Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells

doi: 10.1007/s00262-022-03177-6

Figure Lengend Snippet: Elo-mediated ADCC in the FcR engineered Jurkat ADCC indicator system. A BCBL-1 cells were incubated with three different concentrations of Elo, and co-cultured with ADCC-indicating Jurkat cells (E:T ratio = 1:5) for 18 h. Luciferase signal was then measured by luminometer. B GTO and TY-1 cells were incubated with 100 μg/ml of Elo, and co-cultured with ADCC-indicating Jurkat cells (E:T ratio = 1:5) for 18 h. Luciferase signal was then measured by luminometer

Article Snippet: Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay.

Techniques: Incubation, Cell Culture, Luciferase

Journal: The Journal of Infectious Diseases

Article Title: Murine Model of Maternal Immunization Demonstrates Protective Role for Antibodies That Mediate Antibody-Dependent Cellular Cytotoxicity in Protecting Neonates From Herpes Simplex Virus Type 1 and Type 2

doi: 10.1093/infdis/jiz521

Figure Lengend Snippet: Fcγ receptor (FcγR) expression and ability of neonatal and adult immune cells to mediate antibody-dependent killing of herpes simplex virus (HSV)-infected target cells. Single-cell suspensions of pooled hepatocytes and splenocytes from adult and 7-day-old neonatal mice were obtained, and FcγR expression was quantified by flow on (A) macrophages, (B) monocytes, and (C) neutrophils. Populations were defined by expression of indicated markers and results are presented as percentage of positive cells. (D) Antibody-dependent cellular killing was quantified using single-cell suspensions of immune cells from different aged pups or adults (6–8 weeks) as effectors and HSV-2(333)ZAG-infected cells as targets and pooled heat-inactivated immune serum from ΔgD-2 or VD60 (control) immunized adult mice. The results are presented as percentage of killed targets (GFP+ [HSV-2-infected] and FVD450+ [dead cells]) relative to FVD450+ dead cells in the absence of serum. Each point represents results for a single mouse, and results were compared with controls (VD60 immune serum) by analysis of variance with Tukey’s multiple comparison (**, P < .01; ***, P < .001).

Article Snippet: We thank Mei Cong, Aileen Paguio, and Vanessa Ott from Promega for providing murine Fcγ receptor activation bioassays.

Techniques: Expressing, Virus, Infection, Control, Comparison