bs-0315R Search Results


fgl2  (Bioss)
90
Bioss fgl2
Mean mRNA ± SEM expression of (A) leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (B) hairy/enhancer-of-split related with YRPW motif 1 (Hey1), (C) fibrinogen-like protein 2 <t>(Fgl2),</t> and (D) lymphotoxin (Ltb) across photoperiod groups: control LD, regressed SD, and recrudescing PT day 2 and week 1 (PTd2 and PTw1) groups. Columns with different letters indicate a statistically significant difference (P < 0.05) for the analysis, with differing letters indicating a statistical difference between groups indicated.
Fgl2, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mean mRNA ± SEM expression of (A) leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (B) hairy/enhancer-of-split related with YRPW motif 1 (Hey1), (C) fibrinogen-like protein 2 (Fgl2), and (D) lymphotoxin (Ltb) across photoperiod groups: control LD, regressed SD, and recrudescing PT day 2 and week 1 (PTd2 and PTw1) groups. Columns with different letters indicate a statistically significant difference (P < 0.05) for the analysis, with differing letters indicating a statistical difference between groups indicated.

Journal: Biology of Reproduction

Article Title: Transcriptome analysis during photostimulated recrudescence reveals distinct patterns of gene regulation in Siberian hamster ovaries

doi: 10.1093/biolre/ioz210

Figure Lengend Snippet: Mean mRNA ± SEM expression of (A) leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (B) hairy/enhancer-of-split related with YRPW motif 1 (Hey1), (C) fibrinogen-like protein 2 (Fgl2), and (D) lymphotoxin (Ltb) across photoperiod groups: control LD, regressed SD, and recrudescing PT day 2 and week 1 (PTd2 and PTw1) groups. Columns with different letters indicate a statistically significant difference (P < 0.05) for the analysis, with differing letters indicating a statistical difference between groups indicated.

Article Snippet: Endogenous peroxidases were blocked with 3% hydrogen peroxide in methanol, and the R.T.U Vectastain Universal Quick Kit (Vector Laboratories Burlingame, CA) was used as directed with empirically determined primary antibody concentrations for AQP9 (1:150 dilution; product no. bs-2060R; Bioss, Inc. Woburn, MA), COL4A1 (1:175 dilution; product no. bs-4595R; Bioss, Inc. Woburn, MA), FGL2 (1:175 dilution; product no. bs-0315R; Bioss, Inc. Woburn, MA), INHBB (1:500 dilution; product no. PA5–48128; Invitrogen Waltham, MA), Waltham, MA and TUBB4A (1:100 dilution; product no. MA1–19164; Invitrogen Waltham, MA).

Techniques: Expressing

Representative ovarian FGL2 protein immunodetection (red stain on purple/blue hematoxylin background) in (A) control LD, (B) regressed SD, and (C) recrudescing PT day 2 (PTd2) and (D) week 1 (PTw1) groups. Negative control: insets show no staining without primary antibody present. Average ± SEM relative (E) extent and (F) intensity of FGL2 immunostaining detected across the photoperiod groups. Panels with columns with different letters indicate a statistically significant difference (P < 0.05) for the analysis, with differing letters indicating a statistical difference between groups indicated; specific P-values are indicated on panels where the analysis yielded no significant difference (P > 0.05).

Journal: Biology of Reproduction

Article Title: Transcriptome analysis during photostimulated recrudescence reveals distinct patterns of gene regulation in Siberian hamster ovaries

doi: 10.1093/biolre/ioz210

Figure Lengend Snippet: Representative ovarian FGL2 protein immunodetection (red stain on purple/blue hematoxylin background) in (A) control LD, (B) regressed SD, and (C) recrudescing PT day 2 (PTd2) and (D) week 1 (PTw1) groups. Negative control: insets show no staining without primary antibody present. Average ± SEM relative (E) extent and (F) intensity of FGL2 immunostaining detected across the photoperiod groups. Panels with columns with different letters indicate a statistically significant difference (P < 0.05) for the analysis, with differing letters indicating a statistical difference between groups indicated; specific P-values are indicated on panels where the analysis yielded no significant difference (P > 0.05).

Article Snippet: Endogenous peroxidases were blocked with 3% hydrogen peroxide in methanol, and the R.T.U Vectastain Universal Quick Kit (Vector Laboratories Burlingame, CA) was used as directed with empirically determined primary antibody concentrations for AQP9 (1:150 dilution; product no. bs-2060R; Bioss, Inc. Woburn, MA), COL4A1 (1:175 dilution; product no. bs-4595R; Bioss, Inc. Woburn, MA), FGL2 (1:175 dilution; product no. bs-0315R; Bioss, Inc. Woburn, MA), INHBB (1:500 dilution; product no. PA5–48128; Invitrogen Waltham, MA), Waltham, MA and TUBB4A (1:100 dilution; product no. MA1–19164; Invitrogen Waltham, MA).

Techniques: Immunodetection, Staining, Negative Control, Immunostaining