bio_rxiv__211706 Search Results


gfp  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology gfp
A. The relative mRNA levels of IFNαRI downstream genes, ISG15 and PKR, in IFNα treated WT <t>and</t> <t>HDAC11</t> KO HAP1 cells were quantified by RT-PCR and normalized to GAPDH expression. B. The relative mRNA levels of ISG15 and PKR in IFNα treated control (Luc KD) and HDAC11 KD MCF-7 cells were quantified by RT-PCR and normalized to GAPDH expression. C. The effect of SHMT2 KD on the relative mRNA level of ISG15 and PKR in HDAC11 KO HAP1 cells. D. HDAC11 KO cells had reduced <t>GFP</t> expression compared to WT HAP1 cells when treated with IFNα and infected with lentivirus carrying a pCCL vector encoding GFP. Quantification of the GFP protein levels is shown in the lower panel. E. WT and HDAC11 KO mice were injected with VSV for 2 days and the lung and liver were harvested for mRNA analysis. The relative mRNA levels of IFNαRI downstream genes, ISG15 and PKR, were quantified by RT-PCR and normalized to GAPDH expression. Values with error bars indicate mean ± sd of three samples.
Gfp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio_rxiv__211706/m-IgG%CE%BA+BP-HRP/bio_rxiv__211706-121-8-16
Average 96 stars, based on 1 article reviews
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bca protein assay kit  (Thermo Fisher)
99
Thermo Fisher bca protein assay kit
A. The relative mRNA levels of IFNαRI downstream genes, ISG15 and PKR, in IFNα treated WT <t>and</t> <t>HDAC11</t> KO HAP1 cells were quantified by RT-PCR and normalized to GAPDH expression. B. The relative mRNA levels of ISG15 and PKR in IFNα treated control (Luc KD) and HDAC11 KD MCF-7 cells were quantified by RT-PCR and normalized to GAPDH expression. C. The effect of SHMT2 KD on the relative mRNA level of ISG15 and PKR in HDAC11 KO HAP1 cells. D. HDAC11 KO cells had reduced <t>GFP</t> expression compared to WT HAP1 cells when treated with IFNα and infected with lentivirus carrying a pCCL vector encoding GFP. Quantification of the GFP protein levels is shown in the lower panel. E. WT and HDAC11 KO mice were injected with VSV for 2 days and the lung and liver were harvested for mRNA analysis. The relative mRNA levels of IFNαRI downstream genes, ISG15 and PKR, were quantified by RT-PCR and normalized to GAPDH expression. Values with error bars indicate mean ± sd of three samples.
Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio_rxiv__211706/BCA+Protein+Assay+Kit/bio_rxiv__211706-211-8-7
Average 99 stars, based on 1 article reviews
bca protein assay kit - by Bioz Stars, 2026-10
99/100 stars
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streptavidin agarose beads  (Thermo Fisher)
99
Thermo Fisher streptavidin agarose beads
A. In-gel fluorescence showing SHMT2 is a lysine fatty-acylated protein using Alk14 labeling and fluorescence conjugation through click chemistry. B. Fatty-acylation of endogenous SHMT2 in HEK293T cells was detected using Alk14 labeling, biotin-azide conjugation, and <t>streptavidin</t> pulldown, followed by immunoblotting against SHMT2. C. SHMT2 Alk14 labeling after in vitro treatment with wild-type (WT) or the Y304H catalytic mutant (YH) of HDAC11. Quantification of the relative levels of lysine fatty-acylation was shown in the right panel. D. In-gel fluorescence showing lysine fatty-acylation of SHMT2 was regulated by WT but not the Y304H catalytic mutant of HDAC11 in HEK293T cells co-overexpressing SHMT2 and HDAC11. Quantification of the relative levels of lysine fatty-acylation was shown in the lower panel. E. The fatty-acylation of endogenous SHMT2 was increased after knocking down HDAC11 in MCF-7 cells. Quantification of the relative level of lysine fatty-acylation was shown in the lower panel. F. Schematic representation of Flag-tagged WT and truncated SHMT2 constructs. G. In-gel fluorescence showing fatty-acylation was localized to residue 203-504 of SHMT2. H. In-gel fluorescence gel showing K245 is the major fatty-acylation site of SHMT2. Quantification of the relative level of lysine fatty-acylation is shown in the right panel. In all panels, values with error bars indicate mean ± sd of three replicates.
Streptavidin Agarose Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio_rxiv__211706/Streptavidin/bio_rxiv__211706-126-0-18
Average 99 stars, based on 1 article reviews
streptavidin agarose beads - by Bioz Stars, 2026-10
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phenylmethylsulfonyl fluoride  (Enamine Ltd)
99
Enamine Ltd phenylmethylsulfonyl fluoride
A. In-gel fluorescence showing SHMT2 is a lysine fatty-acylated protein using Alk14 labeling and fluorescence conjugation through click chemistry. B. Fatty-acylation of endogenous SHMT2 in HEK293T cells was detected using Alk14 labeling, biotin-azide conjugation, and <t>streptavidin</t> pulldown, followed by immunoblotting against SHMT2. C. SHMT2 Alk14 labeling after in vitro treatment with wild-type (WT) or the Y304H catalytic mutant (YH) of HDAC11. Quantification of the relative levels of lysine fatty-acylation was shown in the right panel. D. In-gel fluorescence showing lysine fatty-acylation of SHMT2 was regulated by WT but not the Y304H catalytic mutant of HDAC11 in HEK293T cells co-overexpressing SHMT2 and HDAC11. Quantification of the relative levels of lysine fatty-acylation was shown in the lower panel. E. The fatty-acylation of endogenous SHMT2 was increased after knocking down HDAC11 in MCF-7 cells. Quantification of the relative level of lysine fatty-acylation was shown in the lower panel. F. Schematic representation of Flag-tagged WT and truncated SHMT2 constructs. G. In-gel fluorescence showing fatty-acylation was localized to residue 203-504 of SHMT2. H. In-gel fluorescence gel showing K245 is the major fatty-acylation site of SHMT2. Quantification of the relative level of lysine fatty-acylation is shown in the right panel. In all panels, values with error bars indicate mean ± sd of three replicates.
Phenylmethylsulfonyl Fluoride, supplied by Enamine Ltd, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio_rxiv__211706/Phenylmethylsulfonyl+fluoride/custom%40en300-24380%4010%2E1101%2F211706
Average 99 stars, based on 1 article reviews
phenylmethylsulfonyl fluoride - by Bioz Stars, 2026-10
99/100 stars
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Image Search Results


A. The relative mRNA levels of IFNαRI downstream genes, ISG15 and PKR, in IFNα treated WT and HDAC11 KO HAP1 cells were quantified by RT-PCR and normalized to GAPDH expression. B. The relative mRNA levels of ISG15 and PKR in IFNα treated control (Luc KD) and HDAC11 KD MCF-7 cells were quantified by RT-PCR and normalized to GAPDH expression. C. The effect of SHMT2 KD on the relative mRNA level of ISG15 and PKR in HDAC11 KO HAP1 cells. D. HDAC11 KO cells had reduced GFP expression compared to WT HAP1 cells when treated with IFNα and infected with lentivirus carrying a pCCL vector encoding GFP. Quantification of the GFP protein levels is shown in the lower panel. E. WT and HDAC11 KO mice were injected with VSV for 2 days and the lung and liver were harvested for mRNA analysis. The relative mRNA levels of IFNαRI downstream genes, ISG15 and PKR, were quantified by RT-PCR and normalized to GAPDH expression. Values with error bars indicate mean ± sd of three samples.

Journal: bioRxiv

Article Title: HDAC11 regulates type I interferon signaling through defatty-acylation of SHMT2

doi: 10.1101/211706

Figure Lengend Snippet: A. The relative mRNA levels of IFNαRI downstream genes, ISG15 and PKR, in IFNα treated WT and HDAC11 KO HAP1 cells were quantified by RT-PCR and normalized to GAPDH expression. B. The relative mRNA levels of ISG15 and PKR in IFNα treated control (Luc KD) and HDAC11 KD MCF-7 cells were quantified by RT-PCR and normalized to GAPDH expression. C. The effect of SHMT2 KD on the relative mRNA level of ISG15 and PKR in HDAC11 KO HAP1 cells. D. HDAC11 KO cells had reduced GFP expression compared to WT HAP1 cells when treated with IFNα and infected with lentivirus carrying a pCCL vector encoding GFP. Quantification of the GFP protein levels is shown in the lower panel. E. WT and HDAC11 KO mice were injected with VSV for 2 days and the lung and liver were harvested for mRNA analysis. The relative mRNA levels of IFNαRI downstream genes, ISG15 and PKR, were quantified by RT-PCR and normalized to GAPDH expression. Values with error bars indicate mean ± sd of three samples.

Article Snippet: Antibodies against HDAC11 (sc-390737), β-actin (sc-4777), SHMT2 (sc-390641), GFP (sc-8334), and HA (sc-516102) were purchased from Santa Cruz Biotechnology.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Infection, Plasmid Preparation, Injection

A. In-gel fluorescence showing SHMT2 is a lysine fatty-acylated protein using Alk14 labeling and fluorescence conjugation through click chemistry. B. Fatty-acylation of endogenous SHMT2 in HEK293T cells was detected using Alk14 labeling, biotin-azide conjugation, and streptavidin pulldown, followed by immunoblotting against SHMT2. C. SHMT2 Alk14 labeling after in vitro treatment with wild-type (WT) or the Y304H catalytic mutant (YH) of HDAC11. Quantification of the relative levels of lysine fatty-acylation was shown in the right panel. D. In-gel fluorescence showing lysine fatty-acylation of SHMT2 was regulated by WT but not the Y304H catalytic mutant of HDAC11 in HEK293T cells co-overexpressing SHMT2 and HDAC11. Quantification of the relative levels of lysine fatty-acylation was shown in the lower panel. E. The fatty-acylation of endogenous SHMT2 was increased after knocking down HDAC11 in MCF-7 cells. Quantification of the relative level of lysine fatty-acylation was shown in the lower panel. F. Schematic representation of Flag-tagged WT and truncated SHMT2 constructs. G. In-gel fluorescence showing fatty-acylation was localized to residue 203-504 of SHMT2. H. In-gel fluorescence gel showing K245 is the major fatty-acylation site of SHMT2. Quantification of the relative level of lysine fatty-acylation is shown in the right panel. In all panels, values with error bars indicate mean ± sd of three replicates.

Journal: bioRxiv

Article Title: HDAC11 regulates type I interferon signaling through defatty-acylation of SHMT2

doi: 10.1101/211706

Figure Lengend Snippet: A. In-gel fluorescence showing SHMT2 is a lysine fatty-acylated protein using Alk14 labeling and fluorescence conjugation through click chemistry. B. Fatty-acylation of endogenous SHMT2 in HEK293T cells was detected using Alk14 labeling, biotin-azide conjugation, and streptavidin pulldown, followed by immunoblotting against SHMT2. C. SHMT2 Alk14 labeling after in vitro treatment with wild-type (WT) or the Y304H catalytic mutant (YH) of HDAC11. Quantification of the relative levels of lysine fatty-acylation was shown in the right panel. D. In-gel fluorescence showing lysine fatty-acylation of SHMT2 was regulated by WT but not the Y304H catalytic mutant of HDAC11 in HEK293T cells co-overexpressing SHMT2 and HDAC11. Quantification of the relative levels of lysine fatty-acylation was shown in the lower panel. E. The fatty-acylation of endogenous SHMT2 was increased after knocking down HDAC11 in MCF-7 cells. Quantification of the relative level of lysine fatty-acylation was shown in the lower panel. F. Schematic representation of Flag-tagged WT and truncated SHMT2 constructs. G. In-gel fluorescence showing fatty-acylation was localized to residue 203-504 of SHMT2. H. In-gel fluorescence gel showing K245 is the major fatty-acylation site of SHMT2. Quantification of the relative level of lysine fatty-acylation is shown in the right panel. In all panels, values with error bars indicate mean ± sd of three replicates.

Article Snippet: Streptavidin agarose beads, ECL plus western blotting detection reagent and universal nuclease for cell lysis were purchased from Thermo Scientific Pierce.

Techniques: Fluorescence, Labeling, Conjugation Assay, Western Blot, In Vitro, Mutagenesis, Construct