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vav2  (Bioss)
90
Bioss vav2
Figure 3. Rac1 activation by Hh via <t>Vav2.</t> (A) C3H10T1/2 cells were transiently transfected with the indicated plasmids. Total cell lysates (Input) and anti-Myc immunoprecipitates (IP) from total cell lysates were analyzed by immunoblotting with anti-Myc and anti-Smo antibodies. (B) C3H10T1/2 cells were cultured with or without N-Shh at 100 ng/ml for 24 h. Total cell lysates (Input) and anti-Vav2 immunoprecipitates (IP, Vav2 Ab, +) from total cell lysates were analyzed by immunoblotting with anti-Vav2
Vav2, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 3. Rac1 activation by Hh via Vav2. (A) C3H10T1/2 cells were transiently transfected with the indicated plasmids. Total cell lysates (Input) and anti-Myc immunoprecipitates (IP) from total cell lysates were analyzed by immunoblotting with anti-Myc and anti-Smo antibodies. (B) C3H10T1/2 cells were cultured with or without N-Shh at 100 ng/ml for 24 h. Total cell lysates (Input) and anti-Vav2 immunoprecipitates (IP, Vav2 Ab, +) from total cell lysates were analyzed by immunoblotting with anti-Vav2

Journal: Theranostics

Article Title: Hedgehog signaling is controlled by Rac1 activity.

doi: 10.7150/thno.67702

Figure Lengend Snippet: Figure 3. Rac1 activation by Hh via Vav2. (A) C3H10T1/2 cells were transiently transfected with the indicated plasmids. Total cell lysates (Input) and anti-Myc immunoprecipitates (IP) from total cell lysates were analyzed by immunoblotting with anti-Myc and anti-Smo antibodies. (B) C3H10T1/2 cells were cultured with or without N-Shh at 100 ng/ml for 24 h. Total cell lysates (Input) and anti-Vav2 immunoprecipitates (IP, Vav2 Ab, +) from total cell lysates were analyzed by immunoblotting with anti-Vav2

Article Snippet: Antibodies for PAK1, phospho-PAK1 and phospho-Ser/Thr were from Cell Signaling Technology; IFT88, KIF3A, SuFu, c-Myc, Gli1, acetylated-αTubulin (Ac-Tub), Vav2, GAPDH and β-actin antibdies were from Santa Cruz Biotechnology; Ptch1 antibody, Smo antibody and phospho-Vav2 antibody were from Abcam; Gli2 antibody and Rac1 antibody were from Bioss (Beijing, China); Rac2 and Rac3 antibodies were purchased from Huabio (Hangzhou, China); Flag-tag and HA-tag antibodies were from Beyotime (Shanghai, China); GST-tag antibody was from Yeason (Shanghai, China), and antibody anti-Arl13b was from Proteintech.

Techniques: Activation Assay, Transfection, Western Blot, Cell Culture