c18 column Search Results


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  • 90
    Thermo Fisher c18 column
    RP <t>C18</t> nanoLC separation of permethylated high mannose type and biantennary complex type N-glycans. Overlay plots of extracted ion chromatograms for the high mannose structures indicated that the Man 5–9 GlcNAc 2 itol N-glycans were well resolved from one another but not for their individual isomeric constituents. Larger complex type N-glycans, with and without sulfates, produced more complicated and not well resolved ion chromatograms, the unambiguous identification of individual chromatographic peaks based on MS 2 of the protonated molecular ions alone is not feasible but glycotopes carried on each can still be efficiently determined by MS 2 -pd-MS 3 analysis.
    C18 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3761 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 column/product/Thermo Fisher
    Average 90 stars, based on 3761 article reviews
    Price from $9.99 to $1999.99
    c18 column - by Bioz Stars, 2020-09
    90/100 stars
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    92
    Waters Corporation c18 column
    Histone preparation with Lys-propionylation, trypsin digestion, and N-terminal PIC-labeling. A , Sample preparation workflow. B , Relative ionization efficiencies H3 T3-R8 peptide in all modified forms. C , Recovery of H3 T3-R8 peptides following StageTip <t>C18</t> cleanup. All abundances are expressed relative to the unmodified peptide.
    C18 Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 7364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 column/product/Waters Corporation
    Average 92 stars, based on 7364 article reviews
    Price from $9.99 to $1999.99
    c18 column - by Bioz Stars, 2020-09
    92/100 stars
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    94
    Phenomenex c18 column
    Chromatograms of Medicago truncatula seed extract. A. Injection into a <t>C18</t> column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.
    C18 Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 94/100, based on 6929 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 column/product/Phenomenex
    Average 94 stars, based on 6929 article reviews
    Price from $9.99 to $1999.99
    c18 column - by Bioz Stars, 2020-09
    94/100 stars
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    99
    Agilent technologies zorbax sb c18 column
    Chromatograms of representative semi-preparative HPLC purification of [ 18 F]VAT. Agilent <t>Zorbax</t> <t>SB-C18</t> column, 38% acetonitrile in 62% 0.1 M ammonium formate, v/v, pH 6.5, 4 mL/min. Panel A: radiochemical trace and panel B: UV trace at 254 nm.
    Zorbax Sb C18 Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 5424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zorbax sb c18 column/product/Agilent technologies
    Average 99 stars, based on 5424 article reviews
    Price from $9.99 to $1999.99
    zorbax sb c18 column - by Bioz Stars, 2020-09
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    99
    Thermo Fisher hypersil gold c18 column
    Identification of heme O by LC-MS/MS in schizonts extracts. Heme O was separated using <t>C18</t> Vac columns and eluted with DMSO and analyzed by LC-MS / MS. ( A ) Heme O separation was performed using a <t>Hypersil</t> Gold C18 Column (see material and methods: Mass Spectrometry; LC-MS/MS) and the peak with retention time of 11.58 was analyzed by MS/MS. ( B) MS/MS of peak of 11.58 showing the m/z of heme O (arrow, 839.4, compatible with its calculated mass).
    Hypersil Gold C18 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 950 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hypersil gold c18 column/product/Thermo Fisher
    Average 99 stars, based on 950 article reviews
    Price from $9.99 to $1999.99
    hypersil gold c18 column - by Bioz Stars, 2020-09
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    92
    Waters Corporation sep pak c18 column
    The R2 fraction prepared from the ethyl acetate fraction of the roasted coffee bean extract exhibits anti-inflammatory activity. ( A ) Procedure for the fractionation of the ethyl acetate fraction of coffee bean extract using the Sep <t>Pak</t> <t>C18</t> column. ( B ) The ethyl acetate fraction of the roasted coffee bean extract ( E ) and the R1-R3 fractions were analyzed by HPLC. ( C – F ) RAW264.7 cells were pretreated with fractions R1-R3, equivalent to 5% (v/v) of coffee, for 1 h prior to the LPS stimulation (1 μg/mL). ( C ) Nitrate concentrations in culture supernatants were measured 24 h after the LPS stimulation using Griess reagent. ( D ) iNOS mRNA expression was assessed 12 h after the LPS stimulation by RT-PCR. GAPDH mRNA expression was used as an internal control. ( E ) The amounts of CCL2 in supernatants were evaluated 24 h after the LPS stimulation by ELISA. ( F ) CCL2 mRNA expression was assessed 2 h after the LPS stimulation by RT-PCR. ( G ) RAW264.7 cells were transfected with pNF-κB-Luc and pRL-TK. Transfected cells were pretreated with fractions R1-R3, equivalent to 5% (v/v) of coffee, for 1 h prior to the stimulation with LPS (1 μg/mL) for 6 h. NF-κB-dependent luciferase activity was normalized to the activity of constitutively expressed Renilla luciferase. ( H ) RAW264.7 cells were treated with fractions R1-R3, equivalent to 5% (v/v) of coffee, for 1 h. Nuclear extracts were immunoblotted with an anti-Nrf2 or anti-Lamin B antibody. The relative expression levels of Nrf2 in the nucleus are shown in the graph.
    Sep Pak C18 Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sep pak c18 column/product/Waters Corporation
    Average 92 stars, based on 837 article reviews
    Price from $9.99 to $1999.99
    sep pak c18 column - by Bioz Stars, 2020-09
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    Image Search Results


    RP C18 nanoLC separation of permethylated high mannose type and biantennary complex type N-glycans. Overlay plots of extracted ion chromatograms for the high mannose structures indicated that the Man 5–9 GlcNAc 2 itol N-glycans were well resolved from one another but not for their individual isomeric constituents. Larger complex type N-glycans, with and without sulfates, produced more complicated and not well resolved ion chromatograms, the unambiguous identification of individual chromatographic peaks based on MS 2 of the protonated molecular ions alone is not feasible but glycotopes carried on each can still be efficiently determined by MS 2 -pd-MS 3 analysis.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Advancing a High Throughput Glycotope-centric Glycomics Workflow Based on nanoLC-MS2-product Dependent-MS3 Analysis of Permethylated Glycans* *

    doi: 10.1074/mcp.TIR117.000156

    Figure Lengend Snippet: RP C18 nanoLC separation of permethylated high mannose type and biantennary complex type N-glycans. Overlay plots of extracted ion chromatograms for the high mannose structures indicated that the Man 5–9 GlcNAc 2 itol N-glycans were well resolved from one another but not for their individual isomeric constituents. Larger complex type N-glycans, with and without sulfates, produced more complicated and not well resolved ion chromatograms, the unambiguous identification of individual chromatographic peaks based on MS 2 of the protonated molecular ions alone is not feasible but glycotopes carried on each can still be efficiently determined by MS 2 -pd-MS 3 analysis.

    Article Snippet: Another EASY-nLC™ 1200 system was interfaced to an Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (ThermoFisher Scientific) via a Nanospray Flex™ Ion Sources (ThermoFisher Scientific) for nanoLC separation at 50 °C using the same 25 cm x 75 μm C18 column (Acclaim PepMap® RSLC, ThermoFisher Scientific) at a constant flow rate of 300 nL/min.

