Journal: Scientific Reports

Article Title: Proteomic analysis of CD29+ Müller cells reveals metabolic reprogramming in rabbit myopia model

doi: 10.1038/s41598-024-75637-1

Figure Lengend Snippet: Representation of proteomic analysis of Müller cells derived from the retina of myopia-induced rabbits. ( a ) Establishment of a myopia model in rabbits through form deprivation. The retina was segmented into central and peripheral regions based on the distance from the visual streak to the limbus. The central retina was defined as the area within 4 mm from the visual streak, while the peripheral retina included areas beyond this boundary. ( b ) Isolation of Müller cells from both the central and peripheral regions of the retina using magnetic-activated cell sorting (MACS) with CD29 antibody selection. ( c ) Subsequent proteomic analysis performed on the isolated Müller cells.

Article Snippet: For selective enrichment of Müller cells, CD29-FITC (1:50, bs-0486R-FITC, Bioss, Woburn, MA, USA) was added and incubated at 4 °C for another 30 min.

Techniques: Derivative Assay, Isolation, FACS, Selection

Journal: Scientific Reports

Article Title: Proteomic analysis of CD29+ Müller cells reveals metabolic reprogramming in rabbit myopia model

doi: 10.1038/s41598-024-75637-1

Figure Lengend Snippet: Validation of CD29+ cell identity as Müller cells. ( a ) Comparison of CD29+ and CD29- cells revealed that CD29+ cells exhibited larger and more elongated morphology, characteristic of Müller cells, as opposed to CD29- cells. The cytoplasm of CD29+ cells showed a significantly stronger CD29 immunofluorescence signal, indicating effective isolation. ( b ) Quantitative analysis showed a significant increase in mean CD29 immunofluorescence intensity in the retina of myopic rabbits compared to controls. ( c ) Immunofluorescence staining of CD29+ cells from both the central and peripheral retinal regions demonstrated co-expression of glial fibrillary acidic protein (GFAP) and glutamine synthetase (GLUL), confirming their identity as Müller cells.

Article Snippet: For selective enrichment of Müller cells, CD29-FITC (1:50, bs-0486R-FITC, Bioss, Woburn, MA, USA) was added and incubated at 4 °C for another 30 min.

Techniques: Comparison, Immunofluorescence, Isolation, Staining, Expressing

Journal: Scientific Reports

Article Title: Proteomic analysis of CD29+ Müller cells reveals metabolic reprogramming in rabbit myopia model

doi: 10.1038/s41598-024-75637-1

Figure Lengend Snippet: Proteomics analysis of CD29+ Müller cells in the myopic retina of rabbits. ( a ) Heatmap depicting unsupervised hierarchical clustering of protein expression profiles in CD29+ Müller cells, highlighting significant differences between the myopic and control groups. The analysis includes a Gene Ontology biological process breakdown using ClueGO to identify key signaling pathways. ( b ) Protein-protein interaction (PPI) network constructed using differentially expressed proteins ( p -value < 0.5), illustrating the interactions and potential regulatory mechanisms differentially regulated in the myopic retina. ( c ) Comprehensive protein-protein interaction (PPI) network incorporating proteins universally expressed in CD29+ Müller cells across both the central and peripheral regions of the retina.

Article Snippet: For selective enrichment of Müller cells, CD29-FITC (1:50, bs-0486R-FITC, Bioss, Woburn, MA, USA) was added and incubated at 4 °C for another 30 min.

Techniques: Expressing, Control, Construct

Journal: Scientific Reports

Article Title: Proteomic analysis of CD29+ Müller cells reveals metabolic reprogramming in rabbit myopia model

doi: 10.1038/s41598-024-75637-1

Figure Lengend Snippet: Differential expressed proteins in CD29+ Müller cells from myopia-induced central and peripheral retina (fold change > 1.2, p -value < 0.1).

Article Snippet: For selective enrichment of Müller cells, CD29-FITC (1:50, bs-0486R-FITC, Bioss, Woburn, MA, USA) was added and incubated at 4 °C for another 30 min.

Techniques: