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anti pten  (Bioss)
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Bioss anti pten
<t>PTEN</t> is directly suppressed by miR-301a. (a) qRT-PCR verified the PTEN expression in a separate random cohort of cervical carcinoma tissues compared to paired non-carcinoma tissues ( n = 12). Bars represent the mean ± SE, * P < 0.05. (b) Correlation analysis of the relative expression of miR-301a and PTEN in cervical carcinoma tissues (Pearson correlation coefficient = −0.6513). (c, d) miR-301a and PTEN expression levels were determined by qRT-PCR in HeLa cells transfected with miR-Ctl or miR-301a mimic. Bars represent the mean ± SE, * P < 0.05 vs HeLa cells; * P < 0.05 vs miR-Ctl. (e) Western blotting showed PTEN protein level after miR-301a or miR-Ctl overexpression in HeLa cells. The blotting bands were quantified by ImageJ. The relative PTEN expression levels were normalized <t>to</t> <t>GAPDH.</t> Results are mean ± SE ( n = 3). * P < 0.05 vs miR-Ctl. (f) 3′-UTR luciferase reporter assay was performed to confirm that miR-301a bound to specific regions in the PTEN 3′-UTR. Bars represent the mean ± SE, * P < 0.05 vs HeLa cells; * P < 0.05 vs miR-Ctl.
Anti Pten, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PTEN is directly suppressed by miR-301a. (a) qRT-PCR verified the PTEN expression in a separate random cohort of cervical carcinoma tissues compared to paired non-carcinoma tissues ( n = 12). Bars represent the mean ± SE, * P < 0.05. (b) Correlation analysis of the relative expression of miR-301a and PTEN in cervical carcinoma tissues (Pearson correlation coefficient = −0.6513). (c, d) miR-301a and PTEN expression levels were determined by qRT-PCR in HeLa cells transfected with miR-Ctl or miR-301a mimic. Bars represent the mean ± SE, * P < 0.05 vs HeLa cells; * P < 0.05 vs miR-Ctl. (e) Western blotting showed PTEN protein level after miR-301a or miR-Ctl overexpression in HeLa cells. The blotting bands were quantified by ImageJ. The relative PTEN expression levels were normalized to GAPDH. Results are mean ± SE ( n = 3). * P < 0.05 vs miR-Ctl. (f) 3′-UTR luciferase reporter assay was performed to confirm that miR-301a bound to specific regions in the PTEN 3′-UTR. Bars represent the mean ± SE, * P < 0.05 vs HeLa cells; * P < 0.05 vs miR-Ctl.

Journal: Innate Immunity

Article Title: Effect of miR-301a/PTEN pathway on the proliferation and apoptosis of cervical cancer

doi: 10.1177/1753425919840702

Figure Lengend Snippet: PTEN is directly suppressed by miR-301a. (a) qRT-PCR verified the PTEN expression in a separate random cohort of cervical carcinoma tissues compared to paired non-carcinoma tissues ( n = 12). Bars represent the mean ± SE, * P < 0.05. (b) Correlation analysis of the relative expression of miR-301a and PTEN in cervical carcinoma tissues (Pearson correlation coefficient = −0.6513). (c, d) miR-301a and PTEN expression levels were determined by qRT-PCR in HeLa cells transfected with miR-Ctl or miR-301a mimic. Bars represent the mean ± SE, * P < 0.05 vs HeLa cells; * P < 0.05 vs miR-Ctl. (e) Western blotting showed PTEN protein level after miR-301a or miR-Ctl overexpression in HeLa cells. The blotting bands were quantified by ImageJ. The relative PTEN expression levels were normalized to GAPDH. Results are mean ± SE ( n = 3). * P < 0.05 vs miR-Ctl. (f) 3′-UTR luciferase reporter assay was performed to confirm that miR-301a bound to specific regions in the PTEN 3′-UTR. Bars represent the mean ± SE, * P < 0.05 vs HeLa cells; * P < 0.05 vs miR-Ctl.

Article Snippet: As primary Abs were used: anti-PTEN at 1:500 dilution (1:500; bsm-52192R, Bioss, Woburn, MA) and anti-GAPDH (1:500; G9545 Sigma-Aldrich).

Techniques: Quantitative RT-PCR, Expressing, Transfection, Western Blot, Over Expression, Luciferase, Reporter Assay