built-in function anovan Search Results


prisms software  (GraphPad Software Inc)
90
GraphPad Software Inc prisms software
Representative antibody pair screening experiment. Recombinant SARS-CoV-2 NP was serially diluted and probed on pads. ( a ) A representative protein microarray image showing all 16 pads which are consisted of identical arrays of proteins. The brightness of the image was adjusted from 0 to 15,000 using ImageJ. ( b ) A representative image of a single pad is shown with labels. Each label indicates the following reagents: label 1—fiducials, label 2, 3, 5, and 6—anti-SARS-CoV-2 NP capture antibodies (reagent number 8, 14, 11, and 12 in , respectively), and label 4—recombinant SARS-CoV-2 NP (reagent number 1 in ). The anti-SARS-CoV-2 NP capture antibodies were printed at different concentrations in duplicates. Fluorescence signals captured from the highest printing concentrations (red box) were used from each pad to calculate K D and Bmax. ( c ) A representative graph after curve-fitting using the antibody pair 1 in is shown. Recombinant SARS-CoV-2 NP was serially diluted in a five-fold and probed on protein microarray. A total of ten concentration points were tested. After probing, fluorescence signals from each concentration point were measured and recorded. Using a built-in function in the <t>PRISM</t> <t>software</t> (nonlinear regression, one site—specific binding), K D and Bmax were calculated, which were used for the main parameters to determine the best antibody pair to detect SARS-CoV-2 NP on the protein microarray. Some error bars were not present on the graph because they were too small to be drawn. A single experiment with two technical replicates was performed for each antibody pair.
Prisms Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prisms software/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
prisms software - by Bioz Stars, 2026-05
90/100 stars
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one-way anova analysis  (GraphPad Software Inc)
90
GraphPad Software Inc one-way anova analysis
Representative antibody pair screening experiment. Recombinant SARS-CoV-2 NP was serially diluted and probed on pads. ( a ) A representative protein microarray image showing all 16 pads which are consisted of identical arrays of proteins. The brightness of the image was adjusted from 0 to 15,000 using ImageJ. ( b ) A representative image of a single pad is shown with labels. Each label indicates the following reagents: label 1—fiducials, label 2, 3, 5, and 6—anti-SARS-CoV-2 NP capture antibodies (reagent number 8, 14, 11, and 12 in , respectively), and label 4—recombinant SARS-CoV-2 NP (reagent number 1 in ). The anti-SARS-CoV-2 NP capture antibodies were printed at different concentrations in duplicates. Fluorescence signals captured from the highest printing concentrations (red box) were used from each pad to calculate K D and Bmax. ( c ) A representative graph after curve-fitting using the antibody pair 1 in is shown. Recombinant SARS-CoV-2 NP was serially diluted in a five-fold and probed on protein microarray. A total of ten concentration points were tested. After probing, fluorescence signals from each concentration point were measured and recorded. Using a built-in function in the <t>PRISM</t> <t>software</t> (nonlinear regression, one site—specific binding), K D and Bmax were calculated, which were used for the main parameters to determine the best antibody pair to detect SARS-CoV-2 NP on the protein microarray. Some error bars were not present on the graph because they were too small to be drawn. A single experiment with two technical replicates was performed for each antibody pair.
One Way Anova Analysis, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/one-way anova analysis/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
one-way anova analysis - by Bioz Stars, 2026-05
90/100 stars
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prism7  (GraphPad Software Inc)
90
GraphPad Software Inc prism7
Representative antibody pair screening experiment. Recombinant SARS-CoV-2 NP was serially diluted and probed on pads. ( a ) A representative protein microarray image showing all 16 pads which are consisted of identical arrays of proteins. The brightness of the image was adjusted from 0 to 15,000 using ImageJ. ( b ) A representative image of a single pad is shown with labels. Each label indicates the following reagents: label 1—fiducials, label 2, 3, 5, and 6—anti-SARS-CoV-2 NP capture antibodies (reagent number 8, 14, 11, and 12 in , respectively), and label 4—recombinant SARS-CoV-2 NP (reagent number 1 in ). The anti-SARS-CoV-2 NP capture antibodies were printed at different concentrations in duplicates. Fluorescence signals captured from the highest printing concentrations (red box) were used from each pad to calculate K D and Bmax. ( c ) A representative graph after curve-fitting using the antibody pair 1 in is shown. Recombinant SARS-CoV-2 NP was serially diluted in a five-fold and probed on protein microarray. A total of ten concentration points were tested. After probing, fluorescence signals from each concentration point were measured and recorded. Using a built-in function in the <t>PRISM</t> <t>software</t> (nonlinear regression, one site—specific binding), K D and Bmax were calculated, which were used for the main parameters to determine the best antibody pair to detect SARS-CoV-2 NP on the protein microarray. Some error bars were not present on the graph because they were too small to be drawn. A single experiment with two technical replicates was performed for each antibody pair.
Prism7, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prism7/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
prism7 - by Bioz Stars, 2026-05
90/100 stars
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built-in function in origin pro 8.1  (OriginLab corp)
90
OriginLab corp built-in function in origin pro 8.1
Representative antibody pair screening experiment. Recombinant SARS-CoV-2 NP was serially diluted and probed on pads. ( a ) A representative protein microarray image showing all 16 pads which are consisted of identical arrays of proteins. The brightness of the image was adjusted from 0 to 15,000 using ImageJ. ( b ) A representative image of a single pad is shown with labels. Each label indicates the following reagents: label 1—fiducials, label 2, 3, 5, and 6—anti-SARS-CoV-2 NP capture antibodies (reagent number 8, 14, 11, and 12 in , respectively), and label 4—recombinant SARS-CoV-2 NP (reagent number 1 in ). The anti-SARS-CoV-2 NP capture antibodies were printed at different concentrations in duplicates. Fluorescence signals captured from the highest printing concentrations (red box) were used from each pad to calculate K D and Bmax. ( c ) A representative graph after curve-fitting using the antibody pair 1 in is shown. Recombinant SARS-CoV-2 NP was serially diluted in a five-fold and probed on protein microarray. A total of ten concentration points were tested. After probing, fluorescence signals from each concentration point were measured and recorded. Using a built-in function in the <t>PRISM</t> <t>software</t> (nonlinear regression, one site—specific binding), K D and Bmax were calculated, which were used for the main parameters to determine the best antibody pair to detect SARS-CoV-2 NP on the protein microarray. Some error bars were not present on the graph because they were too small to be drawn. A single experiment with two technical replicates was performed for each antibody pair.
