Journal: The Journal of Biological Chemistry

Article Title: A myelin basic protein fragment induces sexually dimorphic transcriptome signatures of neuropathic pain in mice

doi: 10.1074/jbc.RA120.013696

Figure Lengend Snippet: IS MBP(84-104) causes striking sexual dimorphism in spinal nociceptive signaling. a, neuropathic pain network of PLC/IP3R/VGCC axis and ESR1/p300/CBP axis after IS MBP(84-104) in females (left panel) and males (right panel). Pathway components (molecules and complexes) are displayed as circles, arrows indicate molecular interactions according to Ingenuity IPA (Qiagen). Downstream biological processes are highlighted in blue. Red and green colors correspond to up- and down-regulated DE genes according to the color scale (log2FC). Gradient colors indicate regulation of individual components of protein complexes. Threshold FC ≥ 2, p ≤ 0.05. IP3R is a site of xestospongin C action in females (orange line). b, von Frey testing in female mice ipsilateral (left panel) and contralateral (right panel) to IS MBP(84-104) (30 μg in 3 μl). At day 10 after IS MBP(84-104), IT administered IP3R inhibitor, xestospongin C (X2628, 1 nmol/5 µl, n = 5), but not vehicle 10% DMSO (5 µl, n = 4), attenuated the established allodynia. Control i.p. gabapentin (100 mg/kg, n = 3) also attenuated IS MBP(84-104) allodynia. The mean withdrawal tactile thresholds are in gram force (g) ± S.E.; two-way ANOVA (b, left: treatment × time f = 3.220, p = 0.0162, time f = 9.681, p = 0.0002, treatment f = 15.94, p = 0.0011, subject f = 1.125, p = 0.3797; b, right: treatment × time f = 1.407, p = 0.2482, time f = 1.346, p = 0.2803, treatment f = 0.2114, p = 0.8134, subject f = 3.109, p = 0.0107) with Dunnett's post hoc test: *, p ≤ 0.05; **, p ≤ 0.01; ****, p ≤ 0.0001 relative to the DMSO control. Not all groups were tested at the 15- and 45-min time points; these time points are shown in the graph, but were not included in the data analysis. Red arrows indicate drug administration.

Article Snippet: Gabapentin was from Toronto Research Chemicals (Ontario, Canada), and xestospongin C (X2628) was from Sigma-Aldrich.

Techniques: Control

Journal: Molecular Pharmaceutics

Article Title: Role of the Phospholipase A 2 Receptor in Liposome Drug Delivery in Prostate Cancer Cells

doi: 10.1021/mp500174p

Figure Lengend Snippet: Effect of PLA2R1 knockdown on chemotherapeutic-induced toxicity in PC-3 cells. Cells were treated with docetaxel (A), cisplatin (B), or Dox (C) for 72 h. MTT assays were used to determine the effect of knocking down PLA2R1 on chemotherapeutic-induced cytotoxicity. The effect of PLA2R1 on doxorubicin-induced toxicity was further assessed at 72 h using phase contrast microscopy at 10× magnification (D). Data in panels A–C are represented as the mean ± SD of at least three separate experiments ( n = 3/study). Data in panel D are indicative of at least three separate experiments.

Article Snippet: Doxorubicin (Dox) was purchased from Toronto Research Chemicals (North York, ON, Canada).

Techniques: Knockdown, Microscopy

Journal: Molecular Pharmaceutics

Article Title: Role of the Phospholipase A 2 Receptor in Liposome Drug Delivery in Prostate Cancer Cells

doi: 10.1021/mp500174p

Figure Lengend Snippet: Effect of PLA2R1 knockdown on the toxicity of doxorubicin encapsulated in SSL and SPRL in PC-3 cells. PLA2R1 knockdown cells and those expressing scrambled shRNA were treated with 2.5 μM concentrations of Dox encapsulated in SSL or SPRL. MTT assays (A) and phase contrast microscopy at 40× magnification (B) were used to determine the effect of PLA2R1 knockdown on cytotoxicity. Data in panel A are represented as the mean ± SEM of at least three separate experiments ( n = 3/study). *Indicates a significant difference ( p < 0.05) as compared to cells transfected with scrambled shRNA. Data in panel E are indicative of at least three separate experiments.

Article Snippet: Doxorubicin (Dox) was purchased from Toronto Research Chemicals (North York, ON, Canada).

Techniques: Knockdown, Expressing, shRNA, Microscopy, Transfection

Journal: Molecular Pharmaceutics

Article Title: Role of the Phospholipase A 2 Receptor in Liposome Drug Delivery in Prostate Cancer Cells

doi: 10.1021/mp500174p

Figure Lengend Snippet: Effect of PLA2R1 knockdown on DiO and doxorubicin uptake from SSL and SPRL in PC-3 cells. PC-3 cells were treated with liposomes containing Dox and DiO for 24 to 72 h. The efficiency of DiO and drug uptake via SSL (A, B) and SPRL (C, D) was quantified using flow cytometry. Data are represented as the mean ± SEM of at least three separate experiments ( n = 3/study). *Indicates a significant difference ( p < 0.05) as compared to cells expressing scrambled shRNA.

Article Snippet: Doxorubicin (Dox) was purchased from Toronto Research Chemicals (North York, ON, Canada).

Techniques: Knockdown, Liposomes, Flow Cytometry, Expressing, shRNA