    Techniques: Produced, Mass Spectrometry

    RP C18 nanoLC separation and MS 2 /MS 3 identification of permethylated, nonfucosylated and mono-fucosylated, single LacNAc-extended core 1 and core 2 O-glycan structures. Overlay plots of extracted ion chromatograms for the nonfucosylated ( m / z 961.53) and monofucosylated ( m / z 1135.62) (Hex-HexNAc)-Hex-HexNAcitol derived from AGC and Colo205 cells are shown to demonstrate ability to resolve the smaller isomeric O-glycans. Peaks are labeled #1 - 9, the MS 2 and target MS 3 (small inset) spectra of which are shown in 9 small panels, with the diagnostic oxonium ions annotated in red. Z 1 ion for the reducing end GalNAitol (annotated in blue) could be readily detected at m / z 294 or after further loss of 6-arm substituents at m / z 280, which is informative of core 1 versus core 2 structures. A Z 2 ion at m / z 498 is also commonly observed for all core structures that are extended at either the 6 or 3-arm but not both. Extended core 1 structure further afforded a B ion at the Gal of the Gal-GalNAcitol, e.g. m / z 668 and 842, which is very useful to define the entire moiety extending from the 3-arm. Under low collision energy, protonated permethylated glycans do not normally yield cross-ring cleavage ions.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Advancing a High Throughput Glycotope-centric Glycomics Workflow Based on nanoLC-MS2-product Dependent-MS3 Analysis of Permethylated Glycans* *

    doi: 10.1074/mcp.TIR117.000156

    Figure Lengend Snippet: RP C18 nanoLC separation and MS 2 /MS 3 identification of permethylated, nonfucosylated and mono-fucosylated, single LacNAc-extended core 1 and core 2 O-glycan structures. Overlay plots of extracted ion chromatograms for the nonfucosylated ( m / z 961.53) and monofucosylated ( m / z 1135.62) (Hex-HexNAc)-Hex-HexNAcitol derived from AGC and Colo205 cells are shown to demonstrate ability to resolve the smaller isomeric O-glycans. Peaks are labeled #1 - 9, the MS 2 and target MS 3 (small inset) spectra of which are shown in 9 small panels, with the diagnostic oxonium ions annotated in red. Z 1 ion for the reducing end GalNAitol (annotated in blue) could be readily detected at m / z 294 or after further loss of 6-arm substituents at m / z 280, which is informative of core 1 versus core 2 structures. A Z 2 ion at m / z 498 is also commonly observed for all core structures that are extended at either the 6 or 3-arm but not both. Extended core 1 structure further afforded a B ion at the Gal of the Gal-GalNAcitol, e.g. m / z 668 and 842, which is very useful to define the entire moiety extending from the 3-arm. Under low collision energy, protonated permethylated glycans do not normally yield cross-ring cleavage ions.

    Article Snippet: Another EASY-nLC™ 1200 system was interfaced to an Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (ThermoFisher Scientific) via a Nanospray Flex™ Ion Sources (ThermoFisher Scientific) for nanoLC separation at 50 °C using the same 25 cm x 75 μm C18 column (Acclaim PepMap® RSLC, ThermoFisher Scientific) at a constant flow rate of 300 nL/min.

    Techniques: Mass Spectrometry, Derivative Assay, Labeling, Diagnostic Assay

    Histone preparation with Lys-propionylation, trypsin digestion, and N-terminal PIC-labeling. A , Sample preparation workflow. B , Relative ionization efficiencies H3 T3-R8 peptide in all modified forms. C , Recovery of H3 T3-R8 peptides following StageTip C18 cleanup. All abundances are expressed relative to the unmodified peptide.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title:

    doi: 10.1074/mcp.O114.046573

    Figure Lengend Snippet: Histone preparation with Lys-propionylation, trypsin digestion, and N-terminal PIC-labeling. A , Sample preparation workflow. B , Relative ionization efficiencies H3 T3-R8 peptide in all modified forms. C , Recovery of H3 T3-R8 peptides following StageTip C18 cleanup. All abundances are expressed relative to the unmodified peptide.

    Article Snippet: Peptides were loaded onto a C18 column (BEH-C18; 100 μm i.d. x 10 cm; 1.7 μm particles, 130Å pores; Waters Corp., Milford, MA) for 10 min at 1.5 μl per minute in 2% solvent B (0.1% v/v formic acid, 98% v/v acetonitrile) and separated at 1 μl per minute by a linear gradient from 2% solvent B to 25% solvent B over 60 min followed by a ramp to 40% B in 15 min, then to 90% B and re-equilibration at 2% B for a 90-min total run time.

    Techniques: Labeling, Sample Prep, Modification

    Sources of discrepancy in H3K4 quantitation. A , Equimolar mixture of isotope-labeled, propionylated H3 T3-R8 peptide in eight modification states, injected in the milieu of endogenous histones from 293T cells. B , Time course of digestion releasing propionylated H3 T3-R8 from propionylated H3 A1-R17, where K4 is trimethylated. C , Relative ionization efficiencies of the propionylated H3 T3-R8 peptide in its various modified forms. D , Recovery of H3 T3-R8 peptides following StageTip C18 cleanup. In panels A , C , D , all abundances are expressed relative to the unmodified peptide.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title:

    doi: 10.1074/mcp.O114.046573

    Figure Lengend Snippet: Sources of discrepancy in H3K4 quantitation. A , Equimolar mixture of isotope-labeled, propionylated H3 T3-R8 peptide in eight modification states, injected in the milieu of endogenous histones from 293T cells. B , Time course of digestion releasing propionylated H3 T3-R8 from propionylated H3 A1-R17, where K4 is trimethylated. C , Relative ionization efficiencies of the propionylated H3 T3-R8 peptide in its various modified forms. D , Recovery of H3 T3-R8 peptides following StageTip C18 cleanup. In panels A , C , D , all abundances are expressed relative to the unmodified peptide.

    Article Snippet: Peptides were loaded onto a C18 column (BEH-C18; 100 μm i.d. x 10 cm; 1.7 μm particles, 130Å pores; Waters Corp., Milford, MA) for 10 min at 1.5 μl per minute in 2% solvent B (0.1% v/v formic acid, 98% v/v acetonitrile) and separated at 1 μl per minute by a linear gradient from 2% solvent B to 25% solvent B over 60 min followed by a ramp to 40% B in 15 min, then to 90% B and re-equilibration at 2% B for a 90-min total run time.

    Techniques: Quantitation Assay, Labeling, Modification, Injection

    Direct comparison of standard propionylation (Prop-x2) labeling and the hybrid (Prop-PIC) labeling method via LC-MS using a 1:1 mixture of labeled histone samples. A , Extracted ion chromatograms of the hydrophilic H3T3-R8 peptide (TK 4 QTAR) and its modified versions on C18 reverse-phase LC-MS. Retention times and relative abundances are increased by the hybrid labeling method. B , Quantitative comparison of the recoveries of each H3.1 histone tail peptide, where integrated peak areas for all modification states are combined. C , Quantitative comparison of the recoveries of the H3 T3-R8 peptide in its various modification states for the Prop-PIC versus Propx2 (integrated peak areas; n = 3, error bars are standard deviations).