Built In Function In Origin Pro 8.1, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/built-in function in origin pro 8.1/product/OriginLab corp
Average 90 stars, based on 1 article reviews
built-in function in origin pro 8.1 - by Bioz Stars, 2026-05
90/100 stars
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graphpad prism 10  (GraphPad Software Inc)
90
GraphPad Software Inc graphpad prism 10
Representative antibody pair screening experiment. Recombinant SARS-CoV-2 NP was serially diluted and probed on pads. ( a ) A representative protein microarray image showing all 16 pads which are consisted of identical arrays of proteins. The brightness of the image was adjusted from 0 to 15,000 using ImageJ. ( b ) A representative image of a single pad is shown with labels. Each label indicates the following reagents: label 1—fiducials, label 2, 3, 5, and 6—anti-SARS-CoV-2 NP capture antibodies (reagent number 8, 14, 11, and 12 in , respectively), and label 4—recombinant SARS-CoV-2 NP (reagent number 1 in ). The anti-SARS-CoV-2 NP capture antibodies were printed at different concentrations in duplicates. Fluorescence signals captured from the highest printing concentrations (red box) were used from each pad to calculate K D and Bmax. ( c ) A representative graph after curve-fitting using the antibody pair 1 in is shown. Recombinant SARS-CoV-2 NP was serially diluted in a five-fold and probed on protein microarray. A total of ten concentration points were tested. After probing, fluorescence signals from each concentration point were measured and recorded. Using a built-in function in the <t>PRISM</t> <t>software</t> (nonlinear regression, one site—specific binding), K D and Bmax were calculated, which were used for the main parameters to determine the best antibody pair to detect SARS-CoV-2 NP on the protein microarray. Some error bars were not present on the graph because they were too small to be drawn. A single experiment with two technical replicates was performed for each antibody pair.
Graphpad Prism 10, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/graphpad prism 10/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
graphpad prism 10 - by Bioz Stars, 2026-05
90/100 stars
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machine learning toolbox  (MathWorks Inc)
96
MathWorks Inc machine learning toolbox
Representative antibody pair screening experiment. Recombinant SARS-CoV-2 NP was serially diluted and probed on pads. ( a ) A representative protein microarray image showing all 16 pads which are consisted of identical arrays of proteins. The brightness of the image was adjusted from 0 to 15,000 using ImageJ. ( b ) A representative image of a single pad is shown with labels. Each label indicates the following reagents: label 1—fiducials, label 2, 3, 5, and 6—anti-SARS-CoV-2 NP capture antibodies (reagent number 8, 14, 11, and 12 in , respectively), and label 4—recombinant SARS-CoV-2 NP (reagent number 1 in ). The anti-SARS-CoV-2 NP capture antibodies were printed at different concentrations in duplicates. Fluorescence signals captured from the highest printing concentrations (red box) were used from each pad to calculate K D and Bmax. ( c ) A representative graph after curve-fitting using the antibody pair 1 in is shown. Recombinant SARS-CoV-2 NP was serially diluted in a five-fold and probed on protein microarray. A total of ten concentration points were tested. After probing, fluorescence signals from each concentration point were measured and recorded. Using a built-in function in the <t>PRISM</t> <t>software</t> (nonlinear regression, one site—specific binding), K D and Bmax were calculated, which were used for the main parameters to determine the best antibody pair to detect SARS-CoV-2 NP on the protein microarray. Some error bars were not present on the graph because they were too small to be drawn. A single experiment with two technical replicates was performed for each antibody pair.
Machine Learning Toolbox, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/machine learning toolbox/product/MathWorks Inc
Average 96 stars, based on 1 article reviews
machine learning toolbox - by Bioz Stars, 2026-05
96/100 stars
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graphpad prism 5  (GraphPad Software Inc)
90
GraphPad Software Inc graphpad prism 5
Representative antibody pair screening experiment. Recombinant SARS-CoV-2 NP was serially diluted and probed on pads. ( a ) A representative protein microarray image showing all 16 pads which are consisted of identical arrays of proteins. The brightness of the image was adjusted from 0 to 15,000 using ImageJ. ( b ) A representative image of a single pad is shown with labels. Each label indicates the following reagents: label 1—fiducials, label 2, 3, 5, and 6—anti-SARS-CoV-2 NP capture antibodies (reagent number 8, 14, 11, and 12 in , respectively), and label 4—recombinant SARS-CoV-2 NP (reagent number 1 in ). The anti-SARS-CoV-2 NP capture antibodies were printed at different concentrations in duplicates. Fluorescence signals captured from the highest printing concentrations (red box) were used from each pad to calculate K D and Bmax. ( c ) A representative graph after curve-fitting using the antibody pair 1 in is shown. Recombinant SARS-CoV-2 NP was serially diluted in a five-fold and probed on protein microarray. A total of ten concentration points were tested. After probing, fluorescence signals from each concentration point were measured and recorded. Using a built-in function in the <t>PRISM</t> <t>software</t> (nonlinear regression, one site—specific binding), K D and Bmax were calculated, which were used for the main parameters to determine the best antibody pair to detect SARS-CoV-2 NP on the protein microarray. Some error bars were not present on the graph because they were too small to be drawn. A single experiment with two technical replicates was performed for each antibody pair.
Graphpad Prism 5, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/graphpad prism 5/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
graphpad prism 5 - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


