Journal: Frontiers in Pharmacology

Article Title: Activity of Angelica sinensis extract for cutaneous applications: antioxidant, anti-senescent, and antimicrobial effects

doi: 10.3389/fphar.2026.1779635

Figure Lengend Snippet: AS does not decrease viability in HaCaT keratinocytes and BJ-5ta fibroblasts after 24 h. (A) HaCaT and (B) BJ-5ta cells were treated with AS (10, 30, 50 μg/mL) for 24 h, and cell viability was assessed by the MTT assay. Data are shown as % viability relative to untreated control (set to 100%). Data are mean ± SD of n = 3 independent biological replicates. Statistical analysis: one-way ANOVA with Dunnett’s post hoc test vs . control. **p < 0.01 vs . untreated control.

Article Snippet: BJ-5ta (Human dermal fibroblasts) cells (ATCC, LGC Standards, Milano, Italy) were cultured in 4:1 mixture of High Glucose Dulbecco’s modified Eagle’s medium (DMEM) and Medium 199 (Corning, Corning, New York, United States) supplemented with 10% fetal bovine serum (Life-Technologies, Waltham, Massachusetts, United States), and 100 U/mL penicillin, and 100 μg/mL streptomycin (Lonza, Basilea, Switzerland).

Techniques: MTT Assay, Control

Journal: Frontiers in Pharmacology

Article Title: Activity of Angelica sinensis extract for cutaneous applications: antioxidant, anti-senescent, and antimicrobial effects

doi: 10.3389/fphar.2026.1779635

Figure Lengend Snippet: AS modulates intracellular ROS under basal and H 2 O 2 -challenged conditions. HaCaT (A,B) and BJ-5ta (C,D) cells were treated with AS (10, 30, 50 μg/mL) for 3 h (A,C) or 24 h (B,D) . ROS was measured by DCF-DA fluorescence under basal conditions (left panels) and following oxidative challenge with H 2 O 2 (right panels). Data are expressed as RFU normalized to the basal control or H 2 O 2 . Data are mean ± SD from n = 3 independent biological replicates. Statistical analysis: one-way ANOVA with Dunnett’s post hoc test vs . control. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, treatment vs . control; # p < 0.05, ## p < 0.01, ### p < 0.001, H 2 O 2 vs . basal control.

Article Snippet: BJ-5ta (Human dermal fibroblasts) cells (ATCC, LGC Standards, Milano, Italy) were cultured in 4:1 mixture of High Glucose Dulbecco’s modified Eagle’s medium (DMEM) and Medium 199 (Corning, Corning, New York, United States) supplemented with 10% fetal bovine serum (Life-Technologies, Waltham, Massachusetts, United States), and 100 U/mL penicillin, and 100 μg/mL streptomycin (Lonza, Basilea, Switzerland).

Techniques: Fluorescence, Control

Journal: Frontiers in Pharmacology

Article Title: Activity of Angelica sinensis extract for cutaneous applications: antioxidant, anti-senescent, and antimicrobial effects

doi: 10.3389/fphar.2026.1779635

Figure Lengend Snippet: Scratch-wound closure in keratinocytes and fibroblasts following AS treatment. (A,B) Representative scratch images in HaCaT keratinocytes (A; 10x ) and BJ-5ta fibroblasts (B; 4x ) treated with AS (30 μg/mL). (C,D) Quantification of scratch closure in HaCaT (C) and BJ-5ta (D) . Wound closure was calculated as closure = (Area_t0 − Area_t)/Area_t0. Data are mean ± SD from n = 3 independent biological replicates. Statistical analysis: unpaired two-tailed Student’s t-test vs . control.

Article Snippet: BJ-5ta (Human dermal fibroblasts) cells (ATCC, LGC Standards, Milano, Italy) were cultured in 4:1 mixture of High Glucose Dulbecco’s modified Eagle’s medium (DMEM) and Medium 199 (Corning, Corning, New York, United States) supplemented with 10% fetal bovine serum (Life-Technologies, Waltham, Massachusetts, United States), and 100 U/mL penicillin, and 100 μg/mL streptomycin (Lonza, Basilea, Switzerland).

