Journal: Foods

Article Title: Ethanol Extract of Mao Jian Green Tea Attenuates Gastrointestinal Symptoms in a Rat Model of Irritable Bowel Syndrome with Constipation via the 5-hydroxytryptamine Signaling Pathway

doi: 10.3390/foods12051101

Figure Lengend Snippet: Western blotting analysis of TPH1 ( A ), TPH2 ( B ), SERT ( C ), 5-HT 3 receptors ( D ), 5-HT 4 receptors ( E ), CaM ( F ), and MLCK ( G ) in colonic tissues of each group. Capital and lowercase letters above the bar indicate the difference significance at the 0.01 or 0.05 levels, respectively. ( n = 8 for each group). NC: negative control group; MG: model group, MSP: mosapride group, MJGT_EE: Mao Jian Green Tea ethanol extract group; 5-HT: 5-hydroxytryptamine; TPH1: tryptophan hydroxylase 1; TPH2: tryptophan hydroxylase 2; SERT: serotonin transporter; CAM: calmodulin; MLCK: myosin light chain kinase; 5-HT 3 R: 5-HT 3 receptor; 5-HT 4 R: 5-HT 4 receptor.

Article Snippet: Second, primary antibodies against 5-hydroxytryptamine receptor 3 (5-HT 3 R; bs-2126R), 5-hydroxytryptamine 4 receptor (5-HT 4 R; bs-12054R), the serotonin transporter (SERT, bs-1893R), tryptophan hydroxylase 1 (TPH1; bs-1215R), tryptophan hydroxylase 2 (TPH2; bs-8729R), calmodulin (CaM; bs-3666R), or myosin light chain kinase (MLCK) (Bioss Biotechnology Co., Ltd., Beijing, China) were added (1:1000) to the membrane and incubated at 4 °C for 12 h. Next, the membrane was washed thrice with TBST buffer (T1081, Solarbio Science & Technology Co., Ltd., Beijing, China); then, secondary antibodies (HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) or HRP-conjugated Affinipure Goat Anti-Rabbit IgGs (H+L); 1:5000) were added to the membrane and incubated at room temperature for 1 h. Finally, at the end of the incubation, the membrane was again washed thrice with the TBST buffer, developed, exposed in a dark room, and imaged using a ChemiDoc MP Imaging System (Bio-Rad Laboratories Inc., Hercules, CA, USA).

Techniques: Western Blot, Negative Control