Journal: Journal of experimental & clinical cancer research : CR

Article Title: Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer.

doi: 10.1186/s13046-022-02315-3

Figure Lengend Snippet: Fig. 8 The expression of FOXC2, STC1 and ITGB6 is associated with poor survival in OC patients. A Representative images of IHC staining of FOXC2, STC1 and ITGB6 in OC tissues. B-D OS of OC patients with different expression levels of FOXC2, STC1 and ITGB6. (Data are shown as the mean ± SD values. Significance was calculated using Student’s t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.)

Article Snippet: Primary antibodies specific for the following proteins were used in the IF experiments: STC1 (sc-293435, Santa Cruz Biotechnology, 1:100 dilution, Dallas, Texas, USA), FOXC2 (23,066–1-AP, Proteintech, 1:100 dilution, Wuhan, Hubei, China), ITGB6 (19,695–1-AP, Proteintech, 1:100 dilution, Wuhan, Hubei, China), UCP1 (AF8292, Beyotime, 1:100 dilution, Shanghai, China), TOM20 (11,802–1-AP, Proteintech, 1:100 dilution, Wuhan, Hubei, China), perilipin1 (ab172907, Abcam, 1:100 dilution, Cambridge, UK).

Techniques: Expressing, Immunohistochemistry

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer.

doi: 10.1186/s13046-022-02315-3

Figure Lengend Snippet: Fig. 9 Schematic model showing the role of the FOXC2/STC1/ITGB6 signaling axis in the regulation of metastasis, lipid metabolism and DDP chemoresistance in OC cells

Article Snippet: Primary antibodies specific for the following proteins were used in the IF experiments: STC1 (sc-293435, Santa Cruz Biotechnology, 1:100 dilution, Dallas, Texas, USA), FOXC2 (23,066–1-AP, Proteintech, 1:100 dilution, Wuhan, Hubei, China), ITGB6 (19,695–1-AP, Proteintech, 1:100 dilution, Wuhan, Hubei, China), UCP1 (AF8292, Beyotime, 1:100 dilution, Shanghai, China), TOM20 (11,802–1-AP, Proteintech, 1:100 dilution, Wuhan, Hubei, China), perilipin1 (ab172907, Abcam, 1:100 dilution, Cambridge, UK).

Techniques:

Journal: Journal of Lipid Research

Article Title: Accumulation of long-chain bases in yeast promotes their conversion to a long-chain base vinyl ether [S]

doi: 10.1194/jlr.M070748

Figure Lengend Snippet: Identification of a putative LCB derivative. A: Schematic overview of the yeast sphingolipid biosynthetic and degradative pathways. Key enzymes and lipid intermediates are shown. Mutants used in this study are indicated in bold, and drugs that were used are shown in red. The pathway leading to dihydroceramide is highlighted in orange, the maturation of phytoceramide to the complex sphingolipids is highlighted in green, and the degradative pathway is highlighted in blue. B, C: ESI/MS profile of LCBs present in wild-type (WT; B) and elo3Δ (C) mutant cells. Lipid extracts prepared from wild-type and elongase (elo3Δ) mutant cells were analyzed by ESI/MS in the positive ion mode using the odd chain-length C17-DHS (m/z 288.3) as internal standard (indicated in blue). The major PHS species present in both strains, C18-PHS (m/z 318.3) is indicated in green. The [M + H]+ ion at m/z 344.3, indicated in red, represents a putative novel LCB derivative.

Article Snippet: Sphingosine, 1-deoxysphinganine, C18- d -erythro-DHS, C2-dihydroceramide, C17/C18-DHS, and C17/C18-PHS were obtained from Avanti (Avanti Polar Lipids, Alabaster, AL); l -threo-DHS and 2-deoxyglucose were from Sigma.

Techniques: Mutagenesis

Journal: Oncotarget

Article Title: A four-gene signature predicts survival in clear-cell renal-cell carcinoma

doi: 10.18632/oncotarget.12631

Figure Lengend Snippet: A, B. Low expression of PTEN and PIK3C2A were correlated with high risk, poor prognosis and shorter OS time. C, D. High expression of ITPA and BCL3 indicated high risk, poor prognosis and shorter OS time. Kaplan-Meier survival curves were also constructed to reveal the relationship between predicted risk of ccRCC patients and the OS time. The results showed that patients with high risk had a significantly shorter OS time than those with low risk (A-D). Green and red lines indicated low- and high-risk groups, respectively. P <0.05 was considered to be statistically significant. Cens: Censored; Event: Death; Prog. Idx.: Prognosis Index.

Article Snippet: Subsequently, the sections were first incubated with protein blocker for 1 h at 37°C, and then incubated with anti-PTEN antibody (Ready-to-use, ZSGB-BIO, ZA-0251, China), anti-PIK3C2A antibody (1:50, Proteintech, 22028-1-AP, China), anti-ITPA antibody (1:50, Proteintech, 16134-1-AP, China) and anti-BCL3 antibody (1:50, Proteintech, 23959-1-AP, China) at 4°C overnight.

Techniques: Expressing, Construct

Journal: Oncotarget

Article Title: A four-gene signature predicts survival in clear-cell renal-cell carcinoma

doi: 10.18632/oncotarget.12631

Figure Lengend Snippet: IHC analysis on tissue microarrays and Kaplan-Meier survival curves were constructed to verify the relationship between PTEN A. , PIK3C2A B. , ITPA C. and BCL3 D. expression with regard to OS and DFS. (A, B) Negative expression of PTEN and PIK3C2A were correlated with shorter OS and DFS time and worse prognosis. (C, D) Positive expression of ITPA and BCL3 were correlated with shorter OS and DFS time and worse prognosis. Green and blue lines indicated positive and negative expression groups, respectively. P <0.05 was considered to be statistically significant.

Article Snippet: Subsequently, the sections were first incubated with protein blocker for 1 h at 37°C, and then incubated with anti-PTEN antibody (Ready-to-use, ZSGB-BIO, ZA-0251, China), anti-PIK3C2A antibody (1:50, Proteintech, 22028-1-AP, China), anti-ITPA antibody (1:50, Proteintech, 16134-1-AP, China) and anti-BCL3 antibody (1:50, Proteintech, 23959-1-AP, China) at 4°C overnight.

Techniques: Construct, Expressing

Journal: Oncotarget

Article Title: A four-gene signature predicts survival in clear-cell renal-cell carcinoma

doi: 10.18632/oncotarget.12631

Figure Lengend Snippet: A. SurvExpress was used to analyze the association of the four-gene signature with the predicted risk, survival time and prognosis. B. The gene expression level of PTEN, PIK3C2A, ITPA and BCL3 were detected in high risk and low risk group. C. Kaplan-Meier survival curves showed that patients with predicted high risk ( n = 219) had significantly shorter OS time than those with low risk ( n = 249) ( P <0.05). D. ROC analysis was performed to compare the sensitivity and specificity of the survival prediction between our models. P <0.05 was considered to be statistically significant. Cens: Censored; Event: Death; Prog. Idx.: Prognosis Index; Sur.(M): Survival status (Month).

Article Snippet: Subsequently, the sections were first incubated with protein blocker for 1 h at 37°C, and then incubated with anti-PTEN antibody (Ready-to-use, ZSGB-BIO, ZA-0251, China), anti-PIK3C2A antibody (1:50, Proteintech, 22028-1-AP, China), anti-ITPA antibody (1:50, Proteintech, 16134-1-AP, China) and anti-BCL3 antibody (1:50, Proteintech, 23959-1-AP, China) at 4°C overnight.

Techniques: Gene Expression

Journal: Oncotarget

Article Title: A four-gene signature predicts survival in clear-cell renal-cell carcinoma

doi: 10.18632/oncotarget.12631

Figure Lengend Snippet: Correlation between clinicopathological features and the four proteins expression in ccRCC ( n =174)

Article Snippet: Subsequently, the sections were first incubated with protein blocker for 1 h at 37°C, and then incubated with anti-PTEN antibody (Ready-to-use, ZSGB-BIO, ZA-0251, China), anti-PIK3C2A antibody (1:50, Proteintech, 22028-1-AP, China), anti-ITPA antibody (1:50, Proteintech, 16134-1-AP, China) and anti-BCL3 antibody (1:50, Proteintech, 23959-1-AP, China) at 4°C overnight.

Techniques: Expressing

Journal: Oncotarget

Article Title: A four-gene signature predicts survival in clear-cell renal-cell carcinoma

doi: 10.18632/oncotarget.12631

Figure Lengend Snippet: Univariate and multivariate Cox proportional hazard regression analyses of the association of clinicopathological characteristics and the four genes' expression levels with OS

Article Snippet: Subsequently, the sections were first incubated with protein blocker for 1 h at 37°C, and then incubated with anti-PTEN antibody (Ready-to-use, ZSGB-BIO, ZA-0251, China), anti-PIK3C2A antibody (1:50, Proteintech, 22028-1-AP, China), anti-ITPA antibody (1:50, Proteintech, 16134-1-AP, China) and anti-BCL3 antibody (1:50, Proteintech, 23959-1-AP, China) at 4°C overnight.

Techniques: Expressing

Journal: Oncotarget

Article Title: A four-gene signature predicts survival in clear-cell renal-cell carcinoma

doi: 10.18632/oncotarget.12631

Figure Lengend Snippet: Univariate and multivariate Cox proportional hazard regression analyses of the association of clinicopathological characteristics and the four genes' expression levels with DFS

Article Snippet: Subsequently, the sections were first incubated with protein blocker for 1 h at 37°C, and then incubated with anti-PTEN antibody (Ready-to-use, ZSGB-BIO, ZA-0251, China), anti-PIK3C2A antibody (1:50, Proteintech, 22028-1-AP, China), anti-ITPA antibody (1:50, Proteintech, 16134-1-AP, China) and anti-BCL3 antibody (1:50, Proteintech, 23959-1-AP, China) at 4°C overnight.

Techniques: Expressing