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title:

    doi: 10.1074/mcp.O114.046573

    Figure Lengend Snippet: Direct comparison of standard propionylation (Prop-x2) labeling and the hybrid (Prop-PIC) labeling method via LC-MS using a 1:1 mixture of labeled histone samples. A , Extracted ion chromatograms of the hydrophilic H3T3-R8 peptide (TK 4 QTAR) and its modified versions on C18 reverse-phase LC-MS. Retention times and relative abundances are increased by the hybrid labeling method. B , Quantitative comparison of the recoveries of each H3.1 histone tail peptide, where integrated peak areas for all modification states are combined. C , Quantitative comparison of the recoveries of the H3 T3-R8 peptide in its various modification states for the Prop-PIC versus Propx2 (integrated peak areas; n = 3, error bars are standard deviations).

    Article Snippet: Peptides were loaded onto a C18 column (BEH-C18; 100 μm i.d. x 10 cm; 1.7 μm particles, 130Å pores; Waters Corp., Milford, MA) for 10 min at 1.5 μl per minute in 2% solvent B (0.1% v/v formic acid, 98% v/v acetonitrile) and separated at 1 μl per minute by a linear gradient from 2% solvent B to 25% solvent B over 60 min followed by a ramp to 40% B in 15 min, then to 90% B and re-equilibration at 2% B for a 90-min total run time.

    Techniques: Labeling, Liquid Chromatography with Mass Spectroscopy, Modification

    Development of a faster FAHFA analysis method ( a ) Structures of 5-, 9-, and 12-PAHSA and 12-OAHSA. Extracted ion chromatograms showing the retention times of 5-, 9-, and 12-PAHSA and 5-, 9-, 12-OAHSA standards on a Luna C18(2) column (3 µm, 250 × 2.0 mm, Phenomenex) ( b ) and an Acquity UPLC BEH C18 column (1.7 µm, 2.1 mm × 100 mm, Waters) ( c ).

    Journal: Analytical chemistry

    Article Title: A Faster Protocol for Endogenous FAHFA Measurements

    doi: 10.1021/acs.analchem.8b00503

    Figure Lengend Snippet: Development of a faster FAHFA analysis method ( a ) Structures of 5-, 9-, and 12-PAHSA and 12-OAHSA. Extracted ion chromatograms showing the retention times of 5-, 9-, and 12-PAHSA and 5-, 9-, 12-OAHSA standards on a Luna C18(2) column (3 µm, 250 × 2.0 mm, Phenomenex) ( b ) and an Acquity UPLC BEH C18 column (1.7 µm, 2.1 mm × 100 mm, Waters) ( c ).

    Article Snippet: An Acquity UPLC BEH C18 column (1.7 µm, 2.1 mm × 100 mm, Waters) was used for separation of FAHFAs.

    Techniques:

    Purification of stingray ghrelin-like peptide (GRLN-LP) from stomach extracts . Black bars indicate the measured fluorescence changes in intracellular calcium ion concentrations in CHO cells expressing rat GHS-R1a (CHO-GHSR62). (A) Carboxymethyl (CM)-cation ion-exchange HPLC (pH 4.8) of the SP-III fraction of stomach extracts. The GRLN-like activity was divided into four groups (A-D). (B) Preparative reverse-phase (RP)-HPLC (Symmetry C18, 3.9 × 150 mm) of group B after purification with an anti-rat GRLN1-11 immuno-affinity column. (C) Final purification of the active fraction indicated in (B) by another RP-HPLC (Vydac diphenyl, 219TP5215, 2.1 × 150 mm).

    Journal: BMC Biochemistry

    Article Title: Ghrelin-like peptide with fatty acid modification and O-glycosylation in the red stingray, Dasyatis akajei

    doi: 10.1186/1471-2091-10-30

    Figure Lengend Snippet: Purification of stingray ghrelin-like peptide (GRLN-LP) from stomach extracts . Black bars indicate the measured fluorescence changes in intracellular calcium ion concentrations in CHO cells expressing rat GHS-R1a (CHO-GHSR62). (A) Carboxymethyl (CM)-cation ion-exchange HPLC (pH 4.8) of the SP-III fraction of stomach extracts. The GRLN-like activity was divided into four groups (A-D). (B) Preparative reverse-phase (RP)-HPLC (Symmetry C18, 3.9 × 150 mm) of group B after purification with an anti-rat GRLN1-11 immuno-affinity column. (C) Final purification of the active fraction indicated in (B) by another RP-HPLC (Vydac diphenyl, 219TP5215, 2.1 × 150 mm).

    Article Snippet: The samples were first applied to a preparative RP-HPLC with a Symmetry C18 column (3.9 × 150 mm, Waters) at a flow rate of 1 ml/min under a linear gradient from 10% to 60% acetonitlile containing 0.1% TFA for 40 min.

    Techniques: Purification, Fluorescence, Expressing, High Performance Liquid Chromatography, Activity Assay, Affinity Column

    U ltra-performance liquid chromatography ( UPLC) of ginsenosides in Korean red ginseng–water extract (KRG-WE). UPLC analysis was performed by an ACQUITY BEH C18 column (100 mm × 2.1 mm, 1.7 μm; Waters, Milford, MA, USA) at column temperature of 40°C. The binary gradient elution system consisted of 0.001% phosphoric acid in water and 0.001% phosphoric acid in acetonitrile.

    Journal: Journal of Ginseng Research

    Article Title: Protective effects of Korean Red Ginseng against sub-acute immobilization stress-induced testicular damage in experimental rats

    doi: 10.1016/j.jgr.2017.09.002

    Figure Lengend Snippet: U ltra-performance liquid chromatography ( UPLC) of ginsenosides in Korean red ginseng–water extract (KRG-WE). UPLC analysis was performed by an ACQUITY BEH C18 column (100 mm × 2.1 mm, 1.7 μm; Waters, Milford, MA, USA) at column temperature of 40°C. The binary gradient elution system consisted of 0.001% phosphoric acid in water and 0.001% phosphoric acid in acetonitrile.

    Article Snippet: The separation was accomplished on an ACQUITY BEH C18 column (100 mm × 2.1 mm, 1.7 μm; Waters) at a column temperature of 40°C.

    Techniques: Liquid Chromatography

    Single-nucleotide separation of RNAs up to 59 nt by HPLC. (A) Individual chromatogram for SynAN57, SynAN58 and SynAN59. Each sample was run through an XBridge C18 reverse-phase column at 50 °C (see detail in Methods). The middle section framed

    Journal: Analytical biochemistry

    Article Title: Single-nucleotide resolution of RNAs up to 59 nucleotides by HPLC

    doi: 10.1016/j.ab.2012.12.011

    Figure Lengend Snippet: Single-nucleotide separation of RNAs up to 59 nt by HPLC. (A) Individual chromatogram for SynAN57, SynAN58 and SynAN59. Each sample was run through an XBridge C18 reverse-phase column at 50 °C (see detail in Methods). The middle section framed

    Article Snippet: For separating AN59-M1 from AN59-M2 on an XBridge C18 column, we further investigated the effect of temperature on elution.

    Techniques: High Performance Liquid Chromatography

    HPLC-DAD chromatograms of the crude extract and its polyamide fractions (PA1–PA5) of Combretum aff. laxum. SunFire C18 column (150 x 3 mm i.d., 3.5 μm); 5–100% MeCN/0.1% aqueous formic acid in 30min, 0.4 mL/min; detection: 210–700nm, maxplot.

    Journal: Scientia Pharmaceutica

    Article Title: Screening of Panamanian Plant Extracts for Pesticidal Properties and HPLC-Based Identification of Active Compounds

    doi: 10.3797/scipharm.1410-10

    Figure Lengend Snippet: HPLC-DAD chromatograms of the crude extract and its polyamide fractions (PA1–PA5) of Combretum aff. laxum. SunFire C18 column (150 x 3 mm i.d., 3.5 μm); 5–100% MeCN/0.1% aqueous formic acid in 30min, 0.4 mL/min; detection: 210–700nm, maxplot.

    Article Snippet: A SunFire C18 column (150 x 30 mm i.d., 5 μm; Waters) connected to a pre-column (10 x 30 mm) was used, at a flow rate of 20 mL/min.

    Techniques: High Performance Liquid Chromatography

    HPLC-DAD chromatograms of the crude extract and its polyamide fractions (PA1–PA5) of Erythroxylum macrophyllum. SunFire C18 column (150 x 3 mm i.d., 3.5 μm); 5–100% MeCN/0.1% aqueous formic acid in 30min, 0.4 mL/min; detection: 210–700nm, maxplot.

    Journal: Scientia Pharmaceutica

    Article Title: Screening of Panamanian Plant Extracts for Pesticidal Properties and HPLC-Based Identification of Active Compounds

    doi: 10.3797/scipharm.1410-10

    Figure Lengend Snippet: HPLC-DAD chromatograms of the crude extract and its polyamide fractions (PA1–PA5) of Erythroxylum macrophyllum. SunFire C18 column (150 x 3 mm i.d., 3.5 μm); 5–100% MeCN/0.1% aqueous formic acid in 30min, 0.4 mL/min; detection: 210–700nm, maxplot.

    Article Snippet: A SunFire C18 column (150 x 30 mm i.d., 5 μm; Waters) connected to a pre-column (10 x 30 mm) was used, at a flow rate of 20 mL/min.

    Techniques: High Performance Liquid Chromatography

    HPLC-DAD chromatograms of the crude extract and its polyamide fractions (PA1–PA5) of Myrcia splendens. SunFire C18 column (150 x 3 mm i.d., 3.5 μm); 5–100% MeCN/0.1% aqueous formic acid in 30min, 0.4 mL/min; detection: 210–700nm, maxplot.

    Journal: Scientia Pharmaceutica

    Article Title: Screening of Panamanian Plant Extracts for Pesticidal Properties and HPLC-Based Identification of Active Compounds

    doi: 10.3797/scipharm.1410-10

    Figure Lengend Snippet: HPLC-DAD chromatograms of the crude extract and its polyamide fractions (PA1–PA5) of Myrcia splendens. SunFire C18 column (150 x 3 mm i.d., 3.5 μm); 5–100% MeCN/0.1% aqueous formic acid in 30min, 0.4 mL/min; detection: 210–700nm, maxplot.

    Article Snippet: A SunFire C18 column (150 x 30 mm i.d., 5 μm; Waters) connected to a pre-column (10 x 30 mm) was used, at a flow rate of 20 mL/min.

    Techniques: High Performance Liquid Chromatography

    A total ion chromatogram of a standard mixture of UTL-5g (at m/z of 271.17 > 109.96), ISOX (at m/z of 128.05 > 109.96), DCA (at m/z of 161.92 > 125.95) and the internal standard zileuton (at m/z of 237.08 > 161.03) at the concentration of 1 μM. Chromatographic separation was performed on a Nova-Pak C18 column (4 μm, 3.9 mm × 150 mm) at 30°C, running with mobile phase consisting of 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B) at the flow rate of 0.2 mL/min. Mobile phase gradient B%(min) was programmed as 10 (0) → 95(3.5) → 95 (19) → 10(19.5) → 10(24). The retention times were 16.86 ± 0.02 for UTL-5g, 11.85 ± 0.01 for ISOX and 14.60 ± 0.02 for DCA, and 13.53 ± 0.02 for internal standard zileuton.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: A liquid chromatography with tandem mass spectrometry method for simultaneous determination of UTL-5g and its metabolites in human plasma

    doi: 10.1016/j.jchromb.2015.04.015

    Figure Lengend Snippet: A total ion chromatogram of a standard mixture of UTL-5g (at m/z of 271.17 > 109.96), ISOX (at m/z of 128.05 > 109.96), DCA (at m/z of 161.92 > 125.95) and the internal standard zileuton (at m/z of 237.08 > 161.03) at the concentration of 1 μM. Chromatographic separation was performed on a Nova-Pak C18 column (4 μm, 3.9 mm × 150 mm) at 30°C, running with mobile phase consisting of 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B) at the flow rate of 0.2 mL/min. Mobile phase gradient B%(min) was programmed as 10 (0) → 95(3.5) → 95 (19) → 10(19.5) → 10(24). The retention times were 16.86 ± 0.02 for UTL-5g, 11.85 ± 0.01 for ISOX and 14.60 ± 0.02 for DCA, and 13.53 ± 0.02 for internal standard zileuton.

    Article Snippet: UTL-5g, its metabolites DCA and ISOX, and the internal standard zileuton were separated from potentially interfering material on a Nova-Pak C18 column (4 μm, 3.9 mm × 150 mm; Waters Corp., Milford, MA) maintained at 30 °C.

    Techniques: Concentration Assay, Flow Cytometry

    Chromatograms of Medicago truncatula seed extract. A. Injection into a C18 column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.

    Journal: BMC Chemical Biology

    Article Title: High toxicity and specificity of the saponin 3-GlcA-28-AraRhaxyl-medicagenate, from Medicago truncatula seeds, for Sitophilus oryzae

    doi: 10.1186/1472-6769-12-3

    Figure Lengend Snippet: Chromatograms of Medicago truncatula seed extract. A. Injection into a C18 column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.

    Article Snippet: The extract was injected into a C18 column (250 x 4.1 mm, 3 μm, Phenomenex).

    Techniques: Injection, High Performance Liquid Chromatography, Flow Cytometry, Purification, Activity Assay

    High Performance Liquid Chromatography of the aqueous extract of J. mollissima. SP: Phenomenex Luna C18 column (250 × 4.6 mm, 5 μ m); MP: ACN gradient: acetic acid 0.3%; flow rate: 0.7 mL/min; detection: 340 nm. Four compounds were identified as isoorientin (peak 3), orientin (peak 4), vitexin (peak 5), and isovitexin (peak 6).

    Journal: BioMed Research International

    Article Title: Aqueous Leaf Extract of Jatropha mollissima (Pohl) Bail Decreases Local Effects Induced by Bothropic Venom

    doi: 10.1155/2016/6101742

    Figure Lengend Snippet: High Performance Liquid Chromatography of the aqueous extract of J. mollissima. SP: Phenomenex Luna C18 column (250 × 4.6 mm, 5 μ m); MP: ACN gradient: acetic acid 0.3%; flow rate: 0.7 mL/min; detection: 340 nm. Four compounds were identified as isoorientin (peak 3), orientin (peak 4), vitexin (peak 5), and isovitexin (peak 6).

