bkα Search Results


bkα subunit  (Addgene inc)
93
Addgene inc bkα subunit
Representative blot and densitometric analysis showing the co-precipitation of <t>BKα</t> <t>with</t> <t>BKβ1</t> from HEK293 cells overexpressing either one or both BK subunits and stimulated with 11,12-EET (10 µmol/L) for 2–10 minutes. The graph summarizes data from 6 independent experiments; *P<0.05 versus the unstimulated control (CTL).
Bkα Subunit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/pmc03454360-39-13-17?v=Addgene+inc
Average 93 stars, based on 1 article reviews
bkα subunit - by Bioz Stars, 2026-08
93/100 stars
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mouse anti slo1 bkalpha potassium channel antibody  (NeuroMab)
93
NeuroMab mouse anti slo1 bkalpha potassium channel antibody
Representative blot and densitometric analysis showing the co-precipitation of <t>BKα</t> <t>with</t> <t>BKβ1</t> from HEK293 cells overexpressing either one or both BK subunits and stimulated with 11,12-EET (10 µmol/L) for 2–10 minutes. The graph summarizes data from 6 independent experiments; *P<0.05 versus the unstimulated control (CTL).
Mouse Anti Slo1 Bkalpha Potassium Channel Antibody, supplied by NeuroMab, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/pmc10369482-196-16-23?v=NeuroMab
Average 93 stars, based on 1 article reviews
mouse anti slo1 bkalpha potassium channel antibody - by Bioz Stars, 2026-08
93/100 stars
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the fap-bkα construct  (GenScript corporation)
90
GenScript corporation the fap-bkα construct
Representative blot and densitometric analysis showing the co-precipitation of <t>BKα</t> <t>with</t> <t>BKβ1</t> from HEK293 cells overexpressing either one or both BK subunits and stimulated with 11,12-EET (10 µmol/L) for 2–10 minutes. The graph summarizes data from 6 independent experiments; *P<0.05 versus the unstimulated control (CTL).
The Fap Bkα Construct, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/pmc03306404-34-1-15?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
the fap-bkα construct - by Bioz Stars, 2026-08
90/100 stars
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c-terminal bk α-subunit splice variants  (STREX Inc)
90
STREX Inc c-terminal bk α-subunit splice variants
Representative blot and densitometric analysis showing the co-precipitation of <t>BKα</t> <t>with</t> <t>BKβ1</t> from HEK293 cells overexpressing either one or both BK subunits and stimulated with 11,12-EET (10 µmol/L) for 2–10 minutes. The graph summarizes data from 6 independent experiments; *P<0.05 versus the unstimulated control (CTL).
C Terminal Bk α Subunit Splice Variants, supplied by STREX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/pmc02887993-9-5-12?v=STREX+Inc
Average 90 stars, based on 1 article reviews
c-terminal bk α-subunit splice variants - by Bioz Stars, 2026-08
90/100 stars
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anti–bk α c-terminal epitope antibody  (Becton Dickinson)
90
Becton Dickinson anti–bk α c-terminal epitope antibody
Intra–α subunit disulfide cross-linking from S0 to S1 through S4. (A) Membrane topology of BK α and β1 showing residues mutated to Cys in the predicted first helical turns within the membrane and in the extracellular flanks of the TM helices. The HRV-3C protease cleavage site in the S0–S1 loop is shown as a break. (B) Strategy to determine the extent of disulfide bond formation between S0 and S1 through S4 using HRV-3C protease and DTT. (C–F) The cell surface–expressed double-Cys mutant of α indicated at the top of each immunoblot was treated with HRV-3C protease alone (first lane) or protease followed by DTT (second lane). The immunoblots were developed with an antibody against a C-terminal <t>epitope</t> of α. The extent of cross-linking is indicated under each blot.
Anti–Bk α C Terminal Epitope Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/pmc02860586-30-20-25?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
anti–bk α c-terminal epitope antibody - by Bioz Stars, 2026-08
90/100 stars
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bkα-strex/β 1  (STREX Inc)
90
STREX Inc bkα-strex/β 1
Intra–α subunit disulfide cross-linking from S0 to S1 through S4. (A) Membrane topology of BK α and β1 showing residues mutated to Cys in the predicted first helical turns within the membrane and in the extracellular flanks of the TM helices. The HRV-3C protease cleavage site in the S0–S1 loop is shown as a break. (B) Strategy to determine the extent of disulfide bond formation between S0 and S1 through S4 using HRV-3C protease and DTT. (C–F) The cell surface–expressed double-Cys mutant of α indicated at the top of each immunoblot was treated with HRV-3C protease alone (first lane) or protease followed by DTT (second lane). The immunoblots were developed with an antibody against a C-terminal <t>epitope</t> of α. The extent of cross-linking is indicated under each blot.
Bkα Strex/β 1, supplied by STREX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/pmc04838068-263-9-9?v=STREX+Inc
Average 90 stars, based on 1 article reviews
bkα-strex/β 1 - by Bioz Stars, 2026-08
90/100 stars
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bk α-subunits  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare bk α-subunits
Intra–α subunit disulfide cross-linking from S0 to S1 through S4. (A) Membrane topology of BK α and β1 showing residues mutated to Cys in the predicted first helical turns within the membrane and in the extracellular flanks of the TM helices. The HRV-3C protease cleavage site in the S0–S1 loop is shown as a break. (B) Strategy to determine the extent of disulfide bond formation between S0 and S1 through S4 using HRV-3C protease and DTT. (C–F) The cell surface–expressed double-Cys mutant of α indicated at the top of each immunoblot was treated with HRV-3C protease alone (first lane) or protease followed by DTT (second lane). The immunoblots were developed with an antibody against a C-terminal <t>epitope</t> of α. The extent of cross-linking is indicated under each blot.
Bk α Subunits, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/pmc04969794-435-62-29?v=Johns+Hopkins+HealthCare
Average 90 stars, based on 1 article reviews
bk α-subunits - by Bioz Stars, 2026-08
90/100 stars
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ab toward the extracellular region of the bkα channel (ensgdpwenfqnnqc) antibody  (GenScript corporation)
90
GenScript corporation ab toward the extracellular region of the bkα channel (ensgdpwenfqnnqc) antibody
Intra–α subunit disulfide cross-linking from S0 to S1 through S4. (A) Membrane topology of BK α and β1 showing residues mutated to Cys in the predicted first helical turns within the membrane and in the extracellular flanks of the TM helices. The HRV-3C protease cleavage site in the S0–S1 loop is shown as a break. (B) Strategy to determine the extent of disulfide bond formation between S0 and S1 through S4 using HRV-3C protease and DTT. (C–F) The cell surface–expressed double-Cys mutant of α indicated at the top of each immunoblot was treated with HRV-3C protease alone (first lane) or protease followed by DTT (second lane). The immunoblots were developed with an antibody against a C-terminal <t>epitope</t> of α. The extent of cross-linking is indicated under each blot.
Ab Toward The Extracellular Region Of The Bkα Channel (Ensgdpwenfqnnqc) Antibody, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/pmc03496203-104-8-16?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
ab toward the extracellular region of the bkα channel (ensgdpwenfqnnqc) antibody - by Bioz Stars, 2026-08
90/100 stars
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anti-bk-α-c-terminus antibody  (Becton Dickinson)
90
Becton Dickinson anti-bk-α-c-terminus antibody
Intra–α subunit disulfide cross-linking from S0 to S1 through S4. (A) Membrane topology of BK α and β1 showing residues mutated to Cys in the predicted first helical turns within the membrane and in the extracellular flanks of the TM helices. The HRV-3C protease cleavage site in the S0–S1 loop is shown as a break. (B) Strategy to determine the extent of disulfide bond formation between S0 and S1 through S4 using HRV-3C protease and DTT. (C–F) The cell surface–expressed double-Cys mutant of α indicated at the top of each immunoblot was treated with HRV-3C protease alone (first lane) or protease followed by DTT (second lane). The immunoblots were developed with an antibody against a C-terminal <t>epitope</t> of α. The extent of cross-linking is indicated under each blot.
Anti Bk α C Terminus Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/pmc02737688-64-14-16?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
anti-bk-α-c-terminus antibody - by Bioz Stars, 2026-08
90/100 stars
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bkα-myc plasmid  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare bkα-myc plasmid
Intra–α subunit disulfide cross-linking from S0 to S1 through S4. (A) Membrane topology of BK α and β1 showing residues mutated to Cys in the predicted first helical turns within the membrane and in the extracellular flanks of the TM helices. The HRV-3C protease cleavage site in the S0–S1 loop is shown as a break. (B) Strategy to determine the extent of disulfide bond formation between S0 and S1 through S4 using HRV-3C protease and DTT. (C–F) The cell surface–expressed double-Cys mutant of α indicated at the top of each immunoblot was treated with HRV-3C protease alone (first lane) or protease followed by DTT (second lane). The immunoblots were developed with an antibody against a C-terminal <t>epitope</t> of α. The extent of cross-linking is indicated under each blot.
Bkα Myc Plasmid, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/pm38873928-242-1-11?v=Johns+Hopkins+HealthCare
Average 90 stars, based on 1 article reviews
bkα-myc plasmid - by Bioz Stars, 2026-08
90/100 stars
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bkα  (STREX Inc)
90
STREX Inc bkα
Intra–α subunit disulfide cross-linking from S0 to S1 through S4. (A) Membrane topology of BK α and β1 showing residues mutated to Cys in the predicted first helical turns within the membrane and in the extracellular flanks of the TM helices. The HRV-3C protease cleavage site in the S0–S1 loop is shown as a break. (B) Strategy to determine the extent of disulfide bond formation between S0 and S1 through S4 using HRV-3C protease and DTT. (C–F) The cell surface–expressed double-Cys mutant of α indicated at the top of each immunoblot was treated with HRV-3C protease alone (first lane) or protease followed by DTT (second lane). The immunoblots were developed with an antibody against a C-terminal <t>epitope</t> of α. The extent of cross-linking is indicated under each blot.
Bkα, supplied by STREX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/10__1113_slash_jp285007-113-10-1?v=STREX+Inc
Average 90 stars, based on 1 article reviews
bkα - by Bioz Stars, 2026-08
90/100 stars
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anti-slo1-bkalpha potassium channel antibody  (Antibodies Inc)
91
Antibodies Inc anti-slo1-bkalpha potassium channel antibody
Intra–α subunit disulfide cross-linking from S0 to S1 through S4. (A) Membrane topology of BK α and β1 showing residues mutated to Cys in the predicted first helical turns within the membrane and in the extracellular flanks of the TM helices. The HRV-3C protease cleavage site in the S0–S1 loop is shown as a break. (B) Strategy to determine the extent of disulfide bond formation between S0 and S1 through S4 using HRV-3C protease and DTT. (C–F) The cell surface–expressed double-Cys mutant of α indicated at the top of each immunoblot was treated with HRV-3C protease alone (first lane) or protease followed by DTT (second lane). The immunoblots were developed with an antibody against a C-terminal <t>epitope</t> of α. The extent of cross-linking is indicated under each blot.
Anti Slo1 Bkalpha Potassium Channel Antibody, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk%CE%B1/antibodies+inc___75-408?v=Antibodies+Inc
Average 91 stars, based on 1 article reviews
anti-slo1-bkalpha potassium channel antibody - by Bioz Stars, 2026-08
91/100 stars
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Image Search Results


Representative blot and densitometric analysis showing the co-precipitation of BKα with BKβ1 from HEK293 cells overexpressing either one or both BK subunits and stimulated with 11,12-EET (10 µmol/L) for 2–10 minutes. The graph summarizes data from 6 independent experiments; *P<0.05 versus the unstimulated control (CTL).

Journal: PLoS ONE

Article Title: 11,12-EET Stimulates the Association of BK Channel α and β 1 Subunits in Mitochondria to Induce Pulmonary Vasoconstriction

doi: 10.1371/journal.pone.0046065

Figure Lengend Snippet: Representative blot and densitometric analysis showing the co-precipitation of BKα with BKβ1 from HEK293 cells overexpressing either one or both BK subunits and stimulated with 11,12-EET (10 µmol/L) for 2–10 minutes. The graph summarizes data from 6 independent experiments; *P<0.05 versus the unstimulated control (CTL).

Article Snippet: HEK293 cells were purchased from ATCC and transfected with plasmids encoding the flag-tagged BKα subunit (pM2AH-hSlo1 , addgene, Cambridge, MA) and/or the BKβ1 subunit (pCR3-hMaxiK β1; kindly provided by Annelies Janssens, KU Leuven, Leuven Belgium) using Lipofectamin™ (Invitrogen, Darmstadt, Germany).

Techniques:

Intra–α subunit disulfide cross-linking from S0 to S1 through S4. (A) Membrane topology of BK α and β1 showing residues mutated to Cys in the predicted first helical turns within the membrane and in the extracellular flanks of the TM helices. The HRV-3C protease cleavage site in the S0–S1 loop is shown as a break. (B) Strategy to determine the extent of disulfide bond formation between S0 and S1 through S4 using HRV-3C protease and DTT. (C–F) The cell surface–expressed double-Cys mutant of α indicated at the top of each immunoblot was treated with HRV-3C protease alone (first lane) or protease followed by DTT (second lane). The immunoblots were developed with an antibody against a C-terminal epitope of α. The extent of cross-linking is indicated under each blot.

Journal: The Journal of General Physiology

Article Title: Location of modulatory β subunits in BK potassium channels

doi: 10.1085/jgp.201010417

Figure Lengend Snippet: Intra–α subunit disulfide cross-linking from S0 to S1 through S4. (A) Membrane topology of BK α and β1 showing residues mutated to Cys in the predicted first helical turns within the membrane and in the extracellular flanks of the TM helices. The HRV-3C protease cleavage site in the S0–S1 loop is shown as a break. (B) Strategy to determine the extent of disulfide bond formation between S0 and S1 through S4 using HRV-3C protease and DTT. (C–F) The cell surface–expressed double-Cys mutant of α indicated at the top of each immunoblot was treated with HRV-3C protease alone (first lane) or protease followed by DTT (second lane). The immunoblots were developed with an antibody against a C-terminal epitope of α. The extent of cross-linking is indicated under each blot.

Article Snippet: The unreduced and the reduced portions were electrophoresed and transferred to nitrocellulose. α-Containing components were visualized by sequential treatment with anti–BK α C-terminal epitope antibody (BD), horseradish peroxidase (HRP)-conjugated secondary antibody, and ECL reagent (Thermo Scientific).

Techniques: Mutagenesis, Western Blot