Journal: Scientific Reports

Article Title: A new cell-laden 3D Alginate-Matrigel hydrogel resembles human breast cancer cell malignant morphology, spread and invasion capability observed “in vivo”

doi: 10.1038/s41598-018-23250-4

Figure Lengend Snippet: Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with CaCl2 ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.

Article Snippet: Meanwhile, an agar solution at 1% (w/v) concentration was prepared by mixing Agar (DIFCO Laboratories) in physiological buffer enriched with 0.5 M of CaCl2 (J. T. Baker).

Techniques: Multiple Displacement Amplification, Concentration Assay

Journal: Journal of Tissue Engineering

Article Title: Improved bioactivity of GUMMETAL®, Ti59Nb36Ta2Zr3O0.3, via formation of nanostructured surfaces

doi: 10.1177/2041731418774178

Figure Lengend Snippet: FE-SEM images of the surfaces and cross-sections of GUMMETAL ® (a) untreated, (b) subjected to NaOH, CaCl2, heat and water treatment (BioGum) and (c) subsequently soaked in SBF for 7 days. AFM images of (d) Ti-36Nb-2Ta-3Zr-0.3O alloy untreated and (e) NaOH, CaCl 2 , heat and water treated surfaces. TF-XRD profiles of (f) surfaces of Ti-36Nb-2Ta-3Zr-0.3O alloy: (i) untreated, (ii) subjected to NaOH, CaCl 2 , heat and water treatment and (iii) subsequently soaked in SBF for 7 days.

Article Snippet: The SBF was prepared by dissolving reagent grade NaCl, NaHCO3 , KCl, K2 HPO4 ·3H2 O, MgCl·6H2 O, CaCl2 and Na2 SO4 (Nacalai Tesque Inc., Kyoto, Japan) in ultrapure water and buffered at pH = 7.4 with tris(hydroxymethyl)aminomethane (CH2 OH)3 CNH2 and 1 M HCl (Nacalai Tesque Inc., Kyoto, Japan) at 36.5°C.

Techniques: