Journal: Molecular Medicine Reports

Article Title: Characterization of a bla NDM-1 -harboring plasmid from a Salmonella enterica clinical isolate in China

doi: 10.3892/mmr.2017.6733

Figure Lengend Snippet: Circular representation of pHS36-NDM. The innermost circle represents GC skew; the second inner circle represents GC content (blue, GC+; purple, GC-). The outermost double circle depicts gene distribution relative to repA (represented as an arrowhead) with colored boxes depicting functional category and direction of transcription; the outer band is the plus strand; inner band is the minus strand. The loci for a T4SS operon and class 1 integron are indicated in blue boxes. NDM, New Delhi metallo-β-lactamase-1; GC, guanine + cytosine; repA , replication protein A; T4SS, type IV secretion system; bla CMY6 , CMY-6 β-lactamase gene; sugE1 , quaternary ammonium compound resistance gene; dfrA12 , trimethoprim resistance gene; aadA2 , aminoglycoside resistance gene; qacEΔ1 , truncated quaternary ammonium compound resistance gene; sul1 , sulfonamide resistance gene; rmtC , rRNA methyltransferase; bla NDM-1 , New Delhi metallo-β-lactamase-1; ble MBL , bleomycin resistance gene.

Article Snippet: Two multidrug resistance gene islands were identified: The IS Ecp1-bla CMY transposition unit which contains a CMY-6 β-lactamase gene ( bla CMY-6 ) and a quaternary ammonium compound resistance gene ( sugE ); and the intI1 -IS CR27 accessory region, which contained a trimethoprim resistance gene ( dfrA12 ), two aminoglycoside resistance genes ( aadA2 and rmtC ), a truncated quaternary ammonium compound resistance gene ( qacE∆1 ), a sulfonamide resistance gene ( sul1 ), the bla NDM-1 carbapenemase and a bleomycin resistance gene ( ble MBL ). pHS36-NDM shared high homology with other bla NDM-1 -containing plasmids reported in Sweden, Italy and Japan.

Techniques: Functional Assay