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  • 99
    Vector Laboratories avidin biotin activity
    Avidin Biotin Activity, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Thermo Fisher avidin biotin kit
    Avidin Biotin Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher biotinylated
    Analysis of the tumor antigen-specific B cell response in gastro-esophageal adenocarcinoma. LUMINEX TM analyses of 34 TAAs were performed in serum samples of untreated gastro-esophageal adenocarcinoma patients (n = 34), serum samples following neoadjuvant chemoradiotherapy (n = 7) and healthy controls (n = 5) was analyzed by LUMINEX TM (A). Individual MFIs for CTAG1A and MAGEA4 (B). Summary of antibody responses detected in serum samples of tumor patients and healthy controls (C) Exemplary flow cytometry analyses of b cells specific for NY-ESO-1 (CTAG1A) in PBMC and TDLN of a gastro-esophageal adenocarcinoma patient using <t>biotinylated</t> NY-ESO-1 and a streptavidin tetramer (D). Heatmap and bar graphs of LUMINEX TM test.
    Biotinylated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    BioLegend avidin biotin
    Analysis of the tumor antigen-specific B cell response in gastro-esophageal adenocarcinoma. LUMINEX TM analyses of 34 TAAs were performed in serum samples of untreated gastro-esophageal adenocarcinoma patients (n = 34), serum samples following neoadjuvant chemoradiotherapy (n = 7) and healthy controls (n = 5) was analyzed by LUMINEX TM (A). Individual MFIs for CTAG1A and MAGEA4 (B). Summary of antibody responses detected in serum samples of tumor patients and healthy controls (C) Exemplary flow cytometry analyses of b cells specific for NY-ESO-1 (CTAG1A) in PBMC and TDLN of a gastro-esophageal adenocarcinoma patient using <t>biotinylated</t> NY-ESO-1 and a streptavidin tetramer (D). Heatmap and bar graphs of LUMINEX TM test.
    Avidin Biotin, supplied by BioLegend, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore avidin biotin antibody complex
    Analysis of the tumor antigen-specific B cell response in gastro-esophageal adenocarcinoma. LUMINEX TM analyses of 34 TAAs were performed in serum samples of untreated gastro-esophageal adenocarcinoma patients (n = 34), serum samples following neoadjuvant chemoradiotherapy (n = 7) and healthy controls (n = 5) was analyzed by LUMINEX TM (A). Individual MFIs for CTAG1A and MAGEA4 (B). Summary of antibody responses detected in serum samples of tumor patients and healthy controls (C) Exemplary flow cytometry analyses of b cells specific for NY-ESO-1 (CTAG1A) in PBMC and TDLN of a gastro-esophageal adenocarcinoma patient using <t>biotinylated</t> NY-ESO-1 and a streptavidin tetramer (D). Heatmap and bar graphs of LUMINEX TM test.
    Avidin Biotin Antibody Complex, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Vector Laboratories biotinylated
    Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was <t>biotinylated</t> with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).
    Biotinylated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 735 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies avidin biotin blocking
    Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was <t>biotinylated</t> with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).
    Avidin Biotin Blocking, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies avidin biotin complex
    Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was <t>biotinylated</t> with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).
    Avidin Biotin Complex, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 846 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher avidin biotin complex
    Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was <t>biotinylated</t> with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).
    Avidin Biotin Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Agilent technologies avidin biotin complexes
    Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was <t>biotinylated</t> with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).
    Avidin Biotin Complexes, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Biocare Medical avidin biotin kit
    Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was <t>biotinylated</t> with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).
    Avidin Biotin Kit, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 90/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Zhongshan Golden Bridge Company avidin biotin kit
    Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was <t>biotinylated</t> with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).
    Avidin Biotin Kit, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Abcam avidin biotin complex
    Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was <t>biotinylated</t> with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).
    Avidin Biotin Complex, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Beyotime avidin biotin complex
    Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was <t>biotinylated</t> with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).
    Avidin Biotin Complex, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Analysis of the tumor antigen-specific B cell response in gastro-esophageal adenocarcinoma. LUMINEX TM analyses of 34 TAAs were performed in serum samples of untreated gastro-esophageal adenocarcinoma patients (n = 34), serum samples following neoadjuvant chemoradiotherapy (n = 7) and healthy controls (n = 5) was analyzed by LUMINEX TM (A). Individual MFIs for CTAG1A and MAGEA4 (B). Summary of antibody responses detected in serum samples of tumor patients and healthy controls (C) Exemplary flow cytometry analyses of b cells specific for NY-ESO-1 (CTAG1A) in PBMC and TDLN of a gastro-esophageal adenocarcinoma patient using biotinylated NY-ESO-1 and a streptavidin tetramer (D). Heatmap and bar graphs of LUMINEX TM test.

    Journal: Oncoimmunology

    Article Title: B cells in esophago-gastric adenocarcinoma are highly differentiated, organize in tertiary lymphoid structures and produce tumor-specific antibodies

    doi: 10.1080/2162402X.2018.1512458

    Figure Lengend Snippet: Analysis of the tumor antigen-specific B cell response in gastro-esophageal adenocarcinoma. LUMINEX TM analyses of 34 TAAs were performed in serum samples of untreated gastro-esophageal adenocarcinoma patients (n = 34), serum samples following neoadjuvant chemoradiotherapy (n = 7) and healthy controls (n = 5) was analyzed by LUMINEX TM (A). Individual MFIs for CTAG1A and MAGEA4 (B). Summary of antibody responses detected in serum samples of tumor patients and healthy controls (C) Exemplary flow cytometry analyses of b cells specific for NY-ESO-1 (CTAG1A) in PBMC and TDLN of a gastro-esophageal adenocarcinoma patient using biotinylated NY-ESO-1 and a streptavidin tetramer (D). Heatmap and bar graphs of LUMINEX TM test.

    Article Snippet: Briefly, NY-ESO-1 protein (Origene) was biotinylated (EZ-Link NHS-Biotin reagent, Thermo Fisher) and coupled to PE-conjugated streptavidin (Biolegend) in a ratio of 1:3.

    Techniques: Luminex, Flow Cytometry, Cytometry

    Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was biotinylated with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).

    Journal: BMC Biotechnology

    Article Title: Enhanced transduction of colonic cell lines in vitro and the inflamed colon in mice by viral vectors, derived from adeno-associated virus serotype 2, using virus-microbead conjugates bearing lectin

    doi: 10.1186/1472-6750-7-83

    Figure Lengend Snippet: Effect of the co-attachment of lectins to the microbead surfaces on the infectivity of AAV2-microbead conjugates . AAV2.CMV-LacZ was biotinylated with sulfo-NHS-LC-biotin at 50 μg/ml, followed by the removal of non-virion-associated biotinylation reagent by dialysis. AAV2-microbead conjugates were prepared by the attachment of biotinylated AAV2 particles to the surfaces of avidin-coated fluorescent microbeads (480 nm in diameter) (9.2 AAV2 particles per microbead). To these AAV2-microbead conjugates, a biotinylated form of each lectin was added in excess (0.2 μg biotinylated lectin per 10 7 avidin-coated microbeads), followed by the removal of unbound lectin molecules by centrifugation. The infectivity of these AAV2-microbead conjugates with and without lectin was analyzed on HeLa, COLO 205, and MIP-101 cell lines. Cells were cultured in 24-well plates at 37°C for 24 hr (initial cell number per well: HeLa, 5 × 10 4 ; COLO 205, 1 × 10 5 ; MIP-101, 7.5 × 10 4 ). AAV2-microbead conjugates bearing each lectin, along with free unmodified AAV2.CMV-LacZ, free biotinylated AAV2.CMV-LacZ, and AAV2-microbead conjugates without lectin, were applied to target cells (a total of 1 × 10 7 AAV2 particles per well) and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde and stained for β-galactosidase activity using X-gal as the substrate. Then, the number of infected cells in each well was counted under a light microscope. Each datum shown is the average number of infected cells per well with a standard deviation (n = 16). A, free, unmodified AAV2.CMV-LacZ; B, free, biotinylated AAV2.CMV-LacZ; C, AAV2-microbead conjugates without lectin; D – J, AAV2-microbead conjugates bearing lectin (D, Con A; E, horse gram agglutinin; F, peanut agglutinin; G, castor bean agglutinin I; H, soybean agglutinin; I, furze gorse agglutinin I; and J, wheat germ agglutinin).

    Article Snippet: The following lectins in biotinylated form were obtained from Vector Laboratories [the carbohydrate structure(s), to which each lectin binds, is indicated in bracket]: Con A from Jack bean (Canavalia ensiformis ) seeds [mannose]; agglutinin from horse gram (Dolichos biflorus ) seeds [N -acetylgalactosamine]; agglutinin from peanuts (Arachis hypogaea ) [galactosyl (β-1,3)N -acetylgalactosamine]; agglutinin I from castor bean (Ricinus communis ) seeds [galactose and N -acetylglucosamine]; agglutinin from soybean (Glycine max ) seeds [N -acetylgalactosamine and galactose]; agglutinin I from furze gorse (Ulex europaeus ) seeds [fucose]; and agglutinin from wheat germ (Triticum vulgaris ) [N -acetylglucosamine].

    Techniques: Infection, Avidin-Biotin Assay, Centrifugation, Cell Culture, Incubation, Staining, Activity Assay, Light Microscopy, Standard Deviation

    Infectivity analysis of AAV2 treated with sulfo-NHS-LC-biotin . AAV2.CMV-LacZ was treated with varying concentrations of sulfo-NHS-LC-biotin at room temperature for 45 min in the dark, followed by the addition of excess glycine to terminate the biotinylation reaction. The resulting biotinylated AAV2 preparations were applied to HeLa cells (5 × 10 6 AAV2 particles per well), which had been grown in 24-well plates at 37°C for 24 hr (initial cell number, 5 × 10 4 cells per well), and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde, stained for β-galactosidase (LacZ) activity using X-gal as the substrate. Then, the number of infected cells, which were stained blue, in each well was counted under a light microscope (-○-). The same analysis was also performed on biotinylated AAV2 preparations, to which excess Neutralite avidin (100 μg per 5 × 10 6 AAV2 particles) had been added (-●-). Each datum shown is the average number of infected cells per well with a standard deviation (n = 26).

    Journal: BMC Biotechnology

    Article Title: Enhanced transduction of colonic cell lines in vitro and the inflamed colon in mice by viral vectors, derived from adeno-associated virus serotype 2, using virus-microbead conjugates bearing lectin

    doi: 10.1186/1472-6750-7-83

    Figure Lengend Snippet: Infectivity analysis of AAV2 treated with sulfo-NHS-LC-biotin . AAV2.CMV-LacZ was treated with varying concentrations of sulfo-NHS-LC-biotin at room temperature for 45 min in the dark, followed by the addition of excess glycine to terminate the biotinylation reaction. The resulting biotinylated AAV2 preparations were applied to HeLa cells (5 × 10 6 AAV2 particles per well), which had been grown in 24-well plates at 37°C for 24 hr (initial cell number, 5 × 10 4 cells per well), and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde, stained for β-galactosidase (LacZ) activity using X-gal as the substrate. Then, the number of infected cells, which were stained blue, in each well was counted under a light microscope (-○-). The same analysis was also performed on biotinylated AAV2 preparations, to which excess Neutralite avidin (100 μg per 5 × 10 6 AAV2 particles) had been added (-●-). Each datum shown is the average number of infected cells per well with a standard deviation (n = 26).

    Article Snippet: The following lectins in biotinylated form were obtained from Vector Laboratories [the carbohydrate structure(s), to which each lectin binds, is indicated in bracket]: Con A from Jack bean (Canavalia ensiformis ) seeds [mannose]; agglutinin from horse gram (Dolichos biflorus ) seeds [N -acetylgalactosamine]; agglutinin from peanuts (Arachis hypogaea ) [galactosyl (β-1,3)N -acetylgalactosamine]; agglutinin I from castor bean (Ricinus communis ) seeds [galactose and N -acetylglucosamine]; agglutinin from soybean (Glycine max ) seeds [N -acetylgalactosamine and galactose]; agglutinin I from furze gorse (Ulex europaeus ) seeds [fucose]; and agglutinin from wheat germ (Triticum vulgaris ) [N -acetylglucosamine].

    Techniques: Infection, Incubation, Staining, Activity Assay, Light Microscopy, Avidin-Biotin Assay, Standard Deviation