c2c12 cells Search Results


c2c12  (ATCC)
99
ATCC c2c12
C2c12, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12 cells  (Genecopoeia)
95
Genecopoeia c2c12 cells
C2c12 Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12 cells  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology c2c12 cells
C2c12 Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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l6  (ATCC)
96
ATCC l6
L6, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12 lysates  (Rockland Immunochemicals)
85
Rockland Immunochemicals c2c12 lysates
Forced expression of NF-YA in differentiated muscle cells inhibits the down-regulation of several cell cycle gene expression and the induction of p21, myogenin, and creatine kinase activity. <t>C2C12</t> cells were transiently transfected with a plasmid carrying NF-YA (+) or the empty vector (–) as indicated. After transfection, cells were grown in the absence of growth factor for 6, 12, 24, or 48 h and lysed to prepare whole cell extract (WCE) (A) and measured creatine kinase activity (B). WCEs from cells cultured in growth medium (GM) and in the absence of growth factor were subjected to Western blot analysis by using antibodies against the indicated proteins. (B) Creatine kinase activity was measured on extracts prepared from transfected and untrasfected cells. Results are the mean ± S.E. of three independent experiments.
C2c12 Lysates, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Forced expression of NF-YA in differentiated muscle cells inhibits the down-regulation of several cell cycle gene expression and the induction of p21, myogenin, and creatine kinase activity. C2C12 cells were transiently transfected with a plasmid carrying NF-YA (+) or the empty vector (–) as indicated. After transfection, cells were grown in the absence of growth factor for 6, 12, 24, or 48 h and lysed to prepare whole cell extract (WCE) (A) and measured creatine kinase activity (B). WCEs from cells cultured in growth medium (GM) and in the absence of growth factor were subjected to Western blot analysis by using antibodies against the indicated proteins. (B) Creatine kinase activity was measured on extracts prepared from transfected and untrasfected cells. Results are the mean ± S.E. of three independent experiments.

Journal:

Article Title: Requirement for Down-Regulation of the CCAAT-binding Activity of the NF-Y Transcription Factor during Skeletal Muscle Differentiation

doi: 10.1091/mbc.E02-09-0600

Figure Lengend Snippet: Forced expression of NF-YA in differentiated muscle cells inhibits the down-regulation of several cell cycle gene expression and the induction of p21, myogenin, and creatine kinase activity. C2C12 cells were transiently transfected with a plasmid carrying NF-YA (+) or the empty vector (–) as indicated. After transfection, cells were grown in the absence of growth factor for 6, 12, 24, or 48 h and lysed to prepare whole cell extract (WCE) (A) and measured creatine kinase activity (B). WCEs from cells cultured in growth medium (GM) and in the absence of growth factor were subjected to Western blot analysis by using antibodies against the indicated proteins. (B) Creatine kinase activity was measured on extracts prepared from transfected and untrasfected cells. Results are the mean ± S.E. of three independent experiments.

Article Snippet: The filters containing C2C12 lysates were immunoreacted with 0.3 μg/ml anti-NF-YA rabbit polyclonal antibody (Rockland), 0.1 μg/ml anti-cyclin B1 rabbit polyclonal antibody, 0.1 μg/ml anti-cyclin A rabbit polyclonal antibody, 0.1 μg/ml anti-cdk1 p34 rabbit polyclonal antibody, 1 μg/ml anti-myogenin rabbit polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA), 1:500 anti-mouse p21 (kindly provided by C. Schneider, Laboratorio Nazionale CIB, Trieste, Italy), rabbit polyclonal antibody 0.25 μg/ml anti-Hsp70 mouse mAb (StressGen, Biotechnologies), following the manufacturer's directions.

Techniques: Expressing, Activity Assay, Transfection, Plasmid Preparation, Cell Culture, Western Blot

Cyclin B2 and cyclin A promoters are devoid of sequence-specific transcription factor interactions in differentiated cells. Genomic footprinting of the coding strand of the mouse cyclin B2 and the noncoding strand of the mouse cyclin A promoters from P and TD C2C12 cells. As a control, the DMS reactivity for DNA purified from asynchronous C2C12 cells is shown (in vitro). The CCAAT boxes are indicated.

Journal:

Article Title: Requirement for Down-Regulation of the CCAAT-binding Activity of the NF-Y Transcription Factor during Skeletal Muscle Differentiation

doi: 10.1091/mbc.E02-09-0600

Figure Lengend Snippet: Cyclin B2 and cyclin A promoters are devoid of sequence-specific transcription factor interactions in differentiated cells. Genomic footprinting of the coding strand of the mouse cyclin B2 and the noncoding strand of the mouse cyclin A promoters from P and TD C2C12 cells. As a control, the DMS reactivity for DNA purified from asynchronous C2C12 cells is shown (in vitro). The CCAAT boxes are indicated.

Article Snippet: The filters containing C2C12 lysates were immunoreacted with 0.3 μg/ml anti-NF-YA rabbit polyclonal antibody (Rockland), 0.1 μg/ml anti-cyclin B1 rabbit polyclonal antibody, 0.1 μg/ml anti-cyclin A rabbit polyclonal antibody, 0.1 μg/ml anti-cdk1 p34 rabbit polyclonal antibody, 1 μg/ml anti-myogenin rabbit polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA), 1:500 anti-mouse p21 (kindly provided by C. Schneider, Laboratorio Nazionale CIB, Trieste, Italy), rabbit polyclonal antibody 0.25 μg/ml anti-Hsp70 mouse mAb (StressGen, Biotechnologies), following the manufacturer's directions.

Techniques: Sequencing, Footprinting, Purification, In Vitro

Promoter activities of NF-Y cell cycle target genes are down-regulated in differentiated muscle cells. CAT or Luc-reporter constructs carrying the cyclin B1, cdk1, cyclin A, cyclin B2, cdc25A, cdc25C, and cyclin E promoters were stably transfected by calcium-phosphate in C2C12 cells. The obtained polyclonal cell lines were analyzed for CAT or luciferase activity. The values in TD cells are expressed, on the y-axis, as percentages of the CAT or luciferase activity obtained from proliferating cells (100%). Results represent one typical experiment performed in triplicate.

Journal:

Article Title: Requirement for Down-Regulation of the CCAAT-binding Activity of the NF-Y Transcription Factor during Skeletal Muscle Differentiation

doi: 10.1091/mbc.E02-09-0600

Figure Lengend Snippet: Promoter activities of NF-Y cell cycle target genes are down-regulated in differentiated muscle cells. CAT or Luc-reporter constructs carrying the cyclin B1, cdk1, cyclin A, cyclin B2, cdc25A, cdc25C, and cyclin E promoters were stably transfected by calcium-phosphate in C2C12 cells. The obtained polyclonal cell lines were analyzed for CAT or luciferase activity. The values in TD cells are expressed, on the y-axis, as percentages of the CAT or luciferase activity obtained from proliferating cells (100%). Results represent one typical experiment performed in triplicate.

Article Snippet: The filters containing C2C12 lysates were immunoreacted with 0.3 μg/ml anti-NF-YA rabbit polyclonal antibody (Rockland), 0.1 μg/ml anti-cyclin B1 rabbit polyclonal antibody, 0.1 μg/ml anti-cyclin A rabbit polyclonal antibody, 0.1 μg/ml anti-cdk1 p34 rabbit polyclonal antibody, 1 μg/ml anti-myogenin rabbit polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA), 1:500 anti-mouse p21 (kindly provided by C. Schneider, Laboratorio Nazionale CIB, Trieste, Italy), rabbit polyclonal antibody 0.25 μg/ml anti-Hsp70 mouse mAb (StressGen, Biotechnologies), following the manufacturer's directions.

Techniques: Construct, Stable Transfection, Transfection, Luciferase, Activity Assay

NF-YA does not directly modulate the transcriptional activity of p21 and myogenin promoters. Eighty nanograms of a vector carrying p21 promoter (A) and 2 μg of a vector carrying myogenin promoter (B) have been transiently cotransfected in proliferating C2C12 cells together with the same amount of the empty vector or vector expressing wild-type or mutant (YA13m29) NF-YA proteins. As positive control, vector carrying p21 promoter has been cotransfected with an eukaryotic vector expressing p53 protein, and the luciferase activity of the vector carrying myogenin promoter has been measured in low-serum (1%) growth condition. As internal control of transfection efficiency, all samples have been cotransfected with the CMVβgal reporter construct. Values are the means ± standard deviations of four independent experiments.

Journal:

Article Title: Requirement for Down-Regulation of the CCAAT-binding Activity of the NF-Y Transcription Factor during Skeletal Muscle Differentiation

doi: 10.1091/mbc.E02-09-0600

Figure Lengend Snippet: NF-YA does not directly modulate the transcriptional activity of p21 and myogenin promoters. Eighty nanograms of a vector carrying p21 promoter (A) and 2 μg of a vector carrying myogenin promoter (B) have been transiently cotransfected in proliferating C2C12 cells together with the same amount of the empty vector or vector expressing wild-type or mutant (YA13m29) NF-YA proteins. As positive control, vector carrying p21 promoter has been cotransfected with an eukaryotic vector expressing p53 protein, and the luciferase activity of the vector carrying myogenin promoter has been measured in low-serum (1%) growth condition. As internal control of transfection efficiency, all samples have been cotransfected with the CMVβgal reporter construct. Values are the means ± standard deviations of four independent experiments.

Article Snippet: The filters containing C2C12 lysates were immunoreacted with 0.3 μg/ml anti-NF-YA rabbit polyclonal antibody (Rockland), 0.1 μg/ml anti-cyclin B1 rabbit polyclonal antibody, 0.1 μg/ml anti-cyclin A rabbit polyclonal antibody, 0.1 μg/ml anti-cdk1 p34 rabbit polyclonal antibody, 1 μg/ml anti-myogenin rabbit polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA), 1:500 anti-mouse p21 (kindly provided by C. Schneider, Laboratorio Nazionale CIB, Trieste, Italy), rabbit polyclonal antibody 0.25 μg/ml anti-Hsp70 mouse mAb (StressGen, Biotechnologies), following the manufacturer's directions.

Techniques: Activity Assay, Plasmid Preparation, Expressing, Mutagenesis, Positive Control, Luciferase, Transfection, Construct