Journal: Infection and Immunity

Article Title: The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

doi: 10.1128/IAI.00901-18

Figure Lengend Snippet: Survival profile of NSG mice. Mice were inoculated intraperitoneally with either B. abortus S19 or B. abortus S19ΔvjbR at various doses (1 × 104, 1 × 105, and 1 × 106 CFU/mouse) or with PBS. Mice inoculated with PBS or the B. abortus S19ΔvjbR vaccine candidate survived (100%) longer than mice inoculated with B. abortus S19.

Article Snippet: Blocked sections were incubated with polyclonal rabbit anti- Brucella abortus primary antibody (1:4,000) (bs-2229R; Bioss Antibodies, USA) diluted in PBS (pH 7.4) for 60 min at room temperature.

Techniques:

Journal: Infection and Immunity

Article Title: The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

doi: 10.1128/IAI.00901-18

Figure Lengend Snippet: Temperature profiles of NSG mice inoculated with Brucella abortus. Mice were implanted with transponders, and body temperature was monitored every day over a period of 13 weeks following inoculation (i.p.) with B. abortus S19, the B. abortus S19ΔvjbR vaccine candidate, or PBS, as indicated. No significant changes in body temperature were observed (P > 0.05) over time in mice inoculated with B. abortus S19ΔvjbR or PBS. However, mice inoculated with different doses of B. abortus S19 demonstrated a gradual reduction in body temperature (hypothermia) that reached a significant level from week 7 onwards (***, P < 0.001). Doses are indicated such that S19-104, for example, indicates 1 × 104 CFU/mouse of B. abortus S19.

Article Snippet: Blocked sections were incubated with polyclonal rabbit anti- Brucella abortus primary antibody (1:4,000) (bs-2229R; Bioss Antibodies, USA) diluted in PBS (pH 7.4) for 60 min at room temperature.

Techniques:

Journal: Infection and Immunity

Article Title: The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

doi: 10.1128/IAI.00901-18

Figure Lengend Snippet: Body weight profiles of NSG mice inoculated with attenuated Brucella abortus. Body weight was monitored over a period of 13 weeks postinoculation with 1 × 104, 1 × 105, and 1 × 106 CFU/mouse of either B. abortus S19 or the B. abortus S19ΔvjbR vaccine candidate or with PBS, as indicated. Mice inoculated with 1 × 105 and 1 × 106 CFU/mouse of B. abortus S19 demonstrated significant weight loss by 13 weeks. *, P < 0.05.

Article Snippet: Blocked sections were incubated with polyclonal rabbit anti- Brucella abortus primary antibody (1:4,000) (bs-2229R; Bioss Antibodies, USA) diluted in PBS (pH 7.4) for 60 min at room temperature.

Techniques:

Journal: Infection and Immunity

Article Title: The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

doi: 10.1128/IAI.00901-18

Figure Lengend Snippet: Organ weight and bacterial colonization of NSG mice inoculated with Brucella. Mice inoculated (i.p.) with B. abortus S19 showed a significant increase in spleen weight compared with mice inoculated with the B. abortus S19ΔvjbR vaccine candidate (A), while there was no significant difference in liver weights in mice inoculated with Brucella or PBS (B). Mice inoculated with B. abortus S19ΔvjbR had significantly reduced bacterial burdens in spleen (C), liver (D), and lung (E) compared to those in the B. abortus S19-inoculated group. *, P < 0.05; ns, not significant.

Article Snippet: Blocked sections were incubated with polyclonal rabbit anti- Brucella abortus primary antibody (1:4,000) (bs-2229R; Bioss Antibodies, USA) diluted in PBS (pH 7.4) for 60 min at room temperature.

Techniques:

Journal: Infection and Immunity

Article Title: The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

doi: 10.1128/IAI.00901-18

Figure Lengend Snippet: Gross image and tail histopathological image from NSG mice inoculated with PBS, B. abortus S19ΔvjbR (1 × 106 CFU/mouse), and B. abortus S19 (1 × 106 CFU/mouse), as indicated. (A) Representative image of NSG mouse inoculated with B. abortus S19 demonstrates inflammation in tail vertebrae. The magnified tail region with or without artificial coloring clearly demonstrates the regions of inflammation or bone damage (boxed selections). (B) Representative images of bone histopathology at low and high magnifications. Magnified images at right correspond with the boxed sections in the left panel. Top and middle panels depict intact bone mass and cartilage with normal appearance. Infection with B. abortus S19 induced severe bone resorption and destruction of the intervertebral discs which were replaced by fibrous connective tissue (bottom panel). Magnified images in the bottom right panel show neutrophil and macrophage infiltration (arrows) and activation of osteoclasts (arrowhead).

Article Snippet: Blocked sections were incubated with polyclonal rabbit anti- Brucella abortus primary antibody (1:4,000) (bs-2229R; Bioss Antibodies, USA) diluted in PBS (pH 7.4) for 60 min at room temperature.

Techniques: Histopathology, Infection, Activation Assay

Journal: Infection and Immunity

Article Title: The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

doi: 10.1128/IAI.00901-18

Figure Lengend Snippet: Representative images of histopathology of spleen. (A) Representative bright-field image of spleen from immunocompetent mice (top) and from NSG mice inoculated with PBS, B. abortus S19ΔvjbR (1 × 106 CFU/mouse), and B. abortus S19 (1 × 106 CFU/mouse), as indicated. Images at right are magnifications of the boxed sections in the left panels. Mice infected with B. abortus S19 exhibited multifocal neutrophilic and histiocytic splenitis in contrast to mild neutrophilic and histiocytic splenitis in B. abortus S19ΔvjbR-inoculated mice. (B) Immunohistochemical localization of Brucella in mouse spleen (boxed).

Article Snippet: Blocked sections were incubated with polyclonal rabbit anti- Brucella abortus primary antibody (1:4,000) (bs-2229R; Bioss Antibodies, USA) diluted in PBS (pH 7.4) for 60 min at room temperature.

Techniques: Histopathology, Infection, Immunohistochemical staining

Journal: Infection and Immunity

Article Title: The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

doi: 10.1128/IAI.00901-18

Figure Lengend Snippet: Representative images of histopathology of liver. (A) Representative bright-field image of liver from NSG mice inoculated with PBS, B. abortus S19ΔvjbR (1 × 106 CFU/mouse), and B. abortus S19 (1 × 106 CFU/mouse), as indicated. Images at right are magnifications of the boxed sections in the left panel. Animals infected with B. abortus S19 exhibited neutrophilic and histiocytic infiltration. None of the NSG mice inoculated with B. abortus S19ΔvjbR or PBS showed remarkable lesions. (B) Immunohistochemical localization of Brucella in mouse liver (arrows).

Article Snippet: Blocked sections were incubated with polyclonal rabbit anti- Brucella abortus primary antibody (1:4,000) (bs-2229R; Bioss Antibodies, USA) diluted in PBS (pH 7.4) for 60 min at room temperature.

Techniques: Histopathology, Infection, Immunohistochemical staining

Journal: Infection and Immunity

Article Title: The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

doi: 10.1128/IAI.00901-18

Figure Lengend Snippet: Immunohistochemical, immunofluorescence, and fluorescence in situ hybridization (FISH) analysis of Brucella in paraffin sections of tail vertebrae. (A) Representative immunohistochemical images of tail vertebrae of NSG mice inoculated with PBS, B. abortus S19ΔvjbR (1 × 106 CFU/mouse) and B. abortus S19 (1 × 106 CFU/mouse). B. abortus S19-inoculated mice exhibited widely distributed Brucella antigens (brown) in the osteoarticular lesions, with the highest concentration in the osteoclasts (arrowhead). Only one mouse inoculated with 1 × 106 CFU/mouse of B. abortus S19ΔvjbR exhibited positive staining for Brucella (arrows). (B) Representative immunofluorescence images of tail vertebrae of NSG mice inoculated with PBS, B. abortus S19ΔvjbR (1 × 106 CFU/mouse), and B. abortus S19 (1 × 106 CFU/mouse). B. abortus S19-inoculated mice exhibited strong positive signal for Brucella antigens (green) that widely spread in the area of inflammation and osteoclasts (arrowheads), whereas NSG mice inoculated with B. abortus S19ΔvjbR exhibited a faint signal of Brucella antigens in few places (arrows). (C) Representative FISH image reveals hybridization of Brucella-specific Bru-996 Alexa Fluor-labeled DNA probe of the 16S rRNA gene with B. abortus S19 (red) in tail vertebral sections. Images at right are magnifications of the boxed sections in the left panel.

