Journal: Molecular Biology Reports

Article Title: Assessment of atherosclerotic plaques in the rabbit abdominal aorta with interleukin-8 monoclonal antibody-targeted ultrasound microbubbles

doi: 10.1007/s11033-012-2382-5

Figure Lengend Snippet: Light micrograph of SonoVue microbubbles. a Non-conjugated SonoVue microbubbles. b IL-8 monoclonal antibody-conjugated SonoVue microbubbles (×100)

Article Snippet: After the addition of rabbit polyclonal antibody against IL-8 (Bioss, Beijing, China), the sections were incubated at room temperature for 60 min, washed with PBS, and stained using a MaxvisionTM2 HRP-Polymer anti-Mouse IHC Kit (Maxim Biotech Inc. Fuzhou, China).

Techniques:

Journal: Molecular Biology Reports

Article Title: Assessment of atherosclerotic plaques in the rabbit abdominal aorta with interleukin-8 monoclonal antibody-targeted ultrasound microbubbles

doi: 10.1007/s11033-012-2382-5

Figure Lengend Snippet: Light micrograph of the mixture of SonoVue microbubbles and goat anti-mouse serum. a Non-conjugated microbubbles (×100). b Non-conjugated microbubbles (×400). c IL-8 monoclonal antibody-conjugated microbubbles (×100). d IL-8 monoclonal antibody-conjugated microbubbles (×400)

Article Snippet: After the addition of rabbit polyclonal antibody against IL-8 (Bioss, Beijing, China), the sections were incubated at room temperature for 60 min, washed with PBS, and stained using a MaxvisionTM2 HRP-Polymer anti-Mouse IHC Kit (Maxim Biotech Inc. Fuzhou, China).

Techniques:

Journal: Molecular Biology Reports

Article Title: Assessment of atherosclerotic plaques in the rabbit abdominal aorta with interleukin-8 monoclonal antibody-targeted ultrasound microbubbles

doi: 10.1007/s11033-012-2382-5

Figure Lengend Snippet: Immunofluorescence image of IgG-FITC-stained SonoVue microbubbles. a No fluorescence was observed on the surface of non-conjugated SonoVue microbubbles. b Bright-green fluorescence can be observed on the surface of IL-8-conjugated SonoVue microbubbles (×400). (Color figure online)

Article Snippet: After the addition of rabbit polyclonal antibody against IL-8 (Bioss, Beijing, China), the sections were incubated at room temperature for 60 min, washed with PBS, and stained using a MaxvisionTM2 HRP-Polymer anti-Mouse IHC Kit (Maxim Biotech Inc. Fuzhou, China).

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: Molecular Biology Reports

Article Title: Assessment of atherosclerotic plaques in the rabbit abdominal aorta with interleukin-8 monoclonal antibody-targeted ultrasound microbubbles

doi: 10.1007/s11033-012-2382-5

Figure Lengend Snippet: Representative ultrasound images of the atherosclerotic plaques in the rabbit abdominal aorta. a Two-dimensional image showing a plaque ( large arrow ); a soft plaque was invisible ( small arrow ). b Contrast ultrasound imaging with normal microbubbles showing two plaques with stronger echo compared with the two-dimensional view. The enhancement of the echo was more apparent for the soft plaque ( small arrow ). c Contrast ultrasound imaging with IL-8-targeted microbubbles showing two plaques with a markedly stronger echo compared with normal contrast ultrasound

Article Snippet: After the addition of rabbit polyclonal antibody against IL-8 (Bioss, Beijing, China), the sections were incubated at room temperature for 60 min, washed with PBS, and stained using a MaxvisionTM2 HRP-Polymer anti-Mouse IHC Kit (Maxim Biotech Inc. Fuzhou, China).

Techniques: Imaging

Journal: Molecular Biology Reports

Article Title: Assessment of atherosclerotic plaques in the rabbit abdominal aorta with interleukin-8 monoclonal antibody-targeted ultrasound microbubbles

doi: 10.1007/s11033-012-2382-5

Figure Lengend Snippet: Sequential ultrasound images of IL-8-targeted imaging for an atherosclerotic plaque of the rabbit abdominal aorta. a Conventional two-dimensional view. b – g Sequential targeted CEU view: b 3.8 s post-contrast agent injection, c : 4.3 s post-contrast agent injection, d 4.9 s post-contrast agent injection; e 9.8 s post-contrast agent injection, f 68 s post-contrast agent injection, g 176 s post-contrast agent injection

Article Snippet: After the addition of rabbit polyclonal antibody against IL-8 (Bioss, Beijing, China), the sections were incubated at room temperature for 60 min, washed with PBS, and stained using a MaxvisionTM2 HRP-Polymer anti-Mouse IHC Kit (Maxim Biotech Inc. Fuzhou, China).

Techniques: Imaging, Injection

Journal: Molecular Biology Reports

Article Title: Assessment of atherosclerotic plaques in the rabbit abdominal aorta with interleukin-8 monoclonal antibody-targeted ultrasound microbubbles

doi: 10.1007/s11033-012-2382-5

Figure Lengend Snippet: The time-intensity curves of contrast ultrasound imaging with normal microbubbles ( a ) and IL-8-targeted microbubbles ( b ). The yellow , blue and red curves indicate the intensity of the lumen, intima and plaques, respectively. (Color figure online)

Article Snippet: After the addition of rabbit polyclonal antibody against IL-8 (Bioss, Beijing, China), the sections were incubated at room temperature for 60 min, washed with PBS, and stained using a MaxvisionTM2 HRP-Polymer anti-Mouse IHC Kit (Maxim Biotech Inc. Fuzhou, China).

Techniques: Imaging

Journal: Molecular Biology Reports

Article Title: Assessment of atherosclerotic plaques in the rabbit abdominal aorta with interleukin-8 monoclonal antibody-targeted ultrasound microbubbles

doi: 10.1007/s11033-012-2382-5

Figure Lengend Snippet: Immunohistochemical expression of IL-8 in atherosclerotic plaques in the rabbit abdominal aorta. Brown -stained area indicates positive IL-8 expression. ( a ×40; b ×200). (Color figure online)

Article Snippet: After the addition of rabbit polyclonal antibody against IL-8 (Bioss, Beijing, China), the sections were incubated at room temperature for 60 min, washed with PBS, and stained using a MaxvisionTM2 HRP-Polymer anti-Mouse IHC Kit (Maxim Biotech Inc. Fuzhou, China).

Techniques: Immunohistochemical staining, Expressing, Staining