    Article Snippet: The separation of compounds was performed at 25°C using a Phenomenex Luna C18 column (250 mm × 4.60 mm internal diameter, 5 μ m particle size).

    Techniques: High Performance Liquid Chromatography, Flow Cytometry

    The comparison of the separation of six ionophore coccidiostats with traditional porous column and fused core technology. (a) Chromatogram obtained on Phenomenex Luna C18(2) column (150 × 4.6 mm, 3 μ m) with gradient elution of methanol (A) and 0.02 M KH 2 PO 4 (B) 0–10 min: 88% A, 14–19 min: 90% A, 22–32 min: 88% A; flow rate 0.7 mL/min. (b) Chromatogram obtained on Phenomenex Kinetex C18 column (150 × 4.6 mm, 2.6 μ m) with isocratic elution with methanol and 0.02 M KH 2 PO 4 (88 : 12, v : v ); flow rate 0.7 mL/min.

    Journal: The Scientific World Journal

    Article Title: The Determination of Six Ionophore Coccidiostats in Feed by Liquid Chromatography with Postcolumn Derivatisation and Spectrofotometric/Fluorescence Detection

    doi: 10.1155/2013/763402

    Figure Lengend Snippet: The comparison of the separation of six ionophore coccidiostats with traditional porous column and fused core technology. (a) Chromatogram obtained on Phenomenex Luna C18(2) column (150 × 4.6 mm, 3 μ m) with gradient elution of methanol (A) and 0.02 M KH 2 PO 4 (B) 0–10 min: 88% A, 14–19 min: 90% A, 22–32 min: 88% A; flow rate 0.7 mL/min. (b) Chromatogram obtained on Phenomenex Kinetex C18 column (150 × 4.6 mm, 2.6 μ m) with isocratic elution with methanol and 0.02 M KH 2 PO 4 (88 : 12, v : v ); flow rate 0.7 mL/min.

    Article Snippet: Chromatographic separation of compounds was performed on Kinetex C18 column (150 × 4.6 mm, 2.6 μ m, Phenomenex, USA) connected with precolumn (4 × 3 mm, SecurityGuard, Phenomenex, USA).

    Techniques: Flow Cytometry

    Maternal VLDLR deletion elevates PAFs in pups and diminishes PAFAH in milk a–b, PAF levels were increased in the skin ( a ) and intestine ( b ) of the pups nursed by Vldlr −/− mothers compared to the pups nursed by WT control mothers. Tissue lipids were analyzed by MRM LC-MS/MS to quantify PAF-C16 (left) and PAF-C18 (right) levels (n=3). The results were normalized to d4-PAF-C16 or d4-PAF-C18 internal control, respectively. P, postnatal day. c, PAFAH activity was decreased in the milk of Vldlr −/− mothers compared to WT mothers (n=4). d, PAFAH activity was decreased in the plasma of the pups nursed by Vldlr −/− mothers compared to the pups nursed by WT mothers (n=8). For a, b, d , the pups nursed by Vldlr −/− mothers were Vldlr+/−, and the pups nursed by WT mothers were WT. e, PAFAH activity was decreased in the culture medium of Vldlr −/− macrophages compared to WT macrophages that were differentiated from bone marrow (BM) or splenocyte (Sp) (n=4). For e , 1×10 6 macrophages were differentiated from 1.5×10 6 bone marrow cells or 5×10 6 splenocytes of Vldlr −/− mice or WT control mice (male, 3 month old). PAFAH activity was normalized to macrophage cell number (×10 6 ). Statistical analyses were performed with Student’s t-Test and all data are shown as mean ± standard deviation; **, p

    Journal: Nature communications

    Article Title: Macrophage VLDL Receptor Promotes PAFAH Secretion in Mother's Milk and Suppresses Systemic Inflammation in Nursing Neonates

    doi: 10.1038/ncomms2011

    Figure Lengend Snippet: Maternal VLDLR deletion elevates PAFs in pups and diminishes PAFAH in milk a–b, PAF levels were increased in the skin ( a ) and intestine ( b ) of the pups nursed by Vldlr −/− mothers compared to the pups nursed by WT control mothers. Tissue lipids were analyzed by MRM LC-MS/MS to quantify PAF-C16 (left) and PAF-C18 (right) levels (n=3). The results were normalized to d4-PAF-C16 or d4-PAF-C18 internal control, respectively. P, postnatal day. c, PAFAH activity was decreased in the milk of Vldlr −/− mothers compared to WT mothers (n=4). d, PAFAH activity was decreased in the plasma of the pups nursed by Vldlr −/− mothers compared to the pups nursed by WT mothers (n=8). For a, b, d , the pups nursed by Vldlr −/− mothers were Vldlr+/−, and the pups nursed by WT mothers were WT. e, PAFAH activity was decreased in the culture medium of Vldlr −/− macrophages compared to WT macrophages that were differentiated from bone marrow (BM) or splenocyte (Sp) (n=4). For e , 1×10 6 macrophages were differentiated from 1.5×10 6 bone marrow cells or 5×10 6 splenocytes of Vldlr −/− mice or WT control mice (male, 3 month old). PAFAH activity was normalized to macrophage cell number (×10 6 ). Statistical analyses were performed with Student’s t-Test and all data are shown as mean ± standard deviation; **, p

    Article Snippet: A Gemini C18 column (Phenomenex) was used for a reverse phase separation of the lipids, with mobile phase A as 0.1% formic acid v/v, and mobile phase B as 100% acetonitrile, 0.1% formic acid v/v.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Activity Assay, Mouse Assay, Standard Deviation

    Oral PAFAH supplementation to the pups rescues the neonatal toxicity Pups from a Vldlr −/− mother were orally gavaged with recombinant PAFAH at 0.15 mg/kg/day starting P1 (n=3); as internal controls, pups in the same litter were gavaged with vehicle PBS (n=3). The pups were analyzed at P21. a, A representative image showing the rescue of pup hair loss by PAFAH. b, Immuno-fluorescence staining showing the reduction of leukocytes (CD11b + ) in the pup skin by PAFAH. Scale bars, 100μm. c, RT-QPCR analysis showing the attenuated expression of inflammatory genes in the pup skin by PAFAH (n=3). d, LC-MS/MS analysis showing the reduced levels of PAF-C16 (left) and PAF-C18 (right) in the intestine and skin of the PAFAH-treated pups (n=3). e, Western blot analyses of PAFAH protein. Left: comparison of two anti-PAFAH antibodies. Representative result of two independent experiments is shown. The abbreviations are: CM, conditioned medium; m, mouse; h, human; r, recombinant; BMM, bone marrow macrophage. Middle: western blot image for the detection of endogenous (top) and total (bottom) PAFAH in the serum of pups gavaged with PAFAH or PBS control. Right: quantification of the ratio of total/endogenous PAFAH protein in pup serum (n=2). Statistical analyses were performed with Student’s t-Test and all data are shown as mean ± standard deviation; *, p

    Journal: Nature communications

    Article Title: Macrophage VLDL Receptor Promotes PAFAH Secretion in Mother's Milk and Suppresses Systemic Inflammation in Nursing Neonates

    doi: 10.1038/ncomms2011

    Figure Lengend Snippet: Oral PAFAH supplementation to the pups rescues the neonatal toxicity Pups from a Vldlr −/− mother were orally gavaged with recombinant PAFAH at 0.15 mg/kg/day starting P1 (n=3); as internal controls, pups in the same litter were gavaged with vehicle PBS (n=3). The pups were analyzed at P21. a, A representative image showing the rescue of pup hair loss by PAFAH. b, Immuno-fluorescence staining showing the reduction of leukocytes (CD11b + ) in the pup skin by PAFAH. Scale bars, 100μm. c, RT-QPCR analysis showing the attenuated expression of inflammatory genes in the pup skin by PAFAH (n=3). d, LC-MS/MS analysis showing the reduced levels of PAF-C16 (left) and PAF-C18 (right) in the intestine and skin of the PAFAH-treated pups (n=3). e, Western blot analyses of PAFAH protein. Left: comparison of two anti-PAFAH antibodies. Representative result of two independent experiments is shown. The abbreviations are: CM, conditioned medium; m, mouse; h, human; r, recombinant; BMM, bone marrow macrophage. Middle: western blot image for the detection of endogenous (top) and total (bottom) PAFAH in the serum of pups gavaged with PAFAH or PBS control. Right: quantification of the ratio of total/endogenous PAFAH protein in pup serum (n=2). Statistical analyses were performed with Student’s t-Test and all data are shown as mean ± standard deviation; *, p

    Article Snippet: A Gemini C18 column (Phenomenex) was used for a reverse phase separation of the lipids, with mobile phase A as 0.1% formic acid v/v, and mobile phase B as 100% acetonitrile, 0.1% formic acid v/v.

    Techniques: Recombinant, Fluorescence, Staining, Quantitative RT-PCR, Expressing, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Western Blot, Standard Deviation

    Reverse phase HPLC of trypsin-digested human butyrylcholinesterase labeled with thiomethyl sarin Rp . The 0.18 mg of labeled human butyrylcholinesterase (2.2 nanomoles) was digested with trypsin and loaded onto a 100 × 4.6 mm Phenomenex C18 column.

    Journal: Chemical research in toxicology

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase

    doi: 10.1021/tx900090m

    Figure Lengend Snippet: Reverse phase HPLC of trypsin-digested human butyrylcholinesterase labeled with thiomethyl sarin Rp . The 0.18 mg of labeled human butyrylcholinesterase (2.2 nanomoles) was digested with trypsin and loaded onto a 100 × 4.6 mm Phenomenex C18 column.

    Article Snippet: Digests were centrifuged to remove a pellet and injected into a Phenomenex C18 column, 100 × 4.6 mm, on a Waters 625 LC system.

    Techniques: High Performance Liquid Chromatography, Labeling

    HPLC-FLD chromatograms after periodate oxidation from: a) overlaid toxins standards of dcNEO (0.37 μM) and dcSTX (0.70 μM), both producing two oxidation products coded ‘1’ and ‘2’; b) urine sample from patient A on 2018-10-11 after C18 cleanup dominated by dcSTX oxidation products.

    Journal: Toxicon: X

    Article Title: Paralytic shellfish poisoning due to ingestion of contaminated mussels: A 2018 case report in Caparica (Portugal)

    doi: 10.1016/j.toxcx.2019.100017

    Figure Lengend Snippet: HPLC-FLD chromatograms after periodate oxidation from: a) overlaid toxins standards of dcNEO (0.37 μM) and dcSTX (0.70 μM), both producing two oxidation products coded ‘1’ and ‘2’; b) urine sample from patient A on 2018-10-11 after C18 cleanup dominated by dcSTX oxidation products.

    Article Snippet: Separation was performed on a Supelcosil LC-18, 150 × 4.6 mm, 5 μm column (Supelco, USA) equipped with a C18 guard column (SecurityGuard™, 4 × 3 mm, Luna, Phenomenex, USA).

    Techniques: High Performance Liquid Chromatography

    Chromatograms of representative semi-preparative HPLC purification of [ 18 F]VAT. Agilent Zorbax SB-C18 column, 38% acetonitrile in 62% 0.1 M ammonium formate, v/v, pH 6.5, 4 mL/min. Panel A: radiochemical trace and panel B: UV trace at 254 nm.

    Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine

    Article Title: Automated production of [18F]VAT suitable for clinical PET study of vesicular acetylcholine transporter

    doi: 10.1016/j.apradiso.2015.09.010

    Figure Lengend Snippet: Chromatograms of representative semi-preparative HPLC purification of [ 18 F]VAT. Agilent Zorbax SB-C18 column, 38% acetonitrile in 62% 0.1 M ammonium formate, v/v, pH 6.5, 4 mL/min. Panel A: radiochemical trace and panel B: UV trace at 254 nm.

    Article Snippet: An Agilent Zorbax SB-C18 column (5 μ m, 4.6 × 250 mm) equipped with a Grace Alltima C18 guard column (5 μ m, 7.5 × 4.6 mm) was used for quality control.

    Techniques: High Performance Liquid Chromatography, Purification

    Chromatograms of representative semi-preparative HPLC purification of 2-[ 18 F]fluoroethyl tosylate. Agilent Zorbax SB-C18 column, 50% acetonitrile in 50% 0.1 M ammonium formate, v/v, pH 6.5, 5 mL/min. Panel A: radiochemical trace and panel B: UV trace

    Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine

    Article Title: Automated production of [18F]VAT suitable for clinical PET study of vesicular acetylcholine transporter

    doi: 10.1016/j.apradiso.2015.09.010

    Figure Lengend Snippet: Chromatograms of representative semi-preparative HPLC purification of 2-[ 18 F]fluoroethyl tosylate. Agilent Zorbax SB-C18 column, 50% acetonitrile in 50% 0.1 M ammonium formate, v/v, pH 6.5, 5 mL/min. Panel A: radiochemical trace and panel B: UV trace

    Article Snippet: An Agilent Zorbax SB-C18 column (5 μ m, 4.6 × 250 mm) equipped with a Grace Alltima C18 guard column (5 μ m, 7.5 × 4.6 mm) was used for quality control.

    Techniques: High Performance Liquid Chromatography, Purification

    Optimized chromatogram of flupirtine maleate (10.3 min) on a C18 column

    Journal: Scientia Pharmaceutica

    Article Title: Identification of Degradation Products and a Stability-Indicating RP-HPLC Method for the Determination of Flupirtine Maleate in Pharmaceutical Dosage Forms

    doi: 10.3797/scipharm.1310-01

    Figure Lengend Snippet: Optimized chromatogram of flupirtine maleate (10.3 min) on a C18 column

    Article Snippet: Method Development and Optimization of the Chromatographic Conditions In preliminary experiments, the drug was subjected to the reversed-phase mode using a C18 column (Agilent, 250 × 4.6 mm, 5μ) and mobile phases consisting of water (pH 3.0 adjusted with orthophosphoric acid) and methanol by varying the % aqueous phase from 10% to 30%.

    Techniques:

    HPLC chromatogram of F. distichus and its separation into eight fractions using Zorbax Eclipse XDB-C18 column (4.6 mm × 50 mm, 1.8 µm). The collected fractions: F1 = 0.4–0.5 min; F7 = 5.4–5.5 min.

