bp single-end sequencing Search Results


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  • 91
    Illumina Inc bp single end sequences
    Bp Single End Sequences, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp single end sequences/product/Illumina Inc
    Average 91 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    bp single end sequences - by Bioz Stars, 2020-02
    91/100 stars
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    83
    Illumina Inc bp single end sequencing reads
    Bp Single End Sequencing Reads, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 83/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp single end sequencing reads/product/Illumina Inc
    Average 83 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    bp single end sequencing reads - by Bioz Stars, 2020-02
    83/100 stars
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    75
    Illumina Inc 50 bp single end sequences
    50 Bp Single End Sequences, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 75/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/50 bp single end sequences/product/Illumina Inc
    Average 75 stars, based on 46 article reviews
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    50 bp single end sequences - by Bioz Stars, 2020-02
    75/100 stars
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    95
    Solexa bp single end sequencing
    Bp Single End Sequencing, supplied by Solexa, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp single end sequencing/product/Solexa
    Average 95 stars, based on 20 article reviews
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    bp single end sequencing - by Bioz Stars, 2020-02
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    98
    Illumina Inc bp single end rna sequencing
    Bp Single End Rna Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp single end rna sequencing/product/Illumina Inc
    Average 98 stars, based on 53 article reviews
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    bp single end rna sequencing - by Bioz Stars, 2020-02
    98/100 stars
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    80
    Illumina Inc 125 bp single end sequencing reagent kit
    125 Bp Single End Sequencing Reagent Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/125 bp single end sequencing reagent kit/product/Illumina Inc
    Average 80 stars, based on 8 article reviews
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    125 bp single end sequencing reagent kit - by Bioz Stars, 2020-02
    80/100 stars
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    87
    Illumina Inc 125 bp single end cycle sequencing kit
    125 Bp Single End Cycle Sequencing Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 87/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/125 bp single end cycle sequencing kit/product/Illumina Inc
    Average 87 stars, based on 12 article reviews
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    125 bp single end cycle sequencing kit - by Bioz Stars, 2020-02
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    80
    Illumina Inc 51 bp single end sequencing
    51 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 80/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/51 bp single end sequencing/product/Illumina Inc
    Average 80 stars, based on 13 article reviews
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    51 bp single end sequencing - by Bioz Stars, 2020-02
    80/100 stars
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    99
    Illumina Inc 50 bp single end sequencing
    50 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/50 bp single end sequencing/product/Illumina Inc
    Average 99 stars, based on 173 article reviews
    Price from $9.99 to $1999.99
    50 bp single end sequencing - by Bioz Stars, 2020-02
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    99
    Illumina Inc 100 bp single end sequencing
    A pooled screen for functional validation of CLD. a The screening strategy in SW480 cells. In brief, a pool of mutant SW480 cells harboring 12,471 sgRNA designs against 408 genes was generated by lentiviral infection and antibiotic selection. Fourteen million cells per condition were then treated with PBS (control) or recombinant TRAIL (treatment) for a total of 12 days. Subsequently, the genomic DNA of the samples was extracted and sgRNA composition analyzed by next-generation sequencing ( NGS ). b Comparison of sgRNA sequence counts between two biological replicates demonstrates high reproducibility (Pearson correlation coefficient ~0.79). c Distribution of sgRNAs targeting positive pathway regulators ( CASP8 , CASP3 , FADD , BAX , BID , TNFRSF10A , TNFRSF10B ) in red , negative regulators ( XIAP , BCL2L1 ) in blue , and random, non-targeting sgRNAs in orange between TRAIL ( y-axis ) and PBS ( x-axis ) treated cell populations. d Scatter plot showing relative enrichment of genes ( y-axis ) with their corresponding p value ( x-axis ). Positive regulators are plotted in red , negative regulators in blue , and random, non-targeting sgRNAs in orange. P values were calculated by Wilcoxon rank sum test between 30 sgRNAs of one gene and 200 random, non-targeting sgRNAs. Log 2 fold change is calculated as median log 2 ratio between normalized sgRNA count of TRAIL- over PBS-treated populations. The vertical line marks a p value of 0.05. e – g Median normalized fold change of all sgRNAs targeting three essential TRAIL pathway components. A total of <t>100</t> sgRNAs are depicted for each gene. Enriched sgRNAs are colored in red , depleted sgRNAs in grey . The dashed line represents the median fold change of all sgRNAs of the corresponding gene
