Journal: American Journal of Physiology - Endocrinology and Metabolism

Article Title: Propionate-induced changes in cardiac metabolism, notably CoA trapping, are not altered by l-carnitine

doi: 10.1152/ajpendo.00081.2018

Figure Lengend Snippet: Short-chain dicarboxylylcarnitines have little 13 C labeling in the perfused hearts with [ 13 C 6 ]glucose and [1- 13 C]palmitate. Rat hearts were perfused with 40% 11 mM [ 13 C 6 ]glucose, [1- 13 C]palmitate, 0.1 mM 2-DG, 3% BSA, 100 µU insulin, and 50 µM

Article Snippet: The perfusions were conducted as the following groups: 1 ) hearts perfused with 11 mM 40% [13 C6 ]glucose, 0.4 mM [1-13 C]palmitate, 3% BSA (fatty acid free; Fisher Scientific), 100 µU/ml insulin, 50 µM l -carnitine, and 0.1 mM 2-DG ± (3 mM propionate ± 3 mM l -carnitine); 2 ) hearts perfused with 11 mM glucose, 0.4 mM palmitate, 3% BSA, 100 µU/ml insulin, 50 µM l -carnitine, and 3 mM [13 C3 ]propionate; 3 ) hearts perfused with 11 mM glucose, 100 µU/ml insulin, 50 µM l -carnitine, 3% BSA, and 0.2 mM [1-13 C]octanoate ± 3 mM propionate.

Techniques: Labeling

Journal: American Journal of Physiology - Endocrinology and Metabolism

Article Title: Propionate-induced changes in cardiac metabolism, notably CoA trapping, are not altered by l-carnitine

doi: 10.1152/ajpendo.00081.2018

Figure Lengend Snippet: Propionate-mediated changes of acyl-CoAs and acylcarnitines in the perfused hearts. Rat hearts were perfused with 40% 11 mM [ 13 C 6 ]glucose, [1- 13 C]palmitate, 0.1 mM 2-DG, 3% BSA, 100 µU insulin, and 50 µM l -carnitine (control, n = 5), control

Article Snippet: The perfusions were conducted as the following groups: 1 ) hearts perfused with 11 mM 40% [13 C6 ]glucose, 0.4 mM [1-13 C]palmitate, 3% BSA (fatty acid free; Fisher Scientific), 100 µU/ml insulin, 50 µM l -carnitine, and 0.1 mM 2-DG ± (3 mM propionate ± 3 mM l -carnitine); 2 ) hearts perfused with 11 mM glucose, 0.4 mM palmitate, 3% BSA, 100 µU/ml insulin, 50 µM l -carnitine, and 3 mM [13 C3 ]propionate; 3 ) hearts perfused with 11 mM glucose, 100 µU/ml insulin, 50 µM l -carnitine, 3% BSA, and 0.2 mM [1-13 C]octanoate ± 3 mM propionate.

Techniques:

Journal: American Journal of Physiology - Endocrinology and Metabolism

Article Title: Propionate-induced changes in cardiac metabolism, notably CoA trapping, are not altered by l-carnitine

doi: 10.1152/ajpendo.00081.2018

Figure Lengend Snippet: M3 acylcarnitine synthesis kinetics in the heart perfused with [ 2 H 3 ] l -carnitine. Rat hearts were perfused with 11 mM glucose, palmitate, 3% BSA, 100 µU insulin, and 50 µM [ 2 H 3 ] l -carnitine for 0, 2, 5, 10, 20, 40, and 60 min. There was

Article Snippet: The perfusions were conducted as the following groups: 1 ) hearts perfused with 11 mM 40% [13 C6 ]glucose, 0.4 mM [1-13 C]palmitate, 3% BSA (fatty acid free; Fisher Scientific), 100 µU/ml insulin, 50 µM l -carnitine, and 0.1 mM 2-DG ± (3 mM propionate ± 3 mM l -carnitine); 2 ) hearts perfused with 11 mM glucose, 0.4 mM palmitate, 3% BSA, 100 µU/ml insulin, 50 µM l -carnitine, and 3 mM [13 C3 ]propionate; 3 ) hearts perfused with 11 mM glucose, 100 µU/ml insulin, 50 µM l -carnitine, 3% BSA, and 0.2 mM [1-13 C]octanoate ± 3 mM propionate.

Techniques:

Journal: American Journal of Physiology - Endocrinology and Metabolism

Article Title: Propionate-induced changes in cardiac metabolism, notably CoA trapping, are not altered by l-carnitine

doi: 10.1152/ajpendo.00081.2018

Figure Lengend Snippet: The labeling of metabolites from [ 13 C 3 ]propionate in the perfused hearts and livers. Rat hearts were perfused with 11 mM glucose, palmitate, 3% BSA, 100 µU insulin, 3 mM [ 13 C 3 ]propionate, and 50 µM l -carnitine ( n = 6). Liver perfusion

Article Snippet: The perfusions were conducted as the following groups: 1 ) hearts perfused with 11 mM 40% [13 C6 ]glucose, 0.4 mM [1-13 C]palmitate, 3% BSA (fatty acid free; Fisher Scientific), 100 µU/ml insulin, 50 µM l -carnitine, and 0.1 mM 2-DG ± (3 mM propionate ± 3 mM l -carnitine); 2 ) hearts perfused with 11 mM glucose, 0.4 mM palmitate, 3% BSA, 100 µU/ml insulin, 50 µM l -carnitine, and 3 mM [13 C3 ]propionate; 3 ) hearts perfused with 11 mM glucose, 100 µU/ml insulin, 50 µM l -carnitine, 3% BSA, and 0.2 mM [1-13 C]octanoate ± 3 mM propionate.

Techniques: Labeling

Journal: American Journal of Physiology - Endocrinology and Metabolism

Article Title: Propionate-induced changes in cardiac metabolism, notably CoA trapping, are not altered by l-carnitine

doi: 10.1152/ajpendo.00081.2018

Figure Lengend Snippet: Short-chain dicarboxylylcarnitines are more labeled from [ 13 C 3 ]propionate in liver than in heart. Rat hearts were perfused with 11 mM glucose, palmitate, 3% BSA, 100 µU insulin, 50 µM l -carnitine, and 3 mM [ 13 C 3 ]propionate for 25 min.

Article Snippet: The perfusions were conducted as the following groups: 1 ) hearts perfused with 11 mM 40% [13 C6 ]glucose, 0.4 mM [1-13 C]palmitate, 3% BSA (fatty acid free; Fisher Scientific), 100 µU/ml insulin, 50 µM l -carnitine, and 0.1 mM 2-DG ± (3 mM propionate ± 3 mM l -carnitine); 2 ) hearts perfused with 11 mM glucose, 0.4 mM palmitate, 3% BSA, 100 µU/ml insulin, 50 µM l -carnitine, and 3 mM [13 C3 ]propionate; 3 ) hearts perfused with 11 mM glucose, 100 µU/ml insulin, 50 µM l -carnitine, 3% BSA, and 0.2 mM [1-13 C]octanoate ± 3 mM propionate.

Techniques: Labeling

Journal: American Journal of Physiology - Endocrinology and Metabolism

Article Title: Propionate-induced changes in cardiac metabolism, notably CoA trapping, are not altered by l-carnitine

doi: 10.1152/ajpendo.00081.2018

Figure Lengend Snippet: Metabolites altered by propionate. Rat hearts were perfused with 40% 11 mM [ 13 C 6 ]glucose, [1- 13 C]palmitate, 0.1 mM 2-DG, 3% BSA, 100 µU insulin, and 50 µM l -carnitine (control, n = 5), control + 3 mM propionate (PA, n = 5), and PA + 3

Article Snippet: The perfusions were conducted as the following groups: 1 ) hearts perfused with 11 mM 40% [13 C6 ]glucose, 0.4 mM [1-13 C]palmitate, 3% BSA (fatty acid free; Fisher Scientific), 100 µU/ml insulin, 50 µM l -carnitine, and 0.1 mM 2-DG ± (3 mM propionate ± 3 mM l -carnitine); 2 ) hearts perfused with 11 mM glucose, 0.4 mM palmitate, 3% BSA, 100 µU/ml insulin, 50 µM l -carnitine, and 3 mM [13 C3 ]propionate; 3 ) hearts perfused with 11 mM glucose, 100 µU/ml insulin, 50 µM l -carnitine, 3% BSA, and 0.2 mM [1-13 C]octanoate ± 3 mM propionate.

Techniques:

Journal: American Journal of Physiology - Endocrinology and Metabolism

Article Title: Propionate-induced changes in cardiac metabolism, notably CoA trapping, are not altered by l-carnitine

doi: 10.1152/ajpendo.00081.2018

Figure Lengend Snippet: Propionate suppressed mitochondrial metabolism of octanoate to TCA. Rat hearts were perfused in Langendorff mode with 11 mM glucose, 0.2 mM [1- 13 C]octanoate, 3% BSA, 100 µU insulin, and 50 µM l -carnitine (M1 OA, n = 4), control + 3 mM

Article Snippet: The perfusions were conducted as the following groups: 1 ) hearts perfused with 11 mM 40% [13 C6 ]glucose, 0.4 mM [1-13 C]palmitate, 3% BSA (fatty acid free; Fisher Scientific), 100 µU/ml insulin, 50 µM l -carnitine, and 0.1 mM 2-DG ± (3 mM propionate ± 3 mM l -carnitine); 2 ) hearts perfused with 11 mM glucose, 0.4 mM palmitate, 3% BSA, 100 µU/ml insulin, 50 µM l -carnitine, and 3 mM [13 C3 ]propionate; 3 ) hearts perfused with 11 mM glucose, 100 µU/ml insulin, 50 µM l -carnitine, 3% BSA, and 0.2 mM [1-13 C]octanoate ± 3 mM propionate.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Advanced Glycation End Product-Induced Astrocytic Differentiation of Cultured Neurospheres through Inhibition of Notch-Hes1 Pathway-Mediated Neurogenesis

doi: 10.3390/ijms15010159

Figure Lengend Snippet: AGEs stimulated the differentiation of cultured neurospheres into astrocytes by inhibiting neuronal differention through Notch-Hes1 pathway. ( A ) NSCs derived from SGZ could generate into neurons (TUJ1) and astrocytes (GFAP) after seven days of culture in differentiation medium. Scale bar, 50 μm; and ( B , C ) 400 μg/mL AGE-BSA induced a significant decrease in the proportion of TUJ1 cells and increase in the numbers of GFAP-immunoreactive cells with astrocytic morphology (* p

Article Snippet: AGE-BSA Preparations AGE-BSA was prepared by incubating 5.0 g/L BSA (Amresco, Cleveland, OH, USA) with 0.5 mol/L glucose (Sigma, Oakville, ON, Canada) at 37 °C for 90 days under sterile conditions and finally dialyzed against PBS for 48 h, as previously described [ , ].

Techniques: Cell Culture, Derivative Assay

Journal: International Journal of Molecular Sciences

Article Title: Advanced Glycation End Product-Induced Astrocytic Differentiation of Cultured Neurospheres through Inhibition of Notch-Hes1 Pathway-Mediated Neurogenesis

doi: 10.3390/ijms15010159

Figure Lengend Snippet: AGEs stimulated the protein and gene expression of Notch-Hes1 pathway in cultured neurospheres. ( A ) Western blotting shows that AGE-BSA increased protein expression of NICD and Hes1 in the NSCs (Vector control) after 7 days of culture in differentiation medium (* p

Article Snippet: AGE-BSA Preparations AGE-BSA was prepared by incubating 5.0 g/L BSA (Amresco, Cleveland, OH, USA) with 0.5 mol/L glucose (Sigma, Oakville, ON, Canada) at 37 °C for 90 days under sterile conditions and finally dialyzed against PBS for 48 h, as previously described [ , ].

Techniques: Expressing, Cell Culture, Western Blot, Plasmid Preparation