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  • 96
    Thermo Fisher c12
    Nutrient transport and brush border membrane expression. 58 Fe transport (A and B) or uptake (E, F), 67 Zn transport (C and D) or uptake (G, H), fluorescent BODIPY® 500/510 C1, <t>C12</t> lipid uptake (I and J), and alkaline phosphatase activity (K and L) in response to acute (A, C, E, G, I, K) and chronic (B, D, F, H, J, L) doses of TiO 2 . Data is mean±SEM. * denotes significance according to an unpaired Student’s t-test, p
    C12, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore c12
    Transduction efficiency. Flow cytometry analysis of <t>C12</t> cells infected with equal vector genome amounts of transfection or infection made recombinant adeno-associated virus (rAAV)9-GFP. Purified vectors preparations obtained by transfection ( n = 4) or infection ( n = 5) were pooled and used to infected cells at various multiplicity of infections and GFP expression detected by FACS10. Average from duplicates with standard deviation are depicted.
    C12, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Avanti Polar c12 ceramide
    THC, but not nutrient deprivation, decreases the <t>ceramide:dihydroceramide</t> ratio in an autophagosome-enriched fraction. (A) Characterization of the presence of the autophagy marker MAP1LC3B-II and the lysosomal marker LAMP1 in fractions obtained from U87MG cells incubated for 6 h with EBSS or THC (6 µM) and subjected to subcellular fractionation in an OptiPrep® gradient. Note that MAP1LC3B-II appears in fractions of higher density in samples derived from THC-treated cells than in those derived from cells incubated with EBSS, (n = 2). (B) Analysis of the molecular species of ceramides and dihydroceramides present in the MAP1LC3B-II-enriched fraction (derived from cells treated with THC or incubated with EBSS) shown in (A). Data correspond to the ceramide:dihydroceramide ratio (upper panel) and the amount of each sphingolipid species (lower panel) in one representative experiment (n = 2). (C) Generation of ceramide rigid domains in C16 dihydroceramide-containing GUVs. Upper panel: Rigid, dihydroceramide-enriched domains (flower-like dark areas) in bilayers containing 80 mol % sn -1-palmitoyl-2-oleoyl phosphatidylcholine (POPC, a fluid phospholipid) and 20 mol % C16 dihydroceramide. Lower panel: a control experiment with a <t>C12</t> dihydroceramide that does not give rise to domains under these conditions. Bars: 10 µm. (D) Release of vesicular aqueous contents induced by ceramides. Effect of the different proportions of C16 ceramide:C16 dihydroceramide generated by the action of sphingomyelinase in LUVs composed of the following: dhSM:PC:Ch (30:67:3; red line); SM:dhSM:PC:Ch (20:10:67:3; magenta line); SM:PC:Ch (30:67:3; blue line); and SM:dhSM:PC:Ch (26:4:67:3; green line). A representative example of 3 closely similar experiments is shown. SM, sphingomyelin; dhSM, dihydrosphingomyelin; PC, phosphatidylcholine; Ch, cholesterol.
    C12 Ceramide, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    InvivoGen c12 iedap c12
    THC, but not nutrient deprivation, decreases the <t>ceramide:dihydroceramide</t> ratio in an autophagosome-enriched fraction. (A) Characterization of the presence of the autophagy marker MAP1LC3B-II and the lysosomal marker LAMP1 in fractions obtained from U87MG cells incubated for 6 h with EBSS or THC (6 µM) and subjected to subcellular fractionation in an OptiPrep® gradient. Note that MAP1LC3B-II appears in fractions of higher density in samples derived from THC-treated cells than in those derived from cells incubated with EBSS, (n = 2). (B) Analysis of the molecular species of ceramides and dihydroceramides present in the MAP1LC3B-II-enriched fraction (derived from cells treated with THC or incubated with EBSS) shown in (A). Data correspond to the ceramide:dihydroceramide ratio (upper panel) and the amount of each sphingolipid species (lower panel) in one representative experiment (n = 2). (C) Generation of ceramide rigid domains in C16 dihydroceramide-containing GUVs. Upper panel: Rigid, dihydroceramide-enriched domains (flower-like dark areas) in bilayers containing 80 mol % sn -1-palmitoyl-2-oleoyl phosphatidylcholine (POPC, a fluid phospholipid) and 20 mol % C16 dihydroceramide. Lower panel: a control experiment with a <t>C12</t> dihydroceramide that does not give rise to domains under these conditions. Bars: 10 µm. (D) Release of vesicular aqueous contents induced by ceramides. Effect of the different proportions of C16 ceramide:C16 dihydroceramide generated by the action of sphingomyelinase in LUVs composed of the following: dhSM:PC:Ch (30:67:3; red line); SM:dhSM:PC:Ch (20:10:67:3; magenta line); SM:PC:Ch (30:67:3; blue line); and SM:dhSM:PC:Ch (26:4:67:3; green line). A representative example of 3 closely similar experiments is shown. SM, sphingomyelin; dhSM, dihydrosphingomyelin; PC, phosphatidylcholine; Ch, cholesterol.
    C12 Iedap C12, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Avanti Polar nbd c12 ceramide
    THC, but not nutrient deprivation, decreases the <t>ceramide:dihydroceramide</t> ratio in an autophagosome-enriched fraction. (A) Characterization of the presence of the autophagy marker MAP1LC3B-II and the lysosomal marker LAMP1 in fractions obtained from U87MG cells incubated for 6 h with EBSS or THC (6 µM) and subjected to subcellular fractionation in an OptiPrep® gradient. Note that MAP1LC3B-II appears in fractions of higher density in samples derived from THC-treated cells than in those derived from cells incubated with EBSS, (n = 2). (B) Analysis of the molecular species of ceramides and dihydroceramides present in the MAP1LC3B-II-enriched fraction (derived from cells treated with THC or incubated with EBSS) shown in (A). Data correspond to the ceramide:dihydroceramide ratio (upper panel) and the amount of each sphingolipid species (lower panel) in one representative experiment (n = 2). (C) Generation of ceramide rigid domains in C16 dihydroceramide-containing GUVs. Upper panel: Rigid, dihydroceramide-enriched domains (flower-like dark areas) in bilayers containing 80 mol % sn -1-palmitoyl-2-oleoyl phosphatidylcholine (POPC, a fluid phospholipid) and 20 mol % C16 dihydroceramide. Lower panel: a control experiment with a <t>C12</t> dihydroceramide that does not give rise to domains under these conditions. Bars: 10 µm. (D) Release of vesicular aqueous contents induced by ceramides. Effect of the different proportions of C16 ceramide:C16 dihydroceramide generated by the action of sphingomyelinase in LUVs composed of the following: dhSM:PC:Ch (30:67:3; red line); SM:dhSM:PC:Ch (20:10:67:3; magenta line); SM:PC:Ch (30:67:3; blue line); and SM:dhSM:PC:Ch (26:4:67:3; green line). A representative example of 3 closely similar experiments is shown. SM, sphingomyelin; dhSM, dihydrosphingomyelin; PC, phosphatidylcholine; Ch, cholesterol.
    Nbd C12 Ceramide, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Avanti Polar lauroyl c12 l carnitine
    THC, but not nutrient deprivation, decreases the <t>ceramide:dihydroceramide</t> ratio in an autophagosome-enriched fraction. (A) Characterization of the presence of the autophagy marker MAP1LC3B-II and the lysosomal marker LAMP1 in fractions obtained from U87MG cells incubated for 6 h with EBSS or THC (6 µM) and subjected to subcellular fractionation in an OptiPrep® gradient. Note that MAP1LC3B-II appears in fractions of higher density in samples derived from THC-treated cells than in those derived from cells incubated with EBSS, (n = 2). (B) Analysis of the molecular species of ceramides and dihydroceramides present in the MAP1LC3B-II-enriched fraction (derived from cells treated with THC or incubated with EBSS) shown in (A). Data correspond to the ceramide:dihydroceramide ratio (upper panel) and the amount of each sphingolipid species (lower panel) in one representative experiment (n = 2). (C) Generation of ceramide rigid domains in C16 dihydroceramide-containing GUVs. Upper panel: Rigid, dihydroceramide-enriched domains (flower-like dark areas) in bilayers containing 80 mol % sn -1-palmitoyl-2-oleoyl phosphatidylcholine (POPC, a fluid phospholipid) and 20 mol % C16 dihydroceramide. Lower panel: a control experiment with a <t>C12</t> dihydroceramide that does not give rise to domains under these conditions. Bars: 10 µm. (D) Release of vesicular aqueous contents induced by ceramides. Effect of the different proportions of C16 ceramide:C16 dihydroceramide generated by the action of sphingomyelinase in LUVs composed of the following: dhSM:PC:Ch (30:67:3; red line); SM:dhSM:PC:Ch (20:10:67:3; magenta line); SM:PC:Ch (30:67:3; blue line); and SM:dhSM:PC:Ch (26:4:67:3; green line). A representative example of 3 closely similar experiments is shown. SM, sphingomyelin; dhSM, dihydrosphingomyelin; PC, phosphatidylcholine; Ch, cholesterol.
    Lauroyl C12 L Carnitine, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Phenomenex c12
    THC, but not nutrient deprivation, decreases the <t>ceramide:dihydroceramide</t> ratio in an autophagosome-enriched fraction. (A) Characterization of the presence of the autophagy marker MAP1LC3B-II and the lysosomal marker LAMP1 in fractions obtained from U87MG cells incubated for 6 h with EBSS or THC (6 µM) and subjected to subcellular fractionation in an OptiPrep® gradient. Note that MAP1LC3B-II appears in fractions of higher density in samples derived from THC-treated cells than in those derived from cells incubated with EBSS, (n = 2). (B) Analysis of the molecular species of ceramides and dihydroceramides present in the MAP1LC3B-II-enriched fraction (derived from cells treated with THC or incubated with EBSS) shown in (A). Data correspond to the ceramide:dihydroceramide ratio (upper panel) and the amount of each sphingolipid species (lower panel) in one representative experiment (n = 2). (C) Generation of ceramide rigid domains in C16 dihydroceramide-containing GUVs. Upper panel: Rigid, dihydroceramide-enriched domains (flower-like dark areas) in bilayers containing 80 mol % sn -1-palmitoyl-2-oleoyl phosphatidylcholine (POPC, a fluid phospholipid) and 20 mol % C16 dihydroceramide. Lower panel: a control experiment with a <t>C12</t> dihydroceramide that does not give rise to domains under these conditions. Bars: 10 µm. (D) Release of vesicular aqueous contents induced by ceramides. Effect of the different proportions of C16 ceramide:C16 dihydroceramide generated by the action of sphingomyelinase in LUVs composed of the following: dhSM:PC:Ch (30:67:3; red line); SM:dhSM:PC:Ch (20:10:67:3; magenta line); SM:PC:Ch (30:67:3; blue line); and SM:dhSM:PC:Ch (26:4:67:3; green line). A representative example of 3 closely similar experiments is shown. SM, sphingomyelin; dhSM, dihydrosphingomyelin; PC, phosphatidylcholine; Ch, cholesterol.
    C12, supplied by Phenomenex, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Glen Research c12
    THC, but not nutrient deprivation, decreases the <t>ceramide:dihydroceramide</t> ratio in an autophagosome-enriched fraction. (A) Characterization of the presence of the autophagy marker MAP1LC3B-II and the lysosomal marker LAMP1 in fractions obtained from U87MG cells incubated for 6 h with EBSS or THC (6 µM) and subjected to subcellular fractionation in an OptiPrep® gradient. Note that MAP1LC3B-II appears in fractions of higher density in samples derived from THC-treated cells than in those derived from cells incubated with EBSS, (n = 2). (B) Analysis of the molecular species of ceramides and dihydroceramides present in the MAP1LC3B-II-enriched fraction (derived from cells treated with THC or incubated with EBSS) shown in (A). Data correspond to the ceramide:dihydroceramide ratio (upper panel) and the amount of each sphingolipid species (lower panel) in one representative experiment (n = 2). (C) Generation of ceramide rigid domains in C16 dihydroceramide-containing GUVs. Upper panel: Rigid, dihydroceramide-enriched domains (flower-like dark areas) in bilayers containing 80 mol % sn -1-palmitoyl-2-oleoyl phosphatidylcholine (POPC, a fluid phospholipid) and 20 mol % C16 dihydroceramide. Lower panel: a control experiment with a <t>C12</t> dihydroceramide that does not give rise to domains under these conditions. Bars: 10 µm. (D) Release of vesicular aqueous contents induced by ceramides. Effect of the different proportions of C16 ceramide:C16 dihydroceramide generated by the action of sphingomyelinase in LUVs composed of the following: dhSM:PC:Ch (30:67:3; red line); SM:dhSM:PC:Ch (20:10:67:3; magenta line); SM:PC:Ch (30:67:3; blue line); and SM:dhSM:PC:Ch (26:4:67:3; green line). A representative example of 3 closely similar experiments is shown. SM, sphingomyelin; dhSM, dihydrosphingomyelin; PC, phosphatidylcholine; Ch, cholesterol.