Techniques: Two Tailed Test, Control

Journal: Frontiers in Pharmacology

Article Title: Activity of Angelica sinensis extract for cutaneous applications: antioxidant, anti-senescent, and antimicrobial effects

doi: 10.3389/fphar.2026.1779635

Figure Lengend Snippet: AS increases COL1A1 signal in BJ-5ta fibroblasts. BJ-5ta cells were treated for 24 h with AS (30 μg/mL) or ascorbic acid (AA; 100 µM) as a positive control. (A) Representative immunofluorescence images stained for COL1A1 (green) and nuclei (Hoechst, blue). (B) Quantification of COL1A1 fluorescence expressed as mean fluorescence intensity per cell (normalized to nuclei count) and reported relative to untreated control. Data are mean ± SD from n = 2 (for AA) or 3 (for AS) independent biological replicates. Statistical analysis: unpaired two-tailed Student’s t-test vs . control. ** p < 0.01, treated vs . control.

Article Snippet: BJ-5ta (Human dermal fibroblasts) cells (ATCC, LGC Standards, Milano, Italy) were cultured in 4:1 mixture of High Glucose Dulbecco’s modified Eagle’s medium (DMEM) and Medium 199 (Corning, Corning, New York, United States) supplemented with 10% fetal bovine serum (Life-Technologies, Waltham, Massachusetts, United States), and 100 U/mL penicillin, and 100 μg/mL streptomycin (Lonza, Basilea, Switzerland).

Techniques: Positive Control, Immunofluorescence, Staining, Fluorescence, Control, Two Tailed Test

Journal: Frontiers in Pharmacology

Article Title: Activity of Angelica sinensis extract for cutaneous applications: antioxidant, anti-senescent, and antimicrobial effects

doi: 10.3389/fphar.2026.1779635

Figure Lengend Snippet: AS reduces UVB-induced senescence markers in keratinocytes and fibroblasts. (A–C) Representative SA-β-gal staining images (bright-field, top) with nuclear counterstain (Hoechst, bottom) in HaCaT (A) and BJ-5ta (C) cells under basal conditions, UVB, and UVB + AS (30 μg/mL). (B,D) Quantification of SA-β-gal–positive cells expressed as % of total cells per field in HaCaT (B) and BJ-5ta (D) Data are mean ± SD of n = 5 independent biological replicates. (E) CDKN1A (p21) mRNA expression measured by RT-qPCR and reported as relative expression (2 −ΔΔCt ) normalized to β-actin in BJ-5ta cells. Data are mean ± SD from n = 2 independent biological replicates. Statistical analysis: one-way ANOVA with Dunnett’s post hoc test vs . control. *** p < 0.001, **** p < 0.0001, treatment vs . UVB; ## p < 0.01 UVB vs . basal control.

Article Snippet: BJ-5ta (Human dermal fibroblasts) cells (ATCC, LGC Standards, Milano, Italy) were cultured in 4:1 mixture of High Glucose Dulbecco’s modified Eagle’s medium (DMEM) and Medium 199 (Corning, Corning, New York, United States) supplemented with 10% fetal bovine serum (Life-Technologies, Waltham, Massachusetts, United States), and 100 U/mL penicillin, and 100 μg/mL streptomycin (Lonza, Basilea, Switzerland).

Techniques: Staining, Expressing, Quantitative RT-PCR, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Positive feedback loop of FAM83A/PI3K/AKT/c-Jun induces migration, invasion and metastasis in hepatocellular carcinoma.

doi: 10.1016/j.biopha.2019.109780

Figure Lengend Snippet: Fig. 1. FAM83A was overexpressed in HCC. A: FAM83A mRNA levels in normal hepatocytes and HCC cell lines based on the TCGA (http://ualcan.path.uab.edu/ index.html) database. B: Western blot analysis of FAM83A expression levels in normal hepatocytes and HCC cell lines. C: FAM83A expression was measured via immunohistochemical staining in HCC and paracarcinoma tissues. D, E: Kaplan-Meier survival analysis for overall survival and progression-free survival of 82 HCC patients based on the FAM83A expression data. All experiments were performed in triplicate.

Article Snippet: Diluted primary antibody of anti-rabbit FAM83A (1:50; Cat. No. 20618-1-AP, Proteintech, Chicago, USA) was used.

Techniques: Western Blot, Expressing, Immunohistochemical staining, Staining

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Positive feedback loop of FAM83A/PI3K/AKT/c-Jun induces migration, invasion and metastasis in hepatocellular carcinoma.

doi: 10.1016/j.biopha.2019.109780

Figure Lengend Snippet: Fig. 2. Overexpression of FAM83A promoted HCC cell invasion and migration in vitro. A: Representative images of HCC cells stably transfected with fluorescently labeled lentivirus under bright-field and fluorescence microscopy. Scale bar: 250 μm. B-G: Both Bel-7404 and SMMC-7721 cells were transfected with negative control (NC) lentivirus or FAM83A lentivirus. B: RT-qPCR analysis of FAM83A mRNA expression. C: Protein levels of FAM83A, PI3K, AKT, p-PI3K, p-AKT, c-JUN, E- cadherin, N-cadherin and Vimentin were analyzed via western blot. D, E: A wound-healing assay was performed to analyze the migratory abilities of Bel-7404 and SMMC-7721 cells for 0, 24, 48 or 72 h and detected via wound-healing assays. Scale bar: 250 μm. F, G: Migration and invasion abilities of Bel-7404 and SMMC-772 were determined using transwell and Boyden chamber assays. Scale bar: 250 μm. Data are presented as mean ± s.d. from three independent experiments. *P < 0.05 versus control; **P < 0.01; ***P < 0.001.

Article Snippet: Diluted primary antibody of anti-rabbit FAM83A (1:50; Cat. No. 20618-1-AP, Proteintech, Chicago, USA) was used.

Techniques: Over Expression, Migration, In Vitro, Stable Transfection, Transfection, Labeling, Microscopy, Negative Control, Quantitative RT-PCR, Expressing, Western Blot, Wound Healing Assay, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Positive feedback loop of FAM83A/PI3K/AKT/c-Jun induces migration, invasion and metastasis in hepatocellular carcinoma.

doi: 10.1016/j.biopha.2019.109780

Figure Lengend Snippet: Fig. 3. FAM83A silencing by siRNAs inhibited HCC cell invasion and migration in vitro. A-F: Both Bel-7404 and SMMC-7721 cells were transfected with NC siRNA or FAM83A siRNA. A: RT-qPCR analysis of FAM83A mRNA expression. B: Protein levels of FAM83A, PI3K, AKT, p-PI3K, p-AKT, c-JUN, E-cadherin, N-cadherin and Vimentin were analyzed via western blot. C, D: A wound-healing assay was performed to analyze the migration abilities of Bel-7404 and SMMC-7721 cells for 0, 24, 48 or 72 h and detected via wound-healing assays. Scale bar: 250 μm. E, F: Migration and invasion abilities of Bel-7404 and SMMC-7721 were determined via transwell and Boyden chamber assays. Scale bar: 250 μm. Data are presented as mean ± s.d. from three independent experiments. *P < 0.05 versus control; **P < 0.01; ***P < 0.001.

Article Snippet: Diluted primary antibody of anti-rabbit FAM83A (1:50; Cat. No. 20618-1-AP, Proteintech, Chicago, USA) was used.

Techniques: Migration, In Vitro, Transfection, Quantitative RT-PCR, Expressing, Western Blot, Wound Healing Assay, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Positive feedback loop of FAM83A/PI3K/AKT/c-Jun induces migration, invasion and metastasis in hepatocellular carcinoma.

doi: 10.1016/j.biopha.2019.109780

Figure Lengend Snippet: Fig. 4. FAM83A induced sorafenib resistance. A, C: Bel-7404 and SMMC-7721 cells were transfected with NC lentivirus or FAM83A lentivirus. Sorafenib IC50 values of the HCC cell lines. B, D: Bel-7404 and SMMC-7721 cells were transfected with NC siRNA or FAM83A siRNA. Sorafenib IC50 values of the HCC cell lines. Data are presented as mean ± s.d. from three independent experiments. *P < 0.05 versus control; **P < 0.01; ***P < 0.001.

Article Snippet: Diluted primary antibody of anti-rabbit FAM83A (1:50; Cat. No. 20618-1-AP, Proteintech, Chicago, USA) was used.

Techniques: Transfection, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Positive feedback loop of FAM83A/PI3K/AKT/c-Jun induces migration, invasion and metastasis in hepatocellular carcinoma.

doi: 10.1016/j.biopha.2019.109780

Figure Lengend Snippet: Fig. 5. FAM83A promoted metastatic capacity of HCC cells in vivo. A: The pulmonary metastasis model was adopted to evaluate the effect of FAM83A on HCC cell metastasis. B: The number of mice with pulmonary metastasis was calculated in each group. C: Statistics of pulmonary metastatic nodules. *P < 0.05 versus control. D: Representative pictures of lung metastatic nodules under bright-field microscopy (BF), fluorescence microscopy showing green fluorescent protein expression (GFP) and H&E staining. Scale bar: 2 mm.

Article Snippet: Diluted primary antibody of anti-rabbit FAM83A (1:50; Cat. No. 20618-1-AP, Proteintech, Chicago, USA) was used.

Techniques: In Vivo, Control, Microscopy, Expressing, Staining

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Positive feedback loop of FAM83A/PI3K/AKT/c-Jun induces migration, invasion and metastasis in hepatocellular carcinoma.

doi: 10.1016/j.biopha.2019.109780

Figure Lengend Snippet: Fig. 6. c-JUN promoted FAM83A expression by binding to its promoter region. A: Schematic diagram of the promoter regions of FAM83A with the putative c-JUN transcription factor-binding sites (P1–P4). b–c Bel-7404 and SMMC-7721 cells were transfected with NC or c-JUN plasmids. B: The mRNA levels of c-JUN and FAM83A were measured via RT-qPCR. C: The protein levels of c-JUN and FAM83A were measured via western blot. D: Amplification of the c-JUN-binding sites, P1 and P3, after ChIP using c-JUN antibody. E: EMSA and supershift assays of c-JUN binding to the FAM83A promoter in Bel-7404 and SMMC-7721 cells. The free probe of labeled c-JUN was run in lane 1 as a control. A 100-fold excess of unlabeled c-JUN-WT was used to compete with c-JUN binding (lane 2 compared with lane 6). A 100-fold excess of unlabeled mutated c-JUN-P1, c-JUN-P3 or c-JUN-P1+P3 was used to compete with binding of the respective labeled probes (lanes 3–5 compared with lane 6). A supershift assay (lane 7) was performed using an anti-c-JUN antibody. F: FAM83A activated the PI3K/AKT signal pathway and induced EMT signaling, further promoting migration, invasion, metastasis and sorafenib resistance in HCC cells. c-JUN, a downstream protein of the PI3K/AKT pathway, promoted FAM83A expression by binding to the FAM83A promoter region. Data are presented as mean ± s.d. from three independent experiments. *P < 0.05 versus control; **P < 0.01; ***P < 0.001.

Article Snippet: Diluted primary antibody of anti-rabbit FAM83A (1:50; Cat. No. 20618-1-AP, Proteintech, Chicago, USA) was used.

Techniques: Expressing, Binding Assay, Transfection, Quantitative RT-PCR, Western Blot, Labeling, Control, Migration