    Journal: Marine Drugs

    Article Title: The Identification of a SIRT6 Activator from Brown Algae Fucus distichus

    doi: 10.3390/md15060190

    Figure Lengend Snippet: HPLC chromatogram of F. distichus and its separation into eight fractions using Zorbax Eclipse XDB-C18 column (4.6 mm × 50 mm, 1.8 µm). The collected fractions: F1 = 0.4–0.5 min; F7 = 5.4–5.5 min.

    Article Snippet: HPLC Analysis The chromatographic separation of H3K9 and acetylated H3K9 was achieved on a Zorbax Eclipse XDB-C18 column (4.6 mm × 50 mm, 1.8 µm; Agilent Technologies, Santa Clara, CA, USA) at room temperature using a Shimadzu prominence system (Shimadzu Technology, Kyoto, Japan) consisting of a CBM-20A, LC-20 AB binary pumps, an SIL-20AC-HT auto-sampler, and a DGU-20A3 degassing unit.

    Techniques: High Performance Liquid Chromatography

    HPLC analysis of a) [ 18 F]NST732 after formulation; b) [ 18 F]NST732 co-injected with the non-radioactive standard. HPLC conditions: Agilent Eclipse XDB C18 4.6 × 150 mm, 5 μm column, 20–40% acetonitrile in water (0.1% TFA) for 10

    Journal: Nuclear Medicine and Biology

    Article Title: Synthesis of ApoSense compound [18F]2-(5-(dimethylamino)naphthalene-1-sulfonamido)-2-(fluoromethyl)butanoic acid ([18F]NST732) by nucleophilic ring-opening of an aziridine precursor

    doi: 10.1016/j.nucmedbio.2011.12.008

    Figure Lengend Snippet: HPLC analysis of a) [ 18 F]NST732 after formulation; b) [ 18 F]NST732 co-injected with the non-radioactive standard. HPLC conditions: Agilent Eclipse XDB C18 4.6 × 150 mm, 5 μm column, 20–40% acetonitrile in water (0.1% TFA) for 10

    Article Snippet: Analytical HPLC analyses for radiochemical work were performed on an Agilent 1200 Series instrument equipped with multi-wavelength detectors using an Agilent Eclipse XDB C18 column (4.6 × 150 mm, 5 μm) with a flow rate of 1.0 mL/min.

    Techniques: High Performance Liquid Chromatography, Injection

    HPLC analysis of the crude reaction mixture of compound 5 (t R = 7.3 min) and its regioisomer ( 6 , t R = 8.7 min). HPLC conditions: Agilent Eclipse XDB C18 5 μm, 4.6 × 150 mm column, 35–50% acetonitrile in water (0.1% TFA) in 10 min,

    Journal: Nuclear Medicine and Biology

    Article Title: Synthesis of ApoSense compound [18F]2-(5-(dimethylamino)naphthalene-1-sulfonamido)-2-(fluoromethyl)butanoic acid ([18F]NST732) by nucleophilic ring-opening of an aziridine precursor

    doi: 10.1016/j.nucmedbio.2011.12.008

    Figure Lengend Snippet: HPLC analysis of the crude reaction mixture of compound 5 (t R = 7.3 min) and its regioisomer ( 6 , t R = 8.7 min). HPLC conditions: Agilent Eclipse XDB C18 5 μm, 4.6 × 150 mm column, 35–50% acetonitrile in water (0.1% TFA) in 10 min,

    Article Snippet: Analytical HPLC analyses for radiochemical work were performed on an Agilent 1200 Series instrument equipped with multi-wavelength detectors using an Agilent Eclipse XDB C18 column (4.6 × 150 mm, 5 μm) with a flow rate of 1.0 mL/min.

    Techniques: High Performance Liquid Chromatography

    HPLC analysis of the crude reaction mixture of [ 18 F]NST732 (t R = 7.4 min) and its regioisomer ( 7 , t R = 6.0 min). HPLC conditions: Agilent Eclipse XDB C18 4.6 × 150 mm, 5 μm column, 20–40% acetonitrile in water (0.1% TFA) for 10

    Journal: Nuclear Medicine and Biology

    Article Title: Synthesis of ApoSense compound [18F]2-(5-(dimethylamino)naphthalene-1-sulfonamido)-2-(fluoromethyl)butanoic acid ([18F]NST732) by nucleophilic ring-opening of an aziridine precursor

    doi: 10.1016/j.nucmedbio.2011.12.008

    Figure Lengend Snippet: HPLC analysis of the crude reaction mixture of [ 18 F]NST732 (t R = 7.4 min) and its regioisomer ( 7 , t R = 6.0 min). HPLC conditions: Agilent Eclipse XDB C18 4.6 × 150 mm, 5 μm column, 20–40% acetonitrile in water (0.1% TFA) for 10

    Article Snippet: Analytical HPLC analyses for radiochemical work were performed on an Agilent 1200 Series instrument equipped with multi-wavelength detectors using an Agilent Eclipse XDB C18 column (4.6 × 150 mm, 5 μm) with a flow rate of 1.0 mL/min.

    Techniques: High Performance Liquid Chromatography

    Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus C18 column connected to a Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus C18 column connected to a Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.

    Article Snippet: Chromatography for LC-MS was performed using a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology, Santa Clara, CA, USA), an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), an Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters, Milford, MA, USA) or a Pursuit 200Å PFP column (4.6 × 150 mm, 5 µm) (Agilent Technology, Santa Clara, CA, USA).

    Techniques:

    Detection of 1,25(OH) 2 D3 in honey. ( A ) Whole extract was analyzed directly using LC-MS with a Zorbax Eclipse Plus C18 column with a methanol gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + . ( B ) The sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of 1,25(OH) 2 D3 in honey. ( A ) Whole extract was analyzed directly using LC-MS with a Zorbax Eclipse Plus C18 column with a methanol gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + . ( B ) The sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

    Article Snippet: Chromatography for LC-MS was performed using a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology, Santa Clara, CA, USA), an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), an Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters, Milford, MA, USA) or a Pursuit 200Å PFP column (4.6 × 150 mm, 5 µm) (Agilent Technology, Santa Clara, CA, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Purification

    Detection of 25(OH)D3 in honey. After a pre-purification step using a C18 column with an acetonitrile gradient, the fraction was analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 383.331 [M + H − H 2 O] + .

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of 25(OH)D3 in honey. After a pre-purification step using a C18 column with an acetonitrile gradient, the fraction was analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 383.331 [M + H − H 2 O] + .

    Article Snippet: Chromatography for LC-MS was performed using a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology, Santa Clara, CA, USA), an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), an Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters, Milford, MA, USA) or a Pursuit 200Å PFP column (4.6 × 150 mm, 5 µm) (Agilent Technology, Santa Clara, CA, USA).

