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MedChemExpress
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Tocris
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Addgene inc
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Santa Cruz Biotechnology
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Croda International Plc
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Croda International Plc
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Danaher Inc
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Croda International Plc
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Danaher Inc
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Tocris
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Croda International Plc
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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: Elucidating the mechanism of triphenyl phosphate interference in bone metabolism via network toxicology and molecular docking methodologies
doi: 10.3389/fendo.2025.1606877
Figure Lengend Snippet: Effects of TPHP on osteoblast function. (A, B) TPhP inhibits cell migration (Wound healing assay). (C) TPhP reduces cell proliferation (CCK-8 assay). (D, E) TPhP suppresses migration and invasion (Transwell assay). (F) Western blot showing TPhP effects on MAPK and EMT proteins. (G, H) MEK/ERK activator (C16-PAF) rescues TPhP-inhibited migration/invasion (G) and proliferation (H) , while an inhibitor (MEK-IN-6) does not. (I) Western blot confirming MAPK/ERK modulation in rescue experiments. (J) qPCR showing TPhP downregulates key target genes.(*P < 0.05, **P < 0.01, ***P < 0.001).
Article Snippet: For pathway validation experiments, cells were treated with TPhP (40 μM) alone or in combination with the
Techniques: Migration, Wound Healing Assay, CCK-8 Assay, Transwell Assay, Western Blot
Journal: Genes
Article Title: Inhibitory Effect of Interleukin-24 on Programmed Death Ligand 1 Expression via a Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2-Dependent Pathway in Human Triple-Negative Breast Cancer
doi: 10.3390/genes17030339
Figure Lengend Snippet: IL-24 reduces PD-L1 expression by activating PKR . MDA-MB-231 cancer cells were treated with either a non-replicative virus (MOI 180) encoding IL-24–expressing constructs or a control (Ad.vector) construct, C16 (a PKR inhibitor), or both. ( A ) PD-L1 protein levels were measured by Western blot after treatment with or without IL-24 and C16. The figure shown is representative of four independent experiments. ( B ) Western blot quantification of PD-L1 relative to β-actin was performed using Odyssey Imaging System software v.5.2. Comparisons between groups were done with two-way ANOVA, followed by Tukey’s post hoc test. *** p < 0.001, **** p < 0.0001, ns: not significant.
Article Snippet:
Techniques: Expressing, Virus, Construct, Control, Plasmid Preparation, Western Blot, Imaging, Software