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Image Search Results
Journal: Molecular Medicine Reports
Article Title: Inhibitory effects of oxymatrine on TGF-β1-induced proliferation and abnormal differentiation in rat cardiac fibroblasts via the p38MAPK and ERK1/2 signaling pathways
doi: 10.3892/mmr.2017.7277
Figure Lengend Snippet: Identification of CFBs (magnification, ×200). (A) Bright-field image of the 2nd passage CFBs. (B) Representative image of negative control stained cells (PBS was used instead of primary antibody). (C) Representative image of cells stained with the anti-vimentin antibody. CFBs, cardiac fibroblasts.
Article Snippet: The primary antibody,
Techniques: Negative Control, Staining
Journal: PLoS ONE
Article Title: Silencing SOX2 Induced Mesenchymal-Epithelial Transition and Its Expression Predicts Liver and Lymph Node Metastasis of CRC Patients
doi: 10.1371/journal.pone.0041335
Figure Lengend Snippet: (A) Western blot analysis of expression of E-cadherin and vimentin in SW620-mock and SW620-shRNA-SOX2 cells. GAPDH was used as a loading control. The right panel showed the right panel quantified by the Quantity One software (Bio-Rad). GAPDH was used for normalization. (B) Immunofluorescence images of SW620-mock and SW620-shRNA-SOX2 cells stained with antibodies against E-cadherin, vimentin and SOX2. Secondary antibody was conjugated with FITC and Cy3. Cell nuclei were stained with Hoechest 33258. (C) Semi-quantitative RT-PCR analysis of EMT-related regulatory factors. GAPDH was used as a loading control.
Article Snippet: Primary antibodies were diluted to incubate with PVDF membrane overnight at 4° [anti-SOX2 (2683-1
Techniques: Western Blot, Expressing, shRNA, Software, Immunofluorescence, Staining, Quantitative RT-PCR
Journal: Molecular Medicine Reports
Article Title: Overexpression of TG2 enhances the differentiation of ectomesenchymal stem cells into neuron-like cells and promotes functional recovery in adult rats following spinal cord injury
doi: 10.3892/mmr.2019.10502
Figure Lengend Snippet: Identification of EMSCs. (A) A representative image of primary EMSCs. Phase-contract images of EMSCs from passages (B) 3 and (C) 5. EMSCs of passage 3 positively expressed neural crest cell and mesenchymal stem cell markers, including (D) vimentin, (E) s100, (F) CD44 and (G) nestin. Scale bar, 50 µm. EMSCs, ectomesenchymal stem cells.
Article Snippet: EMSCs (passage 3) were identified by detecting the expression of nestin (rabbit polyclonal, ab27952, Abcam),
Techniques: