Journal:

Article Title: Secreted Respiratory Syncytial Virus G Glycoprotein Induces Interleukin-5 (IL-5), IL-13, and Eosinophilia by an IL-4-Independent Mechanism

doi:

Figure Lengend Snippet: Cytokine and chemokine protein levels after RSV infection of G-primed and 11.B11-treated mice. Concentrations of IL-4, IL-5, IL-13, and eotaxin in lung supernatants were measured 4 days postchallenge by ELISA. The limits of detection are 20 pg of IL-4, IL-5, or IL-13 per ml and 40 ng of eotaxin per ml. The data represent means ± SEMs for data from two experiments (n = 10 for each group). ∗, significant increases comparing vac-lac- and vvGs-primed PBS-treated control mice; ∗∗, significant decreases comparing PBS- and 11.B11-treated vvGs primed mice.

Article Snippet: Bound eotaxin was detected with biotinylated polyclonal goat anti-mouse eotaxin (R & D Systems, Inc.) diluted to 0.5 μg/ml in PBS-Tween followed by horseradish peroxidase-conjugated streptavidin (Jackson ImmunoResearch, West Grove, Pa.).

Techniques: Infection, Enzyme-linked Immunosorbent Assay