bs-11876R Search Results


tmem176b antibody  (Bioss)
94
Bioss tmem176b antibody
Antibody information in western blot
Tmem176b Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tmem176b antibody/product/Bioss
Average 94 stars, based on 1 article reviews
tmem176b antibody - by Bioz Stars, 2026-02
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Antibody information in western blot

Journal: World Journal of Gastrointestinal Oncology

Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

doi: 10.4251/wjgo.v17.i3.97673

Figure Lengend Snippet: Antibody information in western blot

Article Snippet: TMEM176B antibody , Bioss , bs-11876R , 1:1000.

Techniques: Western Blot

sh-Transmembrane protein 176B inhibits tumor metastasis in colon cancer mice. A: Fluorescence imaging was utilized to observe tumor metastasis in mice, with the total and average distribution of tumor fluorescence intensity being analyzed across different groups in mice; B and C: Colon tumor tissues in mice were harvested and subjected to Hematoxylin and eosin staining with magnifications of 200 × (scale bar 100 μm) and 400 × (scale bar 50 μm). For each group, n = 3. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B, sh-NC: shRNA-negative control.

Journal: World Journal of Gastrointestinal Oncology

Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

doi: 10.4251/wjgo.v17.i3.97673

Figure Lengend Snippet: sh-Transmembrane protein 176B inhibits tumor metastasis in colon cancer mice. A: Fluorescence imaging was utilized to observe tumor metastasis in mice, with the total and average distribution of tumor fluorescence intensity being analyzed across different groups in mice; B and C: Colon tumor tissues in mice were harvested and subjected to Hematoxylin and eosin staining with magnifications of 200 × (scale bar 100 μm) and 400 × (scale bar 50 μm). For each group, n = 3. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B, sh-NC: shRNA-negative control.

Article Snippet: TMEM176B antibody , Bioss , bs-11876R , 1:1000.

Techniques: Fluorescence, Imaging, Staining, shRNA, Negative Control

Short hairpin RNA targeting transmembrane protein 176B inhibits cancer promotion in colon cancer mice. A and B: The TUNEL-positive cells were stained in colon tumor tissues of mice with magnifications of 200 × (scale bar 100 μm) and the percentage of positive cells being calculated; C and D: Immunohistochemistry staining detected the positive expression of Ki67 in colon tumor tissues of mice, with magnifications of 200 × (scale bar 100 μm) and 400 × (scale bar 50 μm). Average optical density is the average optical density, calculated by analyzing the total optical density and area of the image using ImageJ. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; NC: Negative control; sh-NC: shRNA-negative control; AOD: Average optical density.

Journal: World Journal of Gastrointestinal Oncology

Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

doi: 10.4251/wjgo.v17.i3.97673

Figure Lengend Snippet: Short hairpin RNA targeting transmembrane protein 176B inhibits cancer promotion in colon cancer mice. A and B: The TUNEL-positive cells were stained in colon tumor tissues of mice with magnifications of 200 × (scale bar 100 μm) and the percentage of positive cells being calculated; C and D: Immunohistochemistry staining detected the positive expression of Ki67 in colon tumor tissues of mice, with magnifications of 200 × (scale bar 100 μm) and 400 × (scale bar 50 μm). Average optical density is the average optical density, calculated by analyzing the total optical density and area of the image using ImageJ. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; NC: Negative control; sh-NC: shRNA-negative control; AOD: Average optical density.

Article Snippet: TMEM176B antibody , Bioss , bs-11876R , 1:1000.

Techniques: shRNA, TUNEL Assay, Staining, Immunohistochemistry, Expressing, Negative Control

Short hairpin RNA targeting transmembrane protein 176B inhibits the epithelial-mesenchymal transition of colon cancer via the NLRP3 signaling pathway in mice. A and B: The positive expression and density of NLRP3 and E-cadherin were quantified using immunohistochemistry staining, with magnifications of 200 × (scale bar 100 μm) and 400 × (scale bar 50 μm); C and D: Flow cytometry was used to evaluate CD3 - NK1.1 + expression in colon cancer mice. n = 3 in each group. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; EMT: Epithelial-mesenchymal transition; AOD: Average optical density.

