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  • 99
    Thermo Fisher bsa
    GAL efficiently labels Gal and GalNAc residues on cell surface glycoproteins. ( A ) Asialo-K20 cells were incubated in <t>PBS</t> with 5 mg/mL <t>BSA,</t> pH 6.7, containing 250 μM aminooxy-biotin in the presence or absence of 10 mM aniline and 50 U/mL galactose
    Bsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25730 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore fat free bsa
    GAL efficiently labels Gal and GalNAc residues on cell surface glycoproteins. ( A ) Asialo-K20 cells were incubated in <t>PBS</t> with 5 mg/mL <t>BSA,</t> pH 6.7, containing 250 μM aminooxy-biotin in the presence or absence of 10 mM aniline and 50 U/mL galactose
    Fat Free Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc bovin serum albumin bsa
    <t>ApoA-IV</t> induces NR1D1 expression in primary mouse hepatocytes and HepG2 and HEK-293 cells. The cells were starved in DMEM with 1% <t>BSA</t> overnight and then treated with or without 20 μg/ml r-m-apoA-IV in mouse hepatocytes for the indicated times or 50 μg/ml r-h-apoA-IV in HepG2 and HEK-293 for 2 h. mRNA levels in primary mouse hepatocytes were quantitated by real-time RT-PCR and normalized to cyclophilin. NR1D1 protein levels were detected by Western immunoblotting with anti-NR1D1 antibody and then standardized against actin. The results are from three independent experiments with at least three samples each time. Data are presented as mean ± S.E. *, p
    Bovin Serum Albumin Bsa, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Merck KGaA bovine serum album bsa
    <t>ApoA-IV</t> induces NR1D1 expression in primary mouse hepatocytes and HepG2 and HEK-293 cells. The cells were starved in DMEM with 1% <t>BSA</t> overnight and then treated with or without 20 μg/ml r-m-apoA-IV in mouse hepatocytes for the indicated times or 50 μg/ml r-h-apoA-IV in HepG2 and HEK-293 for 2 h. mRNA levels in primary mouse hepatocytes were quantitated by real-time RT-PCR and normalized to cyclophilin. NR1D1 protein levels were detected by Western immunoblotting with anti-NR1D1 antibody and then standardized against actin. The results are from three independent experiments with at least three samples each time. Data are presented as mean ± S.E. *, p
    Bovine Serum Album Bsa, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific bsa
    IL-6 has no effect on the phagocytic capacity of IgM + B cells. Splenocytes were cultured in the presence of IL-6 (200 ng/ml) or LPS (100 μg/ml) for 24, 48 or 72 h at 20 °C. Non-stimulated controls were also included. After the different incubation periods, cells were exposed to fluorescent beads for a further 3 h at 20 °C. Non-ingested beads were removed by centrifugation over a cushion of 3% (w/v) <t>BSA</t> in <t>PBS</t> supplemented with 4.5 (w/v) D-glucose (Sigma). ( a ) Data are shown as mean percentage of phagocytic IgM + B cells (left) or Mean fluorescence intensity (MFI) (right) + standard deviation from six independent fish. * P
    Bsa, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bsa  (Amresco)
    92
    Amresco bsa
    In vitro release of isoniazid <t>(INH:</t> ) and rifampicin (RFP: ) from INH and RFP-loaded bovine serum albumin nanoparticles <t>(INH-RFP-BSA-NPs)</t> into culture medium.
    Bsa, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 555 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    STEMCELL Technologies Inc bsa
    In vitro release of isoniazid <t>(INH:</t> ) and rifampicin (RFP: ) from INH and RFP-loaded bovine serum albumin nanoparticles <t>(INH-RFP-BSA-NPs)</t> into culture medium.
    Bsa, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 92/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bsa  (Bio-Rad)
    93
    Bio-Rad bsa
    Detection of <t>BamHI/DNA</t> interaction by mwPIFE. ( A ) Scheme of DNA probes for detecting BamHI binding. Position of the BamHI restriction site is indicated. ( B ) Normalized PIFE values obtained with different probes incubated with <t>BSA</t> or BamHI. Error bars indicate standard deviations from measurements of three independent wells. P value was calculated by two-tailed t-test. ( C ) Effect of divalent cations on BamHI binding and cleavage. A 25 bp oligonucleotide with the restriction site 1 bp from Cy3 was used as a probe. Chart shows normalized PIFE values obtained upon addition of BamHI and after the subsequent wash. Error bars indicate standard deviations from measurements of three independent wells.
    Bsa, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 2784 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Beijing Solarbio Science bovine serum album
    Detection of <t>BamHI/DNA</t> interaction by mwPIFE. ( A ) Scheme of DNA probes for detecting BamHI binding. Position of the BamHI restriction site is indicated. ( B ) Normalized PIFE values obtained with different probes incubated with <t>BSA</t> or BamHI. Error bars indicate standard deviations from measurements of three independent wells. P value was calculated by two-tailed t-test. ( C ) Effect of divalent cations on BamHI binding and cleavage. A 25 bp oligonucleotide with the restriction site 1 bp from Cy3 was used as a probe. Chart shows normalized PIFE values obtained upon addition of BamHI and after the subsequent wash. Error bars indicate standard deviations from measurements of three independent wells.
    Bovine Serum Album, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare bsa
    Radioactivity registered after chromatography of serum samples containing the <t>RAM–nanodiamond–BSA</t> I125 complex: 1 —sorbent with immobilized BSA, 2 —sorbent with immobilized mouse <t>IgG</t> (percentage of radioactivity of the initial samples used for chromatography)
    Bsa, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 2411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Miltenyi Biotec bsa
    Radioactivity registered after chromatography of serum samples containing the <t>RAM–nanodiamond–BSA</t> I125 complex: 1 —sorbent with immobilized BSA, 2 —sorbent with immobilized mouse <t>IgG</t> (percentage of radioactivity of the initial samples used for chromatography)
    Bsa, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 888 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher maleimide activated bovine serum album
    Radioactivity registered after chromatography of serum samples containing the <t>RAM–nanodiamond–BSA</t> I125 complex: 1 —sorbent with immobilized BSA, 2 —sorbent with immobilized mouse <t>IgG</t> (percentage of radioactivity of the initial samples used for chromatography)
    Maleimide Activated Bovine Serum Album, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Abcam bovine serum album bsa
    The ARC CARD is required for the ability of ARC to suppress TNF α -induced necrosis. ( a ) Immunoblot showing inhibition of TNF α -induced <t>HMGB1</t> release by wild-type ARC but not by the CARD-defective double-point ARC mutant (L31F; G69R). L929 cells were stably transduced with empty vector (Φ), ARC-HA (ARC) or ARC-HA double-point CARD mutant (DM). Immunblot of bovine serum albumin <t>(BSA)</t> in media used as loading control. ( b ) Inhibition of TNF α -induced LDH release by ARC requires the CARD. Data shown as mean±S.E. from n =4. *** P -value
    Bovine Serum Album Bsa, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore bsa
    TAG-1 and Contactin bind to CD24 and are receptors for Lewis x . A , Lysates from CHO cells transfected with TAX-1 (CHO/TAX-1), Contactin (CHO/Contactin), or NCAM120 (CHO/NCAM120) were incubated with epoxy beads coated with Lewis x <t>–BSA</t> (Le x <t>-BSA),</t> N -acetyllactosamine–BSA (LN-BSA), and BSA. After pull-down, beads were analyzed by Western blotting using antibodies to TAG-1 (lanes 1–4), Contactin (lanes 5–8), or NCAM (lanes 9–12). B , Immunoprecipitates from brain homogenate using CD24 antibody (α-CD24) or control rat IgG (rIgG) were analyzed by Western blotting using antibodies against L1, TAG-1, Contactin, NCAM, or CD24. C , CD24-coupled epoxy beads were incubated with TAX-1-Fc, Contactin-Fc, CHL1-Fc, or Fc alone. After pull-down, beads (PD CD24) were analyzed by Western blotting using HRP-coupled anti-human antibody. D , E , Cerebellar tissue (input) was used for immunoprecipitations with CD24 with antibody (α-CD24), with control rat IgG (rIgG) or without antibody (−) or for immunoprecipitations with TAG-1 antibody (α-TAG-1), Contactin antibody (α-Contactin), or control goat IgG (gIgG) or without antibody (−). Immunoprecipitates were analyzed by Western blotting using antibodies against CD24, TAG-1, Contactin, or CHL1.
    Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 42788 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Boster Bio bsa
    TAG-1 and Contactin bind to CD24 and are receptors for Lewis x . A , Lysates from CHO cells transfected with TAX-1 (CHO/TAX-1), Contactin (CHO/Contactin), or NCAM120 (CHO/NCAM120) were incubated with epoxy beads coated with Lewis x <t>–BSA</t> (Le x <t>-BSA),</t> N -acetyllactosamine–BSA (LN-BSA), and BSA. After pull-down, beads were analyzed by Western blotting using antibodies to TAG-1 (lanes 1–4), Contactin (lanes 5–8), or NCAM (lanes 9–12). B , Immunoprecipitates from brain homogenate using CD24 antibody (α-CD24) or control rat IgG (rIgG) were analyzed by Western blotting using antibodies against L1, TAG-1, Contactin, NCAM, or CD24. C , CD24-coupled epoxy beads were incubated with TAX-1-Fc, Contactin-Fc, CHL1-Fc, or Fc alone. After pull-down, beads (PD CD24) were analyzed by Western blotting using HRP-coupled anti-human antibody. D , E , Cerebellar tissue (input) was used for immunoprecipitations with CD24 with antibody (α-CD24), with control rat IgG (rIgG) or without antibody (−) or for immunoprecipitations with TAG-1 antibody (α-TAG-1), Contactin antibody (α-Contactin), or control goat IgG (gIgG) or without antibody (−). Immunoprecipitates were analyzed by Western blotting using antibodies against CD24, TAG-1, Contactin, or CHL1.
    Bsa, supplied by Boster Bio, used in various techniques. Bioz Stars score: 99/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher bsa acetylated
    Effect of <t>BSA</t> type on the TaqMan-based PCR yield. Acetylated BSA from <t>Ambion</t> and Promega, non-acetylated BSA from Sigma were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).
    Bsa Acetylated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    GAL efficiently labels Gal and GalNAc residues on cell surface glycoproteins. ( A ) Asialo-K20 cells were incubated in PBS with 5 mg/mL BSA, pH 6.7, containing 250 μM aminooxy-biotin in the presence or absence of 10 mM aniline and 50 U/mL galactose

    Journal: Glycobiology

    Article Title: Glycoproteomics enabled by tagging sialic acid- or galactose-terminated glycans

    doi: 10.1093/glycob/cws144

    Figure Lengend Snippet: GAL efficiently labels Gal and GalNAc residues on cell surface glycoproteins. ( A ) Asialo-K20 cells were incubated in PBS with 5 mg/mL BSA, pH 6.7, containing 250 μM aminooxy-biotin in the presence or absence of 10 mM aniline and 50 U/mL galactose

    Article Snippet: For the oxime ligation step, cells were suspended to 1 × 107 cells/mL in PBS/5% BSA, pH 6.7 containing 250 μM aminooxy-biotin, aminooxy-AF488 (Invitrogen Corporation) or aminooxy-FLAG peptide and 10 mM aniline (Sigma–Aldrich) for 90 min at 4°C with end-over-end mixing.

    Techniques: Incubation

    ApoA-IV induces NR1D1 expression in primary mouse hepatocytes and HepG2 and HEK-293 cells. The cells were starved in DMEM with 1% BSA overnight and then treated with or without 20 μg/ml r-m-apoA-IV in mouse hepatocytes for the indicated times or 50 μg/ml r-h-apoA-IV in HepG2 and HEK-293 for 2 h. mRNA levels in primary mouse hepatocytes were quantitated by real-time RT-PCR and normalized to cyclophilin. NR1D1 protein levels were detected by Western immunoblotting with anti-NR1D1 antibody and then standardized against actin. The results are from three independent experiments with at least three samples each time. Data are presented as mean ± S.E. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Apolipoprotein A-IV Reduces Hepatic Gluconeogenesis through Nuclear Receptor NR1D1 *

    doi: 10.1074/jbc.M113.511766

    Figure Lengend Snippet: ApoA-IV induces NR1D1 expression in primary mouse hepatocytes and HepG2 and HEK-293 cells. The cells were starved in DMEM with 1% BSA overnight and then treated with or without 20 μg/ml r-m-apoA-IV in mouse hepatocytes for the indicated times or 50 μg/ml r-h-apoA-IV in HepG2 and HEK-293 for 2 h. mRNA levels in primary mouse hepatocytes were quantitated by real-time RT-PCR and normalized to cyclophilin. NR1D1 protein levels were detected by Western immunoblotting with anti-NR1D1 antibody and then standardized against actin. The results are from three independent experiments with at least three samples each time. Data are presented as mean ± S.E. *, p

    Article Snippet: Cells were seeded on 8-well chamber slides, maintained for 48 h with growth medium, serum-starved overnight in growth medium with 1% BSA, and then incubated with recombinant human apoA-IV protein fused with GFP (r-h-apoA-IV-GFP) or GFP only as a negative control for 2 h. After fixation, permeabilization, and blocking, the cells were incubated with anti-human NR1D1 (1:200) and anti-GFP (1:200) antibodies (Cell Signaling Technology, Beverly, MA) and then with Alexa Fluor 594-conjugated goat anti-rabbit and FITC-conjugated goat anti-mouse secondary antibodies (Invitrogen).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    IL-6 has no effect on the phagocytic capacity of IgM + B cells. Splenocytes were cultured in the presence of IL-6 (200 ng/ml) or LPS (100 μg/ml) for 24, 48 or 72 h at 20 °C. Non-stimulated controls were also included. After the different incubation periods, cells were exposed to fluorescent beads for a further 3 h at 20 °C. Non-ingested beads were removed by centrifugation over a cushion of 3% (w/v) BSA in PBS supplemented with 4.5 (w/v) D-glucose (Sigma). ( a ) Data are shown as mean percentage of phagocytic IgM + B cells (left) or Mean fluorescence intensity (MFI) (right) + standard deviation from six independent fish. * P

    Journal: Scientific Reports

    Article Title: Distinct Differentiation Programs Triggered by IL-6 and LPS in Teleost IgM+ B Cells in The Absence of Germinal Centers

    doi: 10.1038/srep30004

    Figure Lengend Snippet: IL-6 has no effect on the phagocytic capacity of IgM + B cells. Splenocytes were cultured in the presence of IL-6 (200 ng/ml) or LPS (100 μg/ml) for 24, 48 or 72 h at 20 °C. Non-stimulated controls were also included. After the different incubation periods, cells were exposed to fluorescent beads for a further 3 h at 20 °C. Non-ingested beads were removed by centrifugation over a cushion of 3% (w/v) BSA in PBS supplemented with 4.5 (w/v) D-glucose (Sigma). ( a ) Data are shown as mean percentage of phagocytic IgM + B cells (left) or Mean fluorescence intensity (MFI) (right) + standard deviation from six independent fish. * P

    Article Snippet: Non-ingested beads were removed by centrifugation (100 × g for 10 min at 4 °C) over a cushion of 3% (w/v) BSA (Fraction V; Fisher Scientific) in PBS supplemented with 4.5 (w/v) D-glucose (Sigma).

    Techniques: Cell Culture, Incubation, Centrifugation, Fluorescence, Standard Deviation, Fluorescence In Situ Hybridization

    In vitro release of isoniazid (INH: ) and rifampicin (RFP: ) from INH and RFP-loaded bovine serum albumin nanoparticles (INH-RFP-BSA-NPs) into culture medium.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Development and In Vitro Release of Isoniazid and Rifampicin-Loaded Bovine Serum Albumin Nanoparticles

    doi: 10.12659/MSM.905581

    Figure Lengend Snippet: In vitro release of isoniazid (INH: ) and rifampicin (RFP: ) from INH and RFP-loaded bovine serum albumin nanoparticles (INH-RFP-BSA-NPs) into culture medium.

    Article Snippet: Preparation of INH-RFP-BSA-NPs An aqueous phase was prepared by thoroughly dissolving 0.4 g of INH (Wuhan Fuchi Biotechnology Co. Ltd., Wuhan, China) and 1.0 g of BSA (Amresco, Solon, OH, USA) in 200 ml of purified water at room temperature using ultrasound.

    Techniques: In Vitro

    Detection of BamHI/DNA interaction by mwPIFE. ( A ) Scheme of DNA probes for detecting BamHI binding. Position of the BamHI restriction site is indicated. ( B ) Normalized PIFE values obtained with different probes incubated with BSA or BamHI. Error bars indicate standard deviations from measurements of three independent wells. P value was calculated by two-tailed t-test. ( C ) Effect of divalent cations on BamHI binding and cleavage. A 25 bp oligonucleotide with the restriction site 1 bp from Cy3 was used as a probe. Chart shows normalized PIFE values obtained upon addition of BamHI and after the subsequent wash. Error bars indicate standard deviations from measurements of three independent wells.

    Journal: Scientific Reports

    Article Title: A rapid method for detecting protein-nucleic acid interactions by protein induced fluorescence enhancement

    doi: 10.1038/srep39653

    Figure Lengend Snippet: Detection of BamHI/DNA interaction by mwPIFE. ( A ) Scheme of DNA probes for detecting BamHI binding. Position of the BamHI restriction site is indicated. ( B ) Normalized PIFE values obtained with different probes incubated with BSA or BamHI. Error bars indicate standard deviations from measurements of three independent wells. P value was calculated by two-tailed t-test. ( C ) Effect of divalent cations on BamHI binding and cleavage. A 25 bp oligonucleotide with the restriction site 1 bp from Cy3 was used as a probe. Chart shows normalized PIFE values obtained upon addition of BamHI and after the subsequent wash. Error bars indicate standard deviations from measurements of three independent wells.

    Article Snippet: After the scan, the buffer was replaced with 100 ul of a buffer containing appropriate protein BamHI (50 U), BSA (15 pmol, Biorad), Ku (0.5–20 pmol) or XPF/ERCC1 (25 pmol).

    Techniques: Binding Assay, Incubation, Two Tailed Test

    Radioactivity registered after chromatography of serum samples containing the RAM–nanodiamond–BSA I125 complex: 1 —sorbent with immobilized BSA, 2 —sorbent with immobilized mouse IgG (percentage of radioactivity of the initial samples used for chromatography)

    Journal: Nanoscale Research Letters

    Article Title: Nanodiamonds as Carriers for Address Delivery of Biologically Active Substances

    doi: 10.1007/s11671-010-9526-0

    Figure Lengend Snippet: Radioactivity registered after chromatography of serum samples containing the RAM–nanodiamond–BSA I125 complex: 1 —sorbent with immobilized BSA, 2 —sorbent with immobilized mouse IgG (percentage of radioactivity of the initial samples used for chromatography)

    Article Snippet: Mouse immunoglobulin G (IgG) (Medix Biochemical, Finland); bovine blood serum (Mikrogen, Russia); BSA (Amersham , England); Sepharose 6B (Pharmacia, Sweden); Rabbit AntiMouse Antibody (RAM) (Imtek Ltd., Russia); NaI125 (RITVERC, Russia); chloramine-T and sodium metabisulphite (Amersham , England) were used as acquired.

    Techniques: Radioactivity, Chromatography

    The ARC CARD is required for the ability of ARC to suppress TNF α -induced necrosis. ( a ) Immunoblot showing inhibition of TNF α -induced HMGB1 release by wild-type ARC but not by the CARD-defective double-point ARC mutant (L31F; G69R). L929 cells were stably transduced with empty vector (Φ), ARC-HA (ARC) or ARC-HA double-point CARD mutant (DM). Immunblot of bovine serum albumin (BSA) in media used as loading control. ( b ) Inhibition of TNF α -induced LDH release by ARC requires the CARD. Data shown as mean±S.E. from n =4. *** P -value

    Journal: Cell Death and Differentiation

    Article Title: A novel role for the apoptosis inhibitor ARC in suppressing TNFα-induced regulated necrosis

    doi: 10.1038/cdd.2013.195

    Figure Lengend Snippet: The ARC CARD is required for the ability of ARC to suppress TNF α -induced necrosis. ( a ) Immunoblot showing inhibition of TNF α -induced HMGB1 release by wild-type ARC but not by the CARD-defective double-point ARC mutant (L31F; G69R). L929 cells were stably transduced with empty vector (Φ), ARC-HA (ARC) or ARC-HA double-point CARD mutant (DM). Immunblot of bovine serum albumin (BSA) in media used as loading control. ( b ) Inhibition of TNF α -induced LDH release by ARC requires the CARD. Data shown as mean±S.E. from n =4. *** P -value

    Article Snippet: Primary antibodies include: ARC (Cayman Chemical, Ann Arbor, MI, USA); β -actin (Sigma-Aldrich, St. Louis, MO, USA); HA (Roche Applied Science, Indianapolis, IN, USA); BSA, HMGB1, His (Abcam, Cambridge, MA, USA); histone H3, PARP, p65 (Cell Signaling Technology, Danvers, MA, USA); RhoGDI α (A-20) (Santa Cruz Biotechnology, Dallas, TX, USA); mouse RIP3 (Prosci, Poway, CA, USA); human RIP3 (Thermo Scientific); RIP1 (BD Biosciences, San Jose, CA, USA); and FADD (Enzo Life Sciences, Farmingdale, NY, USA).

    Techniques: Inhibition, Mutagenesis, Stable Transfection, Transduction, Plasmid Preparation

    TAG-1 and Contactin bind to CD24 and are receptors for Lewis x . A , Lysates from CHO cells transfected with TAX-1 (CHO/TAX-1), Contactin (CHO/Contactin), or NCAM120 (CHO/NCAM120) were incubated with epoxy beads coated with Lewis x –BSA (Le x -BSA), N -acetyllactosamine–BSA (LN-BSA), and BSA. After pull-down, beads were analyzed by Western blotting using antibodies to TAG-1 (lanes 1–4), Contactin (lanes 5–8), or NCAM (lanes 9–12). B , Immunoprecipitates from brain homogenate using CD24 antibody (α-CD24) or control rat IgG (rIgG) were analyzed by Western blotting using antibodies against L1, TAG-1, Contactin, NCAM, or CD24. C , CD24-coupled epoxy beads were incubated with TAX-1-Fc, Contactin-Fc, CHL1-Fc, or Fc alone. After pull-down, beads (PD CD24) were analyzed by Western blotting using HRP-coupled anti-human antibody. D , E , Cerebellar tissue (input) was used for immunoprecipitations with CD24 with antibody (α-CD24), with control rat IgG (rIgG) or without antibody (−) or for immunoprecipitations with TAG-1 antibody (α-TAG-1), Contactin antibody (α-Contactin), or control goat IgG (gIgG) or without antibody (−). Immunoprecipitates were analyzed by Western blotting using antibodies against CD24, TAG-1, Contactin, or CHL1.

    Journal: The Journal of Neuroscience

    Article Title: Lewisx and α2,3-Sialyl Glycans and Their Receptors TAG-1, Contactin, and L1 Mediate CD24-Dependent Neurite Outgrowth

    doi: 10.1523/JNEUROSCI.4361-08.2009

    Figure Lengend Snippet: TAG-1 and Contactin bind to CD24 and are receptors for Lewis x . A , Lysates from CHO cells transfected with TAX-1 (CHO/TAX-1), Contactin (CHO/Contactin), or NCAM120 (CHO/NCAM120) were incubated with epoxy beads coated with Lewis x –BSA (Le x -BSA), N -acetyllactosamine–BSA (LN-BSA), and BSA. After pull-down, beads were analyzed by Western blotting using antibodies to TAG-1 (lanes 1–4), Contactin (lanes 5–8), or NCAM (lanes 9–12). B , Immunoprecipitates from brain homogenate using CD24 antibody (α-CD24) or control rat IgG (rIgG) were analyzed by Western blotting using antibodies against L1, TAG-1, Contactin, NCAM, or CD24. C , CD24-coupled epoxy beads were incubated with TAX-1-Fc, Contactin-Fc, CHL1-Fc, or Fc alone. After pull-down, beads (PD CD24) were analyzed by Western blotting using HRP-coupled anti-human antibody. D , E , Cerebellar tissue (input) was used for immunoprecipitations with CD24 with antibody (α-CD24), with control rat IgG (rIgG) or without antibody (−) or for immunoprecipitations with TAG-1 antibody (α-TAG-1), Contactin antibody (α-Contactin), or control goat IgG (gIgG) or without antibody (−). Immunoprecipitates were analyzed by Western blotting using antibodies against CD24, TAG-1, Contactin, or CHL1.

    Article Snippet: For pull-down assays, 80 μg of Lewisx –BSA, N -acetyllactosamine–BSA, or BSA (Sigma) was coated onto Dynabeads M-270 Epoxy (Dynal) by overnight incubation at 4°C.

    Techniques: Transfection, Incubation, Western Blot

    Effect of BSA type on the TaqMan-based PCR yield. Acetylated BSA from Ambion and Promega, non-acetylated BSA from Sigma were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).

    Journal: Biomicrofluidics

    Article Title: Acetylated bovine serum albumin differentially inhibits polymerase chain reaction in microdevices

    doi: 10.1063/1.4983692

    Figure Lengend Snippet: Effect of BSA type on the TaqMan-based PCR yield. Acetylated BSA from Ambion and Promega, non-acetylated BSA from Sigma were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).

    Article Snippet: The PCR solution (20 μ l) contained 1× LuminoCt qPCR ReadyMix (Sigma Aldrich, Singapore), 1× ROX internal reference dye (Sigma Aldrich, Singapore), TaqMan probe for the nuc gene at 250 nM, forward and reverse primers at 900 nM each, 1 μ g/ μ l of either acetylated BSA (from two vendors: Ambion AM2614 and Promega P/N R3961) or non-acetylated BSA (Sigma-Aldrich P/N B8667), and 1 ng/ μ l of genomic DNA isolated from Staphylococcus aureus subsp. aureus ATCC 33591 (MRSA, strain 328; ATCC).

    Techniques: Polymerase Chain Reaction, Concentration Assay