    Techniques: Purification, Liquid Chromatography with Mass Spectroscopy

    Detection of 1,20(OH) 2 D3 in honey. ( A ) The peak corresponding in RT to standard was collected from commercially available honey using an Atlantis C18 column using a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 439.319 [M + Na] + . ( B ) The local honey sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of 1,20(OH) 2 D3 in honey. ( A ) The peak corresponding in RT to standard was collected from commercially available honey using an Atlantis C18 column using a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 439.319 [M + Na] + . ( B ) The local honey sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

    Article Snippet: Chromatography for LC-MS was performed using a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology, Santa Clara, CA, USA), an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), an Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters, Milford, MA, USA) or a Pursuit 200Å PFP column (4.6 × 150 mm, 5 µm) (Agilent Technology, Santa Clara, CA, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Purification

    HPLC analysis of a) Sep-Pak purified [ 18 F]c(RGDfK); b) HPLC purified [ 18 F]c(RGDfK); c) [ 18 F]c(RGDfK), co-injected with the non-radioactive standard. HPLC condition: Agilent Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm), mobile phase: 10 - 50% A in 8 min, 50 – 90 % A in 15 min. A = acetonitrile (0.1% TFA), B = water (0.1% TFA), with a flow rate of 1.0 mL/min. Solid line, in-line radiodetector; dotted line, UV detector at 254 nm.

    Journal: Journal of labelled compounds & radiopharmaceuticals

    Article Title: Fast Indirect Fluorine-18 Labeling of Protein/Peptide using the useful 6-Fluoronicotinic acid-2,3,5,6-Tetrafluorophenyl prosthetic group: A Method Comparable to direct Fluorination

    doi: 10.1002/jlcr.3487

    Figure Lengend Snippet: HPLC analysis of a) Sep-Pak purified [ 18 F]c(RGDfK); b) HPLC purified [ 18 F]c(RGDfK); c) [ 18 F]c(RGDfK), co-injected with the non-radioactive standard. HPLC condition: Agilent Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm), mobile phase: 10 - 50% A in 8 min, 50 – 90 % A in 15 min. A = acetonitrile (0.1% TFA), B = water (0.1% TFA), with a flow rate of 1.0 mL/min. Solid line, in-line radiodetector; dotted line, UV detector at 254 nm.

    Article Snippet: HPLC condition: Agilent Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm).

    Techniques: High Performance Liquid Chromatography, Purification, Injection, Flow Cytometry

    Identification of heme O by LC-MS/MS in schizonts extracts. Heme O was separated using C18 Vac columns and eluted with DMSO and analyzed by LC-MS / MS. ( A ) Heme O separation was performed using a Hypersil Gold C18 Column (see material and methods: Mass Spectrometry; LC-MS/MS) and the peak with retention time of 11.58 was analyzed by MS/MS. ( B) MS/MS of peak of 11.58 showing the m/z of heme O (arrow, 839.4, compatible with its calculated mass).

    Journal: Scientific Reports

    Article Title: Biosynthesis of heme O in intraerythrocytic stages of Plasmodium falciparum and potential inhibitors of this pathway

    doi: 10.1038/s41598-019-55506-y

    Figure Lengend Snippet: Identification of heme O by LC-MS/MS in schizonts extracts. Heme O was separated using C18 Vac columns and eluted with DMSO and analyzed by LC-MS / MS. ( A ) Heme O separation was performed using a Hypersil Gold C18 Column (see material and methods: Mass Spectrometry; LC-MS/MS) and the peak with retention time of 11.58 was analyzed by MS/MS. ( B) MS/MS of peak of 11.58 showing the m/z of heme O (arrow, 839.4, compatible with its calculated mass).

    Article Snippet: The separation was performed using a Hypersil Gold C18 Column (100 × 2.1 mm, i.d., 5 μm; Thermo Scientific, Bremen, Germany).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    The R2 fraction prepared from the ethyl acetate fraction of the roasted coffee bean extract exhibits anti-inflammatory activity. ( A ) Procedure for the fractionation of the ethyl acetate fraction of coffee bean extract using the Sep Pak C18 column. ( B ) The ethyl acetate fraction of the roasted coffee bean extract ( E ) and the R1-R3 fractions were analyzed by HPLC. ( C – F ) RAW264.7 cells were pretreated with fractions R1-R3, equivalent to 5% (v/v) of coffee, for 1 h prior to the LPS stimulation (1 μg/mL). ( C ) Nitrate concentrations in culture supernatants were measured 24 h after the LPS stimulation using Griess reagent. ( D ) iNOS mRNA expression was assessed 12 h after the LPS stimulation by RT-PCR. GAPDH mRNA expression was used as an internal control. ( E ) The amounts of CCL2 in supernatants were evaluated 24 h after the LPS stimulation by ELISA. ( F ) CCL2 mRNA expression was assessed 2 h after the LPS stimulation by RT-PCR. ( G ) RAW264.7 cells were transfected with pNF-κB-Luc and pRL-TK. Transfected cells were pretreated with fractions R1-R3, equivalent to 5% (v/v) of coffee, for 1 h prior to the stimulation with LPS (1 μg/mL) for 6 h. NF-κB-dependent luciferase activity was normalized to the activity of constitutively expressed Renilla luciferase. ( H ) RAW264.7 cells were treated with fractions R1-R3, equivalent to 5% (v/v) of coffee, for 1 h. Nuclear extracts were immunoblotted with an anti-Nrf2 or anti-Lamin B antibody. The relative expression levels of Nrf2 in the nucleus are shown in the graph.

    Journal: Scientific Reports

    Article Title: Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2

    doi: 10.1038/s41598-020-59380-x

    Figure Lengend Snippet: The R2 fraction prepared from the ethyl acetate fraction of the roasted coffee bean extract exhibits anti-inflammatory activity. ( A ) Procedure for the fractionation of the ethyl acetate fraction of coffee bean extract using the Sep Pak C18 column. ( B ) The ethyl acetate fraction of the roasted coffee bean extract ( E ) and the R1-R3 fractions were analyzed by HPLC. ( C – F ) RAW264.7 cells were pretreated with fractions R1-R3, equivalent to 5% (v/v) of coffee, for 1 h prior to the LPS stimulation (1 μg/mL). ( C ) Nitrate concentrations in culture supernatants were measured 24 h after the LPS stimulation using Griess reagent. ( D ) iNOS mRNA expression was assessed 12 h after the LPS stimulation by RT-PCR. GAPDH mRNA expression was used as an internal control. ( E ) The amounts of CCL2 in supernatants were evaluated 24 h after the LPS stimulation by ELISA. ( F ) CCL2 mRNA expression was assessed 2 h after the LPS stimulation by RT-PCR. ( G ) RAW264.7 cells were transfected with pNF-κB-Luc and pRL-TK. Transfected cells were pretreated with fractions R1-R3, equivalent to 5% (v/v) of coffee, for 1 h prior to the stimulation with LPS (1 μg/mL) for 6 h. NF-κB-dependent luciferase activity was normalized to the activity of constitutively expressed Renilla luciferase. ( H ) RAW264.7 cells were treated with fractions R1-R3, equivalent to 5% (v/v) of coffee, for 1 h. Nuclear extracts were immunoblotted with an anti-Nrf2 or anti-Lamin B antibody. The relative expression levels of Nrf2 in the nucleus are shown in the graph.

    Article Snippet: To separate ethyl acetate extracts, dried ethyl acetate extracts (equivalent to 15 mL coffee) were dissolved in 15 mL of methanol/ethyl acetate (2:1), applied to a Sep-Pak C18 column (Waters, Milford, MA), and eluted by 15-mL stepwise rinses of water (R1), 20% methanol (R2), and 100% methanol (R3).

    Techniques: Activity Assay, Fractionation, High Performance Liquid Chromatography, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Transfection, Luciferase