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Vector Biolabs
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Thermo Fisher
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R&D Systems
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OriGene
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Proteintech
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R&D Systems
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Santa Cruz Biotechnology
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OriGene
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OriGene
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Novus Biologicals
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Image Search Results
Journal: Journal of molecular biomarkers & diagnosis
Article Title: Methylated Bone Morphogenetic Protein 3 (BMP3) Gene: Evaluation of Tumor Suppressor Function and Biomarker Potential in Biliary Cancer
doi: 10.4172/2155-9929.1000145
Figure Lengend Snippet: (A) CpG island targeted in exon 1 of the BMP3 gene. (B) BMP3 is methylated by MSP in all five biliary cancer cell lines but not in the immortalized normal cholangiocyte cell line H-69.
Article Snippet: The sequence of the
Techniques: Methylation
Journal: Journal of molecular biomarkers & diagnosis
Article Title: Methylated Bone Morphogenetic Protein 3 (BMP3) Gene: Evaluation of Tumor Suppressor Function and Biomarker Potential in Biliary Cancer
doi: 10.4172/2155-9929.1000145
Figure Lengend Snippet: Bisulfite genomic sequencing methylation pattern of CpG sites for the targeted BMP3 island in ten clones from each of two malignant biliary cancer cell lines and the normal cholangiocyte cell line.
Article Snippet: The sequence of the
Techniques: Genomic Sequencing, Methylation, Clone Assay
Journal: Journal of molecular biomarkers & diagnosis
Article Title: Methylated Bone Morphogenetic Protein 3 (BMP3) Gene: Evaluation of Tumor Suppressor Function and Biomarker Potential in Biliary Cancer
doi: 10.4172/2155-9929.1000145
Figure Lengend Snippet: Natural logarithm transformed copy numbers of methylated BMP3 target sequences in cholangiocarcinoma (CC) tissue and paired benign bile duct epithelium samples from 12 patients
Article Snippet: The sequence of the
Techniques: Transformation Assay, Methylation
Journal: Journal of molecular biomarkers & diagnosis
Article Title: Methylated Bone Morphogenetic Protein 3 (BMP3) Gene: Evaluation of Tumor Suppressor Function and Biomarker Potential in Biliary Cancer
doi: 10.4172/2155-9929.1000145
Figure Lengend Snippet: Treatment with the DNA demethylating agent 5-Aza-2’-deoxycytidine (5-AZA) and the histone de-acetylase inhibitor trichostatin A (TSA) derepresses BMP3 gene expression in (A) KMC-1 cells and (B) Mz-ChA-1 cells.
Article Snippet: The sequence of the
Techniques: Gene Expression
Journal: Journal of molecular biomarkers & diagnosis
Article Title: Methylated Bone Morphogenetic Protein 3 (BMP3) Gene: Evaluation of Tumor Suppressor Function and Biomarker Potential in Biliary Cancer
doi: 10.4172/2155-9929.1000145
Figure Lengend Snippet: (A) BMP3 gene expression in Mz-ChA-1 cells 48 hours after transfection with BMP3 plasmid compared to control vector. (B) Forced expression of BMP3 inhibits Mz-ChA-1 cell proliferation as assessed by the BrdU assay and (C) reduces Mz-ChA-1 cell viability as measured by the MTT assay. (D) Transfection with BMP3 plasmid increased Mz-ChA-1 cell apoptosis at 48 hours, as measured by the relative fluorescence units in a caspase 3/7 assay.
Article Snippet: The sequence of the
Techniques: Gene Expression, Transfection, Plasmid Preparation, Control, Expressing, BrdU Staining, MTT Assay, Fluorescence