    C12, supplied by Glen Research, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Santa Cruz Biotechnology c12
    NFAs S-alkylate Cat S specifically at Cys 25. Human recombinant Cat S WT and Cys mutants <t>C12;25;110S,</t> C12S, C25S, C110S (200 ng) were incubated for 30 min with biotin-labeled OA-NO 2 (1 μM). Following incubation, S-alkylation of Cat S by biotin-labeled OA-NO 2 was assessed by Western blotting under non-reducing conditions. Data are representative of two independent experiments.
    C12, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Avanti Polar c12 nbd ceramide
    Metabolic fate of koji glycosylceramide in the intestine. a Total lipid profile of feces from mice fed with or without koji glycosylceramide. Nor indicates the feces of non-added mice and Kgc indicates those of koji glycosylceramide-fed mice. The number indicates the replicate number of experiments. b Total lipid profile of koji glycosylceramide incubated with intestinal extracts. c <t>NBD-TLC</t> of <t>C12-ceramide</t> incubated with intestinal extracts. Intestinal extract was recovered from mice, mixed with purified koji glycosylceramide or NBD-C12-ceramide, incubated at 37 °C for 16–30 h, developed, and visualized by orcinol–H 2 SO 4 reagent or fluorescence. SG indicates Sterylglucoside and GlcCer indicates glycosylceramide, a and b indicate hydroxylated and nonhydroxylated cerebroside respectively
    C12 Nbd Ceramide, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore c12 ceramide
    Myriocin‐driven adaptive evolution alters SL composition. A. The relative content of LCBs and SLs in YPD‐grown cells (OD 600 ~ 1.0) of the LH, LH03 and LH09 strains was analysed by UPLC. Sphinganine (d17:0) and <t>C12</t> <t>Ceramide</t> (d18:1/12:0) were used as internal standards of LCBs and SLs respectively. The levels of the different lipid species in each sample were normalized to the corresponding internal standard (pmol eq) and units of processed cells (OD 600 ). Phosphorylated LCBs were below the detection limit. The quantity of SLs represents the sum of Cer, IPC and MIPC. B. The amount of Cer, IPC and MIPC sub‐classes was normalized (%) to the total content of each class. C. Hydroxylation of phytoceramide (PhC‐B) by Scs7 generates α‐OH‐phytoceramide (PhC‐C). These Cer sub‐classes are then converted to the corresponding IPC‐B and IPC‐C, and subsequently to MIPC‐B and MIPC‐C respectively. For more details, see important reviews (Huang et al. , 2014 ; Megyeri et al. , 2016 ). D. The quantities of Cer, IPC and MIPC species containing the same number of carbon atoms are summed, and these values were normalized (%) to the total amount of the corresponding SL class. In all cases, the data were calculated from at least three biological replicates (± SD). Statistically significant differences ( P
    C12 Ceramide, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Avanti Polar c12 nbd sphingomyelin
    Binding of lysenin to sphingomyelin-containing GUVs. A , shown is the observation of a GUV containing 5 mol% <t>NBD-sphingomyelin</t> ( green ) <t>(NBD-C12-SM/DOPC/SM(d18:1–16:0)/cholesterol</t> 6:33:27:33) and RFP-lysenin ( red ) ( scale bar , 10 μm). B , shown is the observation of binding of GFP-lysenin ( green ) to a GUV of DOPC/rhodamine-DOPE (molar ratio, 99:1) ( upper panel ), SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) ( middle panel ), or SM(d18:1–16:0)/DOPC/cholesterol/rhodamine-DOPE (molar ratio, 33:33:33:1) ( lower panel ). Each GUV contained 1 mol% rhodamine-DOPE ( scale bar , 10 μm). C , the fluorescence of GFP-lysenin associated with liposome ( Y circle ) and the fluorescence of GFP-lysenin outside of the liposome ( Y env ) were quantitatively measured in each GUV. The value of Y circle / Y env of the SM(d18:1- 16:0)/DOPC/cholesterol ( Chol )/rhodamine-DOPE (molar ratio, 33:33:33:1) and SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) GUVs was calculated. Data are the means ± S.D. of three independent experiments. p
    C12 Nbd Sphingomyelin, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore c12 e8
    Binding of lysenin to sphingomyelin-containing GUVs. A , shown is the observation of a GUV containing 5 mol% <t>NBD-sphingomyelin</t> ( green ) <t>(NBD-C12-SM/DOPC/SM(d18:1–16:0)/cholesterol</t> 6:33:27:33) and RFP-lysenin ( red ) ( scale bar , 10 μm). B , shown is the observation of binding of GFP-lysenin ( green ) to a GUV of DOPC/rhodamine-DOPE (molar ratio, 99:1) ( upper panel ), SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) ( middle panel ), or SM(d18:1–16:0)/DOPC/cholesterol/rhodamine-DOPE (molar ratio, 33:33:33:1) ( lower panel ). Each GUV contained 1 mol% rhodamine-DOPE ( scale bar , 10 μm). C , the fluorescence of GFP-lysenin associated with liposome ( Y circle ) and the fluorescence of GFP-lysenin outside of the liposome ( Y env ) were quantitatively measured in each GUV. The value of Y circle / Y env of the SM(d18:1- 16:0)/DOPC/cholesterol ( Chol )/rhodamine-DOPE (molar ratio, 33:33:33:1) and SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) GUVs was calculated. Data are the means ± S.D. of three independent experiments. p
    C12 E8, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher c12 e8
    Binding of lysenin to sphingomyelin-containing GUVs. A , shown is the observation of a GUV containing 5 mol% <t>NBD-sphingomyelin</t> ( green ) <t>(NBD-C12-SM/DOPC/SM(d18:1–16:0)/cholesterol</t> 6:33:27:33) and RFP-lysenin ( red ) ( scale bar , 10 μm). B , shown is the observation of binding of GFP-lysenin ( green ) to a GUV of DOPC/rhodamine-DOPE (molar ratio, 99:1) ( upper panel ), SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) ( middle panel ), or SM(d18:1–16:0)/DOPC/cholesterol/rhodamine-DOPE (molar ratio, 33:33:33:1) ( lower panel ). Each GUV contained 1 mol% rhodamine-DOPE ( scale bar , 10 μm). C , the fluorescence of GFP-lysenin associated with liposome ( Y circle ) and the fluorescence of GFP-lysenin outside of the liposome ( Y env ) were quantitatively measured in each GUV. The value of Y circle / Y env of the SM(d18:1- 16:0)/DOPC/cholesterol ( Chol )/rhodamine-DOPE (molar ratio, 33:33:33:1) and SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) GUVs was calculated. Data are the means ± S.D. of three independent experiments. p
    C12 E8, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore c12 fdg
    Binding of lysenin to sphingomyelin-containing GUVs. A , shown is the observation of a GUV containing 5 mol% <t>NBD-sphingomyelin</t> ( green ) <t>(NBD-C12-SM/DOPC/SM(d18:1–16:0)/cholesterol</t> 6:33:27:33) and RFP-lysenin ( red ) ( scale bar , 10 μm). B , shown is the observation of binding of GFP-lysenin ( green ) to a GUV of DOPC/rhodamine-DOPE (molar ratio, 99:1) ( upper panel ), SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) ( middle panel ), or SM(d18:1–16:0)/DOPC/cholesterol/rhodamine-DOPE (molar ratio, 33:33:33:1) ( lower panel ). Each GUV contained 1 mol% rhodamine-DOPE ( scale bar , 10 μm). C , the fluorescence of GFP-lysenin associated with liposome ( Y circle ) and the fluorescence of GFP-lysenin outside of the liposome ( Y env ) were quantitatively measured in each GUV. The value of Y circle / Y env of the SM(d18:1- 16:0)/DOPC/cholesterol ( Chol )/rhodamine-DOPE (molar ratio, 33:33:33:1) and SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) GUVs was calculated. Data are the means ± S.D. of three independent experiments. p
    C12 Fdg, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore c12 hsl
    Dose response of 3-Oxo-N and competition between 3-Oxo-N and <t>C12-HSL.</t> a) Total biofilm formation and total lactic acid accumulation for biofilms grown in the presence of different concentrations of 3-oxo- N -(2-oxocyclohexyl)dodecanamide. White bars: biofilm formation expressed as CFU/biofilm; black bars: lactic acid accumulation expressed in mM per biofilm after 3-h incubation in BPW containing 0.2% sucrose. Statistical significance compared to the control is shown (* P
    C12 Hsl, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Phenomenex c12 material
    Dose response of 3-Oxo-N and competition between 3-Oxo-N and <t>C12-HSL.</t> a) Total biofilm formation and total lactic acid accumulation for biofilms grown in the presence of different concentrations of 3-oxo- N -(2-oxocyclohexyl)dodecanamide. White bars: biofilm formation expressed as CFU/biofilm; black bars: lactic acid accumulation expressed in mM per biofilm after 3-h incubation in BPW containing 0.2% sucrose. Statistical significance compared to the control is shown (* P
    C12 Material, supplied by Phenomenex, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore c12 0
    Dose response of 3-Oxo-N and competition between 3-Oxo-N and <t>C12-HSL.</t> a) Total biofilm formation and total lactic acid accumulation for biofilms grown in the presence of different concentrations of 3-oxo- N -(2-oxocyclohexyl)dodecanamide. White bars: biofilm formation expressed as CFU/biofilm; black bars: lactic acid accumulation expressed in mM per biofilm after 3-h incubation in BPW containing 0.2% sucrose. Statistical significance compared to the control is shown (* P
    C12 0, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher c12 fdg
    Dose response of 3-Oxo-N and competition between 3-Oxo-N and <t>C12-HSL.</t> a) Total biofilm formation and total lactic acid accumulation for biofilms grown in the presence of different concentrations of 3-oxo- N -(2-oxocyclohexyl)dodecanamide. White bars: biofilm formation expressed as CFU/biofilm; black bars: lactic acid accumulation expressed in mM per biofilm after 3-h incubation in BPW containing 0.2% sucrose. Statistical significance compared to the control is shown (* P
    C12 Fdg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Wellington Laboratories c12 tcs
    Dose response of 3-Oxo-N and competition between 3-Oxo-N and <t>C12-HSL.</t> a) Total biofilm formation and total lactic acid accumulation for biofilms grown in the presence of different concentrations of 3-oxo- N -(2-oxocyclohexyl)dodecanamide. White bars: biofilm formation expressed as CFU/biofilm; black bars: lactic acid accumulation expressed in mM per biofilm after 3-h incubation in BPW containing 0.2% sucrose. Statistical significance compared to the control is shown (* P
    C12 Tcs, supplied by Wellington Laboratories, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology lgr4 c 12
    Dose response of 3-Oxo-N and competition between 3-Oxo-N and <t>C12-HSL.</t> a) Total biofilm formation and total lactic acid accumulation for biofilms grown in the presence of different concentrations of 3-oxo- N -(2-oxocyclohexyl)dodecanamide. White bars: biofilm formation expressed as CFU/biofilm; black bars: lactic acid accumulation expressed in mM per biofilm after 3-h incubation in BPW containing 0.2% sucrose. Statistical significance compared to the control is shown (* P
    Lgr4 C 12, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Nutrient transport and brush border membrane expression. 58 Fe transport (A and B) or uptake (E, F), 67 Zn transport (C and D) or uptake (G, H), fluorescent BODIPY® 500/510 C1, C12 lipid uptake (I and J), and alkaline phosphatase activity (K and L) in response to acute (A, C, E, G, I, K) and chronic (B, D, F, H, J, L) doses of TiO 2 . Data is mean±SEM. * denotes significance according to an unpaired Student’s t-test, p

