bs-13209R-Biotin Search Results


ccl5  (Bioss)
90
Bioss ccl5
Lack of CD8α + DCs led to decreased IFN-γ and <t>CCL5</t> expression in the aorta. The Apoe −/− mice (n = 8) and the Batf3 −/− Apoe −/− mice (n = 8) were fed either a chow diet or Western diet for 12 weeks. (a) Analysis of aortic Ifng , Foxp3 , and Tbx21 mRNA expression by qPCR. (b) An aortic tissue ELISA analysis of IFN-γ. Data represent pg/mg aortic protein. (c) qPCR analysis of Il12a , Tnf , Il6 , Tgfb1 , and Il10 mRNA expression. (d) qPCR analysis of Ccl5 expression. (e) ELISA analysis of the CCL5 concentration in the aortic tissue. Data represent pg per mg aortic protein. Data are presented as mean ± SD. Differences of a P < 0.05 were considered to be statistically significant. * P < 0.05; ** P < 0.01; *** P < 0.001; ns, not significant. Data are representative of three independent experiments. See also Fig. S6.
Ccl5, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccl5/product/Bioss
Average 90 stars, based on 1 article reviews
ccl5 - by Bioz Stars, 2026-02
90/100 stars
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anti zo 1  (Bioss)
86
Bioss anti zo 1
0.025%, 0.05% and 0.1% DHM maintained the barrier function in ileum. Original blots for <t>ZO-1,</t> occluding, claudin-1 and GAPDH ( A ). Changes of ZO-1 ( B ), occludin ( C ) and claudin-1( D ) protein expression levels in the ileum after 60 mg/kg LPS exposure for 12 h followed by 14 days of 0.025%, 0.05% and 0.1% DHM treatment. Values are expressed as the mean ± SD for each group ( n = 5). * p < 0.05 and ** p < 0.01 represented all groups compared with the control group. # p < 0.05 and ## p < 0.01 represented all groups compared with the LPS group.
Anti Zo 1, supplied by Bioss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti zo 1/product/Bioss
Average 86 stars, based on 1 article reviews
anti zo 1 - by Bioz Stars, 2026-02
86/100 stars
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g6pc3 polyclonal antibody, biotin conjugated  (Bioss)
90
Bioss g6pc3 polyclonal antibody, biotin conjugated
0.025%, 0.05% and 0.1% DHM maintained the barrier function in ileum. Original blots for <t>ZO-1,</t> occluding, claudin-1 and GAPDH ( A ). Changes of ZO-1 ( B ), occludin ( C ) and claudin-1( D ) protein expression levels in the ileum after 60 mg/kg LPS exposure for 12 h followed by 14 days of 0.025%, 0.05% and 0.1% DHM treatment. Values are expressed as the mean ± SD for each group ( n = 5). * p < 0.05 and ** p < 0.01 represented all groups compared with the control group. # p < 0.05 and ## p < 0.01 represented all groups compared with the LPS group.
G6pc3 Polyclonal Antibody, Biotin Conjugated, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/g6pc3 polyclonal antibody, biotin conjugated/product/Bioss
Average 90 stars, based on 1 article reviews
g6pc3 polyclonal antibody, biotin conjugated - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
foxred1 polyclonal antibody, biotin conjugated  (Bioss)
N/A
FOXRED1 is a 486 amino acid single-pass membrane protein. Utilizing FAD as a cofactor, FOXRED1 may act as a chaperone protein essential for the function of mitochondrial complex I. Mutations to FOXRED1 may result in
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Image Search Results


Lack of CD8α + DCs led to decreased IFN-γ and CCL5 expression in the aorta. The Apoe −/− mice (n = 8) and the Batf3 −/− Apoe −/− mice (n = 8) were fed either a chow diet or Western diet for 12 weeks. (a) Analysis of aortic Ifng , Foxp3 , and Tbx21 mRNA expression by qPCR. (b) An aortic tissue ELISA analysis of IFN-γ. Data represent pg/mg aortic protein. (c) qPCR analysis of Il12a , Tnf , Il6 , Tgfb1 , and Il10 mRNA expression. (d) qPCR analysis of Ccl5 expression. (e) ELISA analysis of the CCL5 concentration in the aortic tissue. Data represent pg per mg aortic protein. Data are presented as mean ± SD. Differences of a P < 0.05 were considered to be statistically significant. * P < 0.05; ** P < 0.01; *** P < 0.001; ns, not significant. Data are representative of three independent experiments. See also Fig. S6.

Journal: EBioMedicine

Article Title: Batf3-dependent CD8α + Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages

doi: 10.1016/j.ebiom.2017.04.008

Figure Lengend Snippet: Lack of CD8α + DCs led to decreased IFN-γ and CCL5 expression in the aorta. The Apoe −/− mice (n = 8) and the Batf3 −/− Apoe −/− mice (n = 8) were fed either a chow diet or Western diet for 12 weeks. (a) Analysis of aortic Ifng , Foxp3 , and Tbx21 mRNA expression by qPCR. (b) An aortic tissue ELISA analysis of IFN-γ. Data represent pg/mg aortic protein. (c) qPCR analysis of Il12a , Tnf , Il6 , Tgfb1 , and Il10 mRNA expression. (d) qPCR analysis of Ccl5 expression. (e) ELISA analysis of the CCL5 concentration in the aortic tissue. Data represent pg per mg aortic protein. Data are presented as mean ± SD. Differences of a P < 0.05 were considered to be statistically significant. * P < 0.05; ** P < 0.01; *** P < 0.001; ns, not significant. Data are representative of three independent experiments. See also Fig. S6.

Article Snippet: For the immunohistofluorescence analysis, the cryosections were stained with an antibody against CD45 (104; eBioscience Cat# 47-0451-82, RRID: AB_1548781 ), Mac3 (M3/84; BD Biosciences Cat# 550292, RRID: AB_393587 ), CCL5 (Bioss Inc. Cat# bs-1324R-Biotin, RRID: AB_11099534 ).

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Concentration Assay

IFN-γ promoted CCL5 expression on macrophages, promoting leukocytes infiltration into the plaque area. (a) Female Apoe −/− mice (n = 15) were put on a Western diet for eight weeks, then the aortic cells were pooled to prepared as described in the methods and stained with an antibody against CD45. Then, CD45 + leukocytes and CD45 − non-leukocytes were sorted by FACS, and qPCR was used to analyze Ccl5 mRNA expression. (b and c) Immunofluorescence of the aortic root. The Apoe −/− mice (n = 8) and the Batf3 −/− Apoe −/− mice (n = 8) were fed a Western diet for 12 weeks. The cryosections of the aortic root were stained with an antibody against CD45 (red), MAC3 (red), and CCL5 (green). The cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Images were viewed and captured with a Laser Scanning Confocal Microscope. Scale bars: 100 μm, dashed lines indicate the internal elastic lamina, arrows pointing to representative colocalized cells. (d) Primary splenic macrophages were sorted as described in supplementary data, and then treated with100 ng/mL IFN-γ for 6 h. Ccl5 mRNA expression was analyzed by qPCR. The Apoe −/− mice (n = 8) and the Batf3 −/− Apoe −/− mice (n = 8) were fed a Western diet for 6 weeks, and cryosections of the aortic root were performed. (e) H&E staining. Scale bars: 100 μm. (f) Immunohistochemistry. Representative images of leukocytes (CD45), T cells (CD3), DCs (CD11c), and macrophages (Mac3) in the aortic are shown. Scale bars: 200 μm. Data are presented as mean ± SD. Differences of a P < 0.05 were considered to be statistically significant. ** P < 0.01; *** P < 0.001; ns, not significant. Data are representative of three independent experiments.

Journal: EBioMedicine

Article Title: Batf3-dependent CD8α + Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages

doi: 10.1016/j.ebiom.2017.04.008

Figure Lengend Snippet: IFN-γ promoted CCL5 expression on macrophages, promoting leukocytes infiltration into the plaque area. (a) Female Apoe −/− mice (n = 15) were put on a Western diet for eight weeks, then the aortic cells were pooled to prepared as described in the methods and stained with an antibody against CD45. Then, CD45 + leukocytes and CD45 − non-leukocytes were sorted by FACS, and qPCR was used to analyze Ccl5 mRNA expression. (b and c) Immunofluorescence of the aortic root. The Apoe −/− mice (n = 8) and the Batf3 −/− Apoe −/− mice (n = 8) were fed a Western diet for 12 weeks. The cryosections of the aortic root were stained with an antibody against CD45 (red), MAC3 (red), and CCL5 (green). The cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Images were viewed and captured with a Laser Scanning Confocal Microscope. Scale bars: 100 μm, dashed lines indicate the internal elastic lamina, arrows pointing to representative colocalized cells. (d) Primary splenic macrophages were sorted as described in supplementary data, and then treated with100 ng/mL IFN-γ for 6 h. Ccl5 mRNA expression was analyzed by qPCR. The Apoe −/− mice (n = 8) and the Batf3 −/− Apoe −/− mice (n = 8) were fed a Western diet for 6 weeks, and cryosections of the aortic root were performed. (e) H&E staining. Scale bars: 100 μm. (f) Immunohistochemistry. Representative images of leukocytes (CD45), T cells (CD3), DCs (CD11c), and macrophages (Mac3) in the aortic are shown. Scale bars: 200 μm. Data are presented as mean ± SD. Differences of a P < 0.05 were considered to be statistically significant. ** P < 0.01; *** P < 0.001; ns, not significant. Data are representative of three independent experiments.

Article Snippet: For the immunohistofluorescence analysis, the cryosections were stained with an antibody against CD45 (104; eBioscience Cat# 47-0451-82, RRID: AB_1548781 ), Mac3 (M3/84; BD Biosciences Cat# 550292, RRID: AB_393587 ), CCL5 (Bioss Inc. Cat# bs-1324R-Biotin, RRID: AB_11099534 ).

Techniques: Expressing, Western Blot, Staining, Immunofluorescence, Microscopy, Immunohistochemistry

0.025%, 0.05% and 0.1% DHM maintained the barrier function in ileum. Original blots for ZO-1, occluding, claudin-1 and GAPDH ( A ). Changes of ZO-1 ( B ), occludin ( C ) and claudin-1( D ) protein expression levels in the ileum after 60 mg/kg LPS exposure for 12 h followed by 14 days of 0.025%, 0.05% and 0.1% DHM treatment. Values are expressed as the mean ± SD for each group ( n = 5). * p < 0.05 and ** p < 0.01 represented all groups compared with the control group. # p < 0.05 and ## p < 0.01 represented all groups compared with the LPS group.

Journal: Veterinary Research

Article Title: Dihydromyricetin attenuates Escherichia coli lipopolysaccharide-induced ileum injury in chickens by inhibiting NLRP3 inflammasome and TLR4/NF-κB signalling pathway

doi: 10.1186/s13567-020-00796-8

Figure Lengend Snippet: 0.025%, 0.05% and 0.1% DHM maintained the barrier function in ileum. Original blots for ZO-1, occluding, claudin-1 and GAPDH ( A ). Changes of ZO-1 ( B ), occludin ( C ) and claudin-1( D ) protein expression levels in the ileum after 60 mg/kg LPS exposure for 12 h followed by 14 days of 0.025%, 0.05% and 0.1% DHM treatment. Values are expressed as the mean ± SD for each group ( n = 5). * p < 0.05 and ** p < 0.01 represented all groups compared with the control group. # p < 0.05 and ## p < 0.01 represented all groups compared with the LPS group.

Article Snippet: Anti-bax, anti-bcl-2, anti-caspase-3, anti-occludin, anti-ZO-1, anti-TLR4, anti-NF-κB p65 and anti-phospho-p65 antibodies, HRP-labeled goat anti-rabbit IgG and HRP-labeled goat anti-mouse IgG were purchased from Bioss Biotech Co. Ltd. (Beijing, China).

Techniques: Expressing