Journal: Nature Communications

Article Title: Self-splicing RNA circularization facilitated by intact group I and II introns

doi: 10.1038/s41467-025-62607-y

Figure Lengend Snippet: a Diagram illustrating payload types and sizes, with cited payloads from previous studies included. b Agarose gel electrophoresis showing spliced intron and RNA integrity of putative circRNA with varying payloads. c Qualitative validation of circRNA using RT-qPCR, since comparisons of the relative abundance of circRNA junctions across different payloads were not feasible. d − h Detection of protein expression for respective payloads: Gluc ( d ) and Fluc ( f ) detected with a microplate reader, EGFP ( e ) detected by FACS, and ABE8e ( g ) and dystrophin ( h ) detected by Western blot. Data are shown as mean ± S.D. ( n = 3), with each symbol representing a biological replicate. The linear RNA shown in this figure was produced using a modified group I intron with reduced activity. Source data are provided as a Source Data file.

Article Snippet: Primary antibodies specific to dystrophin (Abclonal, A1411, 1:250 dilution), vinculin (Abclonal, A2752, ARC51900 , 1:10,000 dilution), tubulin (Cwbio, CW0098M, 3G7, 1:5000 dilution), Cas9 (Beyotime, AF0123, 1:5000 dilution), and Flag (Easybio, BE2004, 1:10,000 dilution) were diluted in QuickBlockTM Primary Antibody Dilution Buffer for Western Blot (Beyotime, P0256).

Techniques: Agarose Gel Electrophoresis, Biomarker Discovery, Quantitative RT-PCR, Expressing, Western Blot, Produced, Modification, Activity Assay