    Journal: NanoImpact

    Article Title: Titanium Dioxide Nanoparticle Ingestion Alters Nutrient Absorption in an In Vitro Model of the Small Intestine

    doi: 10.1016/j.impact.2017.01.002

    Figure Lengend Snippet: Nutrient transport and brush border membrane expression. 58 Fe transport (A and B) or uptake (E, F), 67 Zn transport (C and D) or uptake (G, H), fluorescent BODIPY® 500/510 C1, C12 lipid uptake (I and J), and alkaline phosphatase activity (K and L) in response to acute (A, C, E, G, I, K) and chronic (B, D, F, H, J, L) doses of TiO 2 . Data is mean±SEM. * denotes significance according to an unpaired Student’s t-test, p

    Article Snippet: Cellular uptake studies of free fatty acids were performed using fluorescent BODIPY® 500/510 C1, C12 (4, 4-Difluoro-5-Methyl-4-Bora-3a, 4a-Diaza-s-Indacene-3-Dodecanoic Acid, ThermoFisher) – .

    Techniques: Expressing, Activity Assay

    Transduction efficiency. Flow cytometry analysis of C12 cells infected with equal vector genome amounts of transfection or infection made recombinant adeno-associated virus (rAAV)9-GFP. Purified vectors preparations obtained by transfection ( n = 4) or infection ( n = 5) were pooled and used to infected cells at various multiplicity of infections and GFP expression detected by FACS10. Average from duplicates with standard deviation are depicted.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: A scalable method for the production of high-titer and high-quality adeno-associated type 9 vectors using the HSV platform

    doi: 10.1038/mtm.2016.31

    Figure Lengend Snippet: Transduction efficiency. Flow cytometry analysis of C12 cells infected with equal vector genome amounts of transfection or infection made recombinant adeno-associated virus (rAAV)9-GFP. Purified vectors preparations obtained by transfection ( n = 4) or infection ( n = 5) were pooled and used to infected cells at various multiplicity of infections and GFP expression detected by FACS10. Average from duplicates with standard deviation are depicted.

    Article Snippet: V27 (a gift from Dr. Knipe, ) and C12 (a gift from Dr. P. Johnson, cells were maintained in DMEM supplemented with 5% FBS, and 50 μg/ml Geneticin (Sigma, St. Louis, MO).

