Journal: RSC Advances

Article Title: An electrochemically synthesized molecularly imprinted polymer for highly selective detection of breast cancer biomarker CA 15-3: a promising point-of-care biosensor †

doi: 10.1039/d4ra02051k

Figure Lengend Snippet: Selectivity studies using a competitive method between the target molecule and the interfering species. The interfering species studied were CEA, CA 125, and urea.

Article Snippet: Potassium hexacyano trihydrate (K 4 Fe(CN) 6 ·3H 2 O), potassium hexacyano dihydrate (K 3 Fe(CN) 6 ), sodium phosphate dibasic dihydrate (Na 2 HPO 4 ·2H 2 O) and sodium dihydrogen phosphate dihydrate (NaH 2 PO 4 ·2H 2 O) were purchased from Riedel de Haën; fetal bovine serum (FBS) was purchased from Alfa Aesar; sulphuric acid (H 2 SO 4 ) was obtained from BDH; urea was from Fagron; proteinase K and (3-acrylamidopropyl)trimethylammonium chloride (solution 75 wt% in H 2 O) (AMPTMA) was acquired from Sigma-Aldrich; carcinoembryonic antigen (CEA) was obtained from EastCostBio; Cancer Antigen 125 (CA-125) was from Hytest; CA 15-3 from host human (reference MBS536585) was purchased from MyBioSource.

Techniques:

Journal: Cancer Prevention Research

Article Title: Programmable Bio-Nano-Chip Systems for Serum CA125 Quantification: Toward Ovarian Cancer Diagnostics at the Point-of-Care

doi: 10.1158/1940-6207.capr-11-0508

Figure Lengend Snippet: Figure 5. [A] Effect of detecting antibody incubation times on the slope and SNR of the CA125 p-BNCs. Decrease in incubation time resulted in improved immunoassay slope along with corresponding increase in precision shown by the error bars. [B] Effect of sample incubation flow rates on the slope of the CA 125 immunoassay. Initial increase in flow rate resulted in a steep increase in slope through 750 mL/min (optimal flow rate) for the optimal capturing antibody concentration (320 ng/bead) and then leveled off. Similar trends were also noted for other capturing antibody concentrations.

Article Snippet: Anti-CA125 monoclonal antibodies (Clones M002201, M002203, M77161, M8072320, M8072321, andM8072322) were obtained from Fitzgerald Industries International.

Techniques: Incubation, Concentration Assay

Journal: Cancer Prevention Research

Article Title: Programmable Bio-Nano-Chip Systems for Serum CA125 Quantification: Toward Ovarian Cancer Diagnostics at the Point-of-Care

doi: 10.1158/1940-6207.capr-11-0508

Figure Lengend Snippet: Figure 6. [A] Dose-response curve for CA125 quantification on the p-BNCs over 10 to 400 U/mL concentration range. [B] Plot showing good correlation between the p-BNCs and FDA-approved ELISA for values of CA125 measured in the sera of patients with advanced- stage epithelial ovarian cancer.

Article Snippet: Anti-CA125 monoclonal antibodies (Clones M002201, M002203, M77161, M8072320, M8072321, andM8072322) were obtained from Fitzgerald Industries International.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay