bl21 Thermo Fisher Search Results


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  • 98
    Thermo Fisher e coli bl21 ai thermo fisher
    SDS-PAGE (A) and Western Blot (B) analysis of the expression of E. coli cell surface display systems for HuNoV (GII.4) recombinant capsid proteins with (3) or without the linker fragment (2) and the thrombin-released P proteins (4). M: prestained protein ladder (Catalog No.: 26616, Thermo Fisher, Shanghai, China); (1) <t>pET-28a/BL21;</t> (2) pET28a-inaQn-P (GII.4)/BL21; (3) pET28a-inaQn-TB-P (GII.4) /BL21; (4) Thrombin-released P proteins.
    E Coli Bl21 Ai Thermo Fisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 604 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 604 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 ai thermo fisher - by Bioz Stars, 2020-02
    98/100 stars
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    99
    Thermo Fisher bl21 star
    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and <t>BL21</t> Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).
    Bl21 Star, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 759 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 759 article reviews
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    bl21 star - by Bioz Stars, 2020-02
    99/100 stars
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    99
    Thermo Fisher bl21 star de3
    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and <t>BL21</t> Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).
    Bl21 Star De3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 363 article reviews
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    bl21 star de3 - by Bioz Stars, 2020-02
    99/100 stars
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    84
    Thermo Fisher bl21 de3 plys
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 De3 Plys, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 40 article reviews
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    97
    Thermo Fisher bl21 plyss
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Plyss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 36 article reviews
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    99
    Thermo Fisher coli bl21 al
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Coli Bl21 Al, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 22 article reviews
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    80
    Thermo Fisher bl21 si
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Si, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 15 article reviews
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    80/100 stars
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    91
    Thermo Fisher bl21 de3 rosetta
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 De3 Rosetta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 13 article reviews
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    94
    Thermo Fisher bl21 gold
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Gold, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 19 article reviews
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    bl21 gold - by Bioz Stars, 2020-02
    94/100 stars
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    99
    Thermo Fisher prsetb
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Prsetb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 878 article reviews
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    99
    Thermo Fisher bl21 de3 competent cells
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 De3 Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 70 article reviews
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    bl21 de3 competent cells - by Bioz Stars, 2020-02
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    99
    Thermo Fisher bl21 codonplus
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Codonplus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 23 article reviews
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    bl21 codonplus - by Bioz Stars, 2020-02
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    81
    Thermo Fisher bl21 ai bacteria
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Ai Bacteria, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 81 stars, based on 16 article reviews
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    99
    Thermo Fisher bl21 startm de3
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Startm De3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 48 article reviews
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    bl21 startm de3 - by Bioz Stars, 2020-02
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    99
    Thermo Fisher bl21 star de3 competent cells
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Star De3 Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 130 article reviews
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    bl21 star de3 competent cells - by Bioz Stars, 2020-02
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    98
    Thermo Fisher bl21 de3 gold cells
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 De3 Gold Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 6 article reviews
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    80
    Thermo Fisher bl21 star bacteria
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Star Bacteria, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 13 article reviews
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    78
    Thermo Fisher bl21 codon bl21 ripl
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Codon Bl21 Ripl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78/100 stars
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    78
    Thermo Fisher oneshot bl21 cells
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Oneshot Bl21 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 2 article reviews
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    oneshot bl21 cells - by Bioz Stars, 2020-02
    78/100 stars
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    89
    Thermo Fisher bl21 star de3 plyss
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Star De3 Plyss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 21 article reviews
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    89/100 stars
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    78
    Thermo Fisher bl21 star de3 prare
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Star De3 Prare, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 2 article reviews
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    78/100 stars
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    79
    Thermo Fisher plyse bl21 de3 competent cells
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Plyse Bl21 De3 Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 8 article reviews
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    83
    Thermo Fisher bl21 bacterial cells
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Bacterial Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher bl21 de3 condon ril
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 De3 Condon Ril, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher bl21 plyss bacteria
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Plyss Bacteria, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher competent bl21 gold de3
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Competent Bl21 Gold De3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78/100 stars
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    79
    Thermo Fisher bl21 a1 bacteria
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 A1 Bacteria, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher bl21 de3 bacterial strains
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 De3 Bacterial Strains, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 5 article reviews
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    78/100 stars
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    92
    Thermo Fisher deep frozen bl21 star de3 cells
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Deep Frozen Bl21 Star De3 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bl21 competent cells
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bl21 star de3 bacterial cells
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 Star De3 Bacterial Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bl21 rosetta
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
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    Image Search Results