    Techniques: Transduction, Flow Cytometry, Cytometry, Infection, Plasmid Preparation, Transfection, Recombinant, Purification, Expressing, Standard Deviation

    THC, but not nutrient deprivation, decreases the ceramide:dihydroceramide ratio in an autophagosome-enriched fraction. (A) Characterization of the presence of the autophagy marker MAP1LC3B-II and the lysosomal marker LAMP1 in fractions obtained from U87MG cells incubated for 6 h with EBSS or THC (6 µM) and subjected to subcellular fractionation in an OptiPrep® gradient. Note that MAP1LC3B-II appears in fractions of higher density in samples derived from THC-treated cells than in those derived from cells incubated with EBSS, (n = 2). (B) Analysis of the molecular species of ceramides and dihydroceramides present in the MAP1LC3B-II-enriched fraction (derived from cells treated with THC or incubated with EBSS) shown in (A). Data correspond to the ceramide:dihydroceramide ratio (upper panel) and the amount of each sphingolipid species (lower panel) in one representative experiment (n = 2). (C) Generation of ceramide rigid domains in C16 dihydroceramide-containing GUVs. Upper panel: Rigid, dihydroceramide-enriched domains (flower-like dark areas) in bilayers containing 80 mol % sn -1-palmitoyl-2-oleoyl phosphatidylcholine (POPC, a fluid phospholipid) and 20 mol % C16 dihydroceramide. Lower panel: a control experiment with a C12 dihydroceramide that does not give rise to domains under these conditions. Bars: 10 µm. (D) Release of vesicular aqueous contents induced by ceramides. Effect of the different proportions of C16 ceramide:C16 dihydroceramide generated by the action of sphingomyelinase in LUVs composed of the following: dhSM:PC:Ch (30:67:3; red line); SM:dhSM:PC:Ch (20:10:67:3; magenta line); SM:PC:Ch (30:67:3; blue line); and SM:dhSM:PC:Ch (26:4:67:3; green line). A representative example of 3 closely similar experiments is shown. SM, sphingomyelin; dhSM, dihydrosphingomyelin; PC, phosphatidylcholine; Ch, cholesterol.

    Journal: Autophagy

    Article Title: Dihydroceramide accumulation mediates cytotoxic autophagy of cancer cells via autolysosome destabilization

    doi: 10.1080/15548627.2016.1213927

    Figure Lengend Snippet: THC, but not nutrient deprivation, decreases the ceramide:dihydroceramide ratio in an autophagosome-enriched fraction. (A) Characterization of the presence of the autophagy marker MAP1LC3B-II and the lysosomal marker LAMP1 in fractions obtained from U87MG cells incubated for 6 h with EBSS or THC (6 µM) and subjected to subcellular fractionation in an OptiPrep® gradient. Note that MAP1LC3B-II appears in fractions of higher density in samples derived from THC-treated cells than in those derived from cells incubated with EBSS, (n = 2). (B) Analysis of the molecular species of ceramides and dihydroceramides present in the MAP1LC3B-II-enriched fraction (derived from cells treated with THC or incubated with EBSS) shown in (A). Data correspond to the ceramide:dihydroceramide ratio (upper panel) and the amount of each sphingolipid species (lower panel) in one representative experiment (n = 2). (C) Generation of ceramide rigid domains in C16 dihydroceramide-containing GUVs. Upper panel: Rigid, dihydroceramide-enriched domains (flower-like dark areas) in bilayers containing 80 mol % sn -1-palmitoyl-2-oleoyl phosphatidylcholine (POPC, a fluid phospholipid) and 20 mol % C16 dihydroceramide. Lower panel: a control experiment with a C12 dihydroceramide that does not give rise to domains under these conditions. Bars: 10 µm. (D) Release of vesicular aqueous contents induced by ceramides. Effect of the different proportions of C16 ceramide:C16 dihydroceramide generated by the action of sphingomyelinase in LUVs composed of the following: dhSM:PC:Ch (30:67:3; red line); SM:dhSM:PC:Ch (20:10:67:3; magenta line); SM:PC:Ch (30:67:3; blue line); and SM:dhSM:PC:Ch (26:4:67:3; green line). A representative example of 3 closely similar experiments is shown. SM, sphingomyelin; dhSM, dihydrosphingomyelin; PC, phosphatidylcholine; Ch, cholesterol.

    Article Snippet: Phosphatidylcholine from egg yolk (PC; Lipid Products, grade 1, 840051P), sphingomyelin, (SM; Avanti Polar Lipids, 860061), C12 ceramide (C12-Cer; Avanti Polar Lipids, 860512), C16 dihydroceramide (C16-dhCer; Avanti Polar Lipids, 860634), palmitoyl-oleoylphosphatidylcholine (POPC; Avanti Polar Lipids, 850457) and cholesterol (Ch; Avanti Polar Lipids, 700000).

    Techniques: Marker, Incubation, Fractionation, Derivative Assay, Generated

    NFAs S-alkylate Cat S specifically at Cys 25. Human recombinant Cat S WT and Cys mutants C12;25;110S, C12S, C25S, C110S (200 ng) were incubated for 30 min with biotin-labeled OA-NO 2 (1 μM). Following incubation, S-alkylation of Cat S by biotin-labeled OA-NO 2 was assessed by Western blotting under non-reducing conditions. Data are representative of two independent experiments.

    Journal: PLoS ONE

    Article Title: Nitrated Fatty Acids Reverse Cigarette Smoke-Induced Alveolar Macrophage Activation and Inhibit Protease Activity via Electrophilic S-Alkylation

    doi: 10.1371/journal.pone.0153336

    Figure Lengend Snippet: NFAs S-alkylate Cat S specifically at Cys 25. Human recombinant Cat S WT and Cys mutants C12;25;110S, C12S, C25S, C110S (200 ng) were incubated for 30 min with biotin-labeled OA-NO 2 (1 μM). Following incubation, S-alkylation of Cat S by biotin-labeled OA-NO 2 was assessed by Western blotting under non-reducing conditions. Data are representative of two independent experiments.

