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  • 99
    Millipore brefeldin a
    Brefeldin A, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brefeldin a - by Bioz Stars, 2021-06
    99/100 stars
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    86
    Becton Dickinson brefeldin a
    Unprotected mice have higher regulatory immune responses compared to protected mice. Hepatic CD4 T cells (gated Foxp3 − ) and Foxp3 + CD25 + Treg cells of protected and unprotected ID immunized mice were analyzed for regulatory marker expression at day 7 after challenge under artesunate treatment. (A) Representative FACS plots of Foxp3 − gated CD4 T cells for intracellular IL-10 expression after 36 h culture of hepatic leukocytes with CSP and sporozoites and addition of PMA/ionomycin plus <t>brefeldin</t> A in the last 4 h, and for surface expression of CTLA-4 or GITR ex vivo . Graphs show a summary of (B) intracellular IL-10 expression, (C) surface CTLA-4 and (D) GITR expression of Foxp3 − CD4 T cells and Treg cells from 2 experiments with 8–14 mice per group. The dotted line indicates the mean frequency for protected IV immunized mice (N = 9–16). Significant difference by Mann-Whitney test is indicated by *p
    Brefeldin A, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brefeldin a - by Bioz Stars, 2021-06
    86/100 stars
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    99
    Thermo Fisher brefeldin a
    Unprotected mice have higher regulatory immune responses compared to protected mice. Hepatic CD4 T cells (gated Foxp3 − ) and Foxp3 + CD25 + Treg cells of protected and unprotected ID immunized mice were analyzed for regulatory marker expression at day 7 after challenge under artesunate treatment. (A) Representative FACS plots of Foxp3 − gated CD4 T cells for intracellular IL-10 expression after 36 h culture of hepatic leukocytes with CSP and sporozoites and addition of PMA/ionomycin plus <t>brefeldin</t> A in the last 4 h, and for surface expression of CTLA-4 or GITR ex vivo . Graphs show a summary of (B) intracellular IL-10 expression, (C) surface CTLA-4 and (D) GITR expression of Foxp3 − CD4 T cells and Treg cells from 2 experiments with 8–14 mice per group. The dotted line indicates the mean frequency for protected IV immunized mice (N = 9–16). Significant difference by Mann-Whitney test is indicated by *p
    Brefeldin A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brefeldin a - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    99
    BioLegend brefeldin a
    Unprotected mice have higher regulatory immune responses compared to protected mice. Hepatic CD4 T cells (gated Foxp3 − ) and Foxp3 + CD25 + Treg cells of protected and unprotected ID immunized mice were analyzed for regulatory marker expression at day 7 after challenge under artesunate treatment. (A) Representative FACS plots of Foxp3 − gated CD4 T cells for intracellular IL-10 expression after 36 h culture of hepatic leukocytes with CSP and sporozoites and addition of PMA/ionomycin plus <t>brefeldin</t> A in the last 4 h, and for surface expression of CTLA-4 or GITR ex vivo . Graphs show a summary of (B) intracellular IL-10 expression, (C) surface CTLA-4 and (D) GITR expression of Foxp3 − CD4 T cells and Treg cells from 2 experiments with 8–14 mice per group. The dotted line indicates the mean frequency for protected IV immunized mice (N = 9–16). Significant difference by Mann-Whitney test is indicated by *p
    Brefeldin A, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a/product/BioLegend
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brefeldin a - by Bioz Stars, 2021-06
    99/100 stars
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    N/A
    GEF inhibitor
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    N/A
    Brefeldin A BFA is a natural fungal metabolite which has been used extensively to study intracellular transport by vesicles or endosomes Early studies demonstrated that BFA reversibly interferes with protein
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    N/A
    A 10 mM 5 0 mg in 1 78 ml sterile filtered solution of the HDR homology directed repair enhancer Brefeldin A Cat No 1560 in anhydrous DMSO
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    Unprotected mice have higher regulatory immune responses compared to protected mice. Hepatic CD4 T cells (gated Foxp3 − ) and Foxp3 + CD25 + Treg cells of protected and unprotected ID immunized mice were analyzed for regulatory marker expression at day 7 after challenge under artesunate treatment. (A) Representative FACS plots of Foxp3 − gated CD4 T cells for intracellular IL-10 expression after 36 h culture of hepatic leukocytes with CSP and sporozoites and addition of PMA/ionomycin plus brefeldin A in the last 4 h, and for surface expression of CTLA-4 or GITR ex vivo . Graphs show a summary of (B) intracellular IL-10 expression, (C) surface CTLA-4 and (D) GITR expression of Foxp3 − CD4 T cells and Treg cells from 2 experiments with 8–14 mice per group. The dotted line indicates the mean frequency for protected IV immunized mice (N = 9–16). Significant difference by Mann-Whitney test is indicated by *p

    Journal: Scientific Reports

    Article Title: Protective immunity differs between routes of administration of attenuated malaria parasites independent of parasite liver load

    doi: 10.1038/s41598-017-10480-1

    Figure Lengend Snippet: Unprotected mice have higher regulatory immune responses compared to protected mice. Hepatic CD4 T cells (gated Foxp3 − ) and Foxp3 + CD25 + Treg cells of protected and unprotected ID immunized mice were analyzed for regulatory marker expression at day 7 after challenge under artesunate treatment. (A) Representative FACS plots of Foxp3 − gated CD4 T cells for intracellular IL-10 expression after 36 h culture of hepatic leukocytes with CSP and sporozoites and addition of PMA/ionomycin plus brefeldin A in the last 4 h, and for surface expression of CTLA-4 or GITR ex vivo . Graphs show a summary of (B) intracellular IL-10 expression, (C) surface CTLA-4 and (D) GITR expression of Foxp3 − CD4 T cells and Treg cells from 2 experiments with 8–14 mice per group. The dotted line indicates the mean frequency for protected IV immunized mice (N = 9–16). Significant difference by Mann-Whitney test is indicated by *p

    Article Snippet: For the detection of intracellular IL-10 it was required to culture cells with CSP peptide (10 µg/ml) plus P. yoelii sporozoites (Py wt-GFP-Luccon , 25,000/ml) for 36 h including PMA, ionomycin and brefeldin A in the last 4 h. For flow cytometric detection of surface-exposed CD107a, cells were cultured for 4 hours with 0.25% GolgiStop (BD Biosciences), 10 µg/ml brefeldin A and anti-mouse CD107a-AlexaFluor488 (eBioscience, Vienna, Austria).

    Techniques: Mouse Assay, Marker, Expressing, FACS, Ex Vivo, MANN-WHITNEY

    ID immunization induces stronger regulatory immune responses in liver compared to IV. Hepatic leukocytes were analyzed 7 days after IV or ID primary immunization or boost for regulatory marker expression by flow cytometry directly ex vivo ( A , B ) or after 36 h culture with CSP and sporozoites ( C – G ). (A) Number of Foxp3 + CD25 + Treg cells per liver. (B) Frequency of Treg cells within the CD4 + T cell population. (C) Intracellular IL-10 expression of Treg cells after addition of PMA, ionomycin and brefeldin A for 4 h to the culture. (D) Representative FACS plots of CD4 + Foxp3-negative T cells (CD4 T) in one IV or ID immunized and a naïve control mouse. (E,F) Summary of intracellular IL-10 expression of CD4 + Foxp3 − T cells after addition of PMA, ionomycin and brefeldin A expressed as frequencies ( E ) and cell number per liver ( F ). Representative FACS plots ( G ) and summary ( H ) of intracellular IL-10 expression in CD19 + gated B cells after addition of PMA, ionomycin and brefeldin A. Graphs show 1 representative out of 2 similar experiments ( C ) or a summary of 2 experiments with 8–10 mice per group. Significant difference by Mann-Whitney test is indicated by *p

    Journal: Scientific Reports

    Article Title: Protective immunity differs between routes of administration of attenuated malaria parasites independent of parasite liver load

    doi: 10.1038/s41598-017-10480-1

    Figure Lengend Snippet: ID immunization induces stronger regulatory immune responses in liver compared to IV. Hepatic leukocytes were analyzed 7 days after IV or ID primary immunization or boost for regulatory marker expression by flow cytometry directly ex vivo ( A , B ) or after 36 h culture with CSP and sporozoites ( C – G ). (A) Number of Foxp3 + CD25 + Treg cells per liver. (B) Frequency of Treg cells within the CD4 + T cell population. (C) Intracellular IL-10 expression of Treg cells after addition of PMA, ionomycin and brefeldin A for 4 h to the culture. (D) Representative FACS plots of CD4 + Foxp3-negative T cells (CD4 T) in one IV or ID immunized and a naïve control mouse. (E,F) Summary of intracellular IL-10 expression of CD4 + Foxp3 − T cells after addition of PMA, ionomycin and brefeldin A expressed as frequencies ( E ) and cell number per liver ( F ). Representative FACS plots ( G ) and summary ( H ) of intracellular IL-10 expression in CD19 + gated B cells after addition of PMA, ionomycin and brefeldin A. Graphs show 1 representative out of 2 similar experiments ( C ) or a summary of 2 experiments with 8–10 mice per group. Significant difference by Mann-Whitney test is indicated by *p

    Article Snippet: For the detection of intracellular IL-10 it was required to culture cells with CSP peptide (10 µg/ml) plus P. yoelii sporozoites (Py wt-GFP-Luccon , 25,000/ml) for 36 h including PMA, ionomycin and brefeldin A in the last 4 h. For flow cytometric detection of surface-exposed CD107a, cells were cultured for 4 hours with 0.25% GolgiStop (BD Biosciences), 10 µg/ml brefeldin A and anti-mouse CD107a-AlexaFluor488 (eBioscience, Vienna, Austria).

    Techniques: Marker, Expressing, Flow Cytometry, Cytometry, Ex Vivo, FACS, Mouse Assay, MANN-WHITNEY

    ID but not IV immunization increases regulatory immune responses in the skin-draining lymph node. Inguinal lymph node cells were analyzed 7 days after IV or ID primary immunization for regulatory marker and cytokine expression. Flow cytometry was performed after 36 h culture with CSP and sporozoites and addition of PMA, ionomycin and brefeldin A in the last 4 h ( A , B ), or directly ex vivo ( C ). Frequencies calculated relative to naïve control are given for (A) IFN-γ + CD4 T cells and CD8 T cells, (B) IL-10 + Foxp3 − CD4 T cells, IL-10 + Foxp3 + CD25 + Treg cells, IL-10 + CD19 + B cells, and (C) CTLA-4 + Foxp3 − CD4 T cells and CTLA-4 + Foxp3 + CD25 + Treg cells. Summary of 2 experiments with 10 mice per group. Significant difference by unpaired t-test is indicated by *p

    Journal: Scientific Reports

    Article Title: Protective immunity differs between routes of administration of attenuated malaria parasites independent of parasite liver load

    doi: 10.1038/s41598-017-10480-1

    Figure Lengend Snippet: ID but not IV immunization increases regulatory immune responses in the skin-draining lymph node. Inguinal lymph node cells were analyzed 7 days after IV or ID primary immunization for regulatory marker and cytokine expression. Flow cytometry was performed after 36 h culture with CSP and sporozoites and addition of PMA, ionomycin and brefeldin A in the last 4 h ( A , B ), or directly ex vivo ( C ). Frequencies calculated relative to naïve control are given for (A) IFN-γ + CD4 T cells and CD8 T cells, (B) IL-10 + Foxp3 − CD4 T cells, IL-10 + Foxp3 + CD25 + Treg cells, IL-10 + CD19 + B cells, and (C) CTLA-4 + Foxp3 − CD4 T cells and CTLA-4 + Foxp3 + CD25 + Treg cells. Summary of 2 experiments with 10 mice per group. Significant difference by unpaired t-test is indicated by *p

    Article Snippet: For the detection of intracellular IL-10 it was required to culture cells with CSP peptide (10 µg/ml) plus P. yoelii sporozoites (Py wt-GFP-Luccon , 25,000/ml) for 36 h including PMA, ionomycin and brefeldin A in the last 4 h. For flow cytometric detection of surface-exposed CD107a, cells were cultured for 4 hours with 0.25% GolgiStop (BD Biosciences), 10 µg/ml brefeldin A and anti-mouse CD107a-AlexaFluor488 (eBioscience, Vienna, Austria).

    Techniques: Marker, Expressing, Flow Cytometry, Cytometry, Ex Vivo, Mouse Assay

    Unprotected mice after ID immunization show reduced liver T cell effector responses compared to protected mice. ID immunized mice were challenged followed by artesunate treatment, and distinguished as protected (p, i.e. luciferase negative) or unprotected (unp, i.e. luciferase positive). Hepatic CD8 and CD4 T cells were analyzed at day 7 after challenge. (A) IFN-γ concentration in supernatant of total leukocytes after culture for 36 h with CSP and sporozoites as measured by ELISA. (B) Representative FACS plots of CD8 + gated T cells for intracellular expression of IFN-γ or TNF after culture for 4 h with CSP and brefeldin A, and for surface expression of CD107a after 4 h culture with CSP, brefeldin A and monensin. Numbers indicate the frequency of the gated cell population. Graphs show a summary of 2 experiments with 8–14 mice per group for frequency of IFN-γ ( C ), TNF ( D ), or CD107a ( E ) -expressing CD8 T cells and CD4 T cells. The dotted line indicates the mean cytokine or expression level for protected IV immunized mice (N = 9–16). Significant difference by Mann-Whitney test is indicated by *p