    100 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 bp single end sequencing/product/Illumina Inc
    Average 99 stars, based on 194 article reviews
    Price from $9.99 to $1999.99
    100 bp single end sequencing - by Bioz Stars, 2020-02
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    86
    Illumina Inc 80 bp single end sequencing
    A pooled screen for functional validation of CLD. a The screening strategy in SW480 cells. In brief, a pool of mutant SW480 cells harboring 12,471 sgRNA designs against 408 genes was generated by lentiviral infection and antibiotic selection. Fourteen million cells per condition were then treated with PBS (control) or recombinant TRAIL (treatment) for a total of 12 days. Subsequently, the genomic DNA of the samples was extracted and sgRNA composition analyzed by next-generation sequencing ( NGS ). b Comparison of sgRNA sequence counts between two biological replicates demonstrates high reproducibility (Pearson correlation coefficient ~0.79). c Distribution of sgRNAs targeting positive pathway regulators ( CASP8 , CASP3 , FADD , BAX , BID , TNFRSF10A , TNFRSF10B ) in red , negative regulators ( XIAP , BCL2L1 ) in blue , and random, non-targeting sgRNAs in orange between TRAIL ( y-axis ) and PBS ( x-axis ) treated cell populations. d Scatter plot showing relative enrichment of genes ( y-axis ) with their corresponding p value ( x-axis ). Positive regulators are plotted in red , negative regulators in blue , and random, non-targeting sgRNAs in orange. P values were calculated by Wilcoxon rank sum test between 30 sgRNAs of one gene and 200 random, non-targeting sgRNAs. Log 2 fold change is calculated as median log 2 ratio between normalized sgRNA count of TRAIL- over PBS-treated populations. The vertical line marks a p value of 0.05. e – g Median normalized fold change of all sgRNAs targeting three essential TRAIL pathway components. A total of <t>100</t> sgRNAs are depicted for each gene. Enriched sgRNAs are colored in red , depleted sgRNAs in grey . The dashed line represents the median fold change of all sgRNAs of the corresponding gene
    80 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/80 bp single end sequencing/product/Illumina Inc
    Average 86 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    80 bp single end sequencing - by Bioz Stars, 2020-02
    86/100 stars
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    91
    Illumina Inc 150 bp single end sequencing
    A pooled screen for functional validation of CLD. a The screening strategy in SW480 cells. In brief, a pool of mutant SW480 cells harboring 12,471 sgRNA designs against 408 genes was generated by lentiviral infection and antibiotic selection. Fourteen million cells per condition were then treated with PBS (control) or recombinant TRAIL (treatment) for a total of 12 days. Subsequently, the genomic DNA of the samples was extracted and sgRNA composition analyzed by next-generation sequencing ( NGS ). b Comparison of sgRNA sequence counts between two biological replicates demonstrates high reproducibility (Pearson correlation coefficient ~0.79). c Distribution of sgRNAs targeting positive pathway regulators ( CASP8 , CASP3 , FADD , BAX , BID , TNFRSF10A , TNFRSF10B ) in red , negative regulators ( XIAP , BCL2L1 ) in blue , and random, non-targeting sgRNAs in orange between TRAIL ( y-axis ) and PBS ( x-axis ) treated cell populations. d Scatter plot showing relative enrichment of genes ( y-axis ) with their corresponding p value ( x-axis ). Positive regulators are plotted in red , negative regulators in blue , and random, non-targeting sgRNAs in orange. P values were calculated by Wilcoxon rank sum test between 30 sgRNAs of one gene and 200 random, non-targeting sgRNAs. Log 2 fold change is calculated as median log 2 ratio between normalized sgRNA count of TRAIL- over PBS-treated populations. The vertical line marks a p value of 0.05. e – g Median normalized fold change of all sgRNAs targeting three essential TRAIL pathway components. A total of <t>100</t> sgRNAs are depicted for each gene. Enriched sgRNAs are colored in red , depleted sgRNAs in grey . The dashed line represents the median fold change of all sgRNAs of the corresponding gene
    150 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/150 bp single end sequencing/product/Illumina Inc
    Average 91 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    150 bp single end sequencing - by Bioz Stars, 2020-02
    91/100 stars
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    81
    Solexa 160 bp single end sequencing
    A pooled screen for functional validation of CLD. a The screening strategy in SW480 cells. In brief, a pool of mutant SW480 cells harboring 12,471 sgRNA designs against 408 genes was generated by lentiviral infection and antibiotic selection. Fourteen million cells per condition were then treated with PBS (control) or recombinant TRAIL (treatment) for a total of 12 days. Subsequently, the genomic DNA of the samples was extracted and sgRNA composition analyzed by next-generation sequencing ( NGS ). b Comparison of sgRNA sequence counts between two biological replicates demonstrates high reproducibility (Pearson correlation coefficient ~0.79). c Distribution of sgRNAs targeting positive pathway regulators ( CASP8 , CASP3 , FADD , BAX , BID , TNFRSF10A , TNFRSF10B ) in red , negative regulators ( XIAP , BCL2L1 ) in blue , and random, non-targeting sgRNAs in orange between TRAIL ( y-axis ) and PBS ( x-axis ) treated cell populations. d Scatter plot showing relative enrichment of genes ( y-axis ) with their corresponding p value ( x-axis ). Positive regulators are plotted in red , negative regulators in blue , and random, non-targeting sgRNAs in orange. P values were calculated by Wilcoxon rank sum test between 30 sgRNAs of one gene and 200 random, non-targeting sgRNAs. Log 2 fold change is calculated as median log 2 ratio between normalized sgRNA count of TRAIL- over PBS-treated populations. The vertical line marks a p value of 0.05. e – g Median normalized fold change of all sgRNAs targeting three essential TRAIL pathway components. A total of <t>100</t> sgRNAs are depicted for each gene. Enriched sgRNAs are colored in red , depleted sgRNAs in grey . The dashed line represents the median fold change of all sgRNAs of the corresponding gene
    160 Bp Single End Sequencing, supplied by Solexa, used in various techniques. Bioz Stars score: 81/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/160 bp single end sequencing/product/Solexa
    Average 81 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    160 bp single end sequencing - by Bioz Stars, 2020-02
    81/100 stars
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    78
    Illumina Inc 40 bp single end sequencing
    A pooled screen for functional validation of CLD. a The screening strategy in SW480 cells. In brief, a pool of mutant SW480 cells harboring 12,471 sgRNA designs against 408 genes was generated by lentiviral infection and antibiotic selection. Fourteen million cells per condition were then treated with PBS (control) or recombinant TRAIL (treatment) for a total of 12 days. Subsequently, the genomic DNA of the samples was extracted and sgRNA composition analyzed by next-generation sequencing ( NGS ). b Comparison of sgRNA sequence counts between two biological replicates demonstrates high reproducibility (Pearson correlation coefficient ~0.79). c Distribution of sgRNAs targeting positive pathway regulators ( CASP8 , CASP3 , FADD , BAX , BID , TNFRSF10A , TNFRSF10B ) in red , negative regulators ( XIAP , BCL2L1 ) in blue , and random, non-targeting sgRNAs in orange between TRAIL ( y-axis ) and PBS ( x-axis ) treated cell populations. d Scatter plot showing relative enrichment of genes ( y-axis ) with their corresponding p value ( x-axis ). Positive regulators are plotted in red , negative regulators in blue , and random, non-targeting sgRNAs in orange. P values were calculated by Wilcoxon rank sum test between 30 sgRNAs of one gene and 200 random, non-targeting sgRNAs. Log 2 fold change is calculated as median log 2 ratio between normalized sgRNA count of TRAIL- over PBS-treated populations. The vertical line marks a p value of 0.05. e – g Median normalized fold change of all sgRNAs targeting three essential TRAIL pathway components. A total of <t>100</t> sgRNAs are depicted for each gene. Enriched sgRNAs are colored in red , depleted sgRNAs in grey . The dashed line represents the median fold change of all sgRNAs of the corresponding gene
    40 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/40 bp single end sequencing/product/Illumina Inc
    Average 78 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    40 bp single end sequencing - by Bioz Stars, 2020-02
    78/100 stars
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    77
    Illumina Inc 400 bp single end sequencing
    A pooled screen for functional validation of CLD. a The screening strategy in SW480 cells. In brief, a pool of mutant SW480 cells harboring 12,471 sgRNA designs against 408 genes was generated by lentiviral infection and antibiotic selection. Fourteen million cells per condition were then treated with PBS (control) or recombinant TRAIL (treatment) for a total of 12 days. Subsequently, the genomic DNA of the samples was extracted and sgRNA composition analyzed by next-generation sequencing ( NGS ). b Comparison of sgRNA sequence counts between two biological replicates demonstrates high reproducibility (Pearson correlation coefficient ~0.79). c Distribution of sgRNAs targeting positive pathway regulators ( CASP8 , CASP3 , FADD , BAX , BID , TNFRSF10A , TNFRSF10B ) in red , negative regulators ( XIAP , BCL2L1 ) in blue , and random, non-targeting sgRNAs in orange between TRAIL ( y-axis ) and PBS ( x-axis ) treated cell populations. d Scatter plot showing relative enrichment of genes ( y-axis ) with their corresponding p value ( x-axis ). Positive regulators are plotted in red , negative regulators in blue , and random, non-targeting sgRNAs in orange. P values were calculated by Wilcoxon rank sum test between 30 sgRNAs of one gene and 200 random, non-targeting sgRNAs. Log 2 fold change is calculated as median log 2 ratio between normalized sgRNA count of TRAIL- over PBS-treated populations. The vertical line marks a p value of 0.05. e – g Median normalized fold change of all sgRNAs targeting three essential TRAIL pathway components. A total of <t>100</t> sgRNAs are depicted for each gene. Enriched sgRNAs are colored in red , depleted sgRNAs in grey . The dashed line represents the median fold change of all sgRNAs of the corresponding gene
    400 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/400 bp single end sequencing/product/Illumina Inc
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    400 bp single end sequencing - by Bioz Stars, 2020-02
    77/100 stars
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    79
    Illumina Inc hiseq 100 bp single end sequencing
    A pooled screen for functional validation of CLD. a The screening strategy in SW480 cells. In brief, a pool of mutant SW480 cells harboring 12,471 sgRNA designs against 408 genes was generated by lentiviral infection and antibiotic selection. Fourteen million cells per condition were then treated with PBS (control) or recombinant TRAIL (treatment) for a total of 12 days. Subsequently, the genomic DNA of the samples was extracted and sgRNA composition analyzed by next-generation sequencing ( NGS ). b Comparison of sgRNA sequence counts between two biological replicates demonstrates high reproducibility (Pearson correlation coefficient ~0.79). c Distribution of sgRNAs targeting positive pathway regulators ( CASP8 , CASP3 , FADD , BAX , BID , TNFRSF10A , TNFRSF10B ) in red , negative regulators ( XIAP , BCL2L1 ) in blue , and random, non-targeting sgRNAs in orange between TRAIL ( y-axis ) and PBS ( x-axis ) treated cell populations. d Scatter plot showing relative enrichment of genes ( y-axis ) with their corresponding p value ( x-axis ). Positive regulators are plotted in red , negative regulators in blue , and random, non-targeting sgRNAs in orange. P values were calculated by Wilcoxon rank sum test between 30 sgRNAs of one gene and 200 random, non-targeting sgRNAs. Log 2 fold change is calculated as median log 2 ratio between normalized sgRNA count of TRAIL- over PBS-treated populations. The vertical line marks a p value of 0.05. e – g Median normalized fold change of all sgRNAs targeting three essential TRAIL pathway components. A total of <t>100</t> sgRNAs are depicted for each gene. Enriched sgRNAs are colored in red , depleted sgRNAs in grey . The dashed line represents the median fold change of all sgRNAs of the corresponding gene
    Hiseq 100 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 100 bp single end sequencing/product/Illumina Inc
    Average 79 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    hiseq 100 bp single end sequencing - by Bioz Stars, 2020-02
    79/100 stars
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    83
    Solexa 75 bp single end sequencing
    A pooled screen for functional validation of CLD. a The screening strategy in SW480 cells. In brief, a pool of mutant SW480 cells harboring 12,471 sgRNA designs against 408 genes was generated by lentiviral infection and antibiotic selection. Fourteen million cells per condition were then treated with PBS (control) or recombinant TRAIL (treatment) for a total of 12 days. Subsequently, the genomic DNA of the samples was extracted and sgRNA composition analyzed by next-generation sequencing ( NGS ). b Comparison of sgRNA sequence counts between two biological replicates demonstrates high reproducibility (Pearson correlation coefficient ~0.79). c Distribution of sgRNAs targeting positive pathway regulators ( CASP8 , CASP3 , FADD , BAX , BID , TNFRSF10A , TNFRSF10B ) in red , negative regulators ( XIAP , BCL2L1 ) in blue , and random, non-targeting sgRNAs in orange between TRAIL ( y-axis ) and PBS ( x-axis ) treated cell populations. d Scatter plot showing relative enrichment of genes ( y-axis ) with their corresponding p value ( x-axis ). Positive regulators are plotted in red , negative regulators in blue , and random, non-targeting sgRNAs in orange. P values were calculated by Wilcoxon rank sum test between 30 sgRNAs of one gene and 200 random, non-targeting sgRNAs. Log 2 fold change is calculated as median log 2 ratio between normalized sgRNA count of TRAIL- over PBS-treated populations. The vertical line marks a p value of 0.05. e – g Median normalized fold change of all sgRNAs targeting three essential TRAIL pathway components. A total of <t>100</t> sgRNAs are depicted for each gene. Enriched sgRNAs are colored in red , depleted sgRNAs in grey . The dashed line represents the median fold change of all sgRNAs of the corresponding gene
    75 Bp Single End Sequencing, supplied by Solexa, used in various techniques. Bioz Stars score: 83/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/75 bp single end sequencing/product/Solexa
    Average 83 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    75 bp single end sequencing - by Bioz Stars, 2020-02
    83/100 stars
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    88
    Illumina Inc 36 bp single end sequencing
    Barcode lentiviral vector, sequencing and analysis workflow. (a) The 20 bp DNA barcode was cloned into the non-coding region of a SIN (self-inactivating) lentiviral vector upstream of a UBC-eGFP cassette. The P5 <t>Illumina</t> sequencing adapter sequence was integrated next to the barcode, and the P7 adapter was added during the PCR amplification step (primer positions shown). (b) This PCR results in a 250 bp fragment that includes a 4 bp indexing tag to allow pooling of multiple samples into a single lane of a flow cell, in addition to the 20 bp random barcode sequence, and flanked on either side by eight 'anchor' bases, which act as markers to identify true barcode sequences within the sequencing data. Finally, the fragments contain a spacer of approximately 90 bp and the second (P7) Illumina adapter for sequencing. Integrating the adapter into the barcode vector allows for single-end 36 bp (short) sequencing reads in which the barcode end is always sequenced. (c) Data analysis workflow.
    36 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/36 bp single end sequencing/product/Illumina Inc
    Average 88 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    36 bp single end sequencing - by Bioz Stars, 2020-02
    88/100 stars
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    99
    Illumina Inc 75 bp single end sequencing
    Barcode lentiviral vector, sequencing and analysis workflow. (a) The 20 bp DNA barcode was cloned into the non-coding region of a SIN (self-inactivating) lentiviral vector upstream of a UBC-eGFP cassette. The P5 <t>Illumina</t> sequencing adapter sequence was integrated next to the barcode, and the P7 adapter was added during the PCR amplification step (primer positions shown). (b) This PCR results in a 250 bp fragment that includes a 4 bp indexing tag to allow pooling of multiple samples into a single lane of a flow cell, in addition to the 20 bp random barcode sequence, and flanked on either side by eight 'anchor' bases, which act as markers to identify true barcode sequences within the sequencing data. Finally, the fragments contain a spacer of approximately 90 bp and the second (P7) Illumina adapter for sequencing. Integrating the adapter into the barcode vector allows for single-end 36 bp (short) sequencing reads in which the barcode end is always sequenced. (c) Data analysis workflow.
    75 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/75 bp single end sequencing/product/Illumina Inc
    Average 99 stars, based on 73 article reviews
    Price from $9.99 to $1999.99
    75 bp single end sequencing - by Bioz Stars, 2020-02
    99/100 stars
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    79
    Illumina Inc multiplexed 50 bp single end sequencing
    Barcode lentiviral vector, sequencing and analysis workflow. (a) The 20 bp DNA barcode was cloned into the non-coding region of a SIN (self-inactivating) lentiviral vector upstream of a UBC-eGFP cassette. The P5 <t>Illumina</t> sequencing adapter sequence was integrated next to the barcode, and the P7 adapter was added during the PCR amplification step (primer positions shown). (b) This PCR results in a 250 bp fragment that includes a 4 bp indexing tag to allow pooling of multiple samples into a single lane of a flow cell, in addition to the 20 bp random barcode sequence, and flanked on either side by eight 'anchor' bases, which act as markers to identify true barcode sequences within the sequencing data. Finally, the fragments contain a spacer of approximately 90 bp and the second (P7) Illumina adapter for sequencing. Integrating the adapter into the barcode vector allows for single-end 36 bp (short) sequencing reads in which the barcode end is always sequenced. (c) Data analysis workflow.
    Multiplexed 50 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplexed 50 bp single end sequencing/product/Illumina Inc
    Average 79 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    multiplexed 50 bp single end sequencing - by Bioz Stars, 2020-02
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    79
    Illumina Inc bp single end illumina gaii sequencing
    Barcode lentiviral vector, sequencing and analysis workflow. (a) The 20 bp DNA barcode was cloned into the non-coding region of a SIN (self-inactivating) lentiviral vector upstream of a UBC-eGFP cassette. The P5 <t>Illumina</t> sequencing adapter sequence was integrated next to the barcode, and the P7 adapter was added during the PCR amplification step (primer positions shown). (b) This PCR results in a 250 bp fragment that includes a 4 bp indexing tag to allow pooling of multiple samples into a single lane of a flow cell, in addition to the 20 bp random barcode sequence, and flanked on either side by eight 'anchor' bases, which act as markers to identify true barcode sequences within the sequencing data. Finally, the fragments contain a spacer of approximately 90 bp and the second (P7) Illumina adapter for sequencing. Integrating the adapter into the barcode vector allows for single-end 36 bp (short) sequencing reads in which the barcode end is always sequenced. (c) Data analysis workflow.
    Bp Single End Illumina Gaii Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp single end illumina gaii sequencing/product/Illumina Inc
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bp single end illumina gaii sequencing - by Bioz Stars, 2020-02
    79/100 stars
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    77
    Illumina Inc 101 bp single end illumina sequencing
    Barcode lentiviral vector, sequencing and analysis workflow. (a) The 20 bp DNA barcode was cloned into the non-coding region of a SIN (self-inactivating) lentiviral vector upstream of a UBC-eGFP cassette. The P5 <t>Illumina</t> sequencing adapter sequence was integrated next to the barcode, and the P7 adapter was added during the PCR amplification step (primer positions shown). (b) This PCR results in a 250 bp fragment that includes a 4 bp indexing tag to allow pooling of multiple samples into a single lane of a flow cell, in addition to the 20 bp random barcode sequence, and flanked on either side by eight 'anchor' bases, which act as markers to identify true barcode sequences within the sequencing data. Finally, the fragments contain a spacer of approximately 90 bp and the second (P7) Illumina adapter for sequencing. Integrating the adapter into the barcode vector allows for single-end 36 bp (short) sequencing reads in which the barcode end is always sequenced. (c) Data analysis workflow.
    101 Bp Single End Illumina Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/101 bp single end illumina sequencing/product/Illumina Inc
    Average 77 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    101 bp single end illumina sequencing - by Bioz Stars, 2020-02
    77/100 stars
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    76
    Illumina Inc 76 bp single end illumina sequencing
    Barcode lentiviral vector, sequencing and analysis workflow. (a) The 20 bp DNA barcode was cloned into the non-coding region of a SIN (self-inactivating) lentiviral vector upstream of a UBC-eGFP cassette. The P5 <t>Illumina</t> sequencing adapter sequence was integrated next to the barcode, and the P7 adapter was added during the PCR amplification step (primer positions shown). (b) This PCR results in a 250 bp fragment that includes a 4 bp indexing tag to allow pooling of multiple samples into a single lane of a flow cell, in addition to the 20 bp random barcode sequence, and flanked on either side by eight 'anchor' bases, which act as markers to identify true barcode sequences within the sequencing data. Finally, the fragments contain a spacer of approximately 90 bp and the second (P7) Illumina adapter for sequencing. Integrating the adapter into the barcode vector allows for single-end 36 bp (short) sequencing reads in which the barcode end is always sequenced. (c) Data analysis workflow.
    76 Bp Single End Illumina Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/76 bp single end illumina sequencing/product/Illumina Inc
    Average 76 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    76 bp single end illumina sequencing - by Bioz Stars, 2020-02
    76/100 stars
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    Illumina Inc bp single end cdna sequencing
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    Barcode lentiviral vector, sequencing and analysis workflow. (a) The 20 bp DNA barcode was cloned into the non-coding region of a SIN (self-inactivating) lentiviral vector upstream of a UBC-eGFP cassette. The P5 <t>Illumina</t> sequencing adapter sequence was integrated next to the barcode, and the P7 adapter was added during the PCR amplification step (primer positions shown). (b) This PCR results in a 250 bp fragment that includes a 4 bp indexing tag to allow pooling of multiple samples into a single lane of a flow cell, in addition to the 20 bp random barcode sequence, and flanked on either side by eight 'anchor' bases, which act as markers to identify true barcode sequences within the sequencing data. Finally, the fragments contain a spacer of approximately 90 bp and the second (P7) Illumina adapter for sequencing. Integrating the adapter into the barcode vector allows for single-end 36 bp (short) sequencing reads in which the barcode end is always sequenced. (c) Data analysis workflow.
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    Barcode lentiviral vector, sequencing and analysis workflow. (a) The 20 bp DNA barcode was cloned into the non-coding region of a SIN (self-inactivating) lentiviral vector upstream of a UBC-eGFP cassette. The P5 <t>Illumina</t> sequencing adapter sequence was integrated next to the barcode, and the P7 adapter was added during the PCR amplification step (primer positions shown). (b) This PCR results in a 250 bp fragment that includes a 4 bp indexing tag to allow pooling of multiple samples into a single lane of a flow cell, in addition to the 20 bp random barcode sequence, and flanked on either side by eight 'anchor' bases, which act as markers to identify true barcode sequences within the sequencing data. Finally, the fragments contain a spacer of approximately 90 bp and the second (P7) Illumina adapter for sequencing. Integrating the adapter into the barcode vector allows for single-end 36 bp (short) sequencing reads in which the barcode end is always sequenced. (c) Data analysis workflow.
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    Image Search Results


