blocker b5 Search Results


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  • 99
    Thermo Fisher dntps
    Dntps, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 53087 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntps/product/Thermo Fisher
    Average 99 stars, based on 53087 article reviews
    Price from $9.99 to $1999.99
    dntps - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher platinium taq dna polymerase
    Platinium Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinium taq dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 186 article reviews
    Price from $9.99 to $1999.99
    platinium taq dna polymerase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher sybr green
    Sybr Green, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green/product/Thermo Fisher
    Average 99 stars, based on 29922 article reviews
    Price from $9.99 to $1999.99
    sybr green - by Bioz Stars, 2020-05
    99/100 stars
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    99
    Thermo Fisher pcr buffer
    (A) Melting curves of the downstream probe, LTprb, following <t>LATE-PCR</t> amplification for samples containing mixtures of wild type and mutant type targets. (B) Melting curves of the blocker, B5, as a probe following LATE-PCR amplification for 10,000 wild type targets or 1000, 100, 10 or 1 copy mutant type targets.
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28828 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/Thermo Fisher
    Average 99 stars, based on 28828 article reviews
    Price from $9.99 to $1999.99
    pcr buffer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    (A) Melting curves of the downstream probe, LTprb, following LATE-PCR amplification for samples containing mixtures of wild type and mutant type targets. (B) Melting curves of the blocker, B5, as a probe following LATE-PCR amplification for 10,000 wild type targets or 1000, 100, 10 or 1 copy mutant type targets.

    Journal: Scientific Reports

    Article Title: Kinetic Hairpin Oligonucleotide Blockers for Selective Amplification of Rare Mutations

    doi: 10.1038/srep05921

    Figure Lengend Snippet: (A) Melting curves of the downstream probe, LTprb, following LATE-PCR amplification for samples containing mixtures of wild type and mutant type targets. (B) Melting curves of the blocker, B5, as a probe following LATE-PCR amplification for 10,000 wild type targets or 1000, 100, 10 or 1 copy mutant type targets.

    Article Snippet: PCR Reaction mixtures LATE-PCR amplifications were performed using 25 μl reaction mixtures containing 1× PCR buffer (Invitrogen, Carlsbad, CA), 3 mM MgCl2, 200 nM dNTPs, 0.24× SYBR Green (Invitrogen, Carlsbad, CA), 50 nM limiting primer LP, 1000 nM excess primer XP/mmXP, 500 nM blocker B5/Off62, 100 nM molecular beacon probes LTprb/On68, 1 unit of Platinium Taq DNA polymerase (Invitrogen, Carlsbad, CA), and different concentrations of DNA target variants in the range of 1 to 10,000 copies, as determined by serial dilutions.

    Techniques: Polymerase Chain Reaction, Amplification, Mutagenesis

    Real-time SYBR fluorescence reading during LATE-PCR amplifications that include blocker B5 with different length of blocker binding step. (A) No blocker binding step;(B) 1-minute blocker binding step; (C) 2-minute blocker binding step.

    Journal: Scientific Reports

    Article Title: Kinetic Hairpin Oligonucleotide Blockers for Selective Amplification of Rare Mutations

    doi: 10.1038/srep05921

    Figure Lengend Snippet: Real-time SYBR fluorescence reading during LATE-PCR amplifications that include blocker B5 with different length of blocker binding step. (A) No blocker binding step;(B) 1-minute blocker binding step; (C) 2-minute blocker binding step.

    Article Snippet: PCR Reaction mixtures LATE-PCR amplifications were performed using 25 μl reaction mixtures containing 1× PCR buffer (Invitrogen, Carlsbad, CA), 3 mM MgCl2, 200 nM dNTPs, 0.24× SYBR Green (Invitrogen, Carlsbad, CA), 50 nM limiting primer LP, 1000 nM excess primer XP/mmXP, 500 nM blocker B5/Off62, 100 nM molecular beacon probes LTprb/On68, 1 unit of Platinium Taq DNA polymerase (Invitrogen, Carlsbad, CA), and different concentrations of DNA target variants in the range of 1 to 10,000 copies, as determined by serial dilutions.

    Techniques: Fluorescence, Polymerase Chain Reaction, Binding Assay