blp-pc056 Search Results


hcn1  (Alomone Labs)
90
Alomone Labs hcn1
Relative expression levels of <t>hcn1</t> , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.
Hcn1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hcn1 - by Bioz Stars, 2026-08
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Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.

Journal: International Journal of Molecular Sciences

Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

doi: 10.3390/ijms22147539

Figure Lengend Snippet: Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.

Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

Techniques: Expressing

Multiple clusters of pacemaker cells detected by the double immunolabeling with antibodies against Islet-1 (green signal), and HCN1 and HCN2 (red signal) in the SAR. ( a ) Pacemaker cells (arrows) embedded in the myocardium (M) expressing Islet-1 (green signal) and HCN1 (red signal). ( b – f ) Pacemaker cells revealed colocalization of Islet-1 and HCN2 ( d ; merge). Two populations expressing Islet-1 (green signal and arrows) and HCN2 (red signal and arrows), respectively, were observed in e and f. Scale bars: 20 µm.

Journal: International Journal of Molecular Sciences

Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

doi: 10.3390/ijms22147539

Figure Lengend Snippet: Multiple clusters of pacemaker cells detected by the double immunolabeling with antibodies against Islet-1 (green signal), and HCN1 and HCN2 (red signal) in the SAR. ( a ) Pacemaker cells (arrows) embedded in the myocardium (M) expressing Islet-1 (green signal) and HCN1 (red signal). ( b – f ) Pacemaker cells revealed colocalization of Islet-1 and HCN2 ( d ; merge). Two populations expressing Islet-1 (green signal and arrows) and HCN2 (red signal and arrows), respectively, were observed in e and f. Scale bars: 20 µm.

Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

Techniques: Immunolabeling, Expressing

Primary and secondary antibodies used in the present study.

Journal: International Journal of Molecular Sciences

Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

doi: 10.3390/ijms22147539

Figure Lengend Snippet: Primary and secondary antibodies used in the present study.

Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

Techniques:

Details of the primers used for relative quantification of mRNA levels by qPCR. In addition to the primer sequences used to quantify each transcript level, amplicon sizes, annealing temperatures (Ta) and amplification efficiencies (E) are indicated.

Journal: International Journal of Molecular Sciences

Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

doi: 10.3390/ijms22147539

Figure Lengend Snippet: Details of the primers used for relative quantification of mRNA levels by qPCR. In addition to the primer sequences used to quantify each transcript level, amplicon sizes, annealing temperatures (Ta) and amplification efficiencies (E) are indicated.

Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

Techniques: Quantitative Proteomics, Amplification, Sequencing