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Image Search Results
Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Article Title: SNORA28 Promotes Proliferation and Radioresistance in Colorectal Cancer Cells through the STAT3 Pathway by Increasing H3K9 Acetylation in the LIFR Promoter.
doi: 10.1002/advs.202405332
Figure Lengend Snippet: Figure 6. SNORA28 recruits BRD4 to increase H3K9 acetylation in the LIFR promoter. A) The subcellular localization of SNORA28 in HT29 cells was analyzed using qRT-PCR. B) Primers were designed to target three different sites in the LIFR promoter region. C–D) CUT&RUN-qPCR assay for assess- ment of H3K9ac levels in the LIFR promoter after SNORA28 knockdown (C) and overexpression (D). E) CUT&RUN-qPCR assay for detecting BRD4 levels in LIFR promoter after SNORA28 overexpression. F–H) qRT-PCR analysis of the mRNA levels of SNORA28 (F), BRD4 (G), and LIFR (H) in HT29 cells
Article Snippet: These included antibodies against JAK1 (Cell Signaling Technology; # 29 261, 1:1000), JAK2 (Cell Signaling Technology; # 3230, 1:1000), STAT3 (Cell Signaling Technology; # 9139, 1:1000), p-STAT3 (Cell Signaling Technology; # 9145, 1:500), SOCS1 (Abcam; # ab280886, 1:1000), OSMR (Santa Cruz Biotechnology; # sc-271695, 1:100), LIFR (Santa Cruz Biotechnology; # sc-515337, 1:100),
Techniques: Quantitative RT-PCR, Knockdown, Over Expression