bs-2514R Search Results


ccr5  (Bioss)
94
Bioss ccr5
Primers used for real-time polymerase chain reaction.
Ccr5, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Primers used for real-time polymerase chain reaction.

Journal: PLoS ONE

Article Title: The enhancement of CCL2 and CCL5 by human bone marrow-derived mesenchymal stem/stromal cells might contribute to inflammatory suppression and axonal extension after spinal cord injury

doi: 10.1371/journal.pone.0230080

Figure Lengend Snippet: Primers used for real-time polymerase chain reaction.

Article Snippet: CCR5 , Rabbit , Bioss (Boston, MA) , bs-2514R , 500.

Techniques:

Primary antibodies used for immunohistochemistry.

Journal: PLoS ONE

Article Title: The enhancement of CCL2 and CCL5 by human bone marrow-derived mesenchymal stem/stromal cells might contribute to inflammatory suppression and axonal extension after spinal cord injury

doi: 10.1371/journal.pone.0230080

Figure Lengend Snippet: Primary antibodies used for immunohistochemistry.

Article Snippet: CCR5 , Rabbit , Bioss (Boston, MA) , bs-2514R , 500.

Techniques: Immunohistochemistry

Expression of Ccl2 (A) , Ccl3 (B) , Ccl4 (C) , Ccl7 (E) , Cxcl1 (F) , and Cxcl2 (G) significantly increased on post-operative day (pod) 1. In contrast, increases in Ccl5 (D) expression were observed on pod 14. Increases in gene expression were observed for all chemokine receptors (H—L) on pod 1. While Ccr1 (H) , Ccr3 (J) , and Cxcr2 (L) expression decreased by pod 7, Ccr2 (I) and Ccr5 (K) levels remained significantly elevated on pod 14. All data are expressed the relative level (fold) to compare with pod 0 (basal level) after normalization against Gapdh as a housekeeping gene (n = 6 mouse at each time-point). Data are expressed as mean ± SEM. *: P<0 . 05 , **: P<0 . 01 (Dunnett’s post hoc test, vs day 0).

Journal: PLoS ONE

Article Title: The enhancement of CCL2 and CCL5 by human bone marrow-derived mesenchymal stem/stromal cells might contribute to inflammatory suppression and axonal extension after spinal cord injury

doi: 10.1371/journal.pone.0230080

Figure Lengend Snippet: Expression of Ccl2 (A) , Ccl3 (B) , Ccl4 (C) , Ccl7 (E) , Cxcl1 (F) , and Cxcl2 (G) significantly increased on post-operative day (pod) 1. In contrast, increases in Ccl5 (D) expression were observed on pod 14. Increases in gene expression were observed for all chemokine receptors (H—L) on pod 1. While Ccr1 (H) , Ccr3 (J) , and Cxcr2 (L) expression decreased by pod 7, Ccr2 (I) and Ccr5 (K) levels remained significantly elevated on pod 14. All data are expressed the relative level (fold) to compare with pod 0 (basal level) after normalization against Gapdh as a housekeeping gene (n = 6 mouse at each time-point). Data are expressed as mean ± SEM. *: P<0 . 05 , **: P<0 . 01 (Dunnett’s post hoc test, vs day 0).

Article Snippet: CCR5 , Rabbit , Bioss (Boston, MA) , bs-2514R , 500.

Techniques: Expressing

(A) CCL5 + cells were observed at the peri-injury site on pod 14 and were merged with NeuN + ( red ) and F4/80 + ( red ) cells, respectively (arrows). However, CCL5 + cells were not merged with GAFP + cells ( red ). (B) CCR5 + immunoreactivity ( green ) was observed in the epicenter on pod 1, and merged with Ly-6G + cells ( red ) (arrows). (C) CCR5 + cells ( green ) were observed at the peri-injury site on pod 14, and were merged with NeuN + ( red with arrows) and F4/80 + (arrows with dashed lines) cells. (D) F4/80 + cells ( red ) on pod 14 co-stained with Ym1 + immunoreactions ( green ), a marker for M2-type alternatively activated MG/MΦ (arrows). Nuclei were detected using DAPI.

Journal: PLoS ONE

Article Title: The enhancement of CCL2 and CCL5 by human bone marrow-derived mesenchymal stem/stromal cells might contribute to inflammatory suppression and axonal extension after spinal cord injury

doi: 10.1371/journal.pone.0230080

Figure Lengend Snippet: (A) CCL5 + cells were observed at the peri-injury site on pod 14 and were merged with NeuN + ( red ) and F4/80 + ( red ) cells, respectively (arrows). However, CCL5 + cells were not merged with GAFP + cells ( red ). (B) CCR5 + immunoreactivity ( green ) was observed in the epicenter on pod 1, and merged with Ly-6G + cells ( red ) (arrows). (C) CCR5 + cells ( green ) were observed at the peri-injury site on pod 14, and were merged with NeuN + ( red with arrows) and F4/80 + (arrows with dashed lines) cells. (D) F4/80 + cells ( red ) on pod 14 co-stained with Ym1 + immunoreactions ( green ), a marker for M2-type alternatively activated MG/MΦ (arrows). Nuclei were detected using DAPI.

Article Snippet: CCR5 , Rabbit , Bioss (Boston, MA) , bs-2514R , 500.

Techniques: Staining, Marker

Expression of mouse (A) Ccl5 and (B) Ccr5 in the spinal cord was quantified via real-time PCR in a time-dependent fashion after spinal cord injury (SCI) and hMSC injection. Mice treated with hMSCs (n = 19 mouse) exhibited significant increases in Ccl5 expression relative to HBSS-treated mice (n = 13 mouse) on postoperative day (pod) 14. However, no significant differences in Ccr5 expression were observed between the two groups during the experimental period. Data are expressed the relative level (fold) to compare with pod 0 (basal level, n = 6 mouse) after normalization against Gapdh as a housekeeping gene. Data are expressed as the mean ± SEM. **: P < 0 . 01 (Dunnett’s post hoc test), n.s.: not significant. (C) CCL5 immunoreactivity ( green ) was observed at the peri-injury site on pod 14 and merged with NeuN + ( red ) and F4/80 + ( pink ) cells. Mice treated with hMSCs exhibited greater CCL5 + immunoreactivity than did HBSS-treated mice. (D) Similar CCR5 + reactions ( green ) were merged with F4/80 + ( red ) cells in both experimental groups. Blue indicates nuclear staining using DAPI.

Journal: PLoS ONE

Article Title: The enhancement of CCL2 and CCL5 by human bone marrow-derived mesenchymal stem/stromal cells might contribute to inflammatory suppression and axonal extension after spinal cord injury

doi: 10.1371/journal.pone.0230080

Figure Lengend Snippet: Expression of mouse (A) Ccl5 and (B) Ccr5 in the spinal cord was quantified via real-time PCR in a time-dependent fashion after spinal cord injury (SCI) and hMSC injection. Mice treated with hMSCs (n = 19 mouse) exhibited significant increases in Ccl5 expression relative to HBSS-treated mice (n = 13 mouse) on postoperative day (pod) 14. However, no significant differences in Ccr5 expression were observed between the two groups during the experimental period. Data are expressed the relative level (fold) to compare with pod 0 (basal level, n = 6 mouse) after normalization against Gapdh as a housekeeping gene. Data are expressed as the mean ± SEM. **: P < 0 . 01 (Dunnett’s post hoc test), n.s.: not significant. (C) CCL5 immunoreactivity ( green ) was observed at the peri-injury site on pod 14 and merged with NeuN + ( red ) and F4/80 + ( pink ) cells. Mice treated with hMSCs exhibited greater CCL5 + immunoreactivity than did HBSS-treated mice. (D) Similar CCR5 + reactions ( green ) were merged with F4/80 + ( red ) cells in both experimental groups. Blue indicates nuclear staining using DAPI.

Article Snippet: CCR5 , Rabbit , Bioss (Boston, MA) , bs-2514R , 500.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Injection, Staining