Journal: World Journal of Gastrointestinal Oncology

Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

doi: 10.4251/wjgo.v17.i3.97673

Figure Lengend Snippet: Short hairpin RNA targeting transmembrane protein 176B inhibits the epithelial-mesenchymal transition of colon cancer via the NLRP3 signaling pathway in mice. A and B: The positive expression and density of NLRP3 and E-cadherin were quantified using immunohistochemistry staining, with magnifications of 200 × (scale bar 100 μm) and 400 × (scale bar 50 μm); C and D: Flow cytometry was used to evaluate CD3 - NK1.1 + expression in colon cancer mice. n = 3 in each group. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; EMT: Epithelial-mesenchymal transition; AOD: Average optical density.

Article Snippet: TMEM176B antibody , Bioss , bs-11876R , 1:1000.

Techniques: shRNA, Expressing, Immunohistochemistry, Staining, Flow Cytometry, Negative Control

Short hairpin RNA targeting transmembrane protein 176B inhibits the epithelial-mesenchymal transition of colon cancer via the NLRP3 signaling pathway in mice. A: Enzyme-linked immunosorbent assay was used to quantify the expression levels of tumor necrosis factor-alpha and interleukin-18 (IL-18) in colon tumor tissues of mice. n = 3 in each group; B and C: Western blot analysis was used to measure the expression of NLRP3, ASC, caspase-1, IL-18, TMEM176B, p-p65, p65, MMP-9, TGF-β, N-cadherin, E-cadherin, Vimentin in colon tumor tissues of mice. n = 3 in each group. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; EMT: Epithelial-mesenchymal transition; ELISA: Enzyme-linked immunosorbent assay, TNF-α: Tumor necrosis factor-alpha; IL-18: Interleukin-18; TGF-β: Transforming growth factor-beta.

Journal: World Journal of Gastrointestinal Oncology

Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

doi: 10.4251/wjgo.v17.i3.97673

Figure Lengend Snippet: Short hairpin RNA targeting transmembrane protein 176B inhibits the epithelial-mesenchymal transition of colon cancer via the NLRP3 signaling pathway in mice. A: Enzyme-linked immunosorbent assay was used to quantify the expression levels of tumor necrosis factor-alpha and interleukin-18 (IL-18) in colon tumor tissues of mice. n = 3 in each group; B and C: Western blot analysis was used to measure the expression of NLRP3, ASC, caspase-1, IL-18, TMEM176B, p-p65, p65, MMP-9, TGF-β, N-cadherin, E-cadherin, Vimentin in colon tumor tissues of mice. n = 3 in each group. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; EMT: Epithelial-mesenchymal transition; ELISA: Enzyme-linked immunosorbent assay, TNF-α: Tumor necrosis factor-alpha; IL-18: Interleukin-18; TGF-β: Transforming growth factor-beta.

Article Snippet: TMEM176B antibody , Bioss , bs-11876R , 1:1000.

Techniques: shRNA, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Negative Control

Short hairpin RNA targeting transmembrane protein 176B increased inhibition rate of interleukin-2-exposed natural killer cells on CT26 cells. A: Flow cytometry was used to evaluate CD3 - NK1.1 + cell number and CD107 expression post interleukin-2 (IL-2) stimulation in natural killer (NK) cells. n = 3 in each group; B: Quantitative reverse transcription PCR was applied to assess the relative expression of TMEM176B and NLRP3 in CT26 cells. n = 3 in each group; C: MTT assay was used to determine the inhibition rate of IL-2-exposed NK cells on CT26 cells with TMEM176B or NLRP3 knockdown over 24, 48, and 72 hours. n = 3 in each group. a P < 0.05; b P < 0.01. NK: Natural killer; sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; IL-2: Interleukin-2.

Journal: World Journal of Gastrointestinal Oncology

Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

doi: 10.4251/wjgo.v17.i3.97673

Figure Lengend Snippet: Short hairpin RNA targeting transmembrane protein 176B increased inhibition rate of interleukin-2-exposed natural killer cells on CT26 cells. A: Flow cytometry was used to evaluate CD3 - NK1.1 + cell number and CD107 expression post interleukin-2 (IL-2) stimulation in natural killer (NK) cells. n = 3 in each group; B: Quantitative reverse transcription PCR was applied to assess the relative expression of TMEM176B and NLRP3 in CT26 cells. n = 3 in each group; C: MTT assay was used to determine the inhibition rate of IL-2-exposed NK cells on CT26 cells with TMEM176B or NLRP3 knockdown over 24, 48, and 72 hours. n = 3 in each group. a P < 0.05; b P < 0.01. NK: Natural killer; sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; IL-2: Interleukin-2.

Article Snippet: TMEM176B antibody , Bioss , bs-11876R , 1:1000.

Techniques: shRNA, Inhibition, Flow Cytometry, Expressing, Reverse Transcription, MTT Assay, Knockdown, Negative Control

Short hairpin RNA targeting transmembrane protein 176B inhibits migration and invasion in CT26 cells co-cultured with activated natural killer cells while promoting cell apoptosis. A-C: Wound healing and Transwell assays were used to assess migration distance, migration and invasion rates of CT26 cells co-cultured with interleukin-2 (IL-2)-exposed natural killer (NK) cells for 12 hours. With magnifications of 200 × (scale bar 100 μm) and 40 × (scale bar 400 μm). n = 3 in each group; D: Flow cytometry was used to analyze the apoptosis rate of CT26 cells co-cultured with IL-2-exposed NK cells. n = 3 in each group. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; NK: Natural killer; IL-2: Interleukin-2.

Journal: World Journal of Gastrointestinal Oncology

Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

doi: 10.4251/wjgo.v17.i3.97673

Figure Lengend Snippet: Short hairpin RNA targeting transmembrane protein 176B inhibits migration and invasion in CT26 cells co-cultured with activated natural killer cells while promoting cell apoptosis. A-C: Wound healing and Transwell assays were used to assess migration distance, migration and invasion rates of CT26 cells co-cultured with interleukin-2 (IL-2)-exposed natural killer (NK) cells for 12 hours. With magnifications of 200 × (scale bar 100 μm) and 40 × (scale bar 400 μm). n = 3 in each group; D: Flow cytometry was used to analyze the apoptosis rate of CT26 cells co-cultured with IL-2-exposed NK cells. n = 3 in each group. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; NK: Natural killer; IL-2: Interleukin-2.

Article Snippet: TMEM176B antibody , Bioss , bs-11876R , 1:1000.

Techniques: shRNA, Migration, Cell Culture, Flow Cytometry, Negative Control

Short hairpin RNA targeting transmembrane protein 176B inhibits the epithelial-mesenchymal transition process via the NLRP3 signaling pathway in CT26 cells. A: Enzyme-linked immunosorbent assay was used to quantify the expression of the interleukin-18 (IL-18) in CT26 cells; B and C: Western blot was used to measure the expression of the NLRP3, ASC, caspase-1, IL-18, TMEM176B, p-p65, p65, MMP-9, transforming growth factor-beta, N-cadherin, E-cadherin, Vimentin in CT26 cells. a P <0.05; b P <0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Overexpression-negative control; IL-18: Interleukin-18; TGF-β: Transforming growth factor-beta.

Journal: World Journal of Gastrointestinal Oncology

Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

doi: 10.4251/wjgo.v17.i3.97673

Figure Lengend Snippet: Short hairpin RNA targeting transmembrane protein 176B inhibits the epithelial-mesenchymal transition process via the NLRP3 signaling pathway in CT26 cells. A: Enzyme-linked immunosorbent assay was used to quantify the expression of the interleukin-18 (IL-18) in CT26 cells; B and C: Western blot was used to measure the expression of the NLRP3, ASC, caspase-1, IL-18, TMEM176B, p-p65, p65, MMP-9, transforming growth factor-beta, N-cadherin, E-cadherin, Vimentin in CT26 cells. a P <0.05; b P <0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Overexpression-negative control; IL-18: Interleukin-18; TGF-β: Transforming growth factor-beta.

Article Snippet: TMEM176B antibody , Bioss , bs-11876R , 1:1000.

Techniques: shRNA, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Over Expression, Negative Control