bs-0293P-BF488 Search Results


91
Bioss antibodies conjugated to alexa fluor 488 donkey anti rabbit igg
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Antibodies Conjugated To Alexa Fluor 488 Donkey Anti Rabbit Igg, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies conjugated to alexa fluor 488 donkey anti rabbit igg/product/Bioss
Average 91 stars, based on 1 article reviews
antibodies conjugated to alexa fluor 488 donkey anti rabbit igg - by Bioz Stars, 2026-02
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94
Bioss goat antimouse igg alexa fluor 488
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Goat Antimouse Igg Alexa Fluor 488, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat antimouse igg alexa fluor 488/product/Bioss
Average 94 stars, based on 1 article reviews
goat antimouse igg alexa fluor 488 - by Bioz Stars, 2026-02
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94
Bioss goat anti rabbit igg immunoglobulin g alexa fluor 488 conjugate antibodies
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Goat Anti Rabbit Igg Immunoglobulin G Alexa Fluor 488 Conjugate Antibodies, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
goat anti rabbit igg immunoglobulin g alexa fluor 488 conjugate antibodies - by Bioz Stars, 2026-02
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94
Bioss rabbit igg conjugated to alexafluor488
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Rabbit Igg Conjugated To Alexafluor488, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg conjugated to alexafluor488/product/Bioss
Average 94 stars, based on 1 article reviews
rabbit igg conjugated to alexafluor488 - by Bioz Stars, 2026-02
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93
Bioss antibodies alexa fluor 488 goat anti rat igg
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Antibodies Alexa Fluor 488 Goat Anti Rat Igg, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
antibodies alexa fluor 488 goat anti rat igg - by Bioz Stars, 2026-02
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94
Bioss anti mouse secondary antibody
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Anti Mouse Secondary Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mouse secondary antibody/product/Bioss
Average 94 stars, based on 1 article reviews
anti mouse secondary antibody - by Bioz Stars, 2026-02
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91
Bioss alexa fluor 488 conjugated rat igg
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Alexa Fluor 488 Conjugated Rat Igg, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa fluor 488 conjugated rat igg/product/Bioss
Average 91 stars, based on 1 article reviews
alexa fluor 488 conjugated rat igg - by Bioz Stars, 2026-02
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90
Bioss alexa fluor 488 labeled donkey anti goat igg
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Alexa Fluor 488 Labeled Donkey Anti Goat Igg, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
alexa fluor 488 labeled donkey anti goat igg - by Bioz Stars, 2026-02
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90
Bioss alexa fluor 488 mouse anti rabbit igg
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Alexa Fluor 488 Mouse Anti Rabbit Igg, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
alexa fluor 488 mouse anti rabbit igg - by Bioz Stars, 2026-02
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90
Bioss procedure 2 goat antihuman antibody alexa 488 conjugate
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Procedure 2 Goat Antihuman Antibody Alexa 488 Conjugate, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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procedure 2 goat antihuman antibody alexa 488 conjugate - by Bioz Stars, 2026-02
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93
Bioss alexa fluor 488 conjugated rabbit anti human igg
Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa <t>Fluor</t> <t>488</t> donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.
Alexa Fluor 488 Conjugated Rabbit Anti Human Igg, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa fluor 488 conjugated rabbit anti human igg/product/Bioss
Average 93 stars, based on 1 article reviews
alexa fluor 488 conjugated rabbit anti human igg - by Bioz Stars, 2026-02
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N/A
Isotype controls are negative controls that are designed to measure the amount of non-specific background signal caused by the primary antibody. Isotype Control have no specificity for the target protein, yet retain all of the
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Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa Fluor 488 donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.

Journal: Frontiers in Immunology

Article Title: Enhanced Susceptibility of ADAP-Deficient Mice to Listeria monocytogenes Infection Is Associated With an Altered Phagocyte Phenotype and Function

doi: 10.3389/fimmu.2021.724855

Figure Lengend Snippet: Distinct changes in NET formation by neutrophils from ADAP-deficient mice in response to in vivo Listeria monocytogenes ( Lm ) infection. Wild type (▪) and ADAPko (▫) mice (age: 10–14 weeks) were infected i.v. with 2.5 × 10 4 CFU Lm (strain 10403S) and sacrificed at the indicated times post infection. Formalin-fixed tissue slices were stained with primary antibody (neutrophil elastase (NE) and histone H3) and subsequently with the secondary antibody (Alexa Fluor 488 donkey anti-rabbit IgG and goat anti-rat IgG Alexa Fluor 647) as well as DAPI for nuclei staining. Four pictures per slide (three different layers) at a magnification of ×200 were taken, and histones were counted using Image-Pro Plus 6 (double-blinded). Representative pictures show the overlay of the nuclei (colored in blue), histones (colored in red), and neutrophil elastase (colored in green) in (A) spleen and (B) liver tissues, whereas white arrows highlight the histones. Summary plots show the means of counted histones. Data are depicted as box and whiskers ± min to max for n = 4 individually analyzed mice per genotype out of one experiment. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post-hoc test (*p < 0.05). NET, neutrophil extracellular trap; ADAP, adhesion and degranulation-promoting adaptor protein; ADAPko, adhesion and degranulation-promoting adaptor protein knockout.

Article Snippet: The same procedure was followed for the secondary antibodies conjugated to Alexa Fluor 488 donkey anti-rabbit IgG (Bioss) and Alexa Fluor 647 goat anti-rat IgG (Thermo Fisher) with an incubation of 30 min at RT in the dark.

Techniques: In Vivo, Infection, Staining, Knock-Out