Article Snippet: Blocked sections were incubated with polyclonal rabbit anti- Brucella abortus primary antibody (1:4,000) (bs-2229R; Bioss Antibodies, USA) diluted in PBS (pH 7.4) for 60 min at room temperature.

Techniques: Immunohistochemical staining, Immunofluorescence, Fluorescence, In Situ Hybridization, Concentration Assay, Staining, Hybridization, Labeling

Journal: Infection and Immunity

Article Title: The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

doi: 10.1128/IAI.00901-18

Figure Lengend Snippet: Brucella colonization in osteoclasts. (A) Representative immunofluorescence image of tail vertebrae of B. abortus S19-inoculated mice (1 × 106 CFU/mouse) demonstrated colocalization (yellow) of Brucella antigen (green) with the osteoclast marker cathepsin (red signal). Images at right are magnifications of the boxed section in the left panel. (B) Representative confocal immunofluorescence images showing a large number of B. abortus S19 bacteria (green) inside osteoclasts. The fluorescence intensity plot profile in the right panel corresponds with the images in the left panel. (C) Z sectioning shows Brucella localization in different depths of the osteoclast. AU, arbitrary units. (D) Quantitative image analysis shows a significant decrease (***, P < 0.001) in Brucella colonization in the B. abortus S19ΔvjbR-inoculated group compared with that in the B. abortus S19-inoculated group, as reflected in total pixel intensity.

Article Snippet: Blocked sections were incubated with polyclonal rabbit anti- Brucella abortus primary antibody (1:4,000) (bs-2229R; Bioss Antibodies, USA) diluted in PBS (pH 7.4) for 60 min at room temperature.

Techniques: Immunofluorescence, Marker, Fluorescence

Journal: BMC Infectious Diseases

Article Title: Seroprevalence and risk factors of brucellosis among slaughtered indigenous cattle, abattoir personnel and pregnant women in Ngaoundéré, Cameroon

doi: 10.1186/s12879-018-3522-x

Figure Lengend Snippet: Brucellosis seropositivity among slaughtered cattle ( n = 590) and personnel ( n = 107) in Ngaoundéré municipal abattoir of Cameroon according to combined results of Rose Bengal Plate test and Enzyme Linked Immunosorbent Assay

Article Snippet: The commercial Brucella IgG ELISA kit (RE56841®, IBL International GMbH, Hamburg, Germany) for qualitative and quantitative determination of IgG antibodies against Brucella in human serum was performed according to the manufacturer’s instructions and essentially as described by Esmaeili et al. [ ].

Techniques: Enzyme-linked Immunosorbent Assay

Journal: BMC Infectious Diseases

Article Title: Seroprevalence and risk factors of brucellosis among slaughtered indigenous cattle, abattoir personnel and pregnant women in Ngaoundéré, Cameroon

doi: 10.1186/s12879-018-3522-x

Figure Lengend Snippet: Socio-demographic characteristics and brucellosis seroprevalence among personnel ( n = 107) of the Ngaoundéré municipal abattoir in Cameroon

Article Snippet: The commercial Brucella IgG ELISA kit (RE56841®, IBL International GMbH, Hamburg, Germany) for qualitative and quantitative determination of IgG antibodies against Brucella in human serum was performed according to the manufacturer’s instructions and essentially as described by Esmaeili et al. [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay

Journal: BMC Infectious Diseases

Article Title: Seroprevalence and risk factors of brucellosis among slaughtered indigenous cattle, abattoir personnel and pregnant women in Ngaoundéré, Cameroon

doi: 10.1186/s12879-018-3522-x

Figure Lengend Snippet: Brucellosis seropositivity among personnel of the Ngaoundéré municipal abattoir according to potential risk factors ( n = 107)

Article Snippet: The commercial Brucella IgG ELISA kit (RE56841®, IBL International GMbH, Hamburg, Germany) for qualitative and quantitative determination of IgG antibodies against Brucella in human serum was performed according to the manufacturer’s instructions and essentially as described by Esmaeili et al. [ ].

Techniques: Enzyme-linked Immunosorbent Assay