Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Trans-portal hepatic infusion of cultured bone marrow-derived mesenchymal stem cells in a steatohepatitis murine model

doi: 10.3164/jcbn.20-88

Figure Lengend Snippet: Quantification of liver fibrosis. Luciferase positive bone marrow-derived mesenchymal stem cells (Luc-BMSCs) adjusted to 1.0 × 10 6 cells/100 µl were infused into the spleen of a modified steatohepatitis murine model at 12 weeks of the choline-deficient, l -amino acid-defined diet (12W), and at 4 weeks after infusion, the animals were sacrificed ( n = 8). Liver fibrosis and liver steatosis were similarly induced in a control group that was infused with phosphate-buffered saline (PBS) into the spleen, and after 4 weeks, the animals were sacrificed ( n = 8). The evaluation of liver fibrosis is shown. (A) The mean level of Sirius red-stained area was significantly lower in the BMSC group. Control group vs BMSC group = 5.4 ± 0.9% vs 1.5 ± 0.2%. Error bars indicate SD, n = 8 mice each. ** p <0.01. Magnification ×40, scale bars = 100 µm. (B) Western blotting of α-smooth muscle actin (α-SMA) protein in the liver between the BMSC and control groups. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was used as an internal control, n = 3 each. (C) mRNA expression of collagen1a1 ( Col1a1 ) normalized with β-actin ( Actb ), n = 8 mice each. ** p <0.01.

Article Snippet: B6 mouse-derived BMSCs (passage 6) purchased from Cyagen Biosciences Inc. (Tokyo, Japan) were transduced with lentiviral vectors (#79692-G; BPS Bioscience, San Diego, CA) expressing luciferase, and passage 8 BMSCs (Luc-BMSCs) were used in the experimentation.

Techniques: Luciferase, Derivative Assay, Modification, Control, Saline, Staining, Western Blot, Expressing

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Trans-portal hepatic infusion of cultured bone marrow-derived mesenchymal stem cells in a steatohepatitis murine model

doi: 10.3164/jcbn.20-88

Figure Lengend Snippet: Quantification of liver steatosis and lipid metabolism in the liver. Luciferase positive bone marrow-derived mesenchymal stem cells (Luc-BMSCs) adjusted to 1.0 × 10 6 cells/100 µl were infused into the spleen of a modified steatohepatitis murine model at 12 weeks of the choline-deficient, l -amino acid-defined diet (12W), and, at 4 weeks after infusion, the animals were sacrificed ( n = 8). (A) Hematoxylin and eosin (HE) staining. The lipid droplet deposition area was significantly lower in the BMSC group (control group vs BMSC group = 34.7 ± 2.1% vs 21.2 ± 3.3%). Error bars indicate SD, n = 8 mice each. ** p <0.01. Magnifications ×40 and ×100. (B) mRNA expressions of genes related to lipid metabolism in the liver. n = 8 mice each. * p <0.05, ** p <0.01, ns, not significant. Error bars indicate SD.

Article Snippet: B6 mouse-derived BMSCs (passage 6) purchased from Cyagen Biosciences Inc. (Tokyo, Japan) were transduced with lentiviral vectors (#79692-G; BPS Bioscience, San Diego, CA) expressing luciferase, and passage 8 BMSCs (Luc-BMSCs) were used in the experimentation.

Techniques: Luciferase, Derivative Assay, Modification, Staining, Control

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Trans-portal hepatic infusion of cultured bone marrow-derived mesenchymal stem cells in a steatohepatitis murine model

doi: 10.3164/jcbn.20-88

Figure Lengend Snippet: Assessment of the effects of cultured bone marrow-derived mesenchymal stem cells (BMSCs) on steatosis in HepG2 cells. (A) Oil-red O staining of HepG2 cells (magnification ×40, ×100). Control group: HepG2 cells were exposed to 0.5 mM free fatty acid (FFA) mixture for 72 h in the 12-well plate. BMSC co-culture group: HepG2 cells were exposed to 0.5 mM FFA mixture for 24 h. Then, 5 × 10 4 BMSCs were seeded onto the 0.4-µm-pore size Cell Culture Insert and placed into the 12-well plate with the HepG2 cells, and these two kinds of cells were cultured for another 48 h. Scale bars = 100 µm. (B) Absorbance (492 nm) of Oil-red O staining solution. The mean absorbance at 492 nm of Oil-red O staining solution extracted from wells is shown ( n = 12 per group). Control group vs BMSC co-culture group = 1.4 ± 0.1 vs 1.2 ± 0.1. Error bars indicate SD, n = 12 each, ** p <0.01.

Article Snippet: B6 mouse-derived BMSCs (passage 6) purchased from Cyagen Biosciences Inc. (Tokyo, Japan) were transduced with lentiviral vectors (#79692-G; BPS Bioscience, San Diego, CA) expressing luciferase, and passage 8 BMSCs (Luc-BMSCs) were used in the experimentation.

Techniques: Cell Culture, Derivative Assay, Staining, Control, Co-Culture Assay, Pore Size

Journal: Stem Cells International

Article Title: Vascularization of a Bone Organoid Using Dental Pulp Stem Cells

doi: 10.1155/2023/5367887

Figure Lengend Snippet: Comparison of the sprouting ability of BMSCs and DPSCs. (a) Photomicrographs of sprouting capillary structures formed by BMSCs and DPSCs with endothelial induction. (b) Quantitative analyses of the number of branches and (c) total branching length at corresponding time points. Scale bars: 200 μ m. ∗ p < 0.05; mean ± SD; n = 4.

Article Snippet: Human BMSCs derived from iliac crests (Lonza, Basel, Switzerland) and DPSCs isolated from adult third molars (Lonza) were cultured in growth medium (GM) comprising Dulbecco's modified Eagle's medium (DMEM; Wako, Osaka, Japan) supplemented with 20% fetal bovine serum (Thermo Fisher Scientific, MA, USA) and 1% penicillin-streptomycin (Sigma-Aldrich, MO, USA).

Techniques: Comparison

Journal: Stem Cells International

Article Title: Vascularization of a Bone Organoid Using Dental Pulp Stem Cells

doi: 10.1155/2023/5367887

Figure Lengend Snippet: Comparison of endothelial differentiation ability of BMSCs and DPSCs. mRNA expression of (a) VEGFA , (b) CXCL1 , and (c) Nanog after 1, 3, 7, and 14 days of endothelial induction. Different capital letters indicate significant differences among groups ( p < 0.05); mean ± SD; n = 4.

Article Snippet: Human BMSCs derived from iliac crests (Lonza, Basel, Switzerland) and DPSCs isolated from adult third molars (Lonza) were cultured in growth medium (GM) comprising Dulbecco's modified Eagle's medium (DMEM; Wako, Osaka, Japan) supplemented with 20% fetal bovine serum (Thermo Fisher Scientific, MA, USA) and 1% penicillin-streptomycin (Sigma-Aldrich, MO, USA).

Techniques: Comparison, Expressing

Journal: Stem Cells International

Article Title: Vascularization of a Bone Organoid Using Dental Pulp Stem Cells

doi: 10.1155/2023/5367887

Figure Lengend Snippet: Structure of the cell constructs after endothelial and osteogenic induction. (a) HE staining, (b) von Kossa staining, and (c) fluorescence imaging of cell constructs with varying ratios of BMSCs and DPSCs. White arrows indicate a capillary-like hollow structure in the mineralized 80B/20D constructs. Dotted lines indicate the outline of the cell constructs. Scale bars: 50 μ m.

Article Snippet: Human BMSCs derived from iliac crests (Lonza, Basel, Switzerland) and DPSCs isolated from adult third molars (Lonza) were cultured in growth medium (GM) comprising Dulbecco's modified Eagle's medium (DMEM; Wako, Osaka, Japan) supplemented with 20% fetal bovine serum (Thermo Fisher Scientific, MA, USA) and 1% penicillin-streptomycin (Sigma-Aldrich, MO, USA).

Techniques: Construct, Staining, Fluorescence, Imaging