Journal: Journal of neuroinflammation

Article Title: Bruton's tyrosine kinase inhibition ameliorated neuroinflammation during chronic white matter ischemia.

doi: 10.1186/s12974-024-03187-4

Figure Lengend Snippet: Fig. 1 Ischemic demyelination and activation of BTK in microglia induced by BCAS. Ischemic demyelination and activation of BTK in microglia induced by BCAS. (A) The degree of white matter damage and the relative intensity of MBP in the CC at different time points after BCAS. Scale bar = 200 μm. (B) Sum marized data display the severity of CC myelin damage. One-way ANOVA and Tukey post hoc test, n = 6 mice/ group (biological replicates). (C) Proportions of various immune cells in BCAS 1 m brains. (D) Representative images of microglia immunofluorescence, skeletonized microglia, and the expression of BTK and p-BTK in microglia at several time points post BCAS. Scale bar = 10 μm. (E) Quantification analysis of the number, soma area, solidity, round of microglia and the percentage of BTK+microglia and p-BTK+microglia. One-way ANOVA and Tukey post hoc test, n = 6 mice/ group (biological replicates)

Article Snippet: Briefly, the concentration of extracted protein was quantified using BCA Protein Assay Kit (Promoter), then transferred to Nitrocellulose (NC, Satrorius) membranes and blocked with 5% BSA for 1 h, followed by incubated with primary antibodies including BTK (1:1000, 8647, CST) and pBTK (1:1000, NBP178295, NOVUS) overnight at 4 °C.

Techniques: Activation Assay, Immunofluorescence, Expressing

Journal: Pharmacology Research & Perspectives

Article Title: ZYBT1, a potent, irreversible Bruton’s Tyrosine Kinase (BTK) inhibitor that inhibits the C481S BTK with profound efficacy against arthritis and cancer

doi: 10.1002/prp2.565

Figure Lengend Snippet: ZTBT1 inhibited the phosphorylation of (A) BTK (Y223) and (B) PLCγ2 (Y1217). The IC 50 values are summarized in the following table. (C) ZYBT1 could also suppress the secretion of TNFα (filled circle), IL‐6 (square), and IL‐8 (open circle) in a cell based assay. The IC 50 values are shown the attached table. The data presented are representative of three independent experiments

Article Snippet: Human phospho‐BTK (Y223) antibody and human BTK antibody were from R&D system.

Techniques: Phospho-proteomics, Cell Based Assay