breeding Search Results


speed congenics  (Transnetyx)
95
Transnetyx speed congenics
Speed Congenics, supplied by Transnetyx, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/10__3390_slash_genes17030255-52-16-29?v=Transnetyx
Average 95 stars, based on 1 article reviews
speed congenics - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
b linens atcc 9174  (ATCC)
93
ATCC b linens atcc 9174
B Linens Atcc 9174, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/pmc00525268-167-4-6?v=ATCC
Average 93 stars, based on 1 article reviews
b linens atcc 9174 - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
breeding colonies  (Jackson Laboratory)
86
Jackson Laboratory breeding colonies
Breeding Colonies, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/bio_rxiv__2025__09__15__676371-184-0-4?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
breeding colonies - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
resource source identifier ptgs2  (Jackson Laboratory)
86
Jackson Laboratory resource source identifier ptgs2
Figure 2. <t>Ptgs2-expressing</t> lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.
Resource Source Identifier Ptgs2, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/pm35908547-802-18-23?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
resource source identifier ptgs2 - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
breeding stocks  (Jackson Laboratory)
86
Jackson Laboratory breeding stocks
Figure 2. <t>Ptgs2-expressing</t> lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.
Breeding Stocks, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/10__1096_slash_fj__202505064r-32-0-5?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
breeding stocks - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
rodent breeding  (Envigo)
93
Envigo rodent breeding
Figure 2. <t>Ptgs2-expressing</t> lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.
Rodent Breeding, supplied by Envigo, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/pm38507407-234-10-12?v=Envigo
Average 93 stars, based on 1 article reviews
rodent breeding - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
s ship gfp transgenes  (Transnetyx)
94
Transnetyx s ship gfp transgenes
Figure 2. <t>Ptgs2-expressing</t> lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.
S Ship Gfp Transgenes, supplied by Transnetyx, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/pm30564749-169-6-11?v=Transnetyx
Average 94 stars, based on 1 article reviews
s ship gfp transgenes - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
70 linens atcc 9172  (ATCC)
91
ATCC 70 linens atcc 9172
Figure 2. <t>Ptgs2-expressing</t> lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.
70 Linens Atcc 9172, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/pm11849329-106-13-15?v=ATCC
Average 91 stars, based on 1 article reviews
70 linens atcc 9172 - by Bioz Stars, 2026-07
91/100 stars
  Buy from Supplier
znso4  (ATCC)
90
ATCC znso4
Figure 2. <t>Ptgs2-expressing</t> lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.
Znso4, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/us07455851-194-165-34?v=ATCC
Average 90 stars, based on 1 article reviews
znso4 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
brevibacterium linens 8377  (ATCC)
90
ATCC brevibacterium linens 8377
Figure 2. <t>Ptgs2-expressing</t> lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.
Brevibacterium Linens 8377, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/pmc00106727-12-0-4?v=ATCC
Average 90 stars, based on 1 article reviews
brevibacterium linens 8377 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
chem biol 10 5  (DSMZ)
92
DSMZ chem biol 10 5
Figure 2. <t>Ptgs2-expressing</t> lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.
Chem Biol 10 5, supplied by DSMZ, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/us07132257-27-4-18?v=DSMZ
Average 92 stars, based on 1 article reviews
chem biol 10 5 - by Bioz Stars, 2026-07
92/100 stars
  Buy from Supplier
protocatechuate dioxygenasewas prepared frombrevibacterium fuscum  (ATCC)
91
ATCC protocatechuate dioxygenasewas prepared frombrevibacterium fuscum
Figure 2. <t>Ptgs2-expressing</t> lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.
Protocatechuate Dioxygenasewas Prepared Frombrevibacterium Fuscum, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breeding/10__1074_slash_jbc__m609374200-32-0-5?v=ATCC
Average 91 stars, based on 1 article reviews
protocatechuate dioxygenasewas prepared frombrevibacterium fuscum - by Bioz Stars, 2026-07
91/100 stars
  Buy from Supplier

Image Search Results


Figure 2. Ptgs2-expressing lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.

Journal: Immunity

Article Title: Lung fibroblasts facilitate pre-metastatic niche formation by remodeling the local immune microenvironment.

doi: 10.1016/j.immuni.2022.07.001

Figure Lengend Snippet: Figure 2. Ptgs2-expressing lung fibroblasts modulate the lung resident immune microenvironment at the steady state (A) Quantification of Ptgs2 expression in different organs isolated from naïve Ptgs2Luc mice (n=6). (B) Analysis of PTGS2 expression in human normal tissue microarray data (GSE7307). (C-D) Ptgs2 expression and PGE2 production was measured in the indicated lung tissue cells (C) or different tissue-derived fibroblasts (D) isolated from naïve mice (n=4-6). MG, mammary gland.