    A pooled screen for functional validation of CLD. a The screening strategy in SW480 cells. In brief, a pool of mutant SW480 cells harboring 12,471 sgRNA designs against 408 genes was generated by lentiviral infection and antibiotic selection. Fourteen million cells per condition were then treated with PBS (control) or recombinant TRAIL (treatment) for a total of 12 days. Subsequently, the genomic DNA of the samples was extracted and sgRNA composition analyzed by next-generation sequencing ( NGS ). b Comparison of sgRNA sequence counts between two biological replicates demonstrates high reproducibility (Pearson correlation coefficient ~0.79). c Distribution of sgRNAs targeting positive pathway regulators ( CASP8 , CASP3 , FADD , BAX , BID , TNFRSF10A , TNFRSF10B ) in red , negative regulators ( XIAP , BCL2L1 ) in blue , and random, non-targeting sgRNAs in orange between TRAIL ( y-axis ) and PBS ( x-axis ) treated cell populations. d Scatter plot showing relative enrichment of genes ( y-axis ) with their corresponding p value ( x-axis ). Positive regulators are plotted in red , negative regulators in blue , and random, non-targeting sgRNAs in orange. P values were calculated by Wilcoxon rank sum test between 30 sgRNAs of one gene and 200 random, non-targeting sgRNAs. Log 2 fold change is calculated as median log 2 ratio between normalized sgRNA count of TRAIL- over PBS-treated populations. The vertical line marks a p value of 0.05. e – g Median normalized fold change of all sgRNAs targeting three essential TRAIL pathway components. A total of 100 sgRNAs are depicted for each gene. Enriched sgRNAs are colored in red , depleted sgRNAs in grey . The dashed line represents the median fold change of all sgRNAs of the corresponding gene

    Journal: Genome Biology

    Article Title: CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries

    doi: 10.1186/s13059-016-0915-2

    Figure Lengend Snippet: A pooled screen for functional validation of CLD. a The screening strategy in SW480 cells. In brief, a pool of mutant SW480 cells harboring 12,471 sgRNA designs against 408 genes was generated by lentiviral infection and antibiotic selection. Fourteen million cells per condition were then treated with PBS (control) or recombinant TRAIL (treatment) for a total of 12 days. Subsequently, the genomic DNA of the samples was extracted and sgRNA composition analyzed by next-generation sequencing ( NGS ). b Comparison of sgRNA sequence counts between two biological replicates demonstrates high reproducibility (Pearson correlation coefficient ~0.79). c Distribution of sgRNAs targeting positive pathway regulators ( CASP8 , CASP3 , FADD , BAX , BID , TNFRSF10A , TNFRSF10B ) in red , negative regulators ( XIAP , BCL2L1 ) in blue , and random, non-targeting sgRNAs in orange between TRAIL ( y-axis ) and PBS ( x-axis ) treated cell populations. d Scatter plot showing relative enrichment of genes ( y-axis ) with their corresponding p value ( x-axis ). Positive regulators are plotted in red , negative regulators in blue , and random, non-targeting sgRNAs in orange. P values were calculated by Wilcoxon rank sum test between 30 sgRNAs of one gene and 200 random, non-targeting sgRNAs. Log 2 fold change is calculated as median log 2 ratio between normalized sgRNA count of TRAIL- over PBS-treated populations. The vertical line marks a p value of 0.05. e – g Median normalized fold change of all sgRNAs targeting three essential TRAIL pathway components. A total of 100 sgRNAs are depicted for each gene. Enriched sgRNAs are colored in red , depleted sgRNAs in grey . The dashed line represents the median fold change of all sgRNAs of the corresponding gene

    Article Snippet: The purified libraries were controlled for correct size using DNA High Sensitivity Assay on a BioAnalyzer 2100 (Agilent) and then sequenced on a MiSeq (Illumina) by 100-bp single-end sequencing and addition of 20 % PhiX Control v3 (Illumina) at a concentration of 8 pM.

    Techniques: Functional Assay, Mutagenesis, Generated, Infection, Selection, Recombinant, Next-Generation Sequencing, Sequencing

    Barcode lentiviral vector, sequencing and analysis workflow. (a) The 20 bp DNA barcode was cloned into the non-coding region of a SIN (self-inactivating) lentiviral vector upstream of a UBC-eGFP cassette. The P5 Illumina sequencing adapter sequence was integrated next to the barcode, and the P7 adapter was added during the PCR amplification step (primer positions shown). (b) This PCR results in a 250 bp fragment that includes a 4 bp indexing tag to allow pooling of multiple samples into a single lane of a flow cell, in addition to the 20 bp random barcode sequence, and flanked on either side by eight 'anchor' bases, which act as markers to identify true barcode sequences within the sequencing data. Finally, the fragments contain a spacer of approximately 90 bp and the second (P7) Illumina adapter for sequencing. Integrating the adapter into the barcode vector allows for single-end 36 bp (short) sequencing reads in which the barcode end is always sequenced. (c) Data analysis workflow.

    Journal: Genome Biology

    Article Title: Lentiviral and targeted cellular barcoding reveals ongoing clonal dynamics of cell lines in vitro and in vivo

    doi: 10.1186/gb-2014-15-5-r75

    Figure Lengend Snippet: Barcode lentiviral vector, sequencing and analysis workflow. (a) The 20 bp DNA barcode was cloned into the non-coding region of a SIN (self-inactivating) lentiviral vector upstream of a UBC-eGFP cassette. The P5 Illumina sequencing adapter sequence was integrated next to the barcode, and the P7 adapter was added during the PCR amplification step (primer positions shown). (b) This PCR results in a 250 bp fragment that includes a 4 bp indexing tag to allow pooling of multiple samples into a single lane of a flow cell, in addition to the 20 bp random barcode sequence, and flanked on either side by eight 'anchor' bases, which act as markers to identify true barcode sequences within the sequencing data. Finally, the fragments contain a spacer of approximately 90 bp and the second (P7) Illumina adapter for sequencing. Integrating the adapter into the barcode vector allows for single-end 36 bp (short) sequencing reads in which the barcode end is always sequenced. (c) Data analysis workflow.

    Article Snippet: The lentiviral vector was designed to include the Illumina P5 adapter sequence 8 bp upstream of the barcode sequence, facilitating amplification and sample preparation of the barcode sequences in a single PCR step, while positioning the barcode, allowing for the use of single-end 36 bp Illumina sequencing reads, and thus maximizing the barcode-to-cost ratio (Figure a).

    Techniques: Plasmid Preparation, Sequencing, Clone Assay, Polymerase Chain Reaction, Amplification, Flow Cytometry, Activated Clotting Time Assay