bs-0730R-RBITC Search Results


fitc anti hspb1 antibody  (Bioss)
94
Bioss fitc anti hspb1 antibody
Fumagillin and DHA suppress cholesterol-induced low-grade inflammation by inhibiting membrane clustering of Src and relieving the mTORC1 circuitry. (A–H) BMMCs were treated with ethanol (Ctrl), cholesterol (Chol), Fumagillin (Fuma), or cholesterol + Fumagillin (Chol + Fuma) for 5 days. The expression levels of Src, SYK, p70S6K, STAT3, and STAT5 were examined by Western blotting and quantified after normalizing to β-Actin expression (A–F). Primed BMMCs were stained with anti-Src antibody and DAPI, and subcellular distribution of Src was examined by confocal microscopy (G). (H) Src aggregation was quantified using ImageJ by measuring the aggregate area normalized to total cell area (8 images/group). (I–S) BMMCs were treated with PBS (Ctrl), DHA alone, cholesterol + DMSO, or cholesterol + DHA for 5 d. The expression levels of Src, SYK, p70S6K, STAT3, and STAT5 were examined by Western blotting and quantified after normalizing to β-Actin expression (I–N). The levels of phosphorylated <t>HSPB1</t> at Ser15 (P-HSPB1) and total HSPB1 (T-HSPB1) were examined by Western blotting (O). The level of P-HSPB1 was quantified after normalizing to β-actin (P) or total HSPB1 (Q). Primed BMMCs were stained with anti-HSPB1 antibody and DAPI, and subcellular distribution of HSPB1 was examined by confocal microscopy. Scale bars = 5 μm. (R). HSPB1 aggregation was quantified using ImageJ by measuring the aggregate area normalized to total cell area (10 images/group) Statistical analysis was performed using 1-way ANOVA ( n =3–4 per group). Data represent mean ± standard deviation (SD) (* P < 0.05, ** P < 0.01, *** P < 0.001; NS, not significant).
Fitc Anti Hspb1 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bs-0730R-RBITC/HSP27+Polyclonal+Antibody%2C+FITC+Conjugated/pmc12929915-68-19-21
Average 94 stars, based on 1 article reviews
fitc anti hspb1 antibody - by Bioz Stars, 2026-11
94/100 stars
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hsp27 polyclonal antibody, rbitc conjugated  (Bioss)
N/A
Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is
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Image Search Results


Fumagillin and DHA suppress cholesterol-induced low-grade inflammation by inhibiting membrane clustering of Src and relieving the mTORC1 circuitry. (A–H) BMMCs were treated with ethanol (Ctrl), cholesterol (Chol), Fumagillin (Fuma), or cholesterol + Fumagillin (Chol + Fuma) for 5 days. The expression levels of Src, SYK, p70S6K, STAT3, and STAT5 were examined by Western blotting and quantified after normalizing to β-Actin expression (A–F). Primed BMMCs were stained with anti-Src antibody and DAPI, and subcellular distribution of Src was examined by confocal microscopy (G). (H) Src aggregation was quantified using ImageJ by measuring the aggregate area normalized to total cell area (8 images/group). (I–S) BMMCs were treated with PBS (Ctrl), DHA alone, cholesterol + DMSO, or cholesterol + DHA for 5 d. The expression levels of Src, SYK, p70S6K, STAT3, and STAT5 were examined by Western blotting and quantified after normalizing to β-Actin expression (I–N). The levels of phosphorylated HSPB1 at Ser15 (P-HSPB1) and total HSPB1 (T-HSPB1) were examined by Western blotting (O). The level of P-HSPB1 was quantified after normalizing to β-actin (P) or total HSPB1 (Q). Primed BMMCs were stained with anti-HSPB1 antibody and DAPI, and subcellular distribution of HSPB1 was examined by confocal microscopy. Scale bars = 5 μm. (R). HSPB1 aggregation was quantified using ImageJ by measuring the aggregate area normalized to total cell area (10 images/group) Statistical analysis was performed using 1-way ANOVA ( n =3–4 per group). Data represent mean ± standard deviation (SD) (* P < 0.05, ** P < 0.01, *** P < 0.001; NS, not significant).

Journal: ImmunoHorizons

Article Title: Sustained monocyte activation by persistent challenges with either oxLDL or free cholesterol and underlying mechanisms

doi: 10.1093/immhor/vlag005

Figure Lengend Snippet: Fumagillin and DHA suppress cholesterol-induced low-grade inflammation by inhibiting membrane clustering of Src and relieving the mTORC1 circuitry. (A–H) BMMCs were treated with ethanol (Ctrl), cholesterol (Chol), Fumagillin (Fuma), or cholesterol + Fumagillin (Chol + Fuma) for 5 days. The expression levels of Src, SYK, p70S6K, STAT3, and STAT5 were examined by Western blotting and quantified after normalizing to β-Actin expression (A–F). Primed BMMCs were stained with anti-Src antibody and DAPI, and subcellular distribution of Src was examined by confocal microscopy (G). (H) Src aggregation was quantified using ImageJ by measuring the aggregate area normalized to total cell area (8 images/group). (I–S) BMMCs were treated with PBS (Ctrl), DHA alone, cholesterol + DMSO, or cholesterol + DHA for 5 d. The expression levels of Src, SYK, p70S6K, STAT3, and STAT5 were examined by Western blotting and quantified after normalizing to β-Actin expression (I–N). The levels of phosphorylated HSPB1 at Ser15 (P-HSPB1) and total HSPB1 (T-HSPB1) were examined by Western blotting (O). The level of P-HSPB1 was quantified after normalizing to β-actin (P) or total HSPB1 (Q). Primed BMMCs were stained with anti-HSPB1 antibody and DAPI, and subcellular distribution of HSPB1 was examined by confocal microscopy. Scale bars = 5 μm. (R). HSPB1 aggregation was quantified using ImageJ by measuring the aggregate area normalized to total cell area (10 images/group) Statistical analysis was performed using 1-way ANOVA ( n =3–4 per group). Data represent mean ± standard deviation (SD) (* P < 0.05, ** P < 0.01, *** P < 0.001; NS, not significant).

Article Snippet: After blocking with 10% goat serum, the samples were stained with FITC-anti-Src antibody (Santa Cruz, no. sc-8056 FITC) or FITC-anti-HSPB1 antibody (Bioss, no. bs-0730R-FITC).

Techniques: Membrane, Expressing, Western Blot, Staining, Confocal Microscopy, Standard Deviation