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Journal: Nature Communications
Article Title: Noncanonical calcium-independent TRPM4 activation governs intestinal fluid homeostasis
doi: 10.1038/s41467-025-68014-7
Figure Lengend Snippet: A Fluorescence images stained with Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4, green) and intestinal epithelial maker, Cytokeratin-18 (CK-18, orange) in colon sections derived from wild-type (WT) (upper panel) and Trpm4 -/- (lower panel) mice. Hoechst staining (blue) was used to visualize the nuclei. Scale bar = 50 µm. B Procedure to investigate the laxative effect of DAB in mice. Fecal water content ( C ) and Bristol score ( D ) of the stool collected from WT and Trpm4 -/- mice between 24 and 36 h after Veh or DAB exposure (n = 7 mice/group; four male and three female). Data are presented as Mean ± SEM. **, p < 0.0001, vs . WT-Veh; ## , p < 0.0001, vs . WT-DAB. Fecal water content ( E ) and Bristol score ( F ) of the stool collected from Trpm4 flox/flox and Trpm ΔIEC mice (n = 7 mice/group; four male and three female). **, p < 0.0001, vs . Trpm4 flox/flox -Veh; ## , p < 0.0001, vs . Trpm4 flox/flox -DAB. Two-way ANOVA followed by Bonferroni’s multiple comparisons test. Figure 1B was Created in BioRender. https://BioRender.com/8h3ia6w .
Article Snippet:
Techniques: Fluorescence, Staining, Derivative Assay