    Journal: Scientific Reports

    Article Title: Protective immunity differs between routes of administration of attenuated malaria parasites independent of parasite liver load

    doi: 10.1038/s41598-017-10480-1

    Figure Lengend Snippet: Unprotected mice after ID immunization show reduced liver T cell effector responses compared to protected mice. ID immunized mice were challenged followed by artesunate treatment, and distinguished as protected (p, i.e. luciferase negative) or unprotected (unp, i.e. luciferase positive). Hepatic CD8 and CD4 T cells were analyzed at day 7 after challenge. (A) IFN-γ concentration in supernatant of total leukocytes after culture for 36 h with CSP and sporozoites as measured by ELISA. (B) Representative FACS plots of CD8 + gated T cells for intracellular expression of IFN-γ or TNF after culture for 4 h with CSP and brefeldin A, and for surface expression of CD107a after 4 h culture with CSP, brefeldin A and monensin. Numbers indicate the frequency of the gated cell population. Graphs show a summary of 2 experiments with 8–14 mice per group for frequency of IFN-γ ( C ), TNF ( D ), or CD107a ( E ) -expressing CD8 T cells and CD4 T cells. The dotted line indicates the mean cytokine or expression level for protected IV immunized mice (N = 9–16). Significant difference by Mann-Whitney test is indicated by *p

    Article Snippet: For the detection of intracellular IL-10 it was required to culture cells with CSP peptide (10 µg/ml) plus P. yoelii sporozoites (Py wt-GFP-Luccon , 25,000/ml) for 36 h including PMA, ionomycin and brefeldin A in the last 4 h. For flow cytometric detection of surface-exposed CD107a, cells were cultured for 4 hours with 0.25% GolgiStop (BD Biosciences), 10 µg/ml brefeldin A and anti-mouse CD107a-AlexaFluor488 (eBioscience, Vienna, Austria).

    Techniques: Mouse Assay, Luciferase, Concentration Assay, Enzyme-linked Immunosorbent Assay, FACS, Expressing, MANN-WHITNEY

    CD8 dendritic cell subset frequencies are smaller after ID compared to IV immunization. Dendritic cell (DC) subsets in liver were analyzed by flow cytometry at 7 days after primary or boost immunization via IV or ID route. (A) MHCII + CD11c hi/int DC were gated for CD11c int CD64 hi monocyte-derived DC (mo-DC) and CD11c hi CD64 low conventional DC (cDC) after excluding F4/80-expressing macrophages. Representative FACS plots of both DC subsets for CD8 and intracellular IL-12p40 expression in mice 7 days after IV or ID boost or in naïve control mice are shown. Numbers indicate the frequency of the gated cell population. (B) Frequencies of DC subsets within total leukocytes. (C) Number of CD8-expressing DC per liver. (D) Frequencies of CD8-expressing DC within the mo-DC or cDC subset. (E) Intracellular IL-12p40 expression of DC subsets after culture of leukocytes with brefeldin A for 4 h. Summary of 2 experiments with 8–10 mice per group. Significant difference by Mann-Whitney test is indicated by *p

    Journal: Scientific Reports

    Article Title: Protective immunity differs between routes of administration of attenuated malaria parasites independent of parasite liver load

    doi: 10.1038/s41598-017-10480-1

    Figure Lengend Snippet: CD8 dendritic cell subset frequencies are smaller after ID compared to IV immunization. Dendritic cell (DC) subsets in liver were analyzed by flow cytometry at 7 days after primary or boost immunization via IV or ID route. (A) MHCII + CD11c hi/int DC were gated for CD11c int CD64 hi monocyte-derived DC (mo-DC) and CD11c hi CD64 low conventional DC (cDC) after excluding F4/80-expressing macrophages. Representative FACS plots of both DC subsets for CD8 and intracellular IL-12p40 expression in mice 7 days after IV or ID boost or in naïve control mice are shown. Numbers indicate the frequency of the gated cell population. (B) Frequencies of DC subsets within total leukocytes. (C) Number of CD8-expressing DC per liver. (D) Frequencies of CD8-expressing DC within the mo-DC or cDC subset. (E) Intracellular IL-12p40 expression of DC subsets after culture of leukocytes with brefeldin A for 4 h. Summary of 2 experiments with 8–10 mice per group. Significant difference by Mann-Whitney test is indicated by *p

    Article Snippet: For the detection of intracellular IL-10 it was required to culture cells with CSP peptide (10 µg/ml) plus P. yoelii sporozoites (Py wt-GFP-Luccon , 25,000/ml) for 36 h including PMA, ionomycin and brefeldin A in the last 4 h. For flow cytometric detection of surface-exposed CD107a, cells were cultured for 4 hours with 0.25% GolgiStop (BD Biosciences), 10 µg/ml brefeldin A and anti-mouse CD107a-AlexaFluor488 (eBioscience, Vienna, Austria).

    Techniques: Flow Cytometry, Cytometry, Derivative Assay, Expressing, FACS, Mouse Assay, MANN-WHITNEY