Article Snippet: A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript REAGENT or RESOURCE SOURCE IDENTIFIER Ptgs2−/−: B6;129S-Ptgs2tm1Jed/J The Jackson Laboratory JAX: 002476 Ptgs2Luc: B6;129S4-Ptgs2tm2.1Hahe/J The Jackson Laboratory JAX: 030853 Ptgs2flox/flox: B6;129S4-Ptgs2tm1Hahe/J The Jackson Laboratory JAX: 030785 PdgfraCre: C57BL/6-Tg(Pdgfra-cre)1Clc/J The Jackson Laboratory JAX: 013148 PdgfraCre; Ptgs2flox/flox This paper N/A FVB/N-Tg(MMTV-PyVT)634Mul/J The Jackson Laboratory JAX: 002374 MMTV-PyMT: B6.FVB-Tg(MMTV-PyVT)634Mul/LellJ The Jackson Laboratory JAX: 022974 B6 Cd45.1 (B6.SJL-Ptprca Pepcb/BoyJ) The Jackson Laboratory JAX: 002014 PdgfaCre; Ptgs2flox/flox; MMTV This paper N/A Il1r1−/−: B6.129S7-Il1r1tm1Imx/J The Jackson Laboratory JAX: 003245 CD140aEGFP: B6.129S4-Pdgfratm11(EGFP)Sor/J The Jackson Laboratory JAX: 007669 Oligonucleotides See Table S1 for primer sequences Recombinant DNA pLenti CMV Puro LUC (w168-1) (Campeau et al., 2009) Addgene#17477 pLV-mCherry Tsoulfas lab Addgene #36084 pcDNA3-OVA (Diebold et al., 2001) Addgene #64599 Software and algorithms BD FACSDiva software BD Biosciences https://www.bdbiosciences.com/ FlowJo BD Biosciences https://flowjo.com R (version 4.0.2) R Core Team (2020) https://www.r-project.org/ Seurat (version 3.2.2) Satija Lab https://satijalab.org/seurat/ limma (version 3.46.0) (Ritchie et al., 2015) https://bioconductor.org/ packages/release/bioc/html/ limma.html Metascape (Zhou et al., 2019) https://metascape.org/ DoRothEA (version 1.1.2) (Holland et al., 2020) https://saezlab.github.io/dorothea/ Ingenuity Pathway Analysis QIAGEN https:// www.qiagenbioinformatics.com/ products/ingenuity-pathwayanalysis/ GraphPad Prism (version 8.2.1) GraphPad Software https://www.graphpad.com/ Biorender Biorender https://biorender.com/ ImageJ (1.53c) ImageJ https://imagej.nih.gov/ij/ Other Immunity.

Techniques: Expressing, Isolation, Microarray, Derivative Assay

Figure 3. Identification of Ptgs2-expressing fibroblasts by scRNA-seq (A) Workflow depicts isolation of CD140a+ lung fibroblasts from naïve CD140aEGFP mice for scRNA-seq. (B) t-SNE plots (left) and feature plots (right) showing the Ptgs2hi fibroblasts (cluster 0) among lung CD140a+ fibroblasts. (C) Schematic showing the PGE2 synthesis pathway (left), and violin plots (right) showing the expression levels of the indicated genes across each cluster. (D) Heatmap showing the expression of the top-rated marker genes across each cluster.

Journal: Immunity

Article Title: Lung fibroblasts facilitate pre-metastatic niche formation by remodeling the local immune microenvironment.

doi: 10.1016/j.immuni.2022.07.001

Figure Lengend Snippet: Figure 3. Identification of Ptgs2-expressing fibroblasts by scRNA-seq (A) Workflow depicts isolation of CD140a+ lung fibroblasts from naïve CD140aEGFP mice for scRNA-seq. (B) t-SNE plots (left) and feature plots (right) showing the Ptgs2hi fibroblasts (cluster 0) among lung CD140a+ fibroblasts. (C) Schematic showing the PGE2 synthesis pathway (left), and violin plots (right) showing the expression levels of the indicated genes across each cluster. (D) Heatmap showing the expression of the top-rated marker genes across each cluster.

