Journal: Journal of Stem Cells & Regenerative Medicine

Article Title: Adipose Stem Cell Microbeads as Production Sources for Chondrogenic Growth Factors

doi:

Figure Lengend Snippet: Effect of Divalent Crosslinks in ASC Microbead on Growth Factor mRNA Levels and Production. (A) mRNA levels and (B) growth factor secretion/DNA content on day 7 from ASC microbeads crosslinked in CaCl2 Ca++) or BaCl2 (Ba++), cultured in growth medium and normalized to ASCs on tissue culture polystyrene (TCPS). (C) Growth factor retained within microbeads normalized to DNA content (n = 6 samples, mean±SE, *p

Article Snippet: In studies determining growth factor production and secretion from ASC microbeads over time and in studies determining the effect of chondrogenic medium and microencapsulation on ASCs from multiple donors, microbeads with 130 kDa, 44% guluronate alginate (LVM, low viscosity high mannuronate) were crosslinked in a solution of 50 mM CaCl2 (Sigma, St. Louis, MO, USA), 150 mM glucose (Sigma) and 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Sigma) at pH 7.3 for at least 15 minutes.

Techniques: Cell Culture

Journal: BMC Biochemistry

Article Title: 1,2-Dichlorobenzene affects the formation of the phosphoenzyme stage during the catalytic cycle of the Ca2+-ATPase from sarcoplasmic reticulum

doi: 10.1186/s12858-016-0061-1

Figure Lengend Snippet: Effect of 1,2-DCB and benzene on the initial rates of ATP hydrolysis supported by A23187. The reaction was performed in a buffer containing 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM CaCl2, and 1.5 μM A23187. The reaction was conducted by adding 0.01 mg/ml of SR vesicles with different concentrations of 1,2-DCB (mM) or benzene (mM). First data point corresponds to Ca 2+ -ATPase activity without DCB (control) and it was ~2.6 ± 0.05 μmol P i /mg∙min

Article Snippet: Chemicals and reagents [γ-32 P]ATP and 45 CaCl2 were purchased from Perkin-Elmer, USA.

Techniques: Activity Assay

Journal: Journal of Tissue Engineering

Article Title: Improved bioactivity of GUMMETAL®, Ti59Nb36Ta2Zr3O0.3, via formation of nanostructured surfaces

doi: 10.1177/2041731418774178

Figure Lengend Snippet: FE-SEM images of the surfaces and cross-sections of GUMMETAL ® (a) untreated, (b) subjected to NaOH, CaCl2, heat and water treatment (BioGum) and (c) subsequently soaked in SBF for 7 days. AFM images of (d) Ti-36Nb-2Ta-3Zr-0.3O alloy untreated and (e) NaOH, CaCl 2 , heat and water treated surfaces. TF-XRD profiles of (f) surfaces of Ti-36Nb-2Ta-3Zr-0.3O alloy: (i) untreated, (ii) subjected to NaOH, CaCl 2 , heat and water treatment and (iii) subsequently soaked in SBF for 7 days.

Article Snippet: The SBF was prepared by dissolving reagent grade NaCl, NaHCO3 , KCl, K2 HPO4 ·3H2 O, MgCl·6H2 O, CaCl2 and Na2 SO4 (Nacalai Tesque Inc., Kyoto, Japan) in ultrapure water and buffered at pH = 7.4 with tris(hydroxymethyl)aminomethane (CH2 OH)3 CNH2 and 1 M HCl (Nacalai Tesque Inc., Kyoto, Japan) at 36.5°C.

Techniques:

Journal: Scientific Reports

Article Title: A new cell-laden 3D Alginate-Matrigel hydrogel resembles human breast cancer cell malignant morphology, spread and invasion capability observed “in vivo”

doi: 10.1038/s41598-018-23250-4

Figure Lengend Snippet: Composite cell-laden gel development. ( A ) Schematic description of the protocol used to produce 3D cell-laden Alginate-Matrigelcomposite gels: breast cancer cells MDA-MB-231 are seeded first in liquid alginate and then Matrigel is added working on ice. The cell-laden solution is transferred into Agar molds enriched with CaCl2 ions at 37 °C for to allow gelation. Then, 3D gels are removed by molds and trasferred into plates with culture media. ( B ) Gels composed by 25%:75% A:M and 100% M ratios resulted too soft and not handy. ( C ) a concentration of 2 million cells/ml caused a fast degradation of 50%:50% A:M gels, thus it was reduced to 1 million cells/ml. ( D ) finally, structurally compact gels were obtained.

Article Snippet: Gels were gently removed from the molds, transferred into a 96 multi-well plate and maintained in complete medium containing 5 mM CaCl2 .

Techniques: Multiple Displacement Amplification, Concentration Assay

Journal: PLoS ONE

Article Title: Functional Variability of Snake Venom Metalloproteinases: Adaptive Advantages in Targeting Different Prey and Implications for Human Envenomation

doi: 10.1371/journal.pone.0109651

Figure Lengend Snippet: Isolation of Bothrops neuwiedi venom fractions enriched in SVMPs. (A) Samples of 50 mg of B. neuwiedi venom were applied on Hiprep 16/60 S200 column, equilibrated with 20 mM Tris, 150 mM NaCl and 1 mM CaCl 2 pH 7.8. Fractions of 2.0 mL were collected at a flow rate of 0.5 mL/min, monitored at A280 (nm). (B,C and D) Fractions I, II and IV of size exclusion chromatography were applied to a Mono-Q HR 5/5, equilibrated with 20 mM Tris pH 7.8 plus 1 mM CaCl2. Fractions of 1.0 mL were collected at a flow rate of 1.0 mL/min, monitored at A280 (nm).

Article Snippet: The fractions containing metalloproteinase activities (hemorrhagic and fibrinolytic) were pooled according to major peaks and dialyzed against 20 mM Tris, 1 mM CaCl2 , pH 7.8 and applied to a Mono-Q HR 5/5 column (GE Healthcare) using the same buffer.

Techniques: Isolation, Flow Cytometry, Size-exclusion Chromatography