biotinylated Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Vector Laboratories biotinylated secondary antibodies
    Biotinylated Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 10949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/Vector Laboratories
    Average 99 stars, based on 10949 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher biotinylated dextran amine
    Biotinylated Dextran Amine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 929 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated dextran amine/product/Thermo Fisher
    Average 99 stars, based on 929 article reviews
    Price from $9.99 to $1999.99
    biotinylated dextran amine - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories avidin biotin peroxidase complex
    Avidin Biotin Peroxidase Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 11339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex/product/Vector Laboratories
    Average 99 stars, based on 11339 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated goat anti rabbit igg
    Biotinylated Goat Anti Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 10626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rabbit igg/product/Vector Laboratories
    Average 99 stars, based on 10626 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit igg - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories avidin biotin blocking kit
    Avidin Biotin Blocking Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 10504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin blocking kit/product/Vector Laboratories
    Average 99 stars, based on 10504 article reviews
    Price from $9.99 to $1999.99
    avidin biotin blocking kit - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    94
    Agilent technologies biotinylated secondary antibody
    Comparison of the morphology of Bio-ADSCs on Avi-β-TCP and untreated ADSCs on β-TCP. Scanning electron microscopy micrographs of (A) ADSCs/β-TCP and (B) Bio-ADSCs/Avi-β-TCP constructs following 7 day incubation (magnification, ×1,200). ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, <t>biotinylated</t> ADSCs; Avi-β-TCP, avidin-coated β-TCP.
    Biotinylated Secondary Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 5121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody/product/Agilent technologies
    Average 94 stars, based on 5121 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc anti rabbit igg
    Comparison of the morphology of Bio-ADSCs on Avi-β-TCP and untreated ADSCs on β-TCP. Scanning electron microscopy micrographs of (A) ADSCs/β-TCP and (B) Bio-ADSCs/Avi-β-TCP constructs following 7 day incubation (magnification, ×1,200). ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, <t>biotinylated</t> ADSCs; Avi-β-TCP, avidin-coated β-TCP.
    Anti Rabbit Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 6668 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit igg/product/Cell Signaling Technology Inc
    Average 99 stars, based on 6668 article reviews
    Price from $9.99 to $1999.99
    anti rabbit igg - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc p38 mapk
    EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by <t>P38-siRNAs.</t> A , B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 <t>MAPK</t> (Si-P38). C , D TNF-α and IL-6 concentration were relative low in control ( C ) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (* P
    P38 Mapk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 7320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p38 mapk/product/Cell Signaling Technology Inc
    Average 99 stars, based on 7320 article reviews
    Price from $9.99 to $1999.99
    p38 mapk - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories avidin biotin complex
    EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by <t>P38-siRNAs.</t> A , B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 <t>MAPK</t> (Si-P38). C , D TNF-α and IL-6 concentration were relative low in control ( C ) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (* P
    Avidin Biotin Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 10482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex/product/Vector Laboratories
    Average 99 stars, based on 10482 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated anti mouse igg
    EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by <t>P38-siRNAs.</t> A , B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 <t>MAPK</t> (Si-P38). C , D TNF-α and IL-6 concentration were relative low in control ( C ) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (* P
    Biotinylated Anti Mouse Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 3527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti mouse igg/product/Vector Laboratories
    Average 99 stars, based on 3527 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti mouse igg - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated antibody
    EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by <t>P38-siRNAs.</t> A , B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 <t>MAPK</t> (Si-P38). C , D TNF-α and IL-6 concentration were relative low in control ( C ) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (* P
    Biotinylated Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1853 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated antibody/product/Vector Laboratories
    Average 99 stars, based on 1853 article reviews
    Price from $9.99 to $1999.99
    biotinylated antibody - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated horse anti mouse igg
    EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by <t>P38-siRNAs.</t> A , B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 <t>MAPK</t> (Si-P38). C , D TNF-α and IL-6 concentration were relative low in control ( C ) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (* P
    Biotinylated Horse Anti Mouse Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 2568 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated horse anti mouse igg/product/Vector Laboratories
    Average 99 stars, based on 2568 article reviews
    Price from $9.99 to $1999.99
    biotinylated horse anti mouse igg - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc phosphorylated akt
    EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by <t>P38-siRNAs.</t> A , B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 <t>MAPK</t> (Si-P38). C , D TNF-α and IL-6 concentration were relative low in control ( C ) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (* P
    Phosphorylated Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 4489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 4489 article reviews
    Price from $9.99 to $1999.99
    phosphorylated akt - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated goat anti rabbit igg antibody
    EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by <t>P38-siRNAs.</t> A , B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 <t>MAPK</t> (Si-P38). C , D TNF-α and IL-6 concentration were relative low in control ( C ) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (* P
    Biotinylated Goat Anti Rabbit Igg Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rabbit igg antibody/product/Vector Laboratories
    Average 99 stars, based on 1489 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit igg antibody - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc gsk3β
    EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by <t>P38-siRNAs.</t> A , B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 <t>MAPK</t> (Si-P38). C , D TNF-α and IL-6 concentration were relative low in control ( C ) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (* P
    Gsk3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 3924 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk3β/product/Cell Signaling Technology Inc
    Average 99 stars, based on 3924 article reviews
    Price from $9.99 to $1999.99
    gsk3β - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    92
    Becton Dickinson biotinylated antibodies
    CD1d distribution in lymphatic and non-lymphatic organs. ABC technique with unconjugated mAb WTH-1 unless otherwise stated. (A) LEW thymus. Bar = 500 µm. (B) Longitudinal section of LEW spleen. Hemalum counterstain. Bar = 250 µm. (C) Cross-section of LEW splenic white pulp. WTH-1 biotin-conjugated primary antibody. Bar = 250 µm. (D) C57BL/6 mouse thymus. Arrows indicate cortico-medullary boundary. Biotin-conjugated antibody. Bar = 250 µm. (E) HIS57 staining in LEW splenic white pulp. Bar = 250 µm. (F) CD4 (mAb W3/25) expression in LEW spleen. Bar = 500 µm. (G) LEW liver. Bar = 250 µm. (H) LEW heart. Hemalum counterstain. Bar = 250 µm. (I) LEW ileum. The epithelium at the tips of the villi (left rim of picture) is not preserved. <t>Biotinylated</t> WTH-1 and hemalum counterstain. Bar = 100 µm. (J) C57BL/6 ileum. Arrows indicate CD1d positive enteroendocrine cells in the epithelium. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. (K) C57BL/6 CD1d −/− ileum. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. (L) LEW pancreas. Biotinylated WTH-1. Bar = 50 µm. (M) C57BL/6 pancreas. Biotin-conjugated primary antibody, tyramide-amplified ABC technique. and hemalum counterstain. Bar = 50 µm. (N) C57BL/6 CD1d −/− pancreas. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. Abbreviations in lymphatic organs: m, medulla; c, cortex; rp, red pulp; pals, periarteriolar lymphatic sheath; mz, marginal zone and f, follicle. Abbreviations in non-lymphatic organs: c, crypts; lp, lamina propria; m, smooth muscle cells of the gut wall; i, islet of Langerhans and d, interlobular duct.
    Biotinylated Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 2195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated antibodies/product/Becton Dickinson
    Average 92 stars, based on 2195 article reviews
    Price from $9.99 to $1999.99
    biotinylated antibodies - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc rabbit anti phospho akt
    CD1d distribution in lymphatic and non-lymphatic organs. ABC technique with unconjugated mAb WTH-1 unless otherwise stated. (A) LEW thymus. Bar = 500 µm. (B) Longitudinal section of LEW spleen. Hemalum counterstain. Bar = 250 µm. (C) Cross-section of LEW splenic white pulp. WTH-1 biotin-conjugated primary antibody. Bar = 250 µm. (D) C57BL/6 mouse thymus. Arrows indicate cortico-medullary boundary. Biotin-conjugated antibody. Bar = 250 µm. (E) HIS57 staining in LEW splenic white pulp. Bar = 250 µm. (F) CD4 (mAb W3/25) expression in LEW spleen. Bar = 500 µm. (G) LEW liver. Bar = 250 µm. (H) LEW heart. Hemalum counterstain. Bar = 250 µm. (I) LEW ileum. The epithelium at the tips of the villi (left rim of picture) is not preserved. <t>Biotinylated</t> WTH-1 and hemalum counterstain. Bar = 100 µm. (J) C57BL/6 ileum. Arrows indicate CD1d positive enteroendocrine cells in the epithelium. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. (K) C57BL/6 CD1d −/− ileum. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. (L) LEW pancreas. Biotinylated WTH-1. Bar = 50 µm. (M) C57BL/6 pancreas. Biotin-conjugated primary antibody, tyramide-amplified ABC technique. and hemalum counterstain. Bar = 50 µm. (N) C57BL/6 CD1d −/− pancreas. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. Abbreviations in lymphatic organs: m, medulla; c, cortex; rp, red pulp; pals, periarteriolar lymphatic sheath; mz, marginal zone and f, follicle. Abbreviations in non-lymphatic organs: c, crypts; lp, lamina propria; m, smooth muscle cells of the gut wall; i, islet of Langerhans and d, interlobular duct.
    Rabbit Anti Phospho Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1932 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti phospho akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1932 article reviews
    Price from $9.99 to $1999.99
    rabbit anti phospho akt - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of the morphology of Bio-ADSCs on Avi-β-TCP and untreated ADSCs on β-TCP. Scanning electron microscopy micrographs of (A) ADSCs/β-TCP and (B) Bio-ADSCs/Avi-β-TCP constructs following 7 day incubation (magnification, ×1,200). ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated ADSCs; Avi-β-TCP, avidin-coated β-TCP.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration

    doi: 10.3892/etm.2015.2961

    Figure Lengend Snippet: Comparison of the morphology of Bio-ADSCs on Avi-β-TCP and untreated ADSCs on β-TCP. Scanning electron microscopy micrographs of (A) ADSCs/β-TCP and (B) Bio-ADSCs/Avi-β-TCP constructs following 7 day incubation (magnification, ×1,200). ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated ADSCs; Avi-β-TCP, avidin-coated β-TCP.

    Article Snippet: After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS.

    Techniques: Electron Microscopy, Construct, Incubation, Derivative Assay, Avidin-Biotin Assay

    (A and B) Hematoxylin and eosin staining and (C and D) immunohistochemical analysis of osteocalcin protein expression at the mandibular defect area in the (A and C) group 1 and (B and D) group 2 rabbits at 4 weeks post-operation (magnification, ×200). The group 1 and group 2 rabbits received the Bio-ADSCs/Avi-β-TCP and Bio-ADSCs/Avi-β-TCP/PRP constructs, respectively. ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated-ADSCs; Avi-β-TCP, avidin-coated β-TCP; PRP, platelet-rich plasma.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration

    doi: 10.3892/etm.2015.2961

    Figure Lengend Snippet: (A and B) Hematoxylin and eosin staining and (C and D) immunohistochemical analysis of osteocalcin protein expression at the mandibular defect area in the (A and C) group 1 and (B and D) group 2 rabbits at 4 weeks post-operation (magnification, ×200). The group 1 and group 2 rabbits received the Bio-ADSCs/Avi-β-TCP and Bio-ADSCs/Avi-β-TCP/PRP constructs, respectively. ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated-ADSCs; Avi-β-TCP, avidin-coated β-TCP; PRP, platelet-rich plasma.

    Article Snippet: After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS.

    Techniques: Staining, Immunohistochemistry, Expressing, Construct, Derivative Assay, Avidin-Biotin Assay

    Three-dimensional computed tomography images of mandibular defect reconstruction in the (A) group 1 and (B) group 2 rabbits at 4 weeks post-operation (red arrow). The group 1 and group 2 rabbits received the Bio-ADSCs/Avi-β-TCP and Bio-ADSCs/Avi-β-TCP/PRP constructs, respectively. ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated-ADSCs; Avi-β-TCP, avidin-coated β-TCP; PRP, platelet-rich plasma.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration

    doi: 10.3892/etm.2015.2961

    Figure Lengend Snippet: Three-dimensional computed tomography images of mandibular defect reconstruction in the (A) group 1 and (B) group 2 rabbits at 4 weeks post-operation (red arrow). The group 1 and group 2 rabbits received the Bio-ADSCs/Avi-β-TCP and Bio-ADSCs/Avi-β-TCP/PRP constructs, respectively. ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated-ADSCs; Avi-β-TCP, avidin-coated β-TCP; PRP, platelet-rich plasma.

    Article Snippet: After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS.

    Techniques: Computed Tomography, Construct, Derivative Assay, Avidin-Biotin Assay

    EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by P38-siRNAs. A , B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 MAPK (Si-P38). C , D TNF-α and IL-6 concentration were relative low in control ( C ) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (* P

    Journal: Journal of Translational Medicine

    Article Title: Endothelial microparticles are increased in congenital heart diseases and contribute to endothelial dysfunction

    doi: 10.1186/s12967-016-1087-2

    Figure Lengend Snippet: EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by P38-siRNAs. A , B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 MAPK (Si-P38). C , D TNF-α and IL-6 concentration were relative low in control ( C ) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (* P

    Article Snippet: Antibodies for detection of phosphorylation of eNOS at Ser1177, P38 MAPK, phosphorylation of P38 MAPK, and caveolin-1 were purchased from Cell Signaling Technology (Danvers, MA).

    Techniques: Expressing, Concentration Assay

    CD1d distribution in lymphatic and non-lymphatic organs. ABC technique with unconjugated mAb WTH-1 unless otherwise stated. (A) LEW thymus. Bar = 500 µm. (B) Longitudinal section of LEW spleen. Hemalum counterstain. Bar = 250 µm. (C) Cross-section of LEW splenic white pulp. WTH-1 biotin-conjugated primary antibody. Bar = 250 µm. (D) C57BL/6 mouse thymus. Arrows indicate cortico-medullary boundary. Biotin-conjugated antibody. Bar = 250 µm. (E) HIS57 staining in LEW splenic white pulp. Bar = 250 µm. (F) CD4 (mAb W3/25) expression in LEW spleen. Bar = 500 µm. (G) LEW liver. Bar = 250 µm. (H) LEW heart. Hemalum counterstain. Bar = 250 µm. (I) LEW ileum. The epithelium at the tips of the villi (left rim of picture) is not preserved. Biotinylated WTH-1 and hemalum counterstain. Bar = 100 µm. (J) C57BL/6 ileum. Arrows indicate CD1d positive enteroendocrine cells in the epithelium. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. (K) C57BL/6 CD1d −/− ileum. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. (L) LEW pancreas. Biotinylated WTH-1. Bar = 50 µm. (M) C57BL/6 pancreas. Biotin-conjugated primary antibody, tyramide-amplified ABC technique. and hemalum counterstain. Bar = 50 µm. (N) C57BL/6 CD1d −/− pancreas. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. Abbreviations in lymphatic organs: m, medulla; c, cortex; rp, red pulp; pals, periarteriolar lymphatic sheath; mz, marginal zone and f, follicle. Abbreviations in non-lymphatic organs: c, crypts; lp, lamina propria; m, smooth muscle cells of the gut wall; i, islet of Langerhans and d, interlobular duct.

    Journal: PLoS ONE

    Article Title: CD1d Expression in Paneth Cells and Rat Exocrine Pancreas Revealed by Novel Monoclonal Antibodies Which Differentially Affect NKT Cell Activation

    doi: 10.1371/journal.pone.0013089

    Figure Lengend Snippet: CD1d distribution in lymphatic and non-lymphatic organs. ABC technique with unconjugated mAb WTH-1 unless otherwise stated. (A) LEW thymus. Bar = 500 µm. (B) Longitudinal section of LEW spleen. Hemalum counterstain. Bar = 250 µm. (C) Cross-section of LEW splenic white pulp. WTH-1 biotin-conjugated primary antibody. Bar = 250 µm. (D) C57BL/6 mouse thymus. Arrows indicate cortico-medullary boundary. Biotin-conjugated antibody. Bar = 250 µm. (E) HIS57 staining in LEW splenic white pulp. Bar = 250 µm. (F) CD4 (mAb W3/25) expression in LEW spleen. Bar = 500 µm. (G) LEW liver. Bar = 250 µm. (H) LEW heart. Hemalum counterstain. Bar = 250 µm. (I) LEW ileum. The epithelium at the tips of the villi (left rim of picture) is not preserved. Biotinylated WTH-1 and hemalum counterstain. Bar = 100 µm. (J) C57BL/6 ileum. Arrows indicate CD1d positive enteroendocrine cells in the epithelium. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. (K) C57BL/6 CD1d −/− ileum. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. (L) LEW pancreas. Biotinylated WTH-1. Bar = 50 µm. (M) C57BL/6 pancreas. Biotin-conjugated primary antibody, tyramide-amplified ABC technique. and hemalum counterstain. Bar = 50 µm. (N) C57BL/6 CD1d −/− pancreas. Biotin-conjugated primary antibody, tyramide-amplified ABC technique and hemalum counterstain. Bar = 50 µm. Abbreviations in lymphatic organs: m, medulla; c, cortex; rp, red pulp; pals, periarteriolar lymphatic sheath; mz, marginal zone and f, follicle. Abbreviations in non-lymphatic organs: c, crypts; lp, lamina propria; m, smooth muscle cells of the gut wall; i, islet of Langerhans and d, interlobular duct.

    Article Snippet: Binding of the biotinylated antibodies was revealed by 20 min incubation with streptavidin PE-Cy5 (BD Biosciences) at 4°C.

    Techniques: Staining, Expressing, Amplification

    Characterization of two novel anti-CD1d monoclonal antibodies. (A) Titration of WTH-1 and WTH-2 mAbs on rat CD1d or mouse CD1d1 Raji transductants (left) and on C57BL/6 or LEW thymocytes (right). Cells were stained with the indicated concentrations of CD1d-specific antibodies (X axis). After washing, antibodies were detected with PE-labeled donkey anti-mouse IgG and analyzed by flow cytometry. On the Y axis, the geometric mean fluorescence intensity (MFI) is shown for the different antibody concentrations. (B) Immunoprecipitation of biotinylated surface proteins with WTH-1 (1), WTH-2 (2) or isotype control antibodies. Immunoprecipitated material was size separated under reducing conditions on a 15% SDS-PAGE, blotted onto a membrane and detected by addition of streptavidin-HRP. (C) Western blot analysis of proteins derived from rat CD1d (r) or mouse CD1d1 (m) transduced cells - left blots - and from rat tissues: spleen (spl), thymus (thy) and pancreas (pan) - right blots -. Proteins were separated on a 10% polyacrylamide gel under non-reducing conditions and blotted to a membrane. CD1d was detected with the WTH-1 or WTH-2 mAbs. The duration of film exposure for the CD1d immunoblots is indicated under the mAb names. After CD1d detection, the blots were stripped and re-probed with a polyclonal ERK2-specific antibody as protein loading control (lower blots).

    Journal: PLoS ONE

    Article Title: CD1d Expression in Paneth Cells and Rat Exocrine Pancreas Revealed by Novel Monoclonal Antibodies Which Differentially Affect NKT Cell Activation

    doi: 10.1371/journal.pone.0013089

    Figure Lengend Snippet: Characterization of two novel anti-CD1d monoclonal antibodies. (A) Titration of WTH-1 and WTH-2 mAbs on rat CD1d or mouse CD1d1 Raji transductants (left) and on C57BL/6 or LEW thymocytes (right). Cells were stained with the indicated concentrations of CD1d-specific antibodies (X axis). After washing, antibodies were detected with PE-labeled donkey anti-mouse IgG and analyzed by flow cytometry. On the Y axis, the geometric mean fluorescence intensity (MFI) is shown for the different antibody concentrations. (B) Immunoprecipitation of biotinylated surface proteins with WTH-1 (1), WTH-2 (2) or isotype control antibodies. Immunoprecipitated material was size separated under reducing conditions on a 15% SDS-PAGE, blotted onto a membrane and detected by addition of streptavidin-HRP. (C) Western blot analysis of proteins derived from rat CD1d (r) or mouse CD1d1 (m) transduced cells - left blots - and from rat tissues: spleen (spl), thymus (thy) and pancreas (pan) - right blots -. Proteins were separated on a 10% polyacrylamide gel under non-reducing conditions and blotted to a membrane. CD1d was detected with the WTH-1 or WTH-2 mAbs. The duration of film exposure for the CD1d immunoblots is indicated under the mAb names. After CD1d detection, the blots were stripped and re-probed with a polyclonal ERK2-specific antibody as protein loading control (lower blots).

    Article Snippet: Binding of the biotinylated antibodies was revealed by 20 min incubation with streptavidin PE-Cy5 (BD Biosciences) at 4°C.

    Techniques: Titration, Staining, Labeling, Flow Cytometry, Cytometry, Fluorescence, Immunoprecipitation, SDS Page, Western Blot, Derivative Assay

    CD1d and CD4 expression by MZ B cells, dendritic cells and macrophages from the spleen. One representative of three independent experiments is shown. Dot plots illustrate gating strategies. Numbers indicate the percentages of gated cells. Histograms show CD1d and CD4 expression levels. (A) MZ B cell analysis. In C57BL/6 mice, CD1d was stained with biotinylated WTH-2 mAb + SA-Cy5-PE and CD4 with RM4-APCy. In LEW, CD1d was detected with unconjugated WTH-2 mAb followed by PE-labeled donkey anti-mouse IgG antibody and CD4 with OX-35 labeled with PE-Cy5 unless otherwise indicated. Upper row histograms show gated MZ B cells, whereas, lower row histograms show total lymphocytes. MZ B cells in C57BL/6 mice were identified by gating on CD21 hi (7G6-FITC) and CD23 low/negative (B3B4-PE) cells. In LEW rats, MZ B cells were stained with two different marker combinations: CD45RA (OX-33-FITC)/HIS57-biotin + SA-APCy and IgM (G53-238-FITC)/IgD (MARD-3-biotin + SA-APCy), respectively. Analysis of CD1d and CD4 in MZ B cells defined as HIS57 and CD45RA positive cells was carried out with one single multicolor experiment. Expression of CD1d and CD4 in MZ B cells defined as IgD low and IgM high cells was determined in separated multicolor experiments as both, the CD1d and the CD4 specific antibodies, were visualized with the PE fluorochrome (CD1d: WTH-2 + PE-donkey anti-mouse IgG, CD4: OX-35-PE). (B) CD1d and CD4 expression by LEW dendritic cells (OX-62 + PE donkey anti-mouse IgG secondary antibody) and MZ B cells (HIS57-biotin and SA-APCy). CD1d was detected with WTH-2-FITC and CD4 with OX-35-PE-Cy5. (C) CD1d and CD4 expression by LEW macrophages - defined as CD11b/c + cells (OX-42-PE) - and MZ B cells (HIS57-biotin + SA-APCy). CD1d was stained with unconjugated WTH-2 followed by FITC-labeled donkey anti-mouse IgG. For CD4 detection, OX-35-PE-Cy5 antibody was used.

    Journal: PLoS ONE

    Article Title: CD1d Expression in Paneth Cells and Rat Exocrine Pancreas Revealed by Novel Monoclonal Antibodies Which Differentially Affect NKT Cell Activation

    doi: 10.1371/journal.pone.0013089

    Figure Lengend Snippet: CD1d and CD4 expression by MZ B cells, dendritic cells and macrophages from the spleen. One representative of three independent experiments is shown. Dot plots illustrate gating strategies. Numbers indicate the percentages of gated cells. Histograms show CD1d and CD4 expression levels. (A) MZ B cell analysis. In C57BL/6 mice, CD1d was stained with biotinylated WTH-2 mAb + SA-Cy5-PE and CD4 with RM4-APCy. In LEW, CD1d was detected with unconjugated WTH-2 mAb followed by PE-labeled donkey anti-mouse IgG antibody and CD4 with OX-35 labeled with PE-Cy5 unless otherwise indicated. Upper row histograms show gated MZ B cells, whereas, lower row histograms show total lymphocytes. MZ B cells in C57BL/6 mice were identified by gating on CD21 hi (7G6-FITC) and CD23 low/negative (B3B4-PE) cells. In LEW rats, MZ B cells were stained with two different marker combinations: CD45RA (OX-33-FITC)/HIS57-biotin + SA-APCy and IgM (G53-238-FITC)/IgD (MARD-3-biotin + SA-APCy), respectively. Analysis of CD1d and CD4 in MZ B cells defined as HIS57 and CD45RA positive cells was carried out with one single multicolor experiment. Expression of CD1d and CD4 in MZ B cells defined as IgD low and IgM high cells was determined in separated multicolor experiments as both, the CD1d and the CD4 specific antibodies, were visualized with the PE fluorochrome (CD1d: WTH-2 + PE-donkey anti-mouse IgG, CD4: OX-35-PE). (B) CD1d and CD4 expression by LEW dendritic cells (OX-62 + PE donkey anti-mouse IgG secondary antibody) and MZ B cells (HIS57-biotin and SA-APCy). CD1d was detected with WTH-2-FITC and CD4 with OX-35-PE-Cy5. (C) CD1d and CD4 expression by LEW macrophages - defined as CD11b/c + cells (OX-42-PE) - and MZ B cells (HIS57-biotin + SA-APCy). CD1d was stained with unconjugated WTH-2 followed by FITC-labeled donkey anti-mouse IgG. For CD4 detection, OX-35-PE-Cy5 antibody was used.