Article Snippet: A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript REAGENT or RESOURCE SOURCE IDENTIFIER Ptgs2−/−: B6;129S-Ptgs2tm1Jed/J The Jackson Laboratory JAX: 002476 Ptgs2Luc: B6;129S4-Ptgs2tm2.1Hahe/J The Jackson Laboratory JAX: 030853 Ptgs2flox/flox: B6;129S4-Ptgs2tm1Hahe/J The Jackson Laboratory JAX: 030785 PdgfraCre: C57BL/6-Tg(Pdgfra-cre)1Clc/J The Jackson Laboratory JAX: 013148 PdgfraCre; Ptgs2flox/flox This paper N/A FVB/N-Tg(MMTV-PyVT)634Mul/J The Jackson Laboratory JAX: 002374 MMTV-PyMT: B6.FVB-Tg(MMTV-PyVT)634Mul/LellJ The Jackson Laboratory JAX: 022974 B6 Cd45.1 (B6.SJL-Ptprca Pepcb/BoyJ) The Jackson Laboratory JAX: 002014 PdgfaCre; Ptgs2flox/flox; MMTV This paper N/A Il1r1−/−: B6.129S7-Il1r1tm1Imx/J The Jackson Laboratory JAX: 003245 CD140aEGFP: B6.129S4-Pdgfratm11(EGFP)Sor/J The Jackson Laboratory JAX: 007669 Oligonucleotides See Table S1 for primer sequences Recombinant DNA pLenti CMV Puro LUC (w168-1) (Campeau et al., 2009) Addgene#17477 pLV-mCherry Tsoulfas lab Addgene #36084 pcDNA3-OVA (Diebold et al., 2001) Addgene #64599 Software and algorithms BD FACSDiva software BD Biosciences https://www.bdbiosciences.com/ FlowJo BD Biosciences https://flowjo.com R (version 4.0.2) R Core Team (2020) https://www.r-project.org/ Seurat (version 3.2.2) Satija Lab https://satijalab.org/seurat/ limma (version 3.46.0) (Ritchie et al., 2015) https://bioconductor.org/ packages/release/bioc/html/ limma.html Metascape (Zhou et al., 2019) https://metascape.org/ DoRothEA (version 1.1.2) (Holland et al., 2020) https://saezlab.github.io/dorothea/ Ingenuity Pathway Analysis QIAGEN https:// www.qiagenbioinformatics.com/ products/ingenuity-pathwayanalysis/ GraphPad Prism (version 8.2.1) GraphPad Software https://www.graphpad.com/ Biorender Biorender https://biorender.com/ ImageJ (1.53c) ImageJ https://imagej.nih.gov/ij/ Other Immunity.

Techniques: Expressing, Isolation, Marker

Figure 5. IL-1β reinforces the phenotype of Ptgs2hi lung fibroblasts (A) Ptgs2 expression (left), PGE2 production (middle), and frequency of COX-2+ cells (right) was measured in lung CD140a+ fibroblasts from naïve, AT3 and AT3-gcsf tumor- bearing mice (n=3-6). (B) Expression of the indicated genes in ex vivo cultured WT and Il1r−/− lung fibroblasts upon stimulation by vehicle or IL-1β (n=3). (C) COX-2 protein level in ex vivo cultured naïve mouse-derived lung fibroblasts stimulated with vehicle or IL-1β.

Journal: Immunity

Article Title: Lung fibroblasts facilitate pre-metastatic niche formation by remodeling the local immune microenvironment.

doi: 10.1016/j.immuni.2022.07.001

Figure Lengend Snippet: Figure 5. IL-1β reinforces the phenotype of Ptgs2hi lung fibroblasts (A) Ptgs2 expression (left), PGE2 production (middle), and frequency of COX-2+ cells (right) was measured in lung CD140a+ fibroblasts from naïve, AT3 and AT3-gcsf tumor- bearing mice (n=3-6). (B) Expression of the indicated genes in ex vivo cultured WT and Il1r−/− lung fibroblasts upon stimulation by vehicle or IL-1β (n=3). (C) COX-2 protein level in ex vivo cultured naïve mouse-derived lung fibroblasts stimulated with vehicle or IL-1β.

