bl21 Search Results


mesophilic host escherichia coli bl21  (ATCC)
96
ATCC mesophilic host escherichia coli bl21
Mesophilic Host Escherichia Coli Bl21, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bl21 de3 competent e coli  (New England Biolabs)
99
New England Biolabs bl21 de3 competent e coli
Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in <t>E.</t> <t>coli</t> <t>BL21(DE3),</t> and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
Bl21 De3 Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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virus strains e coli strain bl21 de3 sangon biotech b528419 e coli strain dh5α de3  (Sangon Biotech)
86
Sangon Biotech virus strains e coli strain bl21 de3 sangon biotech b528419 e coli strain dh5α de3
Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in <t>E.</t> <t>coli</t> <t>BL21(DE3),</t> and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
Virus Strains E Coli Strain Bl21 De3 Sangon Biotech B528419 E Coli Strain Dh5α De3, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli bl21 de3 cells  (Danaher Inc)
96
Danaher Inc e coli bl21 de3 cells
Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in <t>E.</t> <t>coli</t> <t>BL21(DE3),</t> and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
E Coli Bl21 De3 Cells, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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virus strains escherichia coli bl21 de3 tiangen biotech cb105 02 escherichia coli rosetta  (tiangen biotech co)
99
tiangen biotech co virus strains escherichia coli bl21 de3 tiangen biotech cb105 02 escherichia coli rosetta
Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in <t>E.</t> <t>coli</t> <t>BL21(DE3),</t> and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
Virus Strains Escherichia Coli Bl21 De3 Tiangen Biotech Cb105 02 Escherichia Coli Rosetta, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli bl21 de3 plyss cells  (tiangen biotech co)
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tiangen biotech co e coli bl21 de3 plyss cells
Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in <t>E.</t> <t>coli</t> <t>BL21(DE3),</t> and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
E Coli Bl21 De3 Plyss Cells, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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his tage fusion protein in bl21 de3 e coli  (tiangen biotech co)
99
tiangen biotech co his tage fusion protein in bl21 de3 e coli
Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in <t>E.</t> <t>coli</t> <t>BL21(DE3),</t> and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
His Tage Fusion Protein In Bl21 De3 E Coli, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cb101 bl21 de3 competent cells tiangen  (tiangen biotech co)
99
tiangen biotech co cb101 bl21 de3 competent cells tiangen
Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in <t>E.</t> <t>coli</t> <t>BL21(DE3),</t> and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
Cb101 Bl21 De3 Competent Cells Tiangen, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli bl21  (Cytiva Europe)
96
Cytiva Europe e coli bl21
Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in <t>E.</t> <t>coli</t> <t>BL21(DE3),</t> and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
E Coli Bl21, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli strain bl21 de3 δserb  (Addgene inc)
92
Addgene inc escherichia coli strain bl21 de3 δserb
Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in <t>E.</t> <t>coli</t> <t>BL21(DE3),</t> and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
Escherichia Coli Strain Bl21 De3 δserb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli bl21  (New England Biolabs)
96
New England Biolabs e coli bl21
Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in <t>E.</t> <t>coli</t> <t>BL21(DE3),</t> and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
E Coli Bl21, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli bl21 de3  (ATCC)
94
ATCC escherichia coli bl21 de3
Figure 1. Phylogenetic tree of Bacillus spp. based on 16S rRNA sequences through maximum-likelihood methods with 1000 bootstrap replications using MEGA version 6.0. <t>Escherichia</t> <t>coli</t> <t>BL21(DE3)</t> <t>(AM946981.2)</t> was used as an outgroup in the analysis. NCBI GenBank accession numbers used in the comparison reference strains are the following: B. velezensis FZB42, OR485707; B. amyloliquefaciens ATCC 23350, NR_118950; B. licheniformis ATCC 14580, NC_006270.3; B. aerius MN-1, MN252912; B. safensis ATCC BAA-1126T, AF234854; B. aryabhattai B8W22, NZ_JYOO01000023. NCBI acc. no. of the newly isolated strains are OR482395, OR482394, OR482396, OR482397, OP554433, MK461937, MK461933, MK461938, MK461934, MK461936, OR482398, OR482400, OR482399, MK461947.
Escherichia Coli Bl21 De3, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in E. coli BL21(DE3), and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.

Journal: Microorganisms

Article Title: Identification of Phage RNA Polymerases That Minimize Double-Stranded RNA By-Product Formation and Their Characterization via In Vitro Transcription

doi: 10.3390/microorganisms14030564

Figure Lengend Snippet: Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in E. coli BL21(DE3), and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.

Article Snippet: BL21(DE3) Competent E. coli , C2527H , NEB Inc..

Techniques: Expressing, SDS Page, Plasmid Preparation, Control, Derivative Assay, SDS-Gel

Figure 1. Phylogenetic tree of Bacillus spp. based on 16S rRNA sequences through maximum-likelihood methods with 1000 bootstrap replications using MEGA version 6.0. Escherichia coli BL21(DE3) (AM946981.2) was used as an outgroup in the analysis. NCBI GenBank accession numbers used in the comparison reference strains are the following: B. velezensis FZB42, OR485707; B. amyloliquefaciens ATCC 23350, NR_118950; B. licheniformis ATCC 14580, NC_006270.3; B. aerius MN-1, MN252912; B. safensis ATCC BAA-1126T, AF234854; B. aryabhattai B8W22, NZ_JYOO01000023. NCBI acc. no. of the newly isolated strains are OR482395, OR482394, OR482396, OR482397, OP554433, MK461937, MK461933, MK461938, MK461934, MK461936, OR482398, OR482400, OR482399, MK461947.

Journal: Biotechnology & Biotechnological Equipment

Article Title: Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues

doi: 10.1080/13102818.2024.2313072

Figure Lengend Snippet: Figure 1. Phylogenetic tree of Bacillus spp. based on 16S rRNA sequences through maximum-likelihood methods with 1000 bootstrap replications using MEGA version 6.0. Escherichia coli BL21(DE3) (AM946981.2) was used as an outgroup in the analysis. NCBI GenBank accession numbers used in the comparison reference strains are the following: B. velezensis FZB42, OR485707; B. amyloliquefaciens ATCC 23350, NR_118950; B. licheniformis ATCC 14580, NC_006270.3; B. aerius MN-1, MN252912; B. safensis ATCC BAA-1126T, AF234854; B. aryabhattai B8W22, NZ_JYOO01000023. NCBI acc. no. of the newly isolated strains are OR482395, OR482394, OR482396, OR482397, OP554433, MK461937, MK461933, MK461938, MK461934, MK461936, OR482398, OR482400, OR482399, MK461947.

Article Snippet: Escherichia coli Bl21(De3) (am946981.2) was used as an outgroup in the analysis. ncBi genBank accession numbers used in the comparison reference strains are the following: B. velezensis FZB42, oR485707; B. amyloliquefaciens atcc 23350, nR_118950; B. licheniformis atcc 14580, nc_006270.3; B. aerius mn-1, mn252912; B. safensis atcc Baa-1126t, aF234854; B. aryabhattai B8W22, nZ_Jyoo01000023. ncBi acc. no. of the newly isolated strains are oR482395, oR482394, oR482396, oR482397, op554433, mK461937, mK461933, mK461938, mK461934, mK461936, oR482398, oR482400, oR482399, mK461947.

Techniques: Comparison, Isolation

Figure 3. PCR amplification of fenA gene encoding fengycin synthetase A. Designations: M, molecular weight marker (Perfect PlusTM 1 kb DNA Ladder, EURx); 1, B. velezensis R7; 2, B. amyloliquefaciens R10; 3, B. velezensis R19; 4, B. velezensis R22; 5, B. velezensis R23; 6, negative control (E. coli DH5α).

Journal: Biotechnology & Biotechnological Equipment

Article Title: Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues

doi: 10.1080/13102818.2024.2313072

Figure Lengend Snippet: Figure 3. PCR amplification of fenA gene encoding fengycin synthetase A. Designations: M, molecular weight marker (Perfect PlusTM 1 kb DNA Ladder, EURx); 1, B. velezensis R7; 2, B. amyloliquefaciens R10; 3, B. velezensis R19; 4, B. velezensis R22; 5, B. velezensis R23; 6, negative control (E. coli DH5α).

Article Snippet: Escherichia coli Bl21(De3) (am946981.2) was used as an outgroup in the analysis. ncBi genBank accession numbers used in the comparison reference strains are the following: B. velezensis FZB42, oR485707; B. amyloliquefaciens atcc 23350, nR_118950; B. licheniformis atcc 14580, nc_006270.3; B. aerius mn-1, mn252912; B. safensis atcc Baa-1126t, aF234854; B. aryabhattai B8W22, nZ_Jyoo01000023. ncBi acc. no. of the newly isolated strains are oR482395, oR482394, oR482396, oR482397, op554433, mK461937, mK461933, mK461938, mK461934, mK461936, oR482398, oR482400, oR482399, mK461947.

Techniques: Amplification, Molecular Weight, Marker, Negative Control

Figure 4. PCR amplification of specific fragments of srfAA gene encoding surfactin synthetase A (A) and lchAA gene for lichenysin synthase (B). Lanes and samples: M, molecular weight marker (Perfect PlusTM 1 kb DNA Ladder, EURx); (A): 1, B. velezensis R7; 2, B. amyloliquefaciens R10; 3, B. velezensis R19; 4, B. velezensis R22; 5, B. velezensis R23; 6, negative control (E. coli DH5α); (B) 1, B. licheniformis 16-1; 2, B. licheniformis 13; 3, B. licheniformis 24; 4, B. licheniformis 39; 5, B. licheniformis 55-1; 6, negative control (E. coli DH5α).

Journal: Biotechnology & Biotechnological Equipment

Article Title: Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues

doi: 10.1080/13102818.2024.2313072

Figure Lengend Snippet: Figure 4. PCR amplification of specific fragments of srfAA gene encoding surfactin synthetase A (A) and lchAA gene for lichenysin synthase (B). Lanes and samples: M, molecular weight marker (Perfect PlusTM 1 kb DNA Ladder, EURx); (A): 1, B. velezensis R7; 2, B. amyloliquefaciens R10; 3, B. velezensis R19; 4, B. velezensis R22; 5, B. velezensis R23; 6, negative control (E. coli DH5α); (B) 1, B. licheniformis 16-1; 2, B. licheniformis 13; 3, B. licheniformis 24; 4, B. licheniformis 39; 5, B. licheniformis 55-1; 6, negative control (E. coli DH5α).

Article Snippet: Escherichia coli Bl21(De3) (am946981.2) was used as an outgroup in the analysis. ncBi genBank accession numbers used in the comparison reference strains are the following: B. velezensis FZB42, oR485707; B. amyloliquefaciens atcc 23350, nR_118950; B. licheniformis atcc 14580, nc_006270.3; B. aerius mn-1, mn252912; B. safensis atcc Baa-1126t, aF234854; B. aryabhattai B8W22, nZ_Jyoo01000023. ncBi acc. no. of the newly isolated strains are oR482395, oR482394, oR482396, oR482397, op554433, mK461937, mK461933, mK461938, mK461934, mK461936, oR482398, oR482400, oR482399, mK461947.

Techniques: Amplification, Molecular Weight, Marker, Negative Control