    SDS-PAGE (A) and Western Blot (B) analysis of the expression of E. coli cell surface display systems for HuNoV (GII.4) recombinant capsid proteins with (3) or without the linker fragment (2) and the thrombin-released P proteins (4). M: prestained protein ladder (Catalog No.: 26616, Thermo Fisher, Shanghai, China); (1) pET-28a/BL21; (2) pET28a-inaQn-P (GII.4)/BL21; (3) pET28a-inaQn-TB-P (GII.4) /BL21; (4) Thrombin-released P proteins.

    Journal: Frontiers in Microbiology

    Article Title: A Bacterial Surface Display System Expressing Cleavable Capsid Proteins of Human Norovirus: A Novel System to Discover Candidate Receptors

    doi: 10.3389/fmicb.2017.02405

    Figure Lengend Snippet: SDS-PAGE (A) and Western Blot (B) analysis of the expression of E. coli cell surface display systems for HuNoV (GII.4) recombinant capsid proteins with (3) or without the linker fragment (2) and the thrombin-released P proteins (4). M: prestained protein ladder (Catalog No.: 26616, Thermo Fisher, Shanghai, China); (1) pET-28a/BL21; (2) pET28a-inaQn-P (GII.4)/BL21; (3) pET28a-inaQn-TB-P (GII.4) /BL21; (4) Thrombin-released P proteins.

    Article Snippet: Bacterial Strains and Construction of the Recombinant Plasmids Competent E. coli DH5α and BL21 (Thermo Fisher, Shanghai, China) were used for recombinant plasmid construction and protein expression, respectively.

    Techniques: SDS Page, Western Blot, Expressing, Recombinant, Positron Emission Tomography

    OD of type A HBGA bound to serially diluted thrombin-released P proteins (red) and the thrombin-released background proteins from pET28a-inaQn-TB/BL21 (black). Bars represent standard errors.

    Journal: Frontiers in Microbiology

    Article Title: A Bacterial Surface Display System Expressing Cleavable Capsid Proteins of Human Norovirus: A Novel System to Discover Candidate Receptors

    doi: 10.3389/fmicb.2017.02405

    Figure Lengend Snippet: OD of type A HBGA bound to serially diluted thrombin-released P proteins (red) and the thrombin-released background proteins from pET28a-inaQn-TB/BL21 (black). Bars represent standard errors.

    Article Snippet: Bacterial Strains and Construction of the Recombinant Plasmids Competent E. coli DH5α and BL21 (Thermo Fisher, Shanghai, China) were used for recombinant plasmid construction and protein expression, respectively.

    Techniques:

    Physiological effects of rate of membrane protein production. CX21-GFP was produced by rapid, restrained, and basal expression in BL21-AI cells. A: Biomass production. Growth was monitored via OD 600 nm of culture. B: Cell viability analysis using the

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions

    doi: 10.1002/pro.535

    Figure Lengend Snippet: Physiological effects of rate of membrane protein production. CX21-GFP was produced by rapid, restrained, and basal expression in BL21-AI cells. A: Biomass production. Growth was monitored via OD 600 nm of culture. B: Cell viability analysis using the

    Article Snippet: The following E. coli strains were used to express proteins from T7 lac promoter based vectors: BL21(DE3) and BL21(DE3) pLysS (Novagen, CA), C43(DE3) (Lucigen, WI), Lemo21(DE3) (NEB, MA), and BL21-AI (Invitrogen, CA).

    Techniques: Produced, Expressing

    Validation of the restrained-expression method to produce toxic proteins. 6K protein, an ion channel from Sindibis virus, was expressed as a GFP fusion in BL21-AI and BL21(DE3) cells. Cell growth (A) was monitored at 0, 2, 6, and 20 h postinduction by

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions

    doi: 10.1002/pro.535

    Figure Lengend Snippet: Validation of the restrained-expression method to produce toxic proteins. 6K protein, an ion channel from Sindibis virus, was expressed as a GFP fusion in BL21-AI and BL21(DE3) cells. Cell growth (A) was monitored at 0, 2, 6, and 20 h postinduction by

    Article Snippet: The following E. coli strains were used to express proteins from T7 lac promoter based vectors: BL21(DE3) and BL21(DE3) pLysS (Novagen, CA), C43(DE3) (Lucigen, WI), Lemo21(DE3) (NEB, MA), and BL21-AI (Invitrogen, CA).

    Techniques: Expressing

    Comparative analysis of overexpression methods. A: Application of the restrained-expression method to improve expression of CX homologs. Fluorescence of GFP fused to CX homologs expressed in BL21-AI and BL21(DE3) cells induced with 0.01% (w/v) arabinose

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions

    doi: 10.1002/pro.535

    Figure Lengend Snippet: Comparative analysis of overexpression methods. A: Application of the restrained-expression method to improve expression of CX homologs. Fluorescence of GFP fused to CX homologs expressed in BL21-AI and BL21(DE3) cells induced with 0.01% (w/v) arabinose

    Article Snippet: The following E. coli strains were used to express proteins from T7 lac promoter based vectors: BL21(DE3) and BL21(DE3) pLysS (Novagen, CA), C43(DE3) (Lucigen, WI), Lemo21(DE3) (NEB, MA), and BL21-AI (Invitrogen, CA).

    Techniques: Over Expression, Expressing, Fluorescence

    Improving membrane protein expression. A and B: Tuning target gene expression in BL21-AI cells. Fluorescence (AFU/mL) of cultures expressing either GFP (A) or CX21-GFP (B) using restrained and rapid expression measured 20 h after addition of inducer(s)

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions

    doi: 10.1002/pro.535

    Figure Lengend Snippet: Improving membrane protein expression. A and B: Tuning target gene expression in BL21-AI cells. Fluorescence (AFU/mL) of cultures expressing either GFP (A) or CX21-GFP (B) using restrained and rapid expression measured 20 h after addition of inducer(s)

    Article Snippet: The following E. coli strains were used to express proteins from T7 lac promoter based vectors: BL21(DE3) and BL21(DE3) pLysS (Novagen, CA), C43(DE3) (Lucigen, WI), Lemo21(DE3) (NEB, MA), and BL21-AI (Invitrogen, CA).

    Techniques: Expressing, Fluorescence

    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and BL21 Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).

    Journal: PLoS ONE

    Article Title: An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli

    doi: 10.1371/journal.pone.0076913

    Figure Lengend Snippet: Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and BL21 Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).

    Article Snippet: Over-production of archaeal membrane transport proteins Expression constructs were used to transform two E. coli strains, BL21 Star (Invitrogen) and C43(DE3) [ ], containing the pRARE2 plasmid.

    Techniques: Expressing

    Affinity purification of seven membrane transport proteins. ( A ) Protein purification by Ni-NTA chromatography of 13 constructs expressed in E. coli BL21 Star. SDS-PAGE (top panel) and Western blot (bottom panel) of purified protein. MW - Bio-rad precision plus protein standards. ( B ) Estimated protein yield per litre of cell culture after initial Ni-NTA affinity chromatography. N/D - not determined.

    Journal: PLoS ONE

    Article Title: An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli

    doi: 10.1371/journal.pone.0076913

    Figure Lengend Snippet: Affinity purification of seven membrane transport proteins. ( A ) Protein purification by Ni-NTA chromatography of 13 constructs expressed in E. coli BL21 Star. SDS-PAGE (top panel) and Western blot (bottom panel) of purified protein. MW - Bio-rad precision plus protein standards. ( B ) Estimated protein yield per litre of cell culture after initial Ni-NTA affinity chromatography. N/D - not determined.

    Article Snippet: Over-production of archaeal membrane transport proteins Expression constructs were used to transform two E. coli strains, BL21 Star (Invitrogen) and C43(DE3) [ ], containing the pRARE2 plasmid.

    Techniques: Affinity Purification, Protein Purification, Chromatography, Construct, SDS Page, Western Blot, Purification, Cell Culture, Affinity Chromatography

    Western blot analysis of archaeal membrane transport proteins in E. coli membranes. Membrane transport protein genes cloned into pTTQ18, pET52b and pWarf were tested in two E. coli host strains, C43(DE3) and BL21 Star. Membranes were analysed Western blotting. C - C43(DE3), S - BL21 Star, 1 - pTTQ18, 2 - pET52b(+), 3 - pWarf(-).

    Journal: PLoS ONE

    Article Title: An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli

    doi: 10.1371/journal.pone.0076913

    Figure Lengend Snippet: Western blot analysis of archaeal membrane transport proteins in E. coli membranes. Membrane transport protein genes cloned into pTTQ18, pET52b and pWarf were tested in two E. coli host strains, C43(DE3) and BL21 Star. Membranes were analysed Western blotting. C - C43(DE3), S - BL21 Star, 1 - pTTQ18, 2 - pET52b(+), 3 - pWarf(-).

    Article Snippet: Over-production of archaeal membrane transport proteins Expression constructs were used to transform two E. coli strains, BL21 Star (Invitrogen) and C43(DE3) [ ], containing the pRARE2 plasmid.

    Techniques: Western Blot, Clone Assay

    SDS-PAGE analysis of archaeal membrane transport proteins in E. coli membranes. Membrane transport protein genes cloned into pTTQ18, pET52b and pWarf were tested in two E. coli host strains, C43(DE3) and BL21 Star. Membranes were analysed by SDS-PAGE. C - C43(DE3), S - BL21 Star, 1 - pTTQ18, 2 - pET52b(+), 3 - pWarf(-). Positions of the His-tagged protein determined by Western blotting (see Figure 3 ) are indicated by: triangle - protein detected by Western blotting but not visible on SDS-PAGE; star - protein detected by Western blotting and visible on SDS-PAGE gels.

    Journal: PLoS ONE

    Article Title: An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli

    doi: 10.1371/journal.pone.0076913

    Figure Lengend Snippet: SDS-PAGE analysis of archaeal membrane transport proteins in E. coli membranes. Membrane transport protein genes cloned into pTTQ18, pET52b and pWarf were tested in two E. coli host strains, C43(DE3) and BL21 Star. Membranes were analysed by SDS-PAGE. C - C43(DE3), S - BL21 Star, 1 - pTTQ18, 2 - pET52b(+), 3 - pWarf(-). Positions of the His-tagged protein determined by Western blotting (see Figure 3 ) are indicated by: triangle - protein detected by Western blotting but not visible on SDS-PAGE; star - protein detected by Western blotting and visible on SDS-PAGE gels.

    Article Snippet: Over-production of archaeal membrane transport proteins Expression constructs were used to transform two E. coli strains, BL21 Star (Invitrogen) and C43(DE3) [ ], containing the pRARE2 plasmid.

    Techniques: SDS Page, Clone Assay, Western Blot

    Expression and purification of fusion protein. 1—pET16b–scFv-DAF/BL21 (DE3) plyss before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.

    Journal: Muscle & nerve

    Article Title: PROTECTIVE EFFECT OF ScFv-DAF FUSION PROTEIN ON THE COMPLEMENT ATTACK TO ACETYLCHOLINE RECEPTOR: A POSSIBLE OPTION FOR TREATMENT OF MYASTHENIA GRAVIS

    doi: 10.1002/mus.23247

    Figure Lengend Snippet: Expression and purification of fusion protein. 1—pET16b–scFv-DAF/BL21 (DE3) plyss before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.

    Article Snippet: BL21 (DE3) pLyss (Invitrogen) bacteria transformed with the reconstructed plasmid pET16b were grown at 37°C in 2× YT medium containing 100 μ g/ml ampicillin and 50 μ g/ml chloramphenicol.

    Techniques: Expressing, Purification