    Article Snippet: Binding of the biotinylated antibodies was revealed by 20 min incubation with streptavidin PE-Cy5 (BD Biosciences) at 4°C.

    Techniques: Expressing, Mouse Assay, Staining, Labeling, Marker

    Comparison of CD1d expression levels by rat and mouse primary cells. Representative data from one out of a total of three experiments are shown. (A) Staining of total thymocytes or splenocytes with biotinylated WTH-2 or isotype control antibodies and SA-PE. Gray and black lines correspond to C57BL/6 and LEW cells, respectively. Filled histograms are control stainings. (B) Co-expression of CD1d and TCR on thymocytes was analyzed by two-color flow cytometry. CD1d was stained using the WTH-2 mAb and PE-labeled donkey anti-mouse IgG. For staining of mouse and rat TCRs, H57-597-APCy and R73-bio + SA-APCy were used respectively. Numbers indicate the MFI of anti-CD1d mAb for the gated populations. (C) CD1d expression by B and T cells in the spleen. In C57BL/6 mice, CD1d was analyzed with the biotinylated WTH-2 mAb followed by SA-PE and in LEW rats, since biotinylated mAbs were used for B and T cell identification, CD1d was stained with unconjugated WTH-2 mAb followed by PE-labeled donkey anti-mouse IgG. Filled histograms are control stainings carried out as WTH-2 stainings but with an isotype control antibody. (Upper row) Relative CD1d expression by B and T cells. Histograms show separate multicolor experiments with same overall CD1d staining intensity ( Fig. S2 ), since both, T and B cells, were identified using APCy visualized antibodies in order to avoid unspecific signal due to fluorescence spectral overlap into the PE channel. The gating strategy and the antibodies used are explained in detail in figure S2 . Gray and black lines correspond to B and T cells, respectively. (Lower row) Histograms show CD1d expression by CD4 and CD8 positive T cells. The gating strategy and used antibodies are explained in detail in the figure S3 . Gray and black lines represent CD4 + and CD8 + T cells, respectively.

    Journal: PLoS ONE

    Article Title: CD1d Expression in Paneth Cells and Rat Exocrine Pancreas Revealed by Novel Monoclonal Antibodies Which Differentially Affect NKT Cell Activation

    doi: 10.1371/journal.pone.0013089

    Figure Lengend Snippet: Comparison of CD1d expression levels by rat and mouse primary cells. Representative data from one out of a total of three experiments are shown. (A) Staining of total thymocytes or splenocytes with biotinylated WTH-2 or isotype control antibodies and SA-PE. Gray and black lines correspond to C57BL/6 and LEW cells, respectively. Filled histograms are control stainings. (B) Co-expression of CD1d and TCR on thymocytes was analyzed by two-color flow cytometry. CD1d was stained using the WTH-2 mAb and PE-labeled donkey anti-mouse IgG. For staining of mouse and rat TCRs, H57-597-APCy and R73-bio + SA-APCy were used respectively. Numbers indicate the MFI of anti-CD1d mAb for the gated populations. (C) CD1d expression by B and T cells in the spleen. In C57BL/6 mice, CD1d was analyzed with the biotinylated WTH-2 mAb followed by SA-PE and in LEW rats, since biotinylated mAbs were used for B and T cell identification, CD1d was stained with unconjugated WTH-2 mAb followed by PE-labeled donkey anti-mouse IgG. Filled histograms are control stainings carried out as WTH-2 stainings but with an isotype control antibody. (Upper row) Relative CD1d expression by B and T cells. Histograms show separate multicolor experiments with same overall CD1d staining intensity ( Fig. S2 ), since both, T and B cells, were identified using APCy visualized antibodies in order to avoid unspecific signal due to fluorescence spectral overlap into the PE channel. The gating strategy and the antibodies used are explained in detail in figure S2 . Gray and black lines correspond to B and T cells, respectively. (Lower row) Histograms show CD1d expression by CD4 and CD8 positive T cells. The gating strategy and used antibodies are explained in detail in the figure S3 . Gray and black lines represent CD4 + and CD8 + T cells, respectively.

    Article Snippet: Binding of the biotinylated antibodies was revealed by 20 min incubation with streptavidin PE-Cy5 (BD Biosciences) at 4°C.

    Techniques: Expressing, Staining, Flow Cytometry, Cytometry, Labeling, Mouse Assay, Fluorescence