Article Snippet: A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript REAGENT or RESOURCE SOURCE IDENTIFIER Ptgs2−/−: B6;129S-Ptgs2tm1Jed/J The Jackson Laboratory JAX: 002476 Ptgs2Luc: B6;129S4-Ptgs2tm2.1Hahe/J The Jackson Laboratory JAX: 030853 Ptgs2flox/flox: B6;129S4-Ptgs2tm1Hahe/J The Jackson Laboratory JAX: 030785 PdgfraCre: C57BL/6-Tg(Pdgfra-cre)1Clc/J The Jackson Laboratory JAX: 013148 PdgfraCre; Ptgs2flox/flox This paper N/A FVB/N-Tg(MMTV-PyVT)634Mul/J The Jackson Laboratory JAX: 002374 MMTV-PyMT: B6.FVB-Tg(MMTV-PyVT)634Mul/LellJ The Jackson Laboratory JAX: 022974 B6 Cd45.1 (B6.SJL-Ptprca Pepcb/BoyJ) The Jackson Laboratory JAX: 002014 PdgfaCre; Ptgs2flox/flox; MMTV This paper N/A Il1r1−/−: B6.129S7-Il1r1tm1Imx/J The Jackson Laboratory JAX: 003245 CD140aEGFP: B6.129S4-Pdgfratm11(EGFP)Sor/J The Jackson Laboratory JAX: 007669 Oligonucleotides See Table S1 for primer sequences Recombinant DNA pLenti CMV Puro LUC (w168-1) (Campeau et al., 2009) Addgene#17477 pLV-mCherry Tsoulfas lab Addgene #36084 pcDNA3-OVA (Diebold et al., 2001) Addgene #64599 Software and algorithms BD FACSDiva software BD Biosciences https://www.bdbiosciences.com/ FlowJo BD Biosciences https://flowjo.com R (version 4.0.2) R Core Team (2020) https://www.r-project.org/ Seurat (version 3.2.2) Satija Lab https://satijalab.org/seurat/ limma (version 3.46.0) (Ritchie et al., 2015) https://bioconductor.org/ packages/release/bioc/html/ limma.html Metascape (Zhou et al., 2019) https://metascape.org/ DoRothEA (version 1.1.2) (Holland et al., 2020) https://saezlab.github.io/dorothea/ Ingenuity Pathway Analysis QIAGEN https:// www.qiagenbioinformatics.com/ products/ingenuity-pathwayanalysis/ GraphPad Prism (version 8.2.1) GraphPad Software https://www.graphpad.com/ Biorender Biorender https://biorender.com/ ImageJ (1.53c) ImageJ https://imagej.nih.gov/ij/ Other Immunity.

Techniques: Expressing, Ex Vivo, Cell Culture, Derivative Assay

Figure 6. Genetic ablation of Ptgs2 in CD140a+ fibroblasts mitigates lung metastasis of breast cancer (A) As depicted in the schematic (left), lung metastatic colonization in WT or Ptgs2ΔFb

Journal: Immunity

Article Title: Lung fibroblasts facilitate pre-metastatic niche formation by remodeling the local immune microenvironment.

doi: 10.1016/j.immuni.2022.07.001

Figure Lengend Snippet: Figure 6. Genetic ablation of Ptgs2 in CD140a+ fibroblasts mitigates lung metastasis of breast cancer (A) As depicted in the schematic (left), lung metastatic colonization in WT or Ptgs2ΔFb

Article Snippet: A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript REAGENT or RESOURCE SOURCE IDENTIFIER Ptgs2−/−: B6;129S-Ptgs2tm1Jed/J The Jackson Laboratory JAX: 002476 Ptgs2Luc: B6;129S4-Ptgs2tm2.1Hahe/J The Jackson Laboratory JAX: 030853 Ptgs2flox/flox: B6;129S4-Ptgs2tm1Hahe/J The Jackson Laboratory JAX: 030785 PdgfraCre: C57BL/6-Tg(Pdgfra-cre)1Clc/J The Jackson Laboratory JAX: 013148 PdgfraCre; Ptgs2flox/flox This paper N/A FVB/N-Tg(MMTV-PyVT)634Mul/J The Jackson Laboratory JAX: 002374 MMTV-PyMT: B6.FVB-Tg(MMTV-PyVT)634Mul/LellJ The Jackson Laboratory JAX: 022974 B6 Cd45.1 (B6.SJL-Ptprca Pepcb/BoyJ) The Jackson Laboratory JAX: 002014 PdgfaCre; Ptgs2flox/flox; MMTV This paper N/A Il1r1−/−: B6.129S7-Il1r1tm1Imx/J The Jackson Laboratory JAX: 003245 CD140aEGFP: B6.129S4-Pdgfratm11(EGFP)Sor/J The Jackson Laboratory JAX: 007669 Oligonucleotides See Table S1 for primer sequences Recombinant DNA pLenti CMV Puro LUC (w168-1) (Campeau et al., 2009) Addgene#17477 pLV-mCherry Tsoulfas lab Addgene #36084 pcDNA3-OVA (Diebold et al., 2001) Addgene #64599 Software and algorithms BD FACSDiva software BD Biosciences https://www.bdbiosciences.com/ FlowJo BD Biosciences https://flowjo.com R (version 4.0.2) R Core Team (2020) https://www.r-project.org/ Seurat (version 3.2.2) Satija Lab https://satijalab.org/seurat/ limma (version 3.46.0) (Ritchie et al., 2015) https://bioconductor.org/ packages/release/bioc/html/ limma.html Metascape (Zhou et al., 2019) https://metascape.org/ DoRothEA (version 1.1.2) (Holland et al., 2020) https://saezlab.github.io/dorothea/ Ingenuity Pathway Analysis QIAGEN https:// www.qiagenbioinformatics.com/ products/ingenuity-pathwayanalysis/ GraphPad Prism (version 8.2.1) GraphPad Software https://www.graphpad.com/ Biorender Biorender https://biorender.com/ ImageJ (1.53c) ImageJ https://imagej.nih.gov/ij/ Other Immunity.

Techniques: