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  • 99
    New England Biolabs bsli
    Representative PAGE picture of RFLP results for TLR9 C296T/ Pro99Leu polymorphism on 12% polyacrylamide gel. Lane M is 100 bp molecular marker, Lane 1 is undigested <t>PCR</t> product and Lanes 2 to 6 are showing digested PCR products of 166 bp and 136 bp (35 bp band is not visible) by <t>BslI</t> enzyme representing CC genotype. (Abbreviations: PAGE, Polyacrylamide Gel Electrophoresis; RFLP, Restriction Fragment Length Polymorphism).
    Bsli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsli/product/New England Biolabs
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    99
    Thermo Fisher bseli enzyme
    Representative PAGE picture of RFLP results for TLR9 C296T/ Pro99Leu polymorphism on 12% polyacrylamide gel. Lane M is 100 bp molecular marker, Lane 1 is undigested <t>PCR</t> product and Lanes 2 to 6 are showing digested PCR products of 166 bp and 136 bp (35 bp band is not visible) by <t>BslI</t> enzyme representing CC genotype. (Abbreviations: PAGE, Polyacrylamide Gel Electrophoresis; RFLP, Restriction Fragment Length Polymorphism).
    Bseli Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bseli enzyme/product/Thermo Fisher
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    94
    Thermo Fisher bsli
    Representative PAGE picture of RFLP results for TLR9 C296T/ Pro99Leu polymorphism on 12% polyacrylamide gel. Lane M is 100 bp molecular marker, Lane 1 is undigested <t>PCR</t> product and Lanes 2 to 6 are showing digested PCR products of 166 bp and 136 bp (35 bp band is not visible) by <t>BslI</t> enzyme representing CC genotype. (Abbreviations: PAGE, Polyacrylamide Gel Electrophoresis; RFLP, Restriction Fragment Length Polymorphism).
    Bsli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher fastdigest bsli
    Representative PAGE picture of RFLP results for TLR9 C296T/ Pro99Leu polymorphism on 12% polyacrylamide gel. Lane M is 100 bp molecular marker, Lane 1 is undigested <t>PCR</t> product and Lanes 2 to 6 are showing digested PCR products of 166 bp and 136 bp (35 bp band is not visible) by <t>BslI</t> enzyme representing CC genotype. (Abbreviations: PAGE, Polyacrylamide Gel Electrophoresis; RFLP, Restriction Fragment Length Polymorphism).
    Fastdigest Bsli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher bsli restriction endonucleases
    Representative PAGE picture of RFLP results for TLR9 C296T/ Pro99Leu polymorphism on 12% polyacrylamide gel. Lane M is 100 bp molecular marker, Lane 1 is undigested <t>PCR</t> product and Lanes 2 to 6 are showing digested PCR products of 166 bp and 136 bp (35 bp band is not visible) by <t>BslI</t> enzyme representing CC genotype. (Abbreviations: PAGE, Polyacrylamide Gel Electrophoresis; RFLP, Restriction Fragment Length Polymorphism).
    Bsli Restriction Endonucleases, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore bandeiraea simplifonica lectin i bsli
    Representative PAGE picture of RFLP results for TLR9 C296T/ Pro99Leu polymorphism on 12% polyacrylamide gel. Lane M is 100 bp molecular marker, Lane 1 is undigested <t>PCR</t> product and Lanes 2 to 6 are showing digested PCR products of 166 bp and 136 bp (35 bp band is not visible) by <t>BslI</t> enzyme representing CC genotype. (Abbreviations: PAGE, Polyacrylamide Gel Electrophoresis; RFLP, Restriction Fragment Length Polymorphism).
    Bandeiraea Simplifonica Lectin I Bsli, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher alexa flour 568 labeled gsli bsli
    Representative PAGE picture of RFLP results for TLR9 C296T/ Pro99Leu polymorphism on 12% polyacrylamide gel. Lane M is 100 bp molecular marker, Lane 1 is undigested <t>PCR</t> product and Lanes 2 to 6 are showing digested PCR products of 166 bp and 136 bp (35 bp band is not visible) by <t>BslI</t> enzyme representing CC genotype. (Abbreviations: PAGE, Polyacrylamide Gel Electrophoresis; RFLP, Restriction Fragment Length Polymorphism).
    Alexa Flour 568 Labeled Gsli Bsli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative PAGE picture of RFLP results for TLR9 C296T/ Pro99Leu polymorphism on 12% polyacrylamide gel. Lane M is 100 bp molecular marker, Lane 1 is undigested PCR product and Lanes 2 to 6 are showing digested PCR products of 166 bp and 136 bp (35 bp band is not visible) by BslI enzyme representing CC genotype. (Abbreviations: PAGE, Polyacrylamide Gel Electrophoresis; RFLP, Restriction Fragment Length Polymorphism).

    Journal: F1000Research

    Article Title: Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer

    doi: 10.12688/f1000research.14840.2

    Figure Lengend Snippet: Representative PAGE picture of RFLP results for TLR9 C296T/ Pro99Leu polymorphism on 12% polyacrylamide gel. Lane M is 100 bp molecular marker, Lane 1 is undigested PCR product and Lanes 2 to 6 are showing digested PCR products of 166 bp and 136 bp (35 bp band is not visible) by BslI enzyme representing CC genotype. (Abbreviations: PAGE, Polyacrylamide Gel Electrophoresis; RFLP, Restriction Fragment Length Polymorphism).

    Article Snippet: Upon confirmation of 337 bp PCR product on 2% ethidium bromide-stained agarose gel, 10µl PCR product was digested with BslI restriction enzyme (New England Biolabs, USA; Cat# R0555S) at 55°C overnight, separated on 12% polyacrylamide gel and analysed on a GelDoc system (BioRad, USA) for genotype identification.

    Techniques: Polyacrylamide Gel Electrophoresis, Marker, Polymerase Chain Reaction

    Methylation-sensitive endonuclease digested PCR and Southern analysis of ERF6 , SUR4 , and KCS13 upstream regions over one year. (A) Methylation-sensitive endonuclease digested PCR amplification of ERF6 upstream region. Top: schematic diagram of the identification of a methylation-sensitive BstX I digenstion site (CCANNNNNNTGG) at −275 bp of the ERF6 promoter. The bold C indicates a CHH site with annual methylation pattern change, corresponding to the cytosine labelled with red triangles in Figure 3A . Bottom: PCR amplification using genomic DNA with (+) or without (−) BstX I digestion. (B) Southern blot of genomic DNA harvested at different times of the year, first digested by a methylation non-sensitive endonuclease Mbo II (TCTTC) to obtain a full length fragment of 605 bp from −621 to −15 of ERF6 upstream regions, then digested thoroughly with BstX I, and probed with the fragment from −263 to −21 nt. The signal intensities of the band of BstX I-cleaved 244 bp changed at different time-of-year (see Table S8 ), indicating the methlytion levels of this CHH site were different, consistent with the bisulfite sequencing data in Figure 3A and methylation-sensitive endonuclease digested PCR results in Figure 4A . The same methylation-sensitive endonuclease digested PCR experiments were performed for the upstream regions of SUR4 (C) and KCS13 (E), except the methylation-sensitive endonucleases used were HinF I and Bsl I, respectively. Further, the same methylation-sensitive endonuclease digested Southern experiments were performed for the upstream regions of SUR4 (D) and KCS13 (F), except the genomic DNA were first digested by Bcl I (TGATCA) and NSi I (ATGCAT), then digested by methylation-sensitive endonucleases HinF I (GANTC) and Bsl I (CCNNNNNNNGG), respectively. The signal intensities of HinF I- and Bsl I-cleaved 330 bp and 837 bp changed similarly (see Table S8 ).

    Journal: PLoS ONE

    Article Title: A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns

    doi: 10.1371/journal.pone.0060547

    Figure Lengend Snippet: Methylation-sensitive endonuclease digested PCR and Southern analysis of ERF6 , SUR4 , and KCS13 upstream regions over one year. (A) Methylation-sensitive endonuclease digested PCR amplification of ERF6 upstream region. Top: schematic diagram of the identification of a methylation-sensitive BstX I digenstion site (CCANNNNNNTGG) at −275 bp of the ERF6 promoter. The bold C indicates a CHH site with annual methylation pattern change, corresponding to the cytosine labelled with red triangles in Figure 3A . Bottom: PCR amplification using genomic DNA with (+) or without (−) BstX I digestion. (B) Southern blot of genomic DNA harvested at different times of the year, first digested by a methylation non-sensitive endonuclease Mbo II (TCTTC) to obtain a full length fragment of 605 bp from −621 to −15 of ERF6 upstream regions, then digested thoroughly with BstX I, and probed with the fragment from −263 to −21 nt. The signal intensities of the band of BstX I-cleaved 244 bp changed at different time-of-year (see Table S8 ), indicating the methlytion levels of this CHH site were different, consistent with the bisulfite sequencing data in Figure 3A and methylation-sensitive endonuclease digested PCR results in Figure 4A . The same methylation-sensitive endonuclease digested PCR experiments were performed for the upstream regions of SUR4 (C) and KCS13 (E), except the methylation-sensitive endonucleases used were HinF I and Bsl I, respectively. Further, the same methylation-sensitive endonuclease digested Southern experiments were performed for the upstream regions of SUR4 (D) and KCS13 (F), except the genomic DNA were first digested by Bcl I (TGATCA) and NSi I (ATGCAT), then digested by methylation-sensitive endonucleases HinF I (GANTC) and Bsl I (CCNNNNNNNGG), respectively. The signal intensities of HinF I- and Bsl I-cleaved 330 bp and 837 bp changed similarly (see Table S8 ).

    Article Snippet: Aliquots (1 µg) were digested by incubation with 1 µl Bsl I (10,000 U/ml) in NEBuffer 3 at 55°C for 16 h, with 1 µl HinF I (10,000 U/ml) in NEBuffer 4 at 37°C for 16 h, or with 1 µl BstX I (10,000 U/ml) in NEBuffer 3 at 37°C for 16 h. One twentieth of the digested DNA sample was used as the template for each PCR analysis.

    Techniques: Methylation, Polymerase Chain Reaction, Amplification, Southern Blot, Methylation Sequencing

    Methylation-sensitive endonuclease digested PCR and Southern analysis of ERF6 , SUR4 , and KCS13 upstream regions in ROS1 RNAi lines. (A) Analysis of relative ERF6 transcription in ovules from ROS1 RNAi lines by qRT-PCR. The level of ERF6 transcripts in ovules from the empty vector line (V) was arbitrarily defined as 1. (B) Southern blot analysis of genomic DNA prepared from ROS1 RNAi lines digested thoroughly with BstX I. See detailed information in Figure 4 legend. Similar qRT-PCR experiments were performed for SUR4 (C) and KCS13 (E) transcriptions, as well as similar Southern experiments for SUR4 (D) and KCS13 (F), respectively. Note the reduced intensities of the BstX I-, HinF I- and Bsl I-cleaved bands in all three RNAi lines compared to the vector line.

    Journal: PLoS ONE

    Article Title: A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns

    doi: 10.1371/journal.pone.0060547

    Figure Lengend Snippet: Methylation-sensitive endonuclease digested PCR and Southern analysis of ERF6 , SUR4 , and KCS13 upstream regions in ROS1 RNAi lines. (A) Analysis of relative ERF6 transcription in ovules from ROS1 RNAi lines by qRT-PCR. The level of ERF6 transcripts in ovules from the empty vector line (V) was arbitrarily defined as 1. (B) Southern blot analysis of genomic DNA prepared from ROS1 RNAi lines digested thoroughly with BstX I. See detailed information in Figure 4 legend. Similar qRT-PCR experiments were performed for SUR4 (C) and KCS13 (E) transcriptions, as well as similar Southern experiments for SUR4 (D) and KCS13 (F), respectively. Note the reduced intensities of the BstX I-, HinF I- and Bsl I-cleaved bands in all three RNAi lines compared to the vector line.

    Article Snippet: Aliquots (1 µg) were digested by incubation with 1 µl Bsl I (10,000 U/ml) in NEBuffer 3 at 55°C for 16 h, with 1 µl HinF I (10,000 U/ml) in NEBuffer 4 at 37°C for 16 h, or with 1 µl BstX I (10,000 U/ml) in NEBuffer 3 at 37°C for 16 h. One twentieth of the digested DNA sample was used as the template for each PCR analysis.

    Techniques: Methylation, Polymerase Chain Reaction, Quantitative RT-PCR, Plasmid Preparation, Southern Blot