Journal: Scientific Reports

Article Title: Intestinal microbiota link lymphopenia to murine autoimmunity via PD-1 + CXCR5 −/dim B-helper T cell induction

doi: 10.1038/srep46037

Figure Lengend Snippet: Tc cells and Treg cells were isolated from the splenocytes of BALB/c WT mice and transferred separately or together into BALB/c nu/nu mice. Tc, n = 10, Tc + Treg, n = 10, Treg, n = 4. ( a ) Total IgM and IgG concentrations in sera from recipient nu/nu mice were determined by ELISA. ( b ) ANAs in the recipient sera 3 weeks after Tc cell-transfer were detected by immunofluorescence assay. Representative patterns of cellular staining of HEp-2 cells by sera diluted at 1:40 followed by development with Alexa 488-conjugated goat anti-mouse IgG are shown. Original magnification x400. ( c ) Titers of ANAs in the sera from each recipient 4 weeks after the transfer. ( d ) Various ANAs detected by ELISA within 4 weeks after the transfer. ( e ) Immunoprecipitation of serum antibodies to nuclear antigens. Extracts of nuclear components of spleens from WT mice were biotinylated and reacted with sera taken from WT normal control mice (NC), pristane-induced lupus model mice (Pri), or nu/nu mice 4 weeks after the transfer of Tc or Treg cells, followed by immunoprecipitation with mAbs to mouse IgG. Cumulative plot data and bars are shown as mean ± SEM from 2 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.005.

Article Snippet: Rag2 −/− BALB/c mice and TCRα −/− BALB/c mice were purchased from Taconic Farms.

Techniques: Isolation, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Immunoprecipitation

Journal: iScience

Article Title: Amyloid-β 1-42 oligomers enhance mGlu 5 R-dependent synaptic weakening via NMDAR activation and complement C5aR1 signaling

doi: 10.1016/j.isci.2023.108412

Figure Lengend Snippet:

Article Snippet: Mouse: C57BL/6JOlaHsd , Envigo , N/A.

Techniques: Recombinant, Software

Journal: Journal of Immunotoxicology

Article Title: An ELISA for detection of complement-bound circulating immune complexes in mice

doi: 10.1080/1547691x.2019.1599471

Figure Lengend Snippet: Figure 1. Preparation of the positive control. The positive control was used as quality control in the enzyme-linked immunosorbent assay (ELISA). Mouse serum con- taining mouse immunoglobulin (Ig) and inactivated complement was incubated with heat-aggregated human IgG (“Complement Activator”) to stimulate complement activation by the classical complement pathway. Monomeric mouse IgG was able to bind to aggregated human IgG, leading to formation of complement bound hIgG-mIgG complexes. HRP (horse radish peroxidase) signal.

Article Snippet: In brief, mouse serum complement (Innovative Research, Novi, MI, cat. no IMS-C57BL6-COMPL) was incubated at 37 C for 90min with 1:100 HAGG (Complement Activator, Quidel, San Diego, CA, cat. #A114), followed by inactivation by addition of ice-cold 500mM ethylenediamine-tetraacetic acid (EDTA; to final concentration of 20mM) and storage (undiluted) at 80 C until analysis.

Techniques: Positive Control, Control, Enzyme-linked Immunosorbent Assay, Incubation, Activation Assay