    Article Snippet: Cat S S-alkylation To demonstrate S-alkylation of Cat S, human recombinant Cat S (EMD Millipore Billerica, MA) and variants−Cat S WT, C12;25;110S mutant, C12S mutant, C25S mutant, and C110S mutant−were incubated with test compounds for the indicated time periods and S-alkylation was analyzed by running these samples in a SDS-PAGE gel under non-reducing conditions and performing Western blots using antibody to biotin (Santa Cruz Biotechnology).

    Techniques: Recombinant, Incubation, Labeling, Western Blot

    Metabolic fate of koji glycosylceramide in the intestine. a Total lipid profile of feces from mice fed with or without koji glycosylceramide. Nor indicates the feces of non-added mice and Kgc indicates those of koji glycosylceramide-fed mice. The number indicates the replicate number of experiments. b Total lipid profile of koji glycosylceramide incubated with intestinal extracts. c NBD-TLC of C12-ceramide incubated with intestinal extracts. Intestinal extract was recovered from mice, mixed with purified koji glycosylceramide or NBD-C12-ceramide, incubated at 37 °C for 16–30 h, developed, and visualized by orcinol–H 2 SO 4 reagent or fluorescence. SG indicates Sterylglucoside and GlcCer indicates glycosylceramide, a and b indicate hydroxylated and nonhydroxylated cerebroside respectively

    Journal: SpringerPlus

    Article Title: Japanese traditional dietary fungus koji Aspergillus oryzae functions as a prebiotic for Blautia coccoides through glycosylceramide: Japanese dietary fungus koji is a new prebiotic

    doi: 10.1186/s40064-016-2950-6

    Figure Lengend Snippet: Metabolic fate of koji glycosylceramide in the intestine. a Total lipid profile of feces from mice fed with or without koji glycosylceramide. Nor indicates the feces of non-added mice and Kgc indicates those of koji glycosylceramide-fed mice. The number indicates the replicate number of experiments. b Total lipid profile of koji glycosylceramide incubated with intestinal extracts. c NBD-TLC of C12-ceramide incubated with intestinal extracts. Intestinal extract was recovered from mice, mixed with purified koji glycosylceramide or NBD-C12-ceramide, incubated at 37 °C for 16–30 h, developed, and visualized by orcinol–H 2 SO 4 reagent or fluorescence. SG indicates Sterylglucoside and GlcCer indicates glycosylceramide, a and b indicate hydroxylated and nonhydroxylated cerebroside respectively

    Article Snippet: Ceramidase activity was measured using C12-NBD-ceramide (Avanti Polar Lipids, Inc., AL, USA) as the substrate (Mitsutake et al. ).

    Techniques: Mouse Assay, Incubation, Thin Layer Chromatography, Purification, Fluorescence

    Myriocin‐driven adaptive evolution alters SL composition. A. The relative content of LCBs and SLs in YPD‐grown cells (OD 600 ~ 1.0) of the LH, LH03 and LH09 strains was analysed by UPLC. Sphinganine (d17:0) and C12 Ceramide (d18:1/12:0) were used as internal standards of LCBs and SLs respectively. The levels of the different lipid species in each sample were normalized to the corresponding internal standard (pmol eq) and units of processed cells (OD 600 ). Phosphorylated LCBs were below the detection limit. The quantity of SLs represents the sum of Cer, IPC and MIPC. B. The amount of Cer, IPC and MIPC sub‐classes was normalized (%) to the total content of each class. C. Hydroxylation of phytoceramide (PhC‐B) by Scs7 generates α‐OH‐phytoceramide (PhC‐C). These Cer sub‐classes are then converted to the corresponding IPC‐B and IPC‐C, and subsequently to MIPC‐B and MIPC‐C respectively. For more details, see important reviews (Huang et al. , 2014 ; Megyeri et al. , 2016 ). D. The quantities of Cer, IPC and MIPC species containing the same number of carbon atoms are summed, and these values were normalized (%) to the total amount of the corresponding SL class. In all cases, the data were calculated from at least three biological replicates (± SD). Statistically significant differences ( P

    Journal: Microbial Biotechnology

    Article Title: Myriocin‐induced adaptive laboratory evolution of an industrial strain of Saccharomyces cerevisiae reveals its potential to remodel lipid composition and heat tolerance

    doi: 10.1111/1751-7915.13555

    Figure Lengend Snippet: Myriocin‐driven adaptive evolution alters SL composition. A. The relative content of LCBs and SLs in YPD‐grown cells (OD 600 ~ 1.0) of the LH, LH03 and LH09 strains was analysed by UPLC. Sphinganine (d17:0) and C12 Ceramide (d18:1/12:0) were used as internal standards of LCBs and SLs respectively. The levels of the different lipid species in each sample were normalized to the corresponding internal standard (pmol eq) and units of processed cells (OD 600 ). Phosphorylated LCBs were below the detection limit. The quantity of SLs represents the sum of Cer, IPC and MIPC. B. The amount of Cer, IPC and MIPC sub‐classes was normalized (%) to the total content of each class. C. Hydroxylation of phytoceramide (PhC‐B) by Scs7 generates α‐OH‐phytoceramide (PhC‐C). These Cer sub‐classes are then converted to the corresponding IPC‐B and IPC‐C, and subsequently to MIPC‐B and MIPC‐C respectively. For more details, see important reviews (Huang et al. , 2014 ; Megyeri et al. , 2016 ). D. The quantities of Cer, IPC and MIPC species containing the same number of carbon atoms are summed, and these values were normalized (%) to the total amount of the corresponding SL class. In all cases, the data were calculated from at least three biological replicates (± SD). Statistically significant differences ( P

    Article Snippet: Methanol (1 ml) and chloroform (0.5 ml) were added to 0.5 ml of the cell suspension, fortified with internal standards [200 pmol: sphinganine (d17:0), sphinganine‐1‐phosphate (d17:0), and C12 Ceramide (d18:1/12:0)], vortexed and incubated at 48°C overnight.

