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Image Search Results
Journal: Journal of orthopaedic trauma
Article Title: The Effects of Episodic Alcohol Exposure on BMP2 Signaling During Tibia Fracture Healing
doi: 10.1097/BOT.0000000000001160
Figure Lengend Snippet: (A) Binge alcohol effects on Chordin protein expression in fracture callus. ELISA data of Chordin protein expression assessed at post fracture days 3, 7 14, and 21. Alcohol was shown to cause a significant increase in Chordin expression at all time points with the greatest difference occurring at post fracture day 14 (p<.001). (B-C) Effects of binge alcohol on SMAD4 and activated phospho Smad 1/5/9 protein expression in fracture callus. Representative western blot for SMAD4 and phospho Smad 1/5/9 protein levels in fracture callus tissue homogenate at post fracture day 14. Saline samples labeled (S) and alcohol samples labeled (A). To ensure the equal loading of protein, blot signal volumes were normalized against total protein concentrations. Western data was reported as a ratio of average signal volumes between the control and experimental groups. (B) Alcohol causes a significant increase in SMAD4 expression at post fracture day 14 as seen in the western blot data (p=.003). (C) Alcohol was shown to attenuate phospho Smad 1/5/9 expression at day 14, however this was not found to be significant (p=.08). Statistical significance was present when p <0.05 as determined by independent sample T-tests and displayed with (*).
Article Snippet: BMP Signaling Assays Protein expressions of BMP2, BMPRII, BMPR1A, and the inhibitor Chordin were all assessed via the following commercially available
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Saline, Labeling, Control