM). First data point corresponds to Ca 2+ -ATPase activity without DCB (control) and it was ~2.6 ± 0.05 μmol P i /mg∙min

    Journal: BMC Biochemistry

    Article Title: 1,2-Dichlorobenzene affects the formation of the phosphoenzyme stage during the catalytic cycle of the Ca2+-ATPase from sarcoplasmic reticulum

    doi: 10.1186/s12858-016-0061-1

    Figure Lengend Snippet: Effect of 1,2-DCB and benzene on the initial rates of ATP hydrolysis supported by A23187. The reaction was performed in a buffer containing 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM CaCl2, and 1.5 μM A23187. The reaction was conducted by adding 0.01 mg/ml of SR vesicles with different concentrations of 1,2-DCB (mM) or benzene (mM). First data point corresponds to Ca 2+ -ATPase activity without DCB (control) and it was ~2.6 ± 0.05 μmol P i /mg∙min

    Article Snippet: Chemicals and reagents [γ-32 P]ATP and 45 CaCl2 were purchased from Perkin-Elmer, USA.

    Techniques: Activity Assay

    Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with CaCl2 ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.

    Journal: Scientific Reports

    Article Title: A new cell-laden 3D Alginate-Matrigel hydrogel resembles human breast cancer cell malignant morphology, spread and invasion capability observed “in vivo”

    doi: 10.1038/s41598-018-23250-4

    Figure Lengend Snippet: Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with CaCl2 ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.

    Article Snippet: Gels were gently removed from the molds, transferred into a 96 multi-well plate and maintained in complete medium containing 5 mM CaCl2 .

    Techniques: Multiple Displacement Amplification, Concentration Assay

    FE-SEM images of the surfaces and cross-sections of GUMMETAL ® (a) untreated, (b) subjected to NaOH, CaCl2, heat and water treatment (BioGum) and (c) subsequently soaked in SBF for 7 days. AFM images of (d) Ti-36Nb-2Ta-3Zr-0.3O alloy untreated and (e) NaOH, CaCl 2 , heat and water treated surfaces. TF-XRD profiles of (f) surfaces of Ti-36Nb-2Ta-3Zr-0.3O alloy: (i) untreated, (ii) subjected to NaOH, CaCl 2 , heat and water treatment and (iii) subsequently soaked in SBF for 7 days.

    Journal: Journal of Tissue Engineering

    Article Title: Improved bioactivity of GUMMETAL®, Ti59Nb36Ta2Zr3O0.3, via formation of nanostructured surfaces

    doi: 10.1177/2041731418774178

    Figure Lengend Snippet: FE-SEM images of the surfaces and cross-sections of GUMMETAL ® (a) untreated, (b) subjected to NaOH, CaCl2, heat and water treatment (BioGum) and (c) subsequently soaked in SBF for 7 days. AFM images of (d) Ti-36Nb-2Ta-3Zr-0.3O alloy untreated and (e) NaOH, CaCl 2 , heat and water treated surfaces. TF-XRD profiles of (f) surfaces of Ti-36Nb-2Ta-3Zr-0.3O alloy: (i) untreated, (ii) subjected to NaOH, CaCl 2 , heat and water treatment and (iii) subsequently soaked in SBF for 7 days.

    Article Snippet: The SBF was prepared by dissolving reagent grade NaCl, NaHCO3 , KCl, K2 HPO4 ·3H2 O, MgCl·6H2 O, CaCl2 and Na2 SO4 (Nacalai Tesque Inc., Kyoto, Japan) in ultrapure water and buffered at pH = 7.4 with tris(hydroxymethyl)aminomethane (CH2 OH)3 CNH2 and 1 M HCl (Nacalai Tesque Inc., Kyoto, Japan) at 36.5°C.

    Techniques:

    Isolation of Bothrops neuwiedi venom fractions enriched in SVMPs. (A) Samples of 50 mg of B. neuwiedi venom were applied on Hiprep 16/60 S200 column, equilibrated with 20 mM Tris, 150 mM NaCl and 1 mM CaCl 2 pH 7.8. Fractions of 2.0 mL were collected at a flow rate of 0.5 mL/min, monitored at A280 (nm). (B,C and D) Fractions I, II and IV of size exclusion chromatography were applied to a Mono-Q HR 5/5, equilibrated with 20 mM Tris pH 7.8 plus 1 mM CaCl2. Fractions of 1.0 mL were collected at a flow rate of 1.0 mL/min, monitored at A280 (nm).

    Journal: PLoS ONE

    Article Title: Functional Variability of Snake Venom Metalloproteinases: Adaptive Advantages in Targeting Different Prey and Implications for Human Envenomation

    doi: 10.1371/journal.pone.0109651

    Figure Lengend Snippet: Isolation of Bothrops neuwiedi venom fractions enriched in SVMPs. (A) Samples of 50 mg of B. neuwiedi venom were applied on Hiprep 16/60 S200 column, equilibrated with 20 mM Tris, 150 mM NaCl and 1 mM CaCl 2 pH 7.8. Fractions of 2.0 mL were collected at a flow rate of 0.5 mL/min, monitored at A280 (nm). (B,C and D) Fractions I, II and IV of size exclusion chromatography were applied to a Mono-Q HR 5/5, equilibrated with 20 mM Tris pH 7.8 plus 1 mM CaCl2. Fractions of 1.0 mL were collected at a flow rate of 1.0 mL/min, monitored at A280 (nm).

    Article Snippet: The fractions containing metalloproteinase activities (hemorrhagic and fibrinolytic) were pooled according to major peaks and dialyzed against 20 mM Tris, 1 mM CaCl2 , pH 7.8 and applied to a Mono-Q HR 5/5 column (GE Healthcare) using the same buffer.

    Techniques: Isolation, Flow Cytometry, Size-exclusion Chromatography