    Techniques:

    Binding of lysenin to sphingomyelin-containing GUVs. A , shown is the observation of a GUV containing 5 mol% NBD-sphingomyelin ( green ) (NBD-C12-SM/DOPC/SM(d18:1–16:0)/cholesterol 6:33:27:33) and RFP-lysenin ( red ) ( scale bar , 10 μm). B , shown is the observation of binding of GFP-lysenin ( green ) to a GUV of DOPC/rhodamine-DOPE (molar ratio, 99:1) ( upper panel ), SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) ( middle panel ), or SM(d18:1–16:0)/DOPC/cholesterol/rhodamine-DOPE (molar ratio, 33:33:33:1) ( lower panel ). Each GUV contained 1 mol% rhodamine-DOPE ( scale bar , 10 μm). C , the fluorescence of GFP-lysenin associated with liposome ( Y circle ) and the fluorescence of GFP-lysenin outside of the liposome ( Y env ) were quantitatively measured in each GUV. The value of Y circle / Y env of the SM(d18:1- 16:0)/DOPC/cholesterol ( Chol )/rhodamine-DOPE (molar ratio, 33:33:33:1) and SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) GUVs was calculated. Data are the means ± S.D. of three independent experiments. p

    Journal: The Journal of Biological Chemistry

    Article Title: Lipid Polarity Is Maintained in Absence of Tight Junctions

    doi: 10.1074/jbc.M111.327064

    Figure Lengend Snippet: Binding of lysenin to sphingomyelin-containing GUVs. A , shown is the observation of a GUV containing 5 mol% NBD-sphingomyelin ( green ) (NBD-C12-SM/DOPC/SM(d18:1–16:0)/cholesterol 6:33:27:33) and RFP-lysenin ( red ) ( scale bar , 10 μm). B , shown is the observation of binding of GFP-lysenin ( green ) to a GUV of DOPC/rhodamine-DOPE (molar ratio, 99:1) ( upper panel ), SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) ( middle panel ), or SM(d18:1–16:0)/DOPC/cholesterol/rhodamine-DOPE (molar ratio, 33:33:33:1) ( lower panel ). Each GUV contained 1 mol% rhodamine-DOPE ( scale bar , 10 μm). C , the fluorescence of GFP-lysenin associated with liposome ( Y circle ) and the fluorescence of GFP-lysenin outside of the liposome ( Y env ) were quantitatively measured in each GUV. The value of Y circle / Y env of the SM(d18:1- 16:0)/DOPC/cholesterol ( Chol )/rhodamine-DOPE (molar ratio, 33:33:33:1) and SM (d18:1–16:0)/DOPC/rhodamine-DOPE (molar ratio, 49:50:1) GUVs was calculated. Data are the means ± S.D. of three independent experiments. p

    Article Snippet: The DOPC (1,2-di-oleoyl-sn-glycero-3-phosphocholine), sphingomyelin (d18:1–16:0) N -palmitoyl- d - erythro -sphingosylphosphorylcholine), cholesterol, rhodamine-DOPE (1,2-dioleoyl- sn -glycero-3-phosphoethanolamine- n -(lissamine rhodamine B sulfonyl)), C12-NBD-sphingomyelin ( N -[12-[(7-nitro-2–1,3-benzoxadiazol-4-yl)amino]lauroyl]-sphingosine-1-phosphocholine), and 18:1–06:0 NBD PS 1-oleoyl-2-{6-[(7-nitro-2–1,3-benzoxadiazol-4-yl)amino]hexanoyl}- sn -glycero-3-phosphoserine were obtained from Avanti Polar Lipids (Alabaster, AL).

    Techniques: Binding Assay, Fluorescence

    Dose response of 3-Oxo-N and competition between 3-Oxo-N and C12-HSL. a) Total biofilm formation and total lactic acid accumulation for biofilms grown in the presence of different concentrations of 3-oxo- N -(2-oxocyclohexyl)dodecanamide. White bars: biofilm formation expressed as CFU/biofilm; black bars: lactic acid accumulation expressed in mM per biofilm after 3-h incubation in BPW containing 0.2% sucrose. Statistical significance compared to the control is shown (* P

    Journal: Journal of Oral Microbiology

    Article Title: A novel compound to maintain a healthy oral plaque ecology in vitro

    doi: 10.3402/jom.v8.32513

    Figure Lengend Snippet: Dose response of 3-Oxo-N and competition between 3-Oxo-N and C12-HSL. a) Total biofilm formation and total lactic acid accumulation for biofilms grown in the presence of different concentrations of 3-oxo- N -(2-oxocyclohexyl)dodecanamide. White bars: biofilm formation expressed as CFU/biofilm; black bars: lactic acid accumulation expressed in mM per biofilm after 3-h incubation in BPW containing 0.2% sucrose. Statistical significance compared to the control is shown (* P

    Article Snippet: All compounds , except DPD and C12-HSL, were obtained from Sigma Aldrich (St. Louis, MO).

    Techniques: Incubation