Representative antibody pair screening experiment. Recombinant SARS-CoV-2 NP was serially diluted and probed on pads. ( a ) A representative protein microarray image showing all 16 pads which are consisted of identical arrays of proteins. The brightness of the image was adjusted from 0 to 15,000 using ImageJ. ( b ) A representative image of a single pad is shown with labels. Each label indicates the following reagents: label 1—fiducials, label 2, 3, 5, and 6—anti-SARS-CoV-2 NP capture antibodies (reagent number 8, 14, 11, and 12 in , respectively), and label 4—recombinant SARS-CoV-2 NP (reagent number 1 in ). The anti-SARS-CoV-2 NP capture antibodies were printed at different concentrations in duplicates. Fluorescence signals captured from the highest printing concentrations (red box) were used from each pad to calculate K D and Bmax. ( c ) A representative graph after curve-fitting using the antibody pair 1 in is shown. Recombinant SARS-CoV-2 NP was serially diluted in a five-fold and probed on protein microarray. A total of ten concentration points were tested. After probing, fluorescence signals from each concentration point were measured and recorded. Using a built-in function in the PRISM software (nonlinear regression, one site—specific binding), K D and Bmax were calculated, which were used for the main parameters to determine the best antibody pair to detect SARS-CoV-2 NP on the protein microarray. Some error bars were not present on the graph because they were too small to be drawn. A single experiment with two technical replicates was performed for each antibody pair.

Journal: Biomedicines

Article Title: A Protein Microarray-Based Respiratory Viral Antigen Testing Platform for COVID-19 Surveillance

doi: 10.3390/biomedicines10092238

Figure Lengend Snippet: Representative antibody pair screening experiment. Recombinant SARS-CoV-2 NP was serially diluted and probed on pads. ( a ) A representative protein microarray image showing all 16 pads which are consisted of identical arrays of proteins. The brightness of the image was adjusted from 0 to 15,000 using ImageJ. ( b ) A representative image of a single pad is shown with labels. Each label indicates the following reagents: label 1—fiducials, label 2, 3, 5, and 6—anti-SARS-CoV-2 NP capture antibodies (reagent number 8, 14, 11, and 12 in , respectively), and label 4—recombinant SARS-CoV-2 NP (reagent number 1 in ). The anti-SARS-CoV-2 NP capture antibodies were printed at different concentrations in duplicates. Fluorescence signals captured from the highest printing concentrations (red box) were used from each pad to calculate K D and Bmax. ( c ) A representative graph after curve-fitting using the antibody pair 1 in is shown. Recombinant SARS-CoV-2 NP was serially diluted in a five-fold and probed on protein microarray. A total of ten concentration points were tested. After probing, fluorescence signals from each concentration point were measured and recorded. Using a built-in function in the PRISM software (nonlinear regression, one site—specific binding), K D and Bmax were calculated, which were used for the main parameters to determine the best antibody pair to detect SARS-CoV-2 NP on the protein microarray. Some error bars were not present on the graph because they were too small to be drawn. A single experiment with two technical replicates was performed for each antibody pair.

Article Snippet: One-way ANOVA, followed by Tukey’s multiple comparisons, was performed using the built-in function in PRISM software (GraphPad, San Diego, CA, USA).

Techniques: Recombinant, Microarray, Fluorescence, Concentration Assay, Software, Binding Assay