Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Detection of DEN-1 in C6/36 cells infected at different MOIs on day 1 postinoculation using indirect IF stain

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Infection

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Comparison of three fixation-permeabilization methods for the detection of dengue virus in C6/36 cells using flow cytometry a

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Flow Cytometry

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Comparison of three MAbs used for the detection of DEN-1 virus (Hawaii) antigen in C6/36 cells using flow cytometry a

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Flow Cytometry

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Flow cytometric histograms of DEN-1 (Hawaii strain)-infected C6/36 cells stained with DEN-1 MAb 16-4 (anti-NS1) (black) overlaid with histograms of mock-infected cells (white). C6/36 mosquito cells were infected with DEN-1 (Hawaii) at an MOI of 0.01. (A through G) Different time points postinfection. FL1-H, FITC fluorescence intensity. The bars represent the proportion of positive cells. (H) Detection of DEN-1 viral RNA in infected C6/36 cells collected at different time points postinfection using RT-nPCR. Lanes 1 to 8 show results at 8, 12, 16, 24, 26, 30, 48, and 72 h postinfection, respectively. Lane 9, mock-infected C6/36 cells; lane 10, culture medium only; lane 11, DEN-1 (Hawaii) virus; lane 12, reagent control; lane M, molecular size markers.

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Infection, Staining, Fluorescence

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Comparison of the sensitivity in detection of DEN-1 virus (Hawaii)-infected C6/36 cells by conventional IF staining and flow cytometry a

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Staining, Flow Cytometry

Journal: Heliyon

Article Title: Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses

doi: 10.1016/j.heliyon.2018.e00736

Figure Lengend Snippet: Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 JCRB strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.

Article Snippet: The growth kinetics of flaviviruses in C6/36 JCRB strain was significantly lower than that in C6/36 ECACC strain ( A).

Techniques: Infection, Cell Culture, Genomic Sequencing, Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: Heliyon

Article Title: Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses

doi: 10.1016/j.heliyon.2018.e00736

Figure Lengend Snippet: C6/36 cells of JCRB and ECACC strains showed different susceptibility to flavivirus infection. Growth kinetics of arboviruses in C6/36 cell lines. A : C6/36 cells of either JCRB or ECACC strain were infected with Zika virus (ZIKV), Japanese encephalitis virus (JEV), or Dengue virus (DENV) at a multiplicity of infection (MOI) of 0.01, and the titers in the supernatants were determined by plaque assay. B : C6/36 cells of either JCRB or ECACC strain were infected with Getah virus (GETV) or Sindbis virus (SINV) with MOI = 0.001. Red lines show the titer in C6/36 JCRB strain and black lines show that in C6/36 ECACC strain. The experiment was triplicated, and the standard deviations are also indicated. Asterisks showed the results of t -test for the data group at each time points (* p < 0.05, ** p < 0.01).

Article Snippet: The growth kinetics of flaviviruses in C6/36 JCRB strain was significantly lower than that in C6/36 ECACC strain ( A).

Techniques: Infection, Virus, Plaque Assay

Journal: Emerging Infectious Diseases

Article Title: Enzootic Transmission of Yellow Fever Virus in Peru

doi: 10.3201/eid0908.030075

Figure Lengend Snippet: Peruvian yellow fever isolates used in this study a

Article Snippet: 149 , 3/95 , PERU95D , Pasco , Oxapampa Villa Rica , 1,000–2,000 , hf-M , SM1, C6/36#1.

Techniques: Sequencing