Journal: Frontiers in Microbiology
Article Title: A Bacterial Surface Display System Expressing Cleavable Capsid Proteins of Human Norovirus: A Novel System to Discover Candidate Receptors
Figure Lengend Snippet: SDS-PAGE (A) and Western Blot (B) analysis of the expression of E. coli cell surface display systems for HuNoV (GII.4) recombinant capsid proteins with (3) or without the linker fragment (2) and the thrombin-released P proteins (4). M: prestained protein ladder (Catalog No.: 26616, Thermo Fisher, Shanghai, China); (1) pET-28a/BL21; (2) pET28a-inaQn-P (GII.4)/BL21; (3) pET28a-inaQn-TB-P (GII.4) /BL21; (4) Thrombin-released P proteins.
Article Snippet: Bacterial Strains and Construction of the Recombinant Plasmids Competent E. coli DH5α and BL21 (Thermo Fisher, Shanghai, China) were used for recombinant plasmid construction and protein expression, respectively.
Techniques: SDS Page, Western Blot, Expressing, Recombinant, Positron Emission Tomography