bs-7861R Search Results


eme1  (Bioss)
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Bioss eme1
Upregulation of <t>EME1</t> reflects reduced survival in clinical GC. (a) Volcano plot was drawn to show differentially expressed genes, including 1110 upregulated and 1566 downregulated GC genes. (b) Top 10 upregulated and downregulated mRNAs between GC and adjacent tumor tissue samples in the heat map. (c) Quantitation of EME1 amounts in 407 pairs of noncancerous and GC tissue specimens derived from the TCGA database. The y-axis reflects EME1 staining intensity. (d) Kaplan–Meier curves for low and high EME1 expression groups in GC cases. (e) Representative micrographs of the EME1 expression in adjacent non-cancerous and primary GC tissue samples assessed via IHC staining. Nuclei appear blue and positive expression sites are brownish-yellow. Scale bars are 50 μm. (f) Semi-quantitative analysis of the EME1 expression in adjacent noncancerous and primary GC tissue samples. ns: p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Data are χ ± SD from three measurements. All data points were measured in triplicate
Eme1, supplied by Bioss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
eme1 - by Bioz Stars, 2026-02
86/100 stars
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eme1 polyclonal antibody  (Bioss)
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EME1 complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein (MUS81) to form an endonuclease complex which cleaves branched DNA structures, especially those arising during stalled DNA replication. The protein may be involved in repairing DNA damage
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Upregulation of EME1 reflects reduced survival in clinical GC. (a) Volcano plot was drawn to show differentially expressed genes, including 1110 upregulated and 1566 downregulated GC genes. (b) Top 10 upregulated and downregulated mRNAs between GC and adjacent tumor tissue samples in the heat map. (c) Quantitation of EME1 amounts in 407 pairs of noncancerous and GC tissue specimens derived from the TCGA database. The y-axis reflects EME1 staining intensity. (d) Kaplan–Meier curves for low and high EME1 expression groups in GC cases. (e) Representative micrographs of the EME1 expression in adjacent non-cancerous and primary GC tissue samples assessed via IHC staining. Nuclei appear blue and positive expression sites are brownish-yellow. Scale bars are 50 μm. (f) Semi-quantitative analysis of the EME1 expression in adjacent noncancerous and primary GC tissue samples. ns: p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Data are χ ± SD from three measurements. All data points were measured in triplicate

Journal: Bioengineered

Article Title: Essential meiotic structure-specific endonuclease1 ( EME1 ) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway

doi: 10.1080/21655979.2021.1999371

Figure Lengend Snippet: Upregulation of EME1 reflects reduced survival in clinical GC. (a) Volcano plot was drawn to show differentially expressed genes, including 1110 upregulated and 1566 downregulated GC genes. (b) Top 10 upregulated and downregulated mRNAs between GC and adjacent tumor tissue samples in the heat map. (c) Quantitation of EME1 amounts in 407 pairs of noncancerous and GC tissue specimens derived from the TCGA database. The y-axis reflects EME1 staining intensity. (d) Kaplan–Meier curves for low and high EME1 expression groups in GC cases. (e) Representative micrographs of the EME1 expression in adjacent non-cancerous and primary GC tissue samples assessed via IHC staining. Nuclei appear blue and positive expression sites are brownish-yellow. Scale bars are 50 μm. (f) Semi-quantitative analysis of the EME1 expression in adjacent noncancerous and primary GC tissue samples. ns: p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Data are χ ± SD from three measurements. All data points were measured in triplicate

Article Snippet: GC tissues were fixed with 10% formalin and incubated with specific primary antibodies, including EME1 (Bioss Bs-7861 R) and Ki-67 (Servicebio GB13030-2), following paraffin embedding.

Techniques: Quantitation Assay, Derivative Assay, Staining, Expressing, Immunohistochemistry

Associations of EME1 expression in GC tissue samples with clinicopathological parameters

Journal: Bioengineered

Article Title: Essential meiotic structure-specific endonuclease1 ( EME1 ) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway

doi: 10.1080/21655979.2021.1999371

Figure Lengend Snippet: Associations of EME1 expression in GC tissue samples with clinicopathological parameters

Article Snippet: GC tissues were fixed with 10% formalin and incubated with specific primary antibodies, including EME1 (Bioss Bs-7861 R) and Ki-67 (Servicebio GB13030-2), following paraffin embedding.

Techniques: Expressing

EME1 silencing suppresses proliferation and enhances apoptosis in GC cells. (a, b) Cell proliferation as assessed by colony formation and CCK-8 assays, respectively. (c) Cell cycle distribution examined via flow cytometry. Cell rates in the G0/G1, S, and G2/M phases are shown. (d, e) AnnexinⅤ-FITC/PI double-staining and flow cytometry, respectively, showing increased apoptosis in EME1 -silenced cells compared with null cells in both lines. (f) Immunofluorescence used to detect EME1 ; nuclei were stained with DAPI. Scale bars represent 10 µm. ns: p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. Data are presented as the mean ±SD from three measurements

Journal: Bioengineered

Article Title: Essential meiotic structure-specific endonuclease1 ( EME1 ) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway

doi: 10.1080/21655979.2021.1999371

Figure Lengend Snippet: EME1 silencing suppresses proliferation and enhances apoptosis in GC cells. (a, b) Cell proliferation as assessed by colony formation and CCK-8 assays, respectively. (c) Cell cycle distribution examined via flow cytometry. Cell rates in the G0/G1, S, and G2/M phases are shown. (d, e) AnnexinⅤ-FITC/PI double-staining and flow cytometry, respectively, showing increased apoptosis in EME1 -silenced cells compared with null cells in both lines. (f) Immunofluorescence used to detect EME1 ; nuclei were stained with DAPI. Scale bars represent 10 µm. ns: p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. Data are presented as the mean ±SD from three measurements

Article Snippet: GC tissues were fixed with 10% formalin and incubated with specific primary antibodies, including EME1 (Bioss Bs-7861 R) and Ki-67 (Servicebio GB13030-2), following paraffin embedding.

Techniques: CCK-8 Assay, Flow Cytometry, Double Staining, Immunofluorescence, Staining

EME1 promotes migration and invasion in cultured GC cells. (a, b) Scratch assay showing reduced migration rates in the AGS and MGC-803 cell lines, respectively, compared with the NC group 24 h after downregulation of EME1 . (c, e) Transwell assay demonstrating that EME1 silencing remarkably decreased the migration rates of the AGS and MGC-803 cell lines, respectively, compared with the control. (d, e) Transwell assay showing decreased cell invasion and migration, respectively following the downregulation of EME1 . ns: p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. Data are presented as the mean ±SD from three measurements

Journal: Bioengineered

Article Title: Essential meiotic structure-specific endonuclease1 ( EME1 ) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway

doi: 10.1080/21655979.2021.1999371

Figure Lengend Snippet: EME1 promotes migration and invasion in cultured GC cells. (a, b) Scratch assay showing reduced migration rates in the AGS and MGC-803 cell lines, respectively, compared with the NC group 24 h after downregulation of EME1 . (c, e) Transwell assay demonstrating that EME1 silencing remarkably decreased the migration rates of the AGS and MGC-803 cell lines, respectively, compared with the control. (d, e) Transwell assay showing decreased cell invasion and migration, respectively following the downregulation of EME1 . ns: p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. Data are presented as the mean ±SD from three measurements

Article Snippet: GC tissues were fixed with 10% formalin and incubated with specific primary antibodies, including EME1 (Bioss Bs-7861 R) and Ki-67 (Servicebio GB13030-2), following paraffin embedding.

Techniques: Migration, Cell Culture, Wound Healing Assay, Transwell Assay

EME1 silencing inhibits tumor cell proliferation in vivo. (a) AGS xenograft sizes at 30 d after treatment with Si- EME1 and NC transfected cells. (b) Tumor growth curves of AGS xenografts in the Si- EME1 and NC groups. (c) Tumor weights in the Si- EME1 and NC groups. (d) Representative bioluminescence images in both groups eight weeks following the tail vein injections of cells. (e) Immunohistochemical staining used to detect Ki-67 levels in transplanted xerographs of the Si-EME1 and NC groups (original magnification, ×200). (f) Mean optical density values of Ki-67 in both groups. Data are χ ± SD. t-test: *P < 0.05, **P < 0.01, ***P < 0.001. All data points were measured in triplicate

Journal: Bioengineered

Article Title: Essential meiotic structure-specific endonuclease1 ( EME1 ) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway

doi: 10.1080/21655979.2021.1999371

Figure Lengend Snippet: EME1 silencing inhibits tumor cell proliferation in vivo. (a) AGS xenograft sizes at 30 d after treatment with Si- EME1 and NC transfected cells. (b) Tumor growth curves of AGS xenografts in the Si- EME1 and NC groups. (c) Tumor weights in the Si- EME1 and NC groups. (d) Representative bioluminescence images in both groups eight weeks following the tail vein injections of cells. (e) Immunohistochemical staining used to detect Ki-67 levels in transplanted xerographs of the Si-EME1 and NC groups (original magnification, ×200). (f) Mean optical density values of Ki-67 in both groups. Data are χ ± SD. t-test: *P < 0.05, **P < 0.01, ***P < 0.001. All data points were measured in triplicate

Article Snippet: GC tissues were fixed with 10% formalin and incubated with specific primary antibodies, including EME1 (Bioss Bs-7861 R) and Ki-67 (Servicebio GB13030-2), following paraffin embedding.

Techniques: In Vivo, Transfection, Immunohistochemical staining, Staining

EME1 regulates Akt-GSK3B-CCND1 pathway. (a) KEGG enrichment analysis of 20 important pathways regulated by EME1 in GC cells. (b) Heat map of EME1 co-expression in GC tissues. (c) EME1 and Akt1 as positively correlated in GC tissues (P < 0.001). (d) qRT-PCR and immunoblot analyses of EME1 and Akt1. (e) Immunoblot of Akt in the rescue experiment. (f) EME1 knockdown downregulating major Akt pathway effectors. *P < 0.05, **P < 0.01

Journal: Bioengineered

Article Title: Essential meiotic structure-specific endonuclease1 ( EME1 ) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway

doi: 10.1080/21655979.2021.1999371

Figure Lengend Snippet: EME1 regulates Akt-GSK3B-CCND1 pathway. (a) KEGG enrichment analysis of 20 important pathways regulated by EME1 in GC cells. (b) Heat map of EME1 co-expression in GC tissues. (c) EME1 and Akt1 as positively correlated in GC tissues (P < 0.001). (d) qRT-PCR and immunoblot analyses of EME1 and Akt1. (e) Immunoblot of Akt in the rescue experiment. (f) EME1 knockdown downregulating major Akt pathway effectors. *P < 0.05, **P < 0.01

Article Snippet: GC tissues were fixed with 10% formalin and incubated with specific primary antibodies, including EME1 (Bioss Bs-7861 R) and Ki-67 (Servicebio GB13030-2), following paraffin embedding.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Silencing of EME1 inhibits GC progression in vivo through downregulation of Akt. (a) Overexpression of Akt promoting the proliferation of AGS and MGC-803 cells, in contrast to the EME1 silencing group. (b, c) G0/G1 and S and G2/M phase rates, respectively, following Akt overexpression; these rates show opposite trends to the results of the EME1 silencing group, as shown via flow-cytometry. (d, e) Flow cytometry demonstrating that the overexpression of Akt affected apoptosis in AGS and MGC cells, respectively, with a trend opposite to that of the EME1 silencing group. (f, g) Colony formation assay. The overexpression of Akt altered the number of visible colonies in AGS and MGC cells, respectively with a trend opposite to that of the EME1 silencing group. All assays were repeated thrice. Data are presented as the mean ±SD. *p < 0.05

Journal: Bioengineered

Article Title: Essential meiotic structure-specific endonuclease1 ( EME1 ) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway

doi: 10.1080/21655979.2021.1999371

Figure Lengend Snippet: Silencing of EME1 inhibits GC progression in vivo through downregulation of Akt. (a) Overexpression of Akt promoting the proliferation of AGS and MGC-803 cells, in contrast to the EME1 silencing group. (b, c) G0/G1 and S and G2/M phase rates, respectively, following Akt overexpression; these rates show opposite trends to the results of the EME1 silencing group, as shown via flow-cytometry. (d, e) Flow cytometry demonstrating that the overexpression of Akt affected apoptosis in AGS and MGC cells, respectively, with a trend opposite to that of the EME1 silencing group. (f, g) Colony formation assay. The overexpression of Akt altered the number of visible colonies in AGS and MGC cells, respectively with a trend opposite to that of the EME1 silencing group. All assays were repeated thrice. Data are presented as the mean ±SD. *p < 0.05

Article Snippet: GC tissues were fixed with 10% formalin and incubated with specific primary antibodies, including EME1 (Bioss Bs-7861 R) and Ki-67 (Servicebio GB13030-2), following paraffin embedding.

Techniques: In Vivo, Over Expression, Flow Cytometry, Colony Assay

MYB interacts with the EME1 promoter and negatively regulates EME1 in GC cells. (a) Predicted binding sites of EME1 to MYB. (b) Quantification of MYB levels in 407 pairs of noncancerous and GC tissue specimens derived from the TCGA database. The y-axis shows the MYB staining intensity. (c) MYB mRNA expression levels in GC cells (MGC-803 and AGS), as detected by qRT-PCR. (d, e) Results of overexpression (d) or knockdown MYB (e) showing that MYB negatively regulated EME1 expression. (f) EME1 and MYB as examined by Western blotting in AGS cells transfected with si-Ctrl and si-MYB. (g) MYB transfected into 293 T cells, and the promoter activity of EME1 was assessed using a dual-luciferase reporter gene assay. *P < 0.05, **P < 0.01, ***P < 0.001

Journal: Bioengineered

Article Title: Essential meiotic structure-specific endonuclease1 ( EME1 ) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway

doi: 10.1080/21655979.2021.1999371

Figure Lengend Snippet: MYB interacts with the EME1 promoter and negatively regulates EME1 in GC cells. (a) Predicted binding sites of EME1 to MYB. (b) Quantification of MYB levels in 407 pairs of noncancerous and GC tissue specimens derived from the TCGA database. The y-axis shows the MYB staining intensity. (c) MYB mRNA expression levels in GC cells (MGC-803 and AGS), as detected by qRT-PCR. (d, e) Results of overexpression (d) or knockdown MYB (e) showing that MYB negatively regulated EME1 expression. (f) EME1 and MYB as examined by Western blotting in AGS cells transfected with si-Ctrl and si-MYB. (g) MYB transfected into 293 T cells, and the promoter activity of EME1 was assessed using a dual-luciferase reporter gene assay. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: GC tissues were fixed with 10% formalin and incubated with specific primary antibodies, including EME1 (Bioss Bs-7861 R) and Ki-67 (Servicebio GB13030-2), following paraffin embedding.

Techniques: Binding Assay, Derivative Assay, Staining, Expressing, Quantitative RT-PCR, Over Expression, Western Blot, Transfection, Activity Assay